Correlation between proliferation and apoptosis in different histological types of uterine leiomyoma

75 cases of simple, cellular and mitotically active leiomyoma (LM) were studied immunohistochemically with the use of monoclonal antibodies against bcl-2 ("DAKO", 1:80), bax ("Calbiochem, 1:80), c-myc ("Novocastra", 1:100), CD 95 ("Dianova, 1:50), Ki67 ("Dianova", 1:40), PCNA ("Novacastra", 1:100). Apoptosis was assessed by the Tunnel method (ENZO Apop Detek Cell Death Assay System). Simple LM is characterized by apoptosis domination over proliferation in leiomyocytes. Size increase of such a tumor is probably the result of hypertrophy of leiomyocytes and stromal elements proliferation as well as secondary changes. Cellular LM is characterized by about similar level of tumor cell proliferation and apoptosis. Size increase of cellular LM is related to hypertrophy and leiomyocytes number increase probably due to their life lengthening. Mitotically active LM is characterized by domination of proliferation over apoptosis of tumor cells this being main condition of this tumor growth. Various types of LM differ by their stroma composition: fibrocyte prevailing in a simple LM, angiogenesis in mitotically active LM. Combination of adenomyosis with endometrium hyperplasia results in enhancement of proliferation and apoptosis of tumor cells.     

Morpho- and angiogenetic characteristics of uterine leiomyoma

Ordinary and cellular leiomyomas of 147 patients (age 23 - 65 years) were studied histologically and immunomorphologically. Five females with rapidly growing tumors were operated after therapy with gonadotropines blockers. Variants with weak and pronounced proliferative activity of neoplastic myocytes and various activity of neoangiogenesis were distinguished. The latter may be either phases of development or different tumour types. Use of gonadotropine blockers inhibits myocyte proliferation and activates formation of stroma.     

Factors and conditions affecting the process of infecting the fetus during different pregnancy stages

Etiology, pathogenesis of intrauterine infection of the fetus, factors promoting such infection and the status of the local immune system are elucidated in the paper. A changed reactivity of the immune homeostasis of a pregnant woman with a chronic infection was shown to be potentially an important factor of pathogenesis of defects in her baby. The local immunity factors of the reproductive tract, i.e. the vagina and neck of uterus, are in the forefront of anti-infectious protection, which signal an alert or limit the microorganisms' reproduction; besides, they prevent the microorganisms from penetrating into the upper genitals. The therapeutic-and-preventive schemes applicable to infected women must comprise measures targeted at eliminating an infectious agent and at normalizing the pathological changes in the performance of the immune system activity.     

Clinical value of the antioxidative activity of blood serum and lacrimal fluid in endogenous uveitis in children

The method of chemiluminescence in the system of hemoglobin-peroxide hydrogen-luminal was made use of to study the antioxidant activity (AOA) of lacrimal fluid and blood serum in 20 children with endogenous uveitis. As uveitis aggravated, there is observed a reduced AOA both in lacrimal fluid and blood serum. As the inflammatory process is arrested, AOA begins to go down gradually; moreover, it approaches the values in the control group. The correlation between the lacrimal fluid AOA and the blood serum AOA was calculated and analyzed for each eye for the moment of examination. The low values of the coefficient were found for the constantly relapsing uveitis, while the high values were typical for the initial remission phase. A study of the correlation between the blood serum AOA and the lacrimal fluid AOA showed a low correlation coefficient (r = 0.3) during the uveitis aggravation period and its increase during the remission period (r = 0.7). The obtained data are indicative of that the free-radical processes are involved in the pathogenesis of endogenous uveitis; they also show that it is advisable to study the lacrimal-fluid and blood-serum AOAs in order to forecast the above disease and to choose an optimal therapy methods.     

Comparison of urethral and rectal methods of treatment of chronic bacterial urethroprostatitis with application of the AMUS-01-Intramag

Our study has shown that the same set of physical factors in combination with local drug therapy when used endourethrally has a higher effect in therapy of chronic bacterial urethroprostatitis (CBUP). Mean subjective indices (pain, dysuria, questionnaire data) in the urethral technique are 1.33 times higher than those in the rectal technique. The objective indices (leukocytes, licetine granulations) - 1.4, uroflowmetry parameters - 1.51, hemodynamic parameters - 1.4 times higher. The complex AMUS-01-Intramag has some advantages over the other complexes, the main advantage being the ability to provide local drug therapy and accumulation of the drug in the affected focus.     

Risk of intrauterine infection of the human fetus with Coxsackie viruses in pregnancy complicated by late toxicosis and pyelonephritis

During virological examinations of 144 pregnant women including 85 women with complicated course of pregnancy Coxsackie A viruses were isolated from the blood or identified in the cells of urinary tracts in 60.3% of the cases of nephropathy, in 68.3% of the pregnant women with pyelonephritis, and in 8.5% of normal subjects. The groups of the pregnant women under comparison did not differ significantly in the rate of finding of other viruses. Coxsackie A viruses were also isolated from the placenta and amniotic fluid in 3 out of 4 cases examined in late toxicosis, were found in the kidneys of the fetus from a woman with pyelonephritis and in cells of the urinary tracts of 13 out of 19 babies (68.4%) born to women with complicated course of pregnancy. The hypothesis of the etiological association of nephropathy and pyelonephritis in pregnant women with activation of chronic Coxsackie virus infection and of the high risk of infection of the fetus with these viruses in the described complications of pregnancy is discussed.     

Changes in regenerated and normal liver in rats during the lactation period

Summary In female rats weighing 160–200 g two-thirds of the liver were excised after Higgins' and Andersen's technique. A month after the operation, upon completion of the regeneration process, a study was made of the influence of functional load (pregnancy and lactation) on the regenerated and normal liver in the rats.(Presented by Academician N. A. Kraevskii) Translated from Byulleten' Éksperimental'noi Bioligii i Meditsiny, Vol. 59, No. 5, pp. 93–96, May, 1965