迷迭香酸抗血栓和抗血小板聚集作用

迷迭香酸是丹参水溶性成分之一。大鼠体内实验(iv)表明,它能抑制静脉血栓形成。阻抑胶原诱导的血小板聚集,促进纤维蛋白溶解活性。当剂量为50及100 mg/kg时,血栓形成的抑制率分别为41.9和54.8%(P<0.vv05)。当剂量为100及150mg/kg时,血小板聚集的抑制率分别为30.4%(P<0.05)和46.4%(P<0.01),血浆优球蛋白溶解时间缩短(P<0.05)。纤维蛋白原含量无明显变化。以上结果说明,迷迭香酸有温和的抗血栓作用。其机理可能与抗血小板聚集和增强纤维蛋白溶解活性有关。     

"Covert toxocariasis" in a child treated with low-dose diethylcarbamazine

A girl aged 2.5 years with "covert toxocariasis" was treated with low-dose diethylcarbamazine because of supposed noticeable disseminated Toxocara canis infection without ocular or visceral manifestations. There was marked blood and bone marrow eosinophilia, significant increased Toxocara canis antibody (ELISA) and immunoglobulins E, G and M, leucocytosis and an increased sedimentation rate. She had no geophagia, but often sucked small stones, probably contaminated with faeces from puppies. Symptoms were fever, inactivity, weakness, tiredness and loss of appetite. She was followed clinically and with blood samples throughout a period of three years and four months.     

单鼻孔直入经筛骨垂直板后段经蝶入路切除垂体腺瘤

目的 介绍、推广单鼻孔直入经筛骨垂直板后段经蝶入路切除垂体腺瘤的手术经验，探讨该术式的优越性。方法 2001年10月以来对43例垂体腺瘤采用单鼻孔直入经筛骨垂直板后段经蝶入路手术。结果 本组无手术死亡，平均住院时间1周。随访3～25个月。MR复查示肿瘤全切除32例，近全切除9例，大部分切除2例。有内分泌症状的37例中．10例恢复正常，15例改善，12例同术前。结论 单鼻孔直入经筛骨垂直板后段经蝶手术最大限度地利用了鼻腔的自然间隙，入路直接．手术时间短．创伤轻微．并发症较少．是目前最为理想的经蝶手术术式．     

多联袋无菌制备脐带血间质干细胞的方法及效果

目的:分析人脐带血间质干细胞分离培养方法及临床应用的安全性。方法:实验于2005-04/12在河南省红十字血液中心和郑州市第二人民医院完成。实验方法:①无菌条件下将脐带血液采集到无菌采血袋内,产妇及其家属均知情同意,并经医院伦理委员会批准。随机抽查62份样本,单份采集量80～160mL,计数有核细胞。②以1:1体积比将Ficoll-Hypaque混合溶液加入四联袋主袋内,将四联袋与脐带血收集袋相连,再将样本血液沿袋壁缓慢加入四联袋主袋内的液面上。离心后将四联袋主袋放在分浆夹上,袋内液体自上而下分为血浆血小板层、单个核细胞层、淋巴细胞分离液层、多核细胞层、红细胞层5层。将血浆血小板层上2/3液体挤压入四联袋一空袋内,将剩余的血浆血小板层、单个核细胞层、淋巴细胞分离液层上部挤压入四联袋另一空袋内,即为富含有核细胞液体。③随机选择本院2005-04/12收治脑卒中后遗症患者10例。男9例,女1例,35～75岁,病程3个月～7年。诊断均由CT、MRI检查证实,符合第四届全国脑血管病学术会议修订的脑卒中诊断标准,患者均对治疗方案知情同意。将脐带血间充质干细胞经静脉输注入患者体内,每次输注1份,每例患者平均输注6份,每份间隔时间平均4d。于治疗前和治疗3个月后评价患者神经功能状态、肢体运动功能及日常生活活动能力。采用脑卒中患者临床神经功能缺损程度评分标准评估神经功能,分数越低,神经功能状态越好;采用Fugl-Meyer评估患侧肢体运动功能,分数越高,肢体运动功能越好;采用Barthel指数评估日常生活活动能力,分数越高,日常生活活动能力越高。结果:①分离的62份脐血间质干细胞,每份含有有核细胞(3.62±2.39)×109,红细胞残余量为(1.64±1.25)×109,CD34 细胞的比率为(2.31±0.93)%,CD133 的比率为(1.36±1.23)%。培养7d后贴壁细胞为(3.27±1.85)×107,CD133 比率为(8.21±2.46)%。②患者治疗前与治疗3个月后相比,神经功能状态、肢体运动功能及日常生活活动能力评分均明显升高(治疗前:25.40±6.09,31.90±21.85,36.20±19.41;治疗后:1.90±5.09,80.9±25.00,81.10±15.93,P<0.01)。未发现免疫排斥及不良反应。结论:①利用多联袋无菌制备脐血间质干细胞分离效果良好,分离过程在密闭系统进行,单个核细胞和间质干细胞能满足临床需要,适合患者静脉输注和局部直接应用,方法简便安全可靠。②经静脉输注后,疗效确切,能明显改善脑卒中后遗症作用。     

