成肌细胞自体移植对大鼠神经植入术后终板再生及神经肌肉功能恢复的影响

目的 观察成肌细胞自体移植对大鼠神经植入术后终板再生及神经肌肉功能恢复的影响,为成肌细胞在神经再生领域的应用提供实验依据,并为进一步将其作为转基因载体研究打下基础. 方法 SD雄性大鼠20只按随机数字表法分为实验组和对照组,每组10只.建立大鼠腓肠肌神经植入术模型,实验组将原代培养的成肌细胞注射到神经植入术局部,对照组只注射同体积不含成肌细胞的培养基,通过胫神经功能指数(tibial functional index,TFI)测定、神经电生理检测、病理组织学检测,观察成肌细胞对神经植入术后神经肌肉功能恢复的影响. 结果 实验组胫神经功能恢复明显快于对照组(P＜0.01);实验组大鼠腓肠肌神经电生理峰峰值(peak to peak value,PPV)、曲线下面积(area under the curve,AUC)、再生终板数量较对照组差异有统计学意义(P＜0.01). 结论 成肌细胞自体移植能使大鼠神经植入术后的神经肌肉功能恢复明显加快,再生终板数量增加.     

携带blaNDM-1耐药菌的研究进展

产生新德里金属β-内酰胺酶-1(New Delhi metallo-β-lactamase-1,NDM-1)的耐药菌携带blaNDM-1基因,能够水解除氨曲南以外几乎所有的β-内酰胺类抗生素,导致其广泛耐药,给临床治疗带来很大困难。该文就携带blaNDM-1耐药菌的发现、流行情况、分子生物学特点、检测方法等方面进行简要综述。     

HIV／AIDS患者血清自身抗体检测结果分析

目的：了解 HIV /AIDS 患者血清中自身抗体的检出率,探讨自身免疫在 HIV /AIDS 患者中的意义。方法选择2008年1月至2012年10月云南省传染病专科医院门诊和住院就诊,并检测血清抗核抗体（ANA）和抗可提取性核抗原（ENA）等15种抗体的 HIV /AIDS 患者198例为观察组,另选择2012年健康体检者50例为健康对照组。采用间接免疫荧光法检测 HIV/AIDS 患者血清 ANA ;欧蒙印迹法检测自身抗体;流式细胞术检测 CD4＋T 淋巴细胞。比较两组间自身抗体检出率。结果观察组患者自身抗体总阳性率为27．8％,以 Ro-52阳性检出率最高,ANA 和 Ro-52与健康对照组比较差异有统计学意义（P ＜0．05）。 CD4＋ T 淋巴细胞低于200个/μL 的 HIV患者自身抗体检出率为37．1％,与高于200个/μL 的 HIV 患者比较差异有统计学意义（P＜0．01）。结论 HIV 感染能诱导自身免疫反应,使患者出现多种自身抗体,自身抗体的检出率与 CD4＋ T 淋巴细胞密切相关,监测 HIV 患者自身抗体对患者的诊断和治疗有一定的指导意义。     

静脉输液组间冲管液量数学模型及体外实验研究

目的:探讨静脉输液组间冲管液量的数学模型及主要影响因素。方法:推演冲管液量的数学模型并以体外实验进行验证,分别将1mL、3mL、5mL的B液注入输液管墨菲氏小壶内,然后以A液滴注冲洗,用分光光度计测定滴出液体的吸光度,当吸光度值趋于0时收集滴出的液体量,即为实际冲管液量,并与数学模型计算所得值进行比较。结果:墨菲氏小壶内B液量分别为3mL、5mL时,实际冲管液量与计算结果比较其差异均无统计学意义(P0.05)。结论:静脉输液组间冲管液量数学模型经体外实验验证,能够指导临床工作;冲管液量主要与墨菲氏小壶内的原药液体积有关,其体积越小,所需冲管液量越少。     

