全文获取类型
收费全文 | 167篇 |
免费 | 9篇 |
国内免费 | 10篇 |
专业分类
耳鼻咽喉 | 2篇 |
儿科学 | 17篇 |
妇产科学 | 3篇 |
基础医学 | 21篇 |
口腔科学 | 4篇 |
临床医学 | 23篇 |
内科学 | 31篇 |
皮肤病学 | 2篇 |
神经病学 | 1篇 |
特种医学 | 32篇 |
外科学 | 6篇 |
综合类 | 5篇 |
一般理论 | 1篇 |
预防医学 | 8篇 |
眼科学 | 1篇 |
药学 | 18篇 |
中国医学 | 1篇 |
肿瘤学 | 10篇 |
出版年
2021年 | 1篇 |
2019年 | 2篇 |
2018年 | 2篇 |
2017年 | 2篇 |
2016年 | 3篇 |
2014年 | 2篇 |
2013年 | 5篇 |
2012年 | 3篇 |
2011年 | 3篇 |
2010年 | 5篇 |
2009年 | 9篇 |
2008年 | 4篇 |
2007年 | 10篇 |
2006年 | 4篇 |
2005年 | 5篇 |
2004年 | 5篇 |
2003年 | 1篇 |
2002年 | 8篇 |
2001年 | 5篇 |
2000年 | 10篇 |
1999年 | 3篇 |
1998年 | 12篇 |
1997年 | 8篇 |
1996年 | 5篇 |
1995年 | 6篇 |
1994年 | 10篇 |
1993年 | 10篇 |
1992年 | 6篇 |
1991年 | 1篇 |
1990年 | 2篇 |
1989年 | 7篇 |
1988年 | 2篇 |
1987年 | 3篇 |
1986年 | 4篇 |
1985年 | 1篇 |
1984年 | 2篇 |
1983年 | 2篇 |
1980年 | 3篇 |
1979年 | 1篇 |
1977年 | 1篇 |
1976年 | 5篇 |
1975年 | 2篇 |
1875年 | 1篇 |
排序方式: 共有186条查询结果,搜索用时 15 毫秒
131.
Ciria HC Quevedo MS Cabrales LB Bruzón RP Salas MF Pena OG González TR López DS Flores JM 《BMC cancer》2004,4(1):1-10
Background
Tumor-associated antigens recognized by humoral effectors of the immune system are a very attractive target for human cancer diagnostics and therapy. Recent advances in molecular techniques have led to molecular definition of immunogenic tumor proteins based on their reactivity with autologous patient sera (SEREX).Methods
Several high complexity phage-displayed cDNA libraries from breast carcinomas, human testis and breast carcinoma cell lines MCF-7, MDA-MB-468 were constructed. The cDNAs were expressed in the libraries as fusion to bacteriophage lambda protein D. Lambda-displayed libraries were efficiently screened with sera from patients with breast cancer.Results
A panel of 21 clones representing 18 different antigens, including eight proteins of unknown function, was identified. Three of these antigens (T7-1, T11-3 and T11-9) were found to be overexpressed in tumors as compared to normal breast. A serological analysis of the 21 different antigens revealed a strong cancer-related profile for at least five clones (T6-2, T6-7, T7-1, T9-21 and T9-27).Conclusions
Preliminary results indicate that patient serum reactivity against five of the antigens is associated with tumor disease. The novel T7-1 antigen, which is overexpressed in breast tumors and recognized specifically by breast cancer patient sera, is potentially useful in cancer diagnosis. 相似文献132.
Rasha?DabashEmail author Jyoti?Vajpayee Martha?Jacob Ilana?Dzuba Nisha?Lal Jan?Bradley LB?Prasad 《Reproductive health》2005,2(1):11
Background
Despite being a preventable disease, cervical cancer claims the lives of almost half a million women worldwide each year. India bears one-fifth of the global burden of the disease, with approximately 130,000 new cases a year. In an effort to assess the need and potential for improving the quality of cervical cancer prevention and treatment services in Uttar Pradesh, a strategic assessment was conducted in three of the state's districts: Agra, Lucknow, and Saharanpur. 相似文献133.
Central nervous system disease in acquired immunodeficiency syndrome: prospective correlation using CT, MR imaging, and pathologic studies 总被引:2,自引:0,他引:2
Post MJ; Sheldon JJ; Hensley GT; Soila K; Tobias JA; Chan JC; Quencer RM; Moskowitz LB 《Radiology》1986,158(1):141-148
A prospective study compared the abilities of high-resolution computed tomography (HRCT) and magnetic resonance (MR) imaging in detection and evaluation of central nervous system disease in neurologically symptomatic patients with acquired immunodeficiency syndrome (AIDS). Eighteen CT scans and 19 MR images in 14 patients were compared. HRCT images with contrast material enhancement were superior to unenhanced 0.35-T MR images for differentiating a lesion from surrounding edema, discriminating between lesions in close proximity, locating lesions for biopsy, judging lesion activity, detecting small cortical lesions with minimal edema, and spatial resolution. MR imaging was superior to CT scanning in evaluation of white-matter lesions and detection of small lesions surrounded by edema. MR imaging exhibited higher contrast resolution and greater sensitivity. Complementary uses of MR and CT imaging are suggested. 相似文献
134.
