OCTA在地塞米松玻璃体内植入剂治疗RVO-ME短期疗效观察中的应用

目的：以地塞米松玻璃体内植入剂(IDI)玻璃体腔注射治疗视网膜静脉阻塞继发黄斑水肿(RVO-ME),利用光相干断层扫描血管成像技术(OCTA)等检查评价其短期疗效及安全性。

方法：对确诊为RVO-ME患者17例17眼进行玻璃体腔注射IDI治疗,分别于治疗前,治疗后1d,1、2、3、4、6mo等时间点测定患者最佳矫正视力(BCVA)、眼内压(IOP)、黄斑中心凹厚度(CMT),观察浅、深层视网膜血管网变化。

结果：患者17眼经IDI治疗后24h患者CMT明显下降,且随着时间的推移,CMT整体亦呈下降趋势,在3mo随访时,CMT改善较为明显; 视力改善与CMT变化一致; OCTA显示视网膜血管网较基线期恢复。IDI注射治疗后共有2眼出现轻度眼压增高,用药后均可恢复至正常。随访期间未见与注射及药物治疗相关不适及副作用。

结论：玻璃体腔注射IDI治疗视网膜静脉阻塞继发黄斑水肿的短期疗效确切,对患者视力提升明显,且安全性较高,未见明显不良反应发生。OCTA可以观察到黄斑水肿明显减轻,浅、深层视网膜血管网血管迂曲和扩张显著改善。     

The Effect of Different Treatments of (–)-Epigallocatechin-3-Gallate on Colorectal Carcinoma Cell Lines

Backgroud: (-)-Epigallocatechin-3-gallate (EGCG), the major component of green tea, is well documented to induce apoptosis and cell cycle arrest in cancer by targeting multiple signal transduction pathways. However, EGCG is extremely unstable in general culture conditions and rapidly degraded. So, to what extent EGCG or which degradation products of EGCG play a role in anti-tumor is still unknown. In this study, we evaluated the effect of different treatments of EGCG on HCT116 cells.

Design: MTT assay was applied to evaluated the inhibitory effect of different treatments of EGCG on HCT116 cells. Cell cycle and apoptosis were performed by flow cytometry. Finally, western blot analysis was used to elucidate the molecular mechanism associated with cell cycle arrest and apoptosis.

Results: Compared with control, both EGCG and O-EGCG (i.e., EGCG being pre-incubated at 37°C for 3 h) significantly inhibited HCT116 cells growth. Surprisingly, we found that the inhibitory effect of O-EGCG was stronger than that of EGCG. The IC₅₀ values of EGCG and O-EGCG were 8.75 and 5.40 μM, respectively. Cell cycle analysis showed that 20 μM of EGCG simultaneously caused cell cycle arrest at G1 and G2 phase in HCT116 cells, differing from O-EGCG which exclusively caused cell cycle arrest at G2. This result suggested that parent EGCG at the early treatment might cause cell cycle arrest at G1. As time went on, EGCG disappeared and degraded products of EGCG were formed which might cause cell cycle arrest at G2. Further studies revealed that EGCG induced cell cycle arrest at G1 by downregulation of cyclin E and cyclin D1 and upregulation of p21. On the other hand, O-EGCG induced HCT116 cells apoptosis mainly by increasing the expression of p53 and cleaved caspase-3, which might be the underlying reason why O-EGCG had stronger inhibitory effect on HCT116 cells line than EGCG.

Conclusions: The pretreatment of EGCG may be an effective way to enhance its antitumor effect.     

益气活血方促心梗后大鼠缺血心肌血管新生的实验研究

目的：观察益气活血方对心梗后大鼠缺血心肌局部血管新生的影响。方法：建立心梗模型后，分为五组，即益气活血方大剂量组、益气活血方小剂量组、麝香保心丸组、假手术组、模型组，灌胃6周后处死动物，取心肌组织，用免疫组化法（CD34）测定心梗边缘区新生血管数及微血管密度。结果：与假手术组相比，模型组大鼠心梗边缘区新生血管数及微血管密度明显升高（P〈0.05）；所有治疗组对新生血管数及微血管密度均有不同程度的促进作用，与模型组比较均有显著差异（P〈0．05），其中大剂量组和麝香保心丸组的作用尤为明显（P〈0.05）。结论：益气活血方能够促进心梗后大鼠缺血心肌局部血管新生，对缺血心肌具有保护作用。     

中国四大名菊的产地适宜性研究

目的:明确四大名菊的重要生态因子,分析其在中国的适宜生长区域,为四大名菊的引种扩种以及合理规划生产布局提供参考;方法:本文综合实地采样、标本查阅和文献研究结果,应用ArcGIS和气候数据库提取各样点生态因子,得出四大名菊适宜的生态因子范围,并以此为依据应用中药材产地适宜性分析平台(TCMGIS-Ⅱ)对四大名菊的生态适宜...     

颅内脑动脉瘤夹力学性能的研究

研究了血管组织的力学性能和颅内脑动脉瘤夹的机械力学性能，结果表明各种瘤夹的物理特性，诸如夹闭力，叶片的最大张角、宽度、厚度和材料性能对满足医学应用都是至关重要的，夹闭力的测试技术也得到了成功地解决。     

Effect of hexasulfobutylated C(60) on the isolated aortic ring of guinea pig.

