Inhibition of lens-aldose reductase activity by brazilin and haematoxylin

Brazilin and haematoxylin, plant pigments, were examined for their effects on the Bovine-Lens aldose reductase (LAR)-activity. About 50% inhibition was observed in a concentration of 10 (-4) M-brazilin and 10 (-4) M-haematoxylin, and above 95% inhibition was observed in a concentration of 10 (-3) M-brazilin and 10 (-3)M-haematoxylin. In order to determine the type of inhibition, kinetic studies were also conducted with brazilin and haematoxylin, in which both were found to be noncompetitive inhibitors.     

肾综合征出血热多尿期肾图的变化及意义

目的 :研究肾综合征出血热 (HFRS)多尿期肾图的变化及其意义。方法 :静脉注射 1 31 I-邻碘马尿酸钠 (1 31 I- OIH) 3.7×10 9Bq(10 μCi) ,用闪烁照相法描记 15 min,通过电子计算机自动处理 ,得出肾图的各项参数。结果 :2 0例受检者均已进入多尿期 ,2 4h尿量在 2 0 0 0 ml以上 ,血尿素氮 (BUN)已正常或接近正常 ,但肾图却明显异常 ,表现为半排时间 (TC1 /2 )延长 (>8min) ,15min残留率 (C1 5 /b)超过 5 0 % ,肾脏指数大多低于 45 % ;同期作尿β2 -微球蛋白 (β2 - m )测定 ,也明显高于正常 ;8例作发射型电子计算机扫描 (ECT)检查 ,肾小球滤过率 (GFR)和有效肾血浆流量 (ERPF)分别为 (4 0 .8± 2 1.2 ) ml/min和 (35 0 .8± 10 2 .1) ml/min,均明显低于正常 (P<0 .0 1)。结论 :HFRS患者多尿期肾图大多呈排出不良和功能受损的图形 ,表明该期肾功能以及肾血流量尚未恢复正常 ,对症治疗仍需继续。     

以呕吐原因待查住院的疾病分析

目的 分析哎吐的发病情况,以期充分提高对其临床表现的认识,并提出合理的检查步骤。方法 采用回顾性方法分析了315例哎吐原因待查病例。结果 消化系统疾病与非消化系统疾病的比例分别为77．14％和20．23％,无论是消化系统疾病还是消化系统疾病,女性发病的比例均高于男性。结论 哎吐原因待查住院的病人病史较长病情复杂,可根据疾病的发病规律和临床特点,先将其分为消化系统疾病和非消化系统疾病两类,然后再根据各自的特点逐一分析,结合相应的检查最终明确诊断。     

竹黄通痹胶囊治疗痛风43例

目的：为寻求一种对治疗痛风病更为有效而且服用方便的方药。方法：门诊收治的43例患,根据临床表现,化验室检查和X线摄片而确诊。每日口服“竹黄通痹胶囊”连续服用3个月。结果：43例患临床痊愈30例,有效11例,无效2例,总有效率为95．4％。结论：竹黄通痹胶囊具有清热利湿,活血通络,消肿止痛等作用,且无不良反应。     

导电聚吡咯膜技术应用于大鼠肺细胞原代培养的实验研究

目的 :探讨导电聚吡咯膜技术对大鼠肺Ⅱ型细胞体外生长的作用。方法 :采用四甲基偶氮唑盐比色法测定比较导电聚吡咯膜组和常规培养组大鼠肺Ⅱ型细胞的生长和增殖情况。结果 :培养 3～ 5d后两组OD值有显著差异 (P <0 .0 5 )。结论 :常规培养条件下的大鼠肺Ⅱ型细胞在体外生长时间约 2～ 3d ,导电聚吡咯膜可以将肺Ⅱ型细胞体外生长时间延长至 5d。     

电磁辐射对小鼠神经系统超微结构影向的分析

目的探讨移动电话的电磁辐射对生物体的影响.方法用大众常用移动电话作为辐射源,工作频率为900MHz,功率密度为1190μW/cm2,在一定范围对小鼠辐射2h/d,30d后把小鼠断颈处死,取出大脑皮层、海马和小脑进行电镜观察分析.结果电镜所见,处理组与对照组小鼠的神经系统的细胞超微结构未见明显异常.结论一定时间内,移动电话的电磁辐射,对小鼠神经系统的细胞超微结构并没有明显影响.     

