Hoferellus azevedoi n. sp. was found in the urinary bladder of Chaetobranchusflavescens Heckel, 1840 from the Arari River on Marajó Island in Pará, Brazil. This is the first record of a species of the genus Hoferellus in a host from the Brazilian Amazon region. The new species has disporous and polysporous plasmodia, which vary in size and shape, with some being found adhered to the epithelium of the urinary bladder, and others floating in the liquid. The mature spores are sub-spherical in the sutural view, with a number of peripheral projections around the whole surface of the spore. In the sutural view, the spores are 5.3 ± 0.2 (5.2–5.6) μm in length and 7.0 ± 0.7 (6.3–7.7) μm in width, with two piriform polar capsules of equal size, 2.5 ± 0.2 (2.3–2.8) μm long and 1.8 ± 0.2 (1.6–2.0) μm wide. Based on a partial (1312 bps) sequence of the SSU rDNA gene, Hoferellus azevedoi n. sp. was distinguished from all the other myxozoan species deposited in GenBank. Phylogenetically, based on Bayesian inference and p-distances, the new species was allocated to the “Freshwater Urinary-Bladder” clade, together with other myxozoan parasites of the excretory system. Based on the morphological data, supported by the partial sequence of the SSU rDNA gene, we describe a new species of myxozoan, Hoferellus azevedoi n. sp. 相似文献
Euchroma Dejean, 1833 (Buprestidae: Coleoptera) is a monotypic genus comprising the species Euchroma gigantea, with populations presenting a degree of karyotypic variation/polymorphism rarely found within a single taxonomic (specific) unit, as well as drastically incompatible meiotic configurations in populations from extremes of the species range. To better understand the complex karyotypic evolution of E. gigantea, the karyotypes of specimens from five populations in Brazil were investigated using molecular cytogenetics and phylogenetic approaches. Herein, we used FISH with histone genes as well as sequencing of the COI to determine differential distribution of markers and relationships among populations. The analyses revealed new