135.
136.
Thoracic duct cyst: diagnosis with needle aspiration 总被引:3,自引:0,他引:3
A case of an asymptomatic posterior mediastinal thoracic duct cyst diagnosed with fluoroscopically guided needle aspiration is reported. Previously, the diagnosis of thoracic duct cyst was established surgically and more recently was established by lymphangiography. To the authors' knowledge, diagnosis using needle aspiration has not been reported. 相似文献
137.
138.
Fay LB; Leaf CD; Gremaud E; Aeschlimann JM; Steen C; Shuker DE; Turesky RJ 《Carcinogenesis》1997,18(5):1039-1044
The urinary excretion of the DNA alkylation product, 3-methyladenine (3-
MeAde), was measured in human volunteers who were on controlled diets and
consumed fresh fish, or frozen-stored fish that contained 50-fold higher
levels of dimethylamine (DMA), with or without ingested nitrate. DMA
potentially could react with nitrosating agents in the diet or within the
body, and produce the potent carcinogen N- nitrosodimethylamine (NDMA),
which can then react with DNA to form several adducts including 3-MeAde.
Our findings show that there was no increase in urinary levels of 3-MeAde
after consumption of fish preserved by frozen storage relative to levels
after consumption of fresh fish. Furthermore, consumption of 225 mg sodium
nitrate (equal to the nitrate content in a large glass of beet juice) at 1
h prior to consumption of the frozen-stored fish did not increase urinary
3-MeAde levels as would be expected if nitrate enhanced endogenous
nitrosation of DMA. In contrast, urinary excretion of 3-MeAde from a
volunteer who was a moderate cigarette smoker (11 cigarettes per day) was
approximately 3- to 8-fold higher than dietary 3-MeAde intake. These
findings indicate that consumption of high levels of DMA in fish does not
result in detectable levels of NDMA formation and genetic damage as
measured by the urinary biomarker 3-MeAde.
相似文献
139.
JS Bradley SL Kaplan TQ Tan WJ Barson M Arditi GE Schutze ER Wald LB Givner EO Mason 《Pediatrics》1998,102(6):1376-1382
OBJECTIVE: To describe the clinical and microbiological characteristics of infants and children with bone and joint infections caused by penicillin-susceptible and penicillin-nonsusceptible strains of Streptococcus pneumoniae. DESIGN: Multicenter, prospective patient accrual; retrospective chart review of identified patients. SETTING: Eight children's hospitals in the United States. PARTICIPANTS: Forty-two children with bone and/or joint infections prospectively enrolled in the United States Pediatric Multicenter Pneumococcal Surveillance Study from September 1, 1993 to August 31, 1996. OUTCOME MEASURES: Data were collected on multiple variables, including age, gender, race, days of symptoms before and during hospitalization, antibiotic and surgical therapy, laboratory and imaging studies. RESULTS: Of the 42 children enrolled (21 bone, 21 joint infections), 14 had isolates that were not susceptible to penicillin. Eight of 16 (50%) strains isolated from children who received antibiotics within 4 weeks before hospitalization were not susceptible to penicillin, compared with 4 of 15 (27%) strains isolated from children without previous antibiotic exposure. Clinical response to therapy was similar between children infected by penicillin-susceptible strains compared with those infected by penicillin-nonsusceptible strains, including duration of hospitalization (9.1 days vs 11.2 days), days of intravenous antibiotic therapy (25.3 days vs 24.6 days), days of fever (3.6 days vs 3.1 days), and sequelae (14% vs 7%). The most commonly prescribed single agents for parenteral therapy in definitive treatment were ceftriaxone (36%), penicillin (15%), and clindamycin (15%). Oral therapy followed parenteral therapy in 56% of children. The mean (+/- standard deviation) duration of total antibiotic therapy in children with osteomyelitis was 57.5 +/- 48.6 days (range, 23-196 days) and 29.2 +/- 11.8 days (range, 12-67 days) for arthritis. Late sequelae (long-term destructive changes of the bone or joint) were documented in 5 (12%) children, 4 with osteomyelitis, and 1 with arthritis. Sequelae occurred in 30% of children with long bone osteomyelitis associated with infection in the adjacent joint. The age of children with sequelae was younger than those without sequelae (6.4 months vs 18.6 months). CONCLUSIONS: The demographic characteristics and anatomic sites of infection in our patients were similar to previously published series collected from single institutions before the emergence of significant antibiotic resistance in S pneumoniae. Our analysis suggests that children infected by penicillin-nonsusceptible strains have a similar clinical response to therapy when compared with children infected by penicillin-susceptible strains. 相似文献
140.
Differential expression pattern of XqPAR-linked genes SYBL1 and IL9R correlates with the structure and evolution of the region 总被引:3,自引:1,他引:3