全文获取类型
收费全文 | 610篇 |
免费 | 41篇 |
国内免费 | 9篇 |
专业分类
耳鼻咽喉 | 6篇 |
儿科学 | 15篇 |
妇产科学 | 7篇 |
基础医学 | 42篇 |
口腔科学 | 13篇 |
临床医学 | 80篇 |
内科学 | 136篇 |
皮肤病学 | 5篇 |
神经病学 | 29篇 |
特种医学 | 72篇 |
外科学 | 49篇 |
综合类 | 56篇 |
预防医学 | 71篇 |
眼科学 | 6篇 |
药学 | 32篇 |
中国医学 | 1篇 |
肿瘤学 | 40篇 |
出版年
2022年 | 5篇 |
2021年 | 5篇 |
2020年 | 12篇 |
2019年 | 12篇 |
2018年 | 8篇 |
2017年 | 6篇 |
2016年 | 6篇 |
2015年 | 13篇 |
2014年 | 13篇 |
2013年 | 17篇 |
2012年 | 22篇 |
2011年 | 19篇 |
2010年 | 18篇 |
2009年 | 26篇 |
2008年 | 19篇 |
2007年 | 27篇 |
2006年 | 21篇 |
2005年 | 19篇 |
2004年 | 34篇 |
2003年 | 19篇 |
2002年 | 26篇 |
2001年 | 22篇 |
2000年 | 17篇 |
1999年 | 14篇 |
1998年 | 19篇 |
1997年 | 25篇 |
1996年 | 12篇 |
1995年 | 10篇 |
1994年 | 9篇 |
1993年 | 7篇 |
1992年 | 10篇 |
1991年 | 10篇 |
1990年 | 5篇 |
1989年 | 11篇 |
1988年 | 14篇 |
1987年 | 10篇 |
1986年 | 11篇 |
1985年 | 9篇 |
1984年 | 6篇 |
1983年 | 14篇 |
1982年 | 10篇 |
1981年 | 5篇 |
1980年 | 7篇 |
1979年 | 4篇 |
1978年 | 5篇 |
1974年 | 6篇 |
1972年 | 5篇 |
1971年 | 4篇 |
1958年 | 11篇 |
1957年 | 4篇 |
排序方式: 共有660条查询结果,搜索用时 31 毫秒
31.
Four patients with Philadelphia (Ph') positive chronic myeloid leukemia (CML) were studied before, after, and on relapse following allogeneic bone marrow transplantation (BMT). Southern analysis of DNA from cells collected before and at relapse after BMT was performed in order to investigate the origin of the leukemia at relapse. Using minisatellite probes we showed that the relapse occurred in cells of host origin in all four patients and this was confirmed with a Y chromosome specific probe in two male patients who had a female donor. Furthermore, using two probes for the breakpoint cluster region (bcr) on chromosome 22, we showed that leukemic cells at relapse bore identical rearrangements to those in the disease at time of presentation of each patient. We conclude that relapse in all four patients is due to re-emergence of the original leukemic clone. 相似文献
32.
33.
Belson Rugwizangoga Theresia Mwabili Trishia Scanlan Peter Meyer James Kitinya 《African health sciences》2014,14(3):763-768
Background
Coats'' disease is an exudative retinal detachment with vascular telangiectasis occurring mostly in male children, the age group most affected by retinoblastoma.Objectives
Compare the differential diagnoses of Coats'' diseaseEstablish recommendation to early disease detection.Materials and Methods
A 3-year-old female child was referred to Muhimbili National Hospital (MNH), Tanzania, in September 2011. She had presented at the peripheral hospital with gradual onset of left eye leukocoria for 1 year and pain for 2 months. B-scan showed a mass in the left eye. A clinical diagnosis of retinoblastoma was made. Left eye enucleation was performed; the patient was referred to MNH, with the enucleated specimen.Results
Brain and orbits scan revealed no residual tumour. The globe measured 2x1.8 cm, the optic nerve stump measured 3 mm. A whitish mass filled the vitreous, with complete retinal detachment. Microscopy showed retinal gliosis, detachment with sub retinal PAS positive exudates, vacuolation and cholesterol clefts. Foreign body giant cells were present; telangiectatic thin-walled blood vessels were identified. Clinico-pathological findings were of stage 4 Coats'' disease.Conclusion
Coats'' disease is an important differential diagnosis of retinoblastoma. Delay to detect Coats'' disease leads to vision loss which necessitates eye enucleation as was in this child. 相似文献34.
