全文获取类型
收费全文 | 17277篇 |
免费 | 892篇 |
国内免费 | 156篇 |
专业分类
耳鼻咽喉 | 138篇 |
儿科学 | 347篇 |
妇产科学 | 588篇 |
基础医学 | 1898篇 |
口腔科学 | 448篇 |
临床医学 | 1379篇 |
内科学 | 4406篇 |
皮肤病学 | 300篇 |
神经病学 | 1848篇 |
特种医学 | 729篇 |
外科学 | 2610篇 |
综合类 | 49篇 |
一般理论 | 1篇 |
预防医学 | 591篇 |
眼科学 | 310篇 |
药学 | 1038篇 |
中国医学 | 24篇 |
肿瘤学 | 1621篇 |
出版年
2024年 | 22篇 |
2023年 | 177篇 |
2022年 | 345篇 |
2021年 | 603篇 |
2020年 | 390篇 |
2019年 | 478篇 |
2018年 | 602篇 |
2017年 | 408篇 |
2016年 | 492篇 |
2015年 | 552篇 |
2014年 | 758篇 |
2013年 | 898篇 |
2012年 | 1377篇 |
2011年 | 1380篇 |
2010年 | 760篇 |
2009年 | 697篇 |
2008年 | 1101篇 |
2007年 | 1100篇 |
2006年 | 1058篇 |
2005年 | 960篇 |
2004年 | 924篇 |
2003年 | 791篇 |
2002年 | 679篇 |
2001年 | 155篇 |
2000年 | 161篇 |
1999年 | 173篇 |
1998年 | 154篇 |
1997年 | 113篇 |
1996年 | 116篇 |
1995年 | 104篇 |
1994年 | 72篇 |
1993年 | 63篇 |
1992年 | 93篇 |
1991年 | 91篇 |
1990年 | 52篇 |
1989年 | 54篇 |
1988年 | 42篇 |
1987年 | 42篇 |
1986年 | 41篇 |
1985年 | 32篇 |
1984年 | 38篇 |
1983年 | 31篇 |
1982年 | 27篇 |
1981年 | 20篇 |
1980年 | 10篇 |
1976年 | 8篇 |
1973年 | 7篇 |
1971年 | 8篇 |
1970年 | 9篇 |
1969年 | 7篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
71.
Ciaroni S Cecchini T Ferri P Cuppini R Ambrogini P Santi S Benedetti S Del Grande P Papa S 《Neuroscience research》2002,44(4):369-377
The adult hippocampal neurogenesis is affected by vitamin E deficiency. In the present investigation we examined if neural precursor proliferation, newborn cell survival or both are altered by vitamin E deficiency. 5-Bromo-2'-deoxyuridine (BrdU) was employed as a marker of proliferating cells. BrdU-labelled cells were revealed 1 and 30 days after BrdU administration in order to evaluate proliferation and newborn cell survival, respectively. Cell proliferation decreased in controls from juvenile to adult age, and the decrease was lesser in vitamin E deficiency. Thus we found a higher number of proliferating cells in vitamin E-deficient rats than in age-matched controls at 5 months of age. Comparing the number of BrdU-positive cells between 1 and 30 days after the last BrdU injection revealed a remarkable decrease in all groups; this is the greatest in vitamin E-deficient rats and the lowest in control rats. Consistently cell death in the dentate gyrus, assessed by TUNEL technique, was found to decrease from 1 to 5 months of age, but at 5 months it was significantly higher in vitamin E-deficient rats than in age-matched controls. These data show that vitamin E deficiency enhances neural precursor proliferation and cell death during adult neurogenesis. 相似文献
72.