Study of the mechanism of essential garlic oil inhibiting lnterleukin-1 α-induced monocyte adhesion to endothelial cells

Objective  

To observe the effects of essential garlic oil (EGO) on vascular cell adhesive molecule-1 (VCAM-1) expression of endothelial cells and monocyte-endothelial cell adhesion rate induced by interleukin-l_α(IL-l_α).     

Comparative study of different transplantation methods of adipose tissue-derived stem cells in the treatment of erectile dysfunction caused by cavernous nerve injury

We aimed to compare intracavernosal injection (ICI), tail vein injection (IV), and periprostatic injection (PPI) of adipose-derived stem cells (ADSCs) for their ability to improve erectile function in cavernous nerve injury-induced erectile dysfunction (CNIED) rats and to explore the possible mechanism. Eighty-four male SD rats were divided into the sham group (n = 6), BCNI group (bilateral CN crush injury, n = 6), PBS-ICI group (n = 6), PBS-IV group (n = 6), PBS-PPI group (n = 6), ADSC-ICI group (n = 18), ADSC-IV group (n = 18) and ADSC-PPI group (n = 18). ADSCs were labelled with 5-ethynyl-2′-deoxyuridine (EdU), and six rats each in the ADSC-ICI group, ADSC-IV group, and ADSC-PPI group were sacrificed 2, 7, and 28 days after injection. EdU-labelled ADSCs were tracked by immunofluorescence staining. The intracavernosal pressure (ICP)/mean arterial pressure (MAP) ratio, neuronal nitric oxide synthase (nNOS)-positive nerve fibres in the dorsal penile nerve and the smooth muscle/collagen ratio in the cavernosum between groups were also evaluated. ADSCs can significantly improve erectile function through ICI or IV. The two are similar in efficacy and superior to PPI. The mechanism may be that after CN injury, ADSCs are recruited to around the MPG and secrete a variety of neurotrophic factors that promote the repair of the CN, thereby improving erectile function.     

In vivo imaging of neuroblastomas using GD2-targeting graphene quantum dots

BackgroundPatients with neuroblastoma, a common childhood malignancy, often have poor prognosis. It is mandatory to develop an accurate and efficient diagnostic tool for neuroblastomas, so that the treatment can be started early. Graphene quantum dot (GQD), a nanomaterial, can be used to carry proteins, genetic materials, or drugs. GD2, a disialoganglioside, is a surface antigen expressed on neuroblastoma. This study investigated the in vivo targeting and imaging of neuroblastomas using GD2-targeting GQDs.MethodsGQDs were synthesized and conjugated with anti-GD2 antibody (anti-GD2/GQDs). In vitro cytotoxicity of GQDs and anti-GD2/GQDs was studied in human neuroblastoma cells by 3-[4,5-dimethylthiazole-2-yl]-2,5-diphenyltetrazolium bromide)-based colorimetric assay. The tumor tracking and imaging of anti-GD2/GQDs in mice were investigated by in vivo imaging system (IVIS).ResultsTreatment with GQDs or anti-GD2/GQDs induced no or mild cytotoxicity in fibroblasts and neuroblastoma cells. After co-incubation, GQDs and anti-GD2/GQDs were located in the cytoplasm and nucleus of neuroblastoma cells, with GQDs showing a blue fluorescence and anti-GD2/GQDs an orange/red emission. The IVIS images demonstrated accumulation of the fluorescence of anti-GD2/GQDs in the subcutaneous tumors in mice 24 h after intravenous injection of anti-GD2/GQDs.ConclusionsAnti-GD2/GQDs may potentially be used for the targeting and imaging of neuroblastomas in vivo.