The effects of hexasulfobutylated C(60) (FC(4)S) and monomalonic acid C(60) (MMA C(60)), the fullerene C(60) derivatives, on isolated endothelium-containing or endothelium-denuded aorta of guinea pig were studied pharmacologically in vitro. In the endothelium-containing preparation of the aortic rings, phenylephrine (PHE) elicited contracture and acetylcholine (ACh) elicited a relaxing effect on the PHE-precontracted preparation. In the PHE-precontracted preparation, MMA C(60) (10 micromol/l) did not elicit a relaxing effect on the PHE-precontracted preparation. However, MMA C(60) (10 micromol/l) significantly reduced the maximum response of the relaxation elicited by ACh. FC(4)S itself significantly relaxed the PHE-precontracted aortic rings. ACh-induced relaxation in PHE-precontracted endothelium-containing strips of the aortic rings was significantly potentiated if pretreated with FC(4)S (10 micromol/l). In the denuded aortic rings, FC(4)S did not elicit the relaxing effect in the PHE-precontracted preparation. The relaxing effect of FC(4)S on the PHE-precontracted preparation was not altered when superoxide dismutase (250 units/ml) was pretreated. However, the relaxing effect was reduced when N(G)-nitro-L-arginine methyl ester (L-NAME, 1 mmol/l) or methylene blue (1 micromol/l) was pretreated. These results demonstrated that the vasorelaxation effect of FC(4)S on the PHE-precontracted aortic ring is partly dependent on the release of nitric oxide (NO) or an NO-derived substance from the vascular endothelium.     

工频磁场对星形胶质细胞间隙连接通讯功能的影响

目的　探讨工频磁场 (PFMF)是否有促癌或协同促癌作用。方法　利用荧光光漂白后再恢复法观察漂白细胞荧光强度的恢复以判断经间隙连接的细胞间通讯 ,以相对荧光强度恢复速率(CFIRR)作为对细胞间隙连接通讯 (GJIC)作用的评价指标 ,研究不同磁场强度单独作用或协同佛波酯(TPA)对星形胶质细胞GJIC功能的影响。结果　 3ng/mlTPA作用 1h时CFIRR的中位数 (Md)值为4 .53 % /min ,空白对照组为 9.74% /min ,两组的差异有显著性 (H =1 2 .0 84,P <0 .0 0 5)。 0 .8或 1 .6mT磁场作用 2 4h时CFIRR的Md 分别 8.2 5、6 .68% /min ,与空白对照组比较 ,差异无显著性 (H =32 .61 7,P >0 .0 5)。 0 .8或 1 .6mT磁场作用 2 3h ,再与TPA共同作用 1h时CFIRR的Md 分别为 3 .32、2 .85% /min ,与TPA组比较 ,差异无显著性 (H =2 .589,P >0 .0 5)。结论　 0～ 1 .6mT的 50Hz磁场单独作用不能抑制星形胶质细胞GJIC功能 ;协同TPA ,不能增强TPA对星形胶质细胞GJIC的抑制作用 ;但是磁场对星形胶质细胞GJIC的抑制作用随着磁场强度增强呈递增趋势     

Tuberculin reactivity in adults after 50 years of universal bacille Calmette-Guerin vaccination in Taiwan

We aimed to assess whether tuberculin reactivity in adults is affected by bacille Calmette-Guerin (BCG) vaccination after 50 years of universal BCG vaccination with 80-95% coverage. A community-based study on tuberculin reactivity in 619 participants was conducted in February 2000 in Keelung city, Taiwan. Information on BCG vaccination policies and annual risk of infection (ARI) in the underlying population was extracted from consecutive national prevalence surveys relating to the period 1952-1997. Compared with the expected ARI estimate, the standardized morbidity ratio of positive tuberculin response for vaccination in infancy was 2.2 (95% CI 0.3-15.5) for those aged <10 years. The corresponding figures for older age groups ranged from 3.6 (95% CI 2.2-5.9) for those aged 10-12 years to 0.7 (95% CI 0.5-0.9) for those aged 57-67 years. This suggests that the effect of BCG vaccination on positive tuberculin response in adults aged >30 years is probably negligible irrespective of age at vaccination or revaccination and that the tuberculin skin test can be used to diagnose TB in control programmes in countries with moderate or high incidence of TB.     

室内空气净化方法及性能评价

本文列举了常用的室内空气净化方法,重点阐述了臭氧灭菌的机理、影响紫外线杀菌效果的因素及纳米光催化在消除室内污染方面的优势.最后,介绍了室内空气净化器的性能评价标准.     

应用K-ras基因突变诊断胰腺癌的探讨

目的 采用连续富集酶切一限制性片段长度多态性／聚合酶链反应（CED-RnP／PCR）探索K-ras基因在胰腺癌患者血浆中的表达。方法 以血浆代替以往的标本并采用CED-RFLP／PCR技术检测胰腺癌、非胰腺癌患者及健康对照者血浆K-ras基因突变情况。结果 胰腺癌患者血浆K-Fas基因的突变阳性率为73％，无假阳性率，高于胰液、十二指肠液的检测率，低于细针穿刺的检测率；胰腺良性病变患者及健康对照者血浆中未检测到K-ras基因的突变；胰腺癌组K-ras基因突变与非胰腺癌组及健康对照组相比具有显著性差别。结论 CED-RFLP／PCR法检测血浆K-ras基因突变技术具有简单、易行高效的特点，克服以往技术的弊端，对胰腺癌的诊断及鉴别有一定的应用价值。