自体骨髓基质细胞回输促进急性白血病病人化疗后骨髓造血恢复的研究

目的通过回输成纤维集落生成单位（CFU—F）促进骨髓实质细胞造血恢复。减轻白血病化疗的副作用，促进骨髓损伤早日恢复。方法选取经明确诊断的AML患者化疗回输组前后行自身对照，化疗回输前及后采骨髓行红系造血祖细胞（BFU-E）、粒、巨系集落生成单位（CFU—GM）培养，观察化疗回输前后的外周血象。结果化疗回输CFU-F后第3周。其BFU—E、CFU-GM数明显高于化疗回输前的水平（P〈0．05）。同时高于单纯化疗组第3周测得的BFU-E和CFU-GM数（P〈0．05）。结论体外扩增造血基质细胞于化疗自体回输方法，促进了病人造血恢复，对预防和减轻化疗药物造成的骨髓损伤，缩短化疗间期，加大化疗药物剂量，具有理论意义和应用价值。     

贝氏柯克斯体的分子生物学进展

Q热是由贝氏柯克斯体引起的呈世界性分布的一种人兽共患疾病。目前,世界反恐组织已将该病病原体列为生物战剂之一。早期应用抗生素对Q热有疗效,而慢性Q热治疗周期长、易复发,死亡率高。贝氏柯克斯体能以气溶胶的形式传播,一旦流行将难以控制,因此对人类危害极大。为进一步研究该病的致病机制、及时有效的诊断手段及其疫苗的开发提供参考依据,本文就贝氏柯克斯体的分子生物学研究进展作简要概述。     

武汉市室内装修后甲醛浓度动态变化规律研究

目的 了解武汉市新装修居室空气中甲醛的污染情况及其动态变化规律和室内装修装饰材料的构成.方法 于2005年4月,选择武汉市新装修的66户住户,连续6个月监测室内空气中的甲醛浓度,同时对室内装饰材料的构成做相关调查.结果 武汉市新装修居室空气中甲醛浓度在第4次监测(7月)时达到峰值,其平均浓度为0.272 mg/m,超过国家卫生标准(0.1 mg/m3)1.72倍,此后甲醛浓度随时间的延长而逐渐降低.6个月后,仍有近30%的住户室内空气甲醛浓度超标.人造板材占家具和门窗、墙面等装修材料构成的81.48%,成为室内空气甲醛污染的主要来源.结论 新装修居室空气中甲醛浓度的降低是一个长期缓慢的过程.大量使用易挥发甲醛的装饰材料(人造板材)造成新装修居室空气中甲醛污染严重.     

光子祛斑嫩肤后局部外用湿润烧伤膏的疗效观察

目的　探讨湿润烧伤膏(MEBO) 在光子祛斑嫩肤后局部外用对皮肤恢复状况的疗效。方法　将60 例激光祛斑术后的患者, 随机分为治疗组和对照组, 各30 例, 治疗组创面涂抹MEBO, 每6 h 一次, 共应用1 d ~2 d; 对照组创面仅在治疗后冷敷0． 5 h ~1 h。观察两组在术后的止痛效果、创面愈合时间、色素沉着及其消退等方面的疗效。结果　治疗组创面愈合时间、术后疼痛程度与对照组比较有显著统计学差异(P < 0． 01),色素沉着与对照组比较有统计学差异(P <0． 05)。结论　湿润烧伤膏治疗光子祛斑嫩肤术后创面疗效显著, 使用方便, 可促进创面愈合, 无明显副作用。     

267例细菌性肝脓肿患者的临床表现及病原学分析

目的分析细菌性肝脓肿患者的临床表现、病原学分布及其耐药性。方法回顾性分析267例细菌性肝脓肿患者的临床表现、病原学分布和耐药性。结果267例细菌性肝脓肿患者的主要临床表现为发热(83.89%)、畏寒或寒战(59.18%)、右上腹痛(58.05%)。外周血液白细胞增多占58.80%,门冬氨酸氨基转移酶升高占60.04%,碱性磷酸酶升高占35.96%。所获116株细菌中肺炎克雷伯菌、大肠埃希菌和金黄色葡萄球菌分别占31.03%、27.59%和8.62%。阿米卡星、头孢菌素类和泰能对革兰阴性菌敏感性较好,喹诺酮类和万古霉素对革兰阳性菌敏感性较好。结论肺炎克雷伯菌、大肠埃希菌、金黄色葡萄球菌为细菌性肝脓肿的优势菌,对当前常用抗生素敏感性较好。     

Immunization with a polyprotein vaccine consisting of the T-Cell antigens thiol-specific antioxidant, Leishmania major stress-inducible protein 1, and Leishmania elongation initiation factor protects against leishmaniasis