The collection and evaluation of peripheral blood progenitor cells sufficient for repetitive cycles of high-dose chemotherapy support

BACKGROUND: The development of an optimized peripheral blood progenitor cell (PBPC) harvest protocol to provide support for repetitive chemotherapy cycles is described. STUDY DESIGN AND METHODS: PBPCs mobilized by cyclophosphamide plus granulocyte-colony-stimulating factor (G-CSF) were studied in 163 leukapheresis harvests from 26 lymphoma patients. Harvested cells were transfused with two chemotherapy cycles and with an autologous bone marrow transplant. Progenitor cell content was examined in the context of hematopoietic engraftment. RESULTS: Mobilization allowed the harvest of large numbers of PBPCs. Peak harvests tended to occur after the recovering white cell count exceeded 10 × 10(9) per L. CD34+ lymphomononuclear cell (MNC) and colony-forming units-granulocyte-macrophage (CFU-GM) counts correlated poorly, but both measures peaked within 24 hours of each other in 21 of 26 patients, which demonstrated PBPC mobilization. Engraftment of platelets (> 50×10(9)/L) and granulocytes (> 500×10(6)/L) was achieved in a median of 20.5 and 16 days, respectively. A minimum number of progenitors necessary to ensure engraftment could be derived. CONCLUSION: Cyclophosphamide and G-CSF allowed the harvest of sufficient PBPCs to support multiple rounds of chemotherapy. Harvest should commence when the recovery white cell count exceeds 10×10(9) per L. PBPC harvest CD34+MNC counts are as useful as CFU-GM results in the assessment of PBPC content, and they may allow harvest protocols to be tailored to individual patients.     

雄激素受体基因第一外显子区CAG缺失的构建及其在HEK293细胞中的表达

目的：构建雄激素受体基因第一外显子区CAG重复序列缺失的突变重组体，观察CAG重复序列的有，无对雄激素受体转录及表达的调控作用。方法：实验于2006—05／2007—05在解放军总医院老年医学研究所完成。按照重叠延伸反应的原理扩增CAG重复序列两端的基因片段，再将两片段混合，在加入一个引物的情况下进行8个PCR循环以有效完成重叠延伸，然后再加入另一引物进行22个循环，完成目的片段指数扩增，将目的片段克隆至真核表达载体PAR-IRES2-EGFP，转染HEK293细胞，采用Western blot法检测HEK293细胞雄激素受体蛋白。结果：构建的CAG重复序列缺失的突变重组体经酶切鉴定和DNA测序证实构建正确，转染真核细胞可检测到雄激素受体基因的表达。结论：重叠延伸PCR是进行体外基因拼接、体外缺失突变的简单、快速的基因重组技术；雄激素受体基因第一外显子区CAG重复序列缺失的突变重组体的成功构建可为今后的相关研究提供实验基础。