2004-2009年广西靖西县淋病、梅毒、AIDS/HIV流行状况分析

目的了解位于中越边境的靖西县2004-2009年梅毒、淋病、艾滋病感染及发病情况,分析其流行特征和规律,为制订性病艾滋病防治策略和措施提供科学依据。方法收集该县2004-2009年的梅毒、淋病、AIDS/HIV病例疫情资料,采用Excel软件进行统计分析。结果全县人口数为61.7万,近6年报告梅毒、淋病、AIDS/HIV阳性病例共767例,人群发病率为21.68/10万,男性高于女性,其中淋病118例、梅毒595例、艾滋病11例(死亡6例)、HIV阳性43例(死亡10例)。以上3种疾病高发年龄在15～44岁年龄组,占总发病率的68.06%。农民发病人数占59.06%。梅毒病例数2007年后增多明显。自1997年发现HIV感染者后,2004-2009年AIDS/HIV患者每年有所增加,病死率呈逐年上升,平均病死率为29.63%。结论该县2004-2009年梅毒患者增加较快,AIDS/HIV阳性病例有所增多,防治工作应进一步加强。     

开通闭塞冠状动脉策略的全球性研究 GUSTO—Ⅱb（亚组研究）

病人资料 3 289例因急性冠脉综合征而接受溶栓治疗的患者,2274例接受t－PA治疗,1015例接受链激酶治疗。     

Gastric insufflation during IAC-CPR and standard CPR in a canine model

This study was undertaken to determine the effect of interposed abdominal compressions (IAC) during cardiopulmonary resuscitation (CPR) on gastric insufflation when the airway is not secured with an endotracheal tube. A canine model was used in which a common ventilation pressure was applied to separate cuffed esophageal and tracheal tubes. Gas entering the stomach was collected by a pre-placed gastrostomy tube leading to a bell spirometer. Gas entering the lungs was measured with a Wright Respirometer in series with the endotracheal tube. During standard CPR, measurable gastric gas volume was collected in 28 of 30 trials (mean 215 +/- 93 ml/ventilation). During IAC-CPR, in which abdominal pressure was maintained during ventilation after every 5th chest compression, measurable gastric gas was collected in 15 of 30 trials (mean 40 +/- 11 ml/ventilation). Interposed abdominal compressions as an adjunct to standard CPR may not only be of hemodynamic benefit, but may also reduce the incidence of gastric insufflation and attendant complications.     

Interleukin-6 is not involved in the interleukin-1-induced production of colony-stimulating factors by human bone marrow stromal cells and fibroblasts

Interleukin-6 (IL-6) is a multifunctional cytokine that plays a role in regulation of hematopoiesis. Because IL-6 is coinduced with colony- stimulating factors (CSFs) by various cell types in response to stimulation with IL-1, we investigated whether IL-6 is involved in the IL-1-induced production of CSF by human bone marrow (BM) cells in long- term culture or human fibroblasts. We showed that IL-6 does not induce CSF production by these cells. Neither addition of exogenous IL-6 nor neutralization of endogenous production of IL-6 by an anti-IL-6 monoclonal antibody (MoAb) diminished the IL-1-induced colony- stimulating activity (CSA), indicating that IL-6 did not act synergistically with IL-1. Finally, IL-6 did not influence the kinetics of IL-1-induced CSA production by human fibroblasts. We conclude that IL-6, either alone or in combination with IL-1, does not induce CSF production by human BM stromal cells or fibroblasts.     

小藤铃儿草的生物碱成分

自小藤铃儿草Dactylicapnos torulosa(Hook.f ct Thomas.)Hutchins中分得六个单体,通过UV,IR,MS,~1HNMR和~(13)CNMR解析,鉴定了四个,其中一个为新化合物,命名为紫金龙碱(zijinlongine,Ⅰ),其他三个为已知化合物:氯化1-甲氧基小檗碱(1-methoxylberberium chloride,Ⅱ),氯化三乙基苯胺(triethylphcnylamium chloride,Ⅲ)和异紫堇定(isocorydine,Ⅳ)。Ⅱ和Ⅲ系首次从植物中得到。     

米非司酮抗人子宫内膜癌裸鼠移植瘤作用的实验研究

目的：观察米非司酮抗人子官内膜癌裸鼠移植瘤的作用，为临床应用提供依据。方法：将裸鼠分为米非司酮、三苯氧胺、米非司酮＋三苯氧胺、对照４组，用药并观察移植瘤生长情况，电镜观察移植瘤，测定裸鼠血清雌二醇。结果：米非司酮抗癌作用与三苯氧胺相似，二者联合作用增强。米非司酮使癌细胞坏死，内质网及线粒体肿胀，使裸鼠血清雌二醇升高。结论：米非司酮有抗子宫内膜癌作用，与三苯氧胺联合作用增强。     