Hepatocyte growth factor (HGF), a stimulator of angiogenesis and cell migration, regulates the growth of a wide variety of
cells by binding to its high-affinity receptor met and is involved in the growth and aggressiveness of several tumors. In
this study we investigated the expression of HGF and met in normal endocrine cells and related neoplasms of the gut and pancreas
to verify their possible role in tumor pathogenesis, growth, and aggressiveness. Normal tissues and 60 different endocrine
tumors were immunostained using specific antibodies directed against HGF, met, and various hormones. HGF immunoreactivity
(IR) was found in antroduodenal G cells, rectal enterochromaffin (EC) cells, and pancreatic A and B cells, whereas met IR
was detected in antral EC and G cells, and in pancreatic B cells; 46 of 60 tumors examined were positive for HGF, and they
were mainly represented by ECL-, EC-, and L-cell neoplasms. met IR was identified in 50/60 tumors of various phenotypes. HGF
and met coexpression was found in 42/60 cases, most of which were represented by EC-cell tumors. HGF/met coexpression was
significantly more frequent in ileolonic EC-cell tumors, which in the majority of cases were malignant, than in appendiceal
EC-cell tumors, which were all benign. Our results demonstrated, for the first time, that HGF and met are specifically distributed
in normal gut and pancreatic endocrine cells and, in addition, suggest that HGF and met may be implicated as autocrine/paracrine
factors regulating the growth of gastroenteropancreatic endocrine tumors, mainly of ileocolonic EC-cell carcinoids. 相似文献
73.
A MicroRNA signature associated with prognosis and progression in chronic lymphocytic leukemia 总被引:2,自引:0,他引:2
74.
75.
De Luca A Buccino A Gianni D Mangino M Giustini S Richetta A Divona L Calvieri S Mingarelli R Dallapiccola B 《Human mutation》2003,21(2):171-172
The high mutation rate at the NF1 locus results in a wide range of molecular abnormalities. The majority of these mutations are private and rare, generating elevated allelic diversity with a restricted number of recurrent mutations. In this study, we have assessed the efficacy of denaturing high-performance liquid chromatography (DHPLC), for detecting mutation in the NF1 gene. DHPLC is a fast and highly sensitive technique based on the detection of heteroduplexes in PCR products by ion pair reverse-phase HPLC under partially denaturing conditions. We established theoretical conditions for DHPLC analysis of all coding exons and splice junctions of the NF1 gene using the WAVEmaker software version 4.1.40 and screened for mutations a panel of 40 unrelated NF1 patients (25 sporadic and 15 familial), genetically uncharacterized. Disruptive mutations were identified in 29 individuals with an overall mutation detection rate of 72.5%. The mutations included eight deletions (exons 4b, 7, 10a, 14, 26, and 31), one insertion (exon 8), nine nonsense mutation (exons 10a, 13, 23.1, 27a, 29, 31, and 36), six missense mutations (exons 15, 16, 17, 24, and 31), four splice errors (exons 11, 14, 36, and 40) and a complex rearrangement within exon 16. Eighteen (62%) of the identified disruptive mutations are novel. Seven unclassified and three previously reported polymorphisms were also detected. None of the missense mutations identified in this study were found after screening of 150 controls. Our results suggest that DHPLC provides an accurate method for the rapid identification of NF1 mutations. 相似文献
76.