SPECT固有计数率、固有均匀性质量控制检测

目的：检测单光子发射计算机断层成像系统（SPECT）的性能指标,评价其是否符合相关标准,确保临床诊断的准确性。方法用RGRMS-2012型SPECT性能模体对SPECT的固有计数率、固有均匀性进行检测,依据相关标准对检测结果进行判断,对不符合项进行修正,确保诊断的准确性。结果该SPECT的固有计数率、固有均匀性符合相关标准。结论设备在日常使用中的磨损、老化情况会对设备性能产生影响,建议定期进行设备状态检测,保证设备的可靠运行。     

经纤维支气管镜行肺泡灌洗辅助治疗支气管扩张的护理

对65例支气管扩张患者在急性感染与咯血控制后经纤维支气管镜行肺泡灌洗治疗．随访2～5年。结果显效42例，有效16例，无效7例。提示经纤维支气管镜行肺泡灌洗辅助治疗支气管扩张疗效显著，安全，患者易接受；做好常规准备工作，密切配合医生进行肺泡灌洗．严密观察病情变化，加强体位护理、饮食护理及健康教育，可保证治疗效果。     

Consideration of Helicobacter pylori infection in childhood: Immune response,endoscopic and morphological findings

Seventy-five children (aged 9–14 years) infected with Helicobacter pylori were studied endoscopically and morphologically for the signs of infection and immune response by ELISA technique (total IgE and specific IgG against H. pylori); a control group of 36 children (not infected with H. pylori) were studied simultaneously. Helicobacter pylori positive children examined endoscopically revealed a number of mucous membrane changes including erythema, erosions, lymphoid nodular hyperplasia and ulcers. Gastritis was confirmed by histology in 58 children; 6% were termed ‘active’, others were ‘non-active’. When studying the concentrations of anti-H. pylori IgG in children from the control group they were considered to be seronegative but in children infected with H. pylori a considerable increase was noted. An evaluation of the interaction between anti-H. pylori IgG titers and age, endoscopic signs and histology was carried out. Suppositions were made about the presence of links between these characteristics. Children with H. pylori infection showed a considerable increase of total IgE titers in comparison with the control group. The role of IgG and IgE in the development of chronic gastroduodenal diseases associated with H. pylori is discussed.     

Unusual suppurative complications of brucellosis in children

Three cases with suppurative complications of Brucella melitensis infection are presented, demonstrating localized involvement of the brain, bones and lungs, respectivcly, and the lesions were well defined on radiographic examination. The diagnosis was made on the basis of significant Brucella titres and positive blood culture. Awarness of such complications and performance of the appropriate serological and bacteriological studies will establish the diagnosis and will also differentiate this discase from other infections, especially tuberculosis.     

Stability of 11S globulin from Vicia faba seeds

Heat denaturation of 11S globulin, a dodecameric globular protein isolated from Vicia faba seeds was studied using scanning microcalorimetry at pH 7.6 and NaCl concentrations from 0 to 1 M. The specific enthalpy of denaturation was shown to be a linear function of temperature. The ratio of the calorimetric enthalpy to the effective one (Van't-Hoff's) per protomer of 11 S globulin was 0.9 ± 0.06. It is concluded that at first approximation 11 S globulin protomers denaturated independently in conformity with the two-state model. The plotted temperature-dependent specific free energy of 11 S globulin denaturation at different NaCl concentrations demonstrated that an increase in the salt content brought about the rise in protein stability. The maximum 11 S globulin stability is reached at about 300°K. The molar free energy of denaturation at 300°K in 1 M NaCl is 918 kJ/mol.     

EVALUATION OF MUSCULAR RELAXATION IN CONTROLLED RESPIRATION

A study of relaxation was made in 275 patients undergoing cardiacsurgery (mainly mitral valvotomy) under light narcosis withcontrolled ventilation. Suxamethonium was used for intubationand was followed by tubocurarine or gallamine for the operation.On the basis of data obtained from stimulation of the motornerves to muscles, electromyography, and the clinical picture,it is suggested that it is possible to subdivide the processof relaxation into stages. These stages would facilitate thecontrol of relaxation during surgery and in the immediate postoperativeperiod. ^* This paper was read at the All-Union Symposium on "The Useof Relaxants" on January 25, 1963.