Wolfgang J. Rettig Sai L. Su Sheila R. Fortunato Matthew J. Scanlan B. K. Mohan Raj Pilar Garin-Chesa John H. Healey Lloyd J. Old 《International journal of cancer. Journal international du cancer》1994,58(3):385-392
The human fibroblast activation protein (FAP) defined by monoclonal antibody (MAb) F19 is a cell surface antigen expressed in reactive stromal fibroblasts of breast, colorectal, lung and other epithelial cancers. In contrast to its stroma-specific localization in epithelial neoplasms, FAP is expressed in the malignant mesenchymal cells of bone and soft-tissue sarcomas. FAP is transiently expressed in some fetal mesenchymal tissues but is absent or expressed at low levels in most adult tissues. FAP is induced in cultured fibroblasts and, in these cells, consists of a Mr 95,000 subunit (FAPα) carrying the F19 epitope and a non-covalently bound Mr 105,000 subunit (FAPβ) lacking the F19 epitope. Using MAb F19 and 5 newly derived MAbs, we identify 3 distinct epitopes on FAPα and tentatively assign one epitope to FAPβ. Analysis of detergent extracts of a FAPαhighβ-sarcoma cell line by size exclusion–high performance liquid chromatography (HPLC) revealed that FAPα does not elute as a Mr, 95,000 species but as part of a high-molecular weight complex (Mr > 400,000) that dissociates into Mr 95,000 subunits in SDS gels. Immunoaffinity purification of FAPα followed by tryptic digestion, reversed-phase HPLC and microsequencing identified 3 unique FAPα peptides, with 2 showing sequence similarity (23/38 identical amino acids) to segments of CD26, a T-cell activation antigen. CD26 is a membrane-bound enzyme (dipeptidyl aminopeptidase IV), but immunopurified FAPα has little if any dipeptidase activity with typical CD26 substrates. Finally, studies with FAPlow leptomeningeal fibroblasts revealed that transforming growth factor-β, 12-O-tetradecanoyl phorbol-13-acetate and retinoids can upregulate FAP expression, whereas serum and several other factors had no or little effect on FAP levels. FAP and CD26 may belong to a family of structurally related but functionally distinct activation proteins that are expressed on different cell types and show unique modes of regulation in normal and malignant cells. 相似文献
35.
Cyanosis, clubbing, and arterial oxygen desaturation may occur in patients with liver disease, and are attributed to the presence of pulmonary or peripheral arterio-venous shunting. Cardiac catheterisation and angiocardiography in a patient with a normal heart did not demonstrate the presence of abnormal arterio-venous anastomoses. Pulmonary shunting was proved when intravenous technetium-labelled macroaggregated albumin, normally held up in capillary networks, was passed quickly through the lungs and immediately detected in high systemic blood flow organs. The opening of peripheral and pulmonary anastomoses in patients with liver disease may be owing to the presence of a vasodilatory substance such as ferritin, which was found to be abnormally increased in the patient's blood. 相似文献
36.
Identification of cancer/testis genes by database mining and mRNA expression analysis 总被引:11,自引:0,他引:11
37.
Jäger D Stockert E Jäger E Güre AO Scanlan MJ Knuth A Old LJ Chen YT 《Cancer research》2000,60(13):3584-3591
Characterization of immunogenic human melanoma antigens has been a major focus of tumor immunologists over the past two decades, and a broad array of antigens recognized by antibodies and T cells in the autologous host has been defined. In the present study, a melanoma library was screened by SEREX (serological analysis of cDNA expression libraries), and 43 genes were isolated, 2 of which, NY-MEL-1 and NY-MEL-3, encode novel gene products with differential tissue expression. NY-MEL-1 encodes a new rab GTP-binding protein, rab38. Among >40 rab proteins, rab38 has a unique COOH terminus which would allow posttranslational farnesylation and palmitoylation, lipid modifications normally occurring in ras proteins but not in other rab proteins. It is also the only rab gene showing a predominant mRNA expression in melanocytes, a cell-specific expression pattern likely related to melanosomal transport and docking. Northern blot analysis showed no detectable expression in other normal tissues. Consistent with this lineage specificity, rab38 mRNA is expressed in 80-90% of melanoma (17 of 19), but rarely in nonmelanocytic malignancies (1 of 16). The second novel gene isolated, NY-MEL-3, encodes a mitotic protein highly homologous to the Xenopus chromosome condensation protein XCAP-G, designated hCAP-G. Analysis of hCAP-G mRNA expression showed highest expression in the testis among normal tissues and variable expression in tumor cells, reflecting the proliferative activity in these cells. This mitosis-related expression suggests hCAP-G as a possible proliferation marker and a potential prognostic indicator in cancer. These findings provide further support that SEREX can define biologically significant molecules in cancer. 相似文献
38.
Parathyroid adenomas evaluated by Tl-201/Tc-99m pertechnetate subtraction scintigraphy and high-resolution ultrasonography 总被引:2,自引:0,他引:2
Winzelberg GG; Hydovitz JD; O'Hara KR; Anderson KM; Turbiner E; Danowski TS; Lippe RD; Melada GA; Harrison AM 《Radiology》1985,155(1):231-235
Thallium-201/technetium-99m pertechnetate subtraction scintigraphy of the parathyroid glands was performed in a prospective study of 33 patients who had undergone bilateral neck exploration for elevated serum calcium and serum parathyroid hormone levels. In 31 cases, the Tl-201/Tc-99m subtraction technique yielded an overall sensitivity of 81%, specificity of 99%, and accuracy of 94% for identifying solitary parathyroid adenomas. Tl-201/Tc-99m subtraction scintigraphy correctly identified 73% of parathyroid adenomas weighing less than 499 mg, 79% of those weighing 500-1,499 mg, and 100% of adenomas weighing more than 1,500 mg. In a subgroup of 24 patients with solitary parathyroid adenomas who underwent both scintigraphy and high-resolution sonography, the sensitivity, specificity, and accuracy of both procedures were similar. 相似文献
39.
40.