Interleukin-4 induces a substance in bone marrow stromal cells that reversibly inhibits factor-dependent and factor-independent cell proliferation

Bone marrow-derived stromal cell monolayers pretreated with recombinant interleukin-4 (IL-4) inhibit the growth of hematopoietic cells. This was demonstrated by inhibition of fresh bone marrow-derived, IL-3- induced soft agar colonies as well as by inhibition of proliferation of IL-3-dependent cell lines and of a Friend virus-transformed erythroleukemic cell line. Pretreatment of stromal cells with IL-4 for five to seven days induced the inhibitory activity. IL-4 could then be removed before "plating" the bone marrow cells in soft agar, indicating that the inhibitory activity did not depend on the action of IL-4 on the precursors of the soft agar colonies. The inhibitory activity appears to be mediated by a soluble factor since inhibition was achieved even if the stromal cell layer was separated from the colony forming cells by an "empty" agar layer. However, supernatants of IL-4- induced stromal cell layers had no detectable inhibitory activity. The inhibitory action of the IL-4-pretreated stromal cell lines was not the result of killing of the precursor cells since it could be reversed if the agar layer containing the colony-forming cells was removed from the stromal cell layer and cultured with IL-3. Hydrocortisone (HC) blocked the inhibitory effect if added either in the IL-4 preincubation phase or during the colony formation stage, implying that HC blocked both induction of the inhibitory activity and its release or its effector function. A homogenous long-term stromal cell line could not be induced to exert the inhibitory activity; partial inhibition could be achieved with pure macrophages stimulated with IL-4 and CSF-1, suggesting that the inhibitory activity induced by IL-4 in mixed stromal cell layers may depend on a complex mechanism involving more than one cell type. Northern analysis of RNA from IL-4-induced and uninduced stromal cells indicated that IL-4 did not upregulate expression of CSF-1 or transforming growth factor-beta (TGF-beta) and only modestly increased expression of tumor necrosis factor, suggesting that these cytokines were not responsible for the inhibitory activity. The capacity of IL-4 to induce inhibitory activity in stromal cell layers suggests that IL-4 may play a role in the regulation of hematopoiesis.     

健康人心室复极的昼夜变化研究

目的 探讨健康人心室复极的昼夜变化规律. 方法 对34例健康体检者的24h动态心电图进行回顾性分析.测定其心率、Q-T间期、QRS波群起点至T波波峰的时间(Q-Tp间期).计算T波波峰至终点的时间(Tp-e时间)、心率校正的Q-T间期(Q-Tc间期)和Tp-e时间(Tp-ec时间)、Tp-e/Q-T值,并比较一天中8个等分时间段的上述参数. 结果 Q-T间期和Tp-e时间呈日间短、凌晨及午夜长的昼夜节律变化,各时间段差异有统计学意义(P<0.01或0.05);Q-T间期与心率呈高度负相关(r=-01796，P<0.01)．而Tp-e时间与心率呈低度负相关(r=-01267，P<0.01)；各时间段Q-Tc间期接近,而Tp-ec时间呈07:00～09:00最高、10:00～12:00次之、01:00～03:00最低的昼夜节律变化,各时间段差异有统计学意义(P<0.05),Tp-e／Q-T值昼夜变化节律与Tp-ec时间相近.结论 反映心室复极跨壁离散度的Tp-e时间除受心率影响外,尚受昼夜节律的影响.     

细胞因子在慢性肝衰竭合并全身炎症反应综合征病例中的意义

目的探讨TNF-α、IFN-γ、LPS、IL-10、IL-12、IL-18在慢性肝衰竭合并全身炎症反应综合征(SIRS)中的意义。方法运用双抗体夹心酶联免疫吸附法检测正常人、一般肝炎患者、慢性肝衰竭组的TNF-α、IFN-γ、LPS、IL-10、IL-12、IL-18。结果自正常对照组到一般肝炎组、慢性肝衰竭非SIRS组、慢性肝衰竭SIRS组,血清中TNF-α、LPS、IL-12及IL-18水平依次升高,IL-10分子水平依次减低,且各组间比较均具有显著性差异。结论TNF-α、LPS、IL-10、IL-12、IL-18水平可以反应出乙型肝炎患者的肝脏损伤程度和疾病严重程度,可能在慢性肝衰竭合并SIRS的发病过程中起到相当重要的作用。