转录因子Tbx18在小鼠胚胎发育过程中的时空表达

目的 检测胚胎期小鼠Tbx18在mRNA及蛋白水平的表达情况,了解Tbx18在小鼠胚胎发育过程中的时空表达模式.方法 用整体原位杂交技术检测小鼠整胚TBX18 mRNA的表达,用X-gal染色检测Tbx18-Cre/Rosa26R/LacZ 双杂合基因谱系示踪模型小鼠胚胎的报告基因LacZ蛋白的表达,用荧光定量PCR检测小鼠胚胎期心脏mRNA的定量表达.结果 发现在小鼠胚胎期9.5～10.5 d转录因子Tbx18主要在前体心外膜表达,由前体心外膜逐渐迁移定位于心外膜;在11.5～12.5 d的小鼠胚胎Tbx18主要定位于心脏、上下肢、体节及上下唇鄂区域;大于12.5d的小鼠胚胎Tbx18除了上述部位外还定位于肾、输尿管、膀胱等区域.结论 转录因子Tbx18在小鼠胚胎发育过程中的表达具有时空特异性,对小鼠心脏的发育可能起着至关重要的作用;同时在上下肢、体节、唇鄂、肾、输尿管、膀胱的发育中也可能起非常重要的作用.     

伴非自杀性自伤行为的青少年心理体验的Meta整合

背景非自杀性自伤（NSSI）是世界范围内较严重的公共卫生问题,也是自杀未遂和自杀死亡的重要危险因素。我国青少年NSSI行为的发生率为27.4%,需重视青少年NSSI行为,防止其进一步发展为更严重的心理健康问题。目的 系统评价与整合伴NSSI行为的青少年的心理体验及需求,以期为临床干预方案的制定提供参考。方法 计算机检索PubMed、Web of Science、The Cochrane Library、PsycINFO、CINAHL、Embase、Medline、中国知网、万方数据库、维普数据库、中国生物医学文献数据库,收集关于伴NSSI行为的青少年心理体验及需求的质性研究,检索时限为建库至2023年3月。采用澳大利亚循证卫生保健中心（JBI）质性研究质量评价工具对纳入文献进行评价,并运用汇集性整合的方法对结果进行整合。结果 共纳入11篇文献,提取出46个研究结果,将相似结果汇总成10个类别,最终得到5个整合结果,包括人际关系冲突、学业压力与自我思想斗争、内心情感交织的自伤行为、爱能克服无助感以及调整与寻求积极应对。结论 青少年在发生NSSI行为前,往往可能经历负性生活事件,并且在人际...     

彩色多普勒超声对主动脉夹层的诊断价值（附48例分析）

目的探讨彩色多普勒超声（简称彩超）对主动脉夹层的诊断价值。方法应用彩超测量主动脉内径,观察主动脉及其分支内膜分离的部位和范围,鉴别真假腔。观察主动脉瓣返流情况,评价左室功能,并与MRI、多层螺旋CT血管成像（MSCTA）进行比较分析。结果彩超、MRI及MSCTA诊断主动脉夹层的准确率分别为65．8％,89．7％及90．0％。彩超能更好地检出主动脉瓣关闭不全、左室功能及心包积液情况。结论彩超检查能正确评价本病受累血管,是诊断主动脉夹层的一种较为可靠的方法。     

Striated intramural gallbladder lucencies on US studies: predictors of acute cholecystitis

Ultrasound scans of 51 consecutive patients with gallbladder wall thickening were reviewed, and specific sonographic features were correlated with surgical and clinical follow-up. Two patterns of thickening were identified as specific indicators of the presence or absence of acute cholecystitis. "Striated" wall thickening, consisting of several alternating, irregular, discontinuous, lucent and echogenic bands, was seen in eight of 13 patients (62%) with acute cholecystitis. This pattern was not encountered in any of the patients who did not have acute cholecystitis. Conversely, "three-layer" thickening, consisting of a single circumferential lucent zone between two relatively uniform echogenic layers, was seen in only one of 13 patients (8%) with acute cholecystitis but in 11 of 38 patients (29%) with other diagnoses. Other abnormalities, including the presence of intramural echogenic foci and wall irregularities, were more frequently seen in patients with acute cholecystitis but were not as helpful. Use of these features may suggest or help exclude a diagnosis of acute cholecystitis in those patients in whom the cause of gallbladder wall thickening is otherwise not apparent.     

浅谈生物制品生产用原辅材料的质量控制

目前,生物制品的安全性和有效性正在引起社会公众的更广泛关注,而生物制品制造过程中所使用的原辅材料的质量对制品的安全性和稳定性有着直接的影响。因此,加强对此类原辅材料的质量控制对提高生物制品的质量将起到重要作用。     

数据挖掘技术在方剂配伍中的研究现状及研究方法

本文分析了数据挖掘技术在中医药研究领域以及方剂配伍中的研究现状,在此基础上对方剂配伍规律中数据挖掘技术的技术路线及研究内容进行了探讨,重点提出了几点数据挖掘技术在方剂配伍规律的研究方向。     

Time selection of acupuncture treatment for facial paralysis

目的：探讨针灸治疗面神经麻痹的最佳时机。方法：采用相同针法，对不同时期的面瘫患者86例进行疗效分析。治疗组各期均采用单纯常规毫针针刺，发展期刺激量小，静止期和恢复期加大刺激量；对照组发展期只给予改善微循环及皮质激素类药物，并辅以神经营养药治疗，静止期和恢复期治疗方法同治疗组。结果：治疗组痊愈率为88．1％，对照组为68．2％，而且治疗组痊愈所用时间也比对照组短。结论：说明早期针刺疗效优于西医常规治疗，而且疗程缩短。     

Changes in pyloric tumor of infantile hypertrophic pyloric stenosis before and after pyloromyotomy

Sixteen of 24 infants with vomiting were examined by real-time sonography, which established the diagnosis of hypertrophic pyloric stenosis (HPS); 12 underwent surgery. Of the three parameters measured, pyloric muscle wall thickness was the most reliable and accurate for the diagnosis of HPS. We suggest that a muscle wall thickness of 4 mm or more and stenosis index greater than 50% are standard findings in patients with HPS. Serial real-time sonography following pyloromyotomy showed that the pyloric tumor changed significantly during the 1st postoperative week and returned to normal size by the end of the 4th week. Offprint requests to: Z. X. Shen     

IL-12p40 is associated with type 1 diabetes in Caucasian-American families

The IL-12p40 locus has recently been shown to be associated with type 1 diabetes (1). Here, we report the identification of novel microsatellite and single-nucleotide polymorphisms (SNPs) within the IL-12p40 gene and a significant association between a (ATT)n repeat marker and type 1 diabetes in 364 U.S. Caucasian sib-pair families (P < 0.006). Haplotype analysis using the (ATT)n repeat (D5S2941) and the C1159A SNP at the 3' untranslated region of IL-12p40 showed a significant association (P = 0.02). Expression studies in individuals heterozygous for the C1159A SNP indicated that the expression of the 1159A allele is approximately 50% higher than that of the 1159C allele. These results provide genetic and functional evidence for IL-12p40 as a type 1 diabetes susceptibility gene.     

保持特色发挥优势促进中医药事业全面协调发展为人民健康服务 --在保持发挥中医药特色优势工作经验交流会上的讲话

国家中医药管理局于2005年12月8日至9日在北京召开了保持发挥中医药特色优势工作经验交流会。本刊转发卫生部副部长兼国家中医药管理局局长佘靖同志在会上所作的报告,供大家学习和贯彻。