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排序方式: 共有212条查询结果,搜索用时 15 毫秒
41.
荧光分光光度法测定姜黄制剂中的姜黄素 总被引:11,自引:0,他引:11
用荧光分光光度法测定姜黄胶囊制剂中的姜黄素。以四氢呋喃为溶剂,姜黄素的激发波长和发射波长分别为Ex=442nm,Em=475nm。姜黄素标准曲线的线性范围为0.010~0.40fg/ml。最低检测浓度5ng/ml;回收率100.00%±0.28%;RSD<1%。本法操作简便、快速、灵敏,适用于姜黄制剂中姜黄素的含量。 相似文献
42.
The zeta chain is a key component of the T cell antigen receptor (TCR-CD3) complex, required for the expression of the receptor on the cell surface. It contains an extremely small extracellular (EC) part of nine amino acids. Interestingly, the length, but not the sequence, of the zeta EC has been highly conserved through evolution. Here, we examined the effect of increasing the length of human zeta EC on TCR-CD3 assembly and surface expression. Appending a 30 kDa polypeptide to the N-terminus of zeta completely abolished assembly and transport of the TCR-CD3 to the cell surface. Addition of only 17 amino acids, including the HA-tag (HAzeta), strongly reduced the efficiency of TCR-CD3 assembly and led to reduced expression on the surface, suggesting that the short zeta EC region is located within the receptor complex. In Blue Native gels (BN-PAGE) these receptors had a normal size, indicating that they have a stoichiometry of alphabetagammaepsilondeltaepsilonzetazeta. In resting TCR-CD3s the HA-tag, and thus the zeta EC region, was not accessible for anti-HA antibody binding, demonstrating that it was indeed buried in a cavity within the receptor complex. However, prolonged stimulation with antigen permitted the access of the anti-HA antibody, thus suggesting that stimulation led to architectural changes in the TCR-CD3. 相似文献
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Gil J Busto EM Garcillán B Chean C García-Rodríguez MC Díaz-Alderete A Navarro J Reiné J Mencía A Gurbindo D Beléndez C Gordillo I Duchniewicz M Höhne K García-Sánchez F Fernández-Cruz E López-Granados E Schamel WW Moreno-Pelayo MA Recio MJ Regueiro JR 《The Journal of clinical investigation》2011,121(10):3872-3876
47.
On rare occasions, excessive lipase production by functioning pancreatic acinar cell carcinoma results in subcutaneous and intraosseous fat necrosis. A patient with subcutaneous nodules and osteolytic lesions from metastatic fat necrosis associated with this malignancy is reported. Prompt recognition of the syndrome led to complete resection of the otherwise asymptomatic neoplasm of the exocrine pancreas. 相似文献
48.
Christopher J Martin Paul Taylor Henry WW Potts 《BMC medical informatics and decision making》2008,8(1):49
Background
There is a need for a new cardiovascular disease model that includes a wider range of relevant risk factors, in particular lifestyle factors, to aid targeting of interventions and improve population models of the impact of cardiovascular disease and preventive strategies. The model needs to be applicable to a wider population including different ethnic groups, different countries and to those with and without cardiovascular disease. This paper describes the construction of the Cardiovascular Health Improvement Model that aims to meet these requirements. 相似文献49.
Rathke cleft cysts: CT, MR imaging, and pathologic features 总被引:1,自引:0,他引:1
The authors retrospectively reviewed the clinical, computed tomography (CT), and magnetic resonance (MR) imaging findings in seven patients with pathologically proved Rathke cleft cysts. All the cysts were located in the anterior sella turcica or the anterior suprasellar cistern. Five cysts had both intra- and suprasellar components, one was entirely intrasellar, and the other was predominantly suprasellar in location. The size of the cysts ranged from 8 to 20 mm. CT scans demonstrated low-density homogeneous lesions in four cases. On MR images of three of these four cases, the cysts had the same intensity as cerebrospinal fluid on T1- and T2-weighted images, while in the fourth case, the cyst was hyperintense on the T1-weighted images. In the remaining three cases, CT showed slight hyperdensity relative to brain parenchyma, suggestive of contrast enhancement. MR showed signal heterogeneity of these lesions with focal components of diminished signal intensity of T2-weighted images. These same foci appeared iso- to slightly hyperintense on T1-weighted images. 相似文献
50.
Papillary endolymphatic sac tumors: CT, MR imaging, and angiographic findings in 20 patients 总被引:6,自引:0,他引:6