Development of an effective vaccine against Leishmania infection is a priority of tropical disease research. We have recently demonstrated protection against Leishmania major in the murine and nonhuman primate models with individual or combinations of purified leishmanial recombinant antigens delivered as plasmid DNA constructs or formulated with recombinant interleukin-12 (IL-12) as adjuvant. In the present study, we immunized BALB/c mice with a recombinant polyprotein comprising a tandem fusion of the leishmanial antigens thiol-specific antioxidant, L. major stress-inducible protein 1 (LmSTI1), and Leishmania elongation initiation factor (LeIF) delivered with adjuvants suitable for human use. Aspects of the safety, immunogenicity, and vaccine efficacy of formulations with each individual component, as well as the polyprotein referred to as Leish-111f, were assessed by using the L. major challenge model with BALB/c mice. No adverse reactions were observed when three subcutaneous injections of the Leish-111f polyprotein formulated with either MPL-squalene (SE) or Ribi 529-SE were given to BALB/c mice. A predominant Th1 immune response characterized by in vitro lymphocyte proliferation, gamma interferon production, and immunoglobulin G2A antibodies was observed with little, if any, IL-4. Moreover, Leish-111f formulated with MPL-SE conferred immunity to leishmaniasis for at least 3 months. These data demonstrate success at designing and developing a prophylactic leishmaniasis vaccine that proved effective in a preclinical model using multiple leishmanial antigens produced as a single protein delivered with a powerful Th1 adjuvant suitable for human use.     

地震灾害后的健康教育方式和效果初探

目的：灾后防大疫,健康教育是关键,本研究旨在了解汶川县映秀镇地震灾害发生后,开展健康教育的主要目的、方式和效果,为自然灾害后开展健康教育提供参考。方法：通过查阅资料与问卷调查,了解汶川县映秀镇震后开展健康教育的情况、效果以及存在的问题,其中效果主要通过地震前后传染病和食品卫生状况对比以及卫生知识知晓和卫生行为形成情况改变来反映。结果：震后映秀镇传染病和食品卫生状况与震前相比并没有恶化;而卫生知识知晓率与卫生行为形成率与震前相比都有较大增长。结论：灾后健康教育的主要目的是防止食源性疾病、传染病爆发等公共卫生事件;主要方式是利用镇、村两级网络,初期以面对面宣传为主要方式,条件许可后利用各种更生动的形式,灾后健康教育对促进群众卫生行为形成的实际效果十分明显,对灾后防大疫起到了重要作用。     

云南省不明原因发热患者恙虫病东方体实验室检测分析

目的 对云南省不明原因发热患者进行恙虫病东方体检测与分析。方法 应用间接免疫荧光方法（IFA）和PCR（nPCR）法对不明原因发热患者分别进行恙虫病东方体（Ot） IgG抗体和sta56基因检测及其基因序列测定分析。结果 在79例发热患者中, Ot IgG抗体阳性率48.10%, Ot核酸阳性率3.80%。基因序列分析显示序列12-17与日本O3株同源性最高为99.8%,序列12-9与中国台湾KM05、TW45R等株的同源性最高均为99.8%,序列11-6与中国台湾KM05、TW45R等株的同源性最高均为100%。进化树分析显示序列12-17、12-9和11-6均为Karp型Ot。结论 云南省恙虫病患者中存在Karp型Ot感染,其基因进化来源可能复杂。     

Effect of Gardenia extract ZG on the adsorption quantity of herpes simplex virus type 1 （HSV-1）

To observe the effect of Gardenia extract ZG on the adsorption quantity of herpes simplex virus type 1 (HSV-1) so as to explore the mechanism of its antiviral activity, fluorescein isothiocyanate (FITC) was used as the fluorescent probe to label viruses and heparin sodium was used as control. Meanwhile , the effect of Gardenia extract ZG on the adsorption quantity on the surface of Hep-2 cells was determined by flow cytometry. It was demonstrated that adsorption of HSV-1 on the surface of Hep-2 cells exhibited the character of saturation and specificity and heparin sodium could prevent attachment of viruses on these cells. These results are in accord with those reported previously. It was also proved that the manner of drug-use prior to adsorption or simultaneous use of drug and adsorption was better than adsorption prior to drug-use, and the inhibition rates of the former and latter manner were 84. 76% and 82.92% respectively. Three manners of drug-use with Gardenia extract ZG were all effective to reduce the adsorption quantity of viruses, especially the manner of simultaneous use of drug and adsorption with an adsorption inhibition rate of 68.46% . From the above observation, it is apparent that the mechanism of anti-viral activity of Gardenia extract ZG may be via several steps involved in the HSV-1 adsorption.     

邻近皮瓣修复颌面部软组织缺损34例的临床应用

目的评价应用邻近皮瓣修复颌面部软组织缺损的临床效果。方法对34例颌面部软组织缺损创面术后分别采用推进皮瓣、旋转皮瓣等多种方法转移修复。结果34例患者术后创面均I期修复,术后外形满意。结论邻近皮瓣设计灵活,采用适当的方法修复颌面部软组织缺损可以取得良好效果。