Bellan C De Falco G Lazzi S Micheli P Vicidomini S Schürfeld K Amato T Palumbo A Bagella L Sabattini E Bartolommei S Hummel M Pileri S Tosi P Leoncini L Giordano A 《The Journal of pathology》2004,203(4):946-952
CDK9 is a member of the CDC2-like family of kinases. Its cyclin partners are members of the CYCLIN T family (T1, T2a, and T2b) and CYCLIN K. The CDK9/CYCLIN T1 complex is very important in the differentiation programme of several cell types, controlling specific differentiation pathways. Limited data are available regarding the expression of CDK9/CYCLIN T1 in haematopoietic and lymphoid tissues. The aim of this study was to analyse the expression of the CDK9/CYCLIN T1 complex in lymphoid tissue, in order to assess its role in B- and T-cell differentiation and lymphomagenesis. CDK9/CYCLIN T1 expression was found by immunohistochemistry in precursor B and T cells. In peripheral lymphoid tissues, germinal centre cells and scattered B- and T-cell blasts in interfollicular areas expressed CDK9/CYCLIN T1, while mantle cells, plasma cells, and small resting T-lymphocytes displayed no expression of either molecule. CDK9/CYCLIN T1 expression therefore appears to be related to particular stages of lymphoid differentiation/activation. CDK9 and CYCLIN T1 were highly expressed in lymphomas derived from precursor B and T cells, from germinal centre cells, such as follicular lymphomas, and from activated T cells (ie anaplastic large cell lymphomas). Hodgkin and Reed-Sternberg cells of classical Hodgkin's lymphoma also showed strong nuclear staining. Diffuse large B-cell, Burkitt's lymphomas, and peripheral T-cell lymphomas, among T-cell lymphoproliferative disorders, showed a wide range of values. No expression of CDK9 or CYCLIN T1 was detected in mantle cell and marginal zone lymphomas. However, at the mRNA level, an imbalance in the CDK9/CYCLIN T1 ratio was found in follicular lymphoma and diffuse large B-cell lymphomas with germinal centre phenotype, and in the cell lines of classical Hodgkin's lymphomas, Burkitt's lymphomas, and anaplastic large cell lymphoma, in comparison with reactive lymph nodes. These results suggest that the CDK9/CYCLIN T1 complex may affect the activation and differentiation programme of lymphoid cells. The molecular mechanism through which the CDK9/CYCLIN T1 complex is altered in malignant transformation needs to be elucidated. 相似文献
77.
78.
A HeLa cell line stably expressing the enhanced green fluorescence protein (EGFP) gene, interrupted by the HBB IVS2‐654 intron, was studied without treatment and after treatment with a single standard dose of 15 μM of N‐methyl‐N′‐nitro‐N‐nitrosoguanidine (MNNG). This assay was done in order to prove that such a construct can revert by a variety of mechanisms and that it produces a visible phenotype, i.e., green fluorescence. The system permits visual detection of living mutant cells among a background of non‐mutant cells and does not require a selective medium. The results show that the construct reverts by large deletions (–62, –100, and –162 bp), small insertions (+4 bp), small rearrangements (19 bp duplication), base substitutions at purines (G652, G653, A655, G579), and a pyrimidine (T654) between nucleotide positions 579 and 837. Splice‐site mutations were recovered, and some of the mechanisms underlying these mutations are discussed. Because of the ease of detection of revertant cells under fluorescent light and the wide variety of mutations that can be recovered, further development of this system could make it a useful new mammalian cell mutagenicity assay. Hum Mutat 18:526–534, 2001. © 2001 Wiley‐Liss, Inc. 相似文献
79.
80.
Angelo Monteverde Marco Ballarè Stefano Pileri 《Springer Seminars in Immunopathology》1997,19(1):99-110
Summary We have examined the clinical (virological and immunological), histological and immunohistochemical features of liver lymphoid nodules in hepatitis C virus-positive (HCV+)/mixed cryoglobulinemia (type II and III) and chronic hepatitis C. The clinical features of liver disease were found to be similar in all patients. In all these groups, liver lymphoid nodules were observed to a similar extent, being more frequent in earlier phases of liver disease and less in more advanced stages. These data were confirmed by studies in serial biopsy samples taken from individual patients with type II mixed cryoglobulinemia; the loss of lymphoid nodules with progression to more advanced histological stages of disease in these patients was accompanied by a decrease of the serum levels of cryoglobulins (although not statistically significant). By immunohistochemical analysis, the liver lymphoid nodules contained predominantly B cells with a CD5+/bcl2+/Ki67– phenotype, which were always polyclonal in type III mixed cryoglobulinemia and chronic hepatitis C, and monoclonal in type II mixed cryoglobulinemia. These immunological features were consistent with an active role of the immune system in HCV-associated liver necro-inflammation. Only in type II mixed cryoglobulinemia was there a clonal restriction of B cells. The immunological profile (autoantibodies) and viral genotypes were examined in some patients, but no significant correlation with clinical and immunohistochemical findings was found; however, the prevalence of genotype 2a was significantly higher in type II mixed cryoglobulinemia than in type III and chronic hepatitis without cryoglobulinemia. 相似文献