二元混合表面活性剂在固液界面上的协同效应

本文介绍了以非离子(AEO_9,AEO_3 TX10)和阴离子(C_(12)H_(25)SO_8Na)表面活性剂组的二元混合物,在固液界面上存在着十分活跃的增效作用。在γIv为0.04牛/米时,C_(12)H_(25)SO_3Na—AEO_9二元混合物在Tcflon表面上的粘附自由(?),在很宽的区域内呈现稳定的增效作用。与气液界面的协同效应对比,二元混合物在固液界面上的增效作用出观更为频繁而且形式多样。这对润湿剂工业配方提供了依据。     

EphA1基因在前列腺癌中的表达及临床意义

目的 检测EphA1基因在前列腺癌的表达,探讨其异常表达机制及其临床意义.方法 利用RT-PCR检测印EphA1在前列腺癌细胞系中的表达,并用重亚硫酸钠修饰的DNA直接测序法检测并分析其甲基化状态.免疫组织化学分析EphA1蛋白、p53蛋白、EGFR蛋白在前列腺癌组织中的表达及其相关性在临床中的意义.结果 EphA1蛋白表达在前列腺癌和良性增生组织中差异有统计学意义(P=0.013),并且发现在前列腺癌细胞存在EphA1基因甲基化现象.EphA1蛋白表达上调与前列腺癌有较高的Gleason评分患者有相关性(P=0.017),并与EGFR阳性有相关性(P=0.056),EphA1蛋白下调与p53阳性有相关性(P=0.007).结论 EphA1甲基化可能是导致该基因下调的机制之一,在前列腺癌的发生、发展中可能具有重要作用.     

Auricular plaster therapy to relieve poststroke fatigue

目的 探讨耳穴贴压疗法对缓解脑卒中后患者疲劳的作用.方法 将66例脑卒中后疲劳患者随机分为对照组与观察组各33例.对照组实施常规治疗与健康教育;观察组在此基础上,取神门、皮质下、交感、缘中、心、肝、脾、肾耳穴贴压进行干预,2个疗程后评价效果.结果 观察组在干预后表现体力及疲劳总分显著低于对照组(P＜0.05,P＜0.01).结论 通过运用耳穴贴压疗法对脑卒中后疲劳患者进行干预,可以有效地缓解患者的疲劳水平,提高患者的生活质量.     

Symptom experience and quality of life in patients receiving transcatheter arterial chemoembolization

目的 了解肝癌患者行肝动脉化疗栓塞(TACE)术后的症状严重度、生活质量,分析两者的关系,以从症状干预角度改善患者术后生活质量.方法 采用Anderson症状量表,自行设计的肝癌特异性症状增补条目,以及FACT G量表于首次TACE术后第3天对142例患者进行症状和生活质量测评.结果 TACE术后患者生活质量均分为(64.16±10.87)分;Anderson症状量表症状严重度均分(3.41±1.29)分,肝癌特异性症状严重度均分(2.36±1.18)分.疼痛、悲伤感、食欲下降、腹胀、疲乏是影响惠者术后生活质量最为显著的5个因素(P＜0.05,P＜0.01).结论 TACE术后患者生活质量不高,症状是患者生活质量的重要影响因素.临床护理人员除重视较为突出的疼痛症状外,也要关注悲伤、食欲下降、腹胀和疲乏症状,探索实际有效的干预方法,提高患者生活质量.     

耳石手法复位治疗后半规管良性阵发性位置性眩晕

目的比较两种耳石手法复位治疗后半规管良性阵发性位置性眩晕(PC-BPPV)的疗效和安全性。方法 78例单侧PC-BPPV患者分为Ep ley手法治疗组和B randt手法治疗组。计算治疗后7 d、半个月的缓解率,观察治疗的不良反应。结果 Ep ley手法治疗组首次及2周的缓解率分别为61.9%、76.1%;B randt手法治疗组为66.6%、77.6%,两组比较差异无统计学意义。Ep ley手法治疗组出现不良反应7例,B randt手法治疗组出现不良反应3例。结论耳石手法复位治疗BPPV是一种非常有效的方法,B randt手法治疗操作更简单,容易被患者接受。     

Nitrite Anion Therapy Protects Against Chronic Ischemic Tissue Injury in db/db Diabetic Mice in a NO/VEGF-Dependent Manner

Nitrite anion has been demonstrated to be a prodrug of nitric oxide (NO) with positive effects on tissue ischemia/reperfusion injury, cytoprotection, and vasodilation. However, effects of nitrite anion therapy for ischemic tissue vascular remodeling during diabetes remain unknown. We examined whether sodium nitrite therapy altered ischemic revascularization in BKS-Lepr^db/db mice subjected to permanent unilateral femoral artery ligation. Sodium nitrite therapy completely restored ischemic hind limb blood flow compared with nitrate or PBS therapy. Importantly, delayed nitrite therapy 5 days after ischemia restored ischemic limb blood flow in aged diabetic mice. Restoration of blood flow was associated with increases in ischemic tissue angiogenesis activity and cell proliferation. Moreover, nitrite but not nitrate therapy significantly prevented ischemia-mediated tissue necrosis in aged mice. Nitrite therapy significantly increased ischemic tissue vascular endothelial growth factor (VEGF) protein expression that was essential for nitrite-mediated reperfusion of ischemic hind limbs. Nitrite significantly increased ischemic tissue NO bioavailability along with concomitant reduction of superoxide formation. Lastly, nitrite treatment also significantly stimulated hypoxic endothelial cell proliferation and migration in the presence of high glucose in an NO/VEGF-dependent manner. These results demonstrate that nitrite therapy effectively stimulates ischemic tissue vascular remodeling in the setting of metabolic dysfunction that may be clinically useful.     

日本血吸虫重组抗原的免疫原性鉴定

为发展新的日本血吸虫病疫苗候选抗原分子，对已构建的阳性表达克隆PGsj24进行大量诱导表达，制备为20kD的重组抗原。以之免疫家兔，产生了较强的抗体反应。该单特导抗血清可识别成上天然蛋白中与重组蛋白相对应的抗原成分。两者分子量及Westernblot识别反应带型均基本一致，说明约20kD重组蛋白确为日本血吸虫基因编码产物，具有较强的免疫原性，可刺激机体产生较强的免疫应答。     

SKLB1002, a novel inhibitor of VEGF receptor 2 signaling, induces vascular normalization to improve systemically administered chemotherapy efficacy

Vascular endothelial growth factor receptor (VEGFR) or vascular endothelial growth factor (VEGF) inhibitors have shown only modest clinical activity for most tumor types when used as single agents. However, present evidence indicates that these antiangiogenic drugs can cause transient "normalization" of the tumor vasculature, thereby improving the delivery of systemic chemotherapy. We examined temporal changes in tumor vascular function in response to the novel VEGFR2 inhibitor, SKLB1002. Established tumor-bearing animals were evaluated at serial time points for treatment-associated changes in tumor vascular architecture and function. As a result, blocking VEGF signaling by SKLB1002 produced a morphologically and functionally "normalized" vascular network. Consistent with our observations, a 2.2 fold increase in intratumoral doxorubicin levels was determined with SKLB1002 pretreatment compared with administration of doxorubicin alone. Finally, combined SKLB1002 and doxorubicin exhibited significant antitumor (49% of control size) and antimetastatic effects (12% of control metastatic nodules) in vivo. Our results showed SKLB1002 induced vascular normalization and enhanced anticancer drug delivery, which were associated with the observed synergistic effect in vivo.     

Expression and Clinical Significance of UCH37 in Human Esophageal Squamous Cell Carcinoma

Background

Ubiquitin carboxyl-terminal hydrolase 37 (UCH37), a member of the DUBs, was found to play an important role in oncogenesis through promoting some Proto-oncogenes’ expression and stem cell-like characteristics in the cell in previous research. The aim of this study was to assess the value of UCH37 in predicting tumor recurrence after curative resection in esophageal squamous cell carcinoma (ESCC) patients.

Methods

We analyzed UCH37 protein expression in 111 clinicopathologically characterized ESCC cases, from those who underwent curative resection between 2007 and 2008, by immunohistochemistry. The prognostic significance was assessed using Kaplan–Meier survival estimates and log-rank tests.

Results

We found that UCH37 expression was higher in the cancer tissue than in non-tumorous control tissue at protein level and was overexpressed in tumor tissues of recurrent patients. There was a significant difference of UCH37 expression in patients categorized according to TNM stage (p?=?0.038) and lymph nodes metastasis condition (p?=?0.009). Univariate analyses revealed that UCH37 was a significant predictor for overall survival and disease-free survival, and multivariate analyses showed that UCH37 was an independent prognostic marker for ESCC recurrence. A prognostic significance of UCH37 was also found in the subgroup of lymph nodes metastasis condition classification. About 90?% of the recurrent patients recurred within 2?years, of which 84.4?% were predicted by UCH37.

Conclusion

UCH37 is associated with outcome and recurrence of ESCC and can be a novel predictor for poor prognosis of ESCC patients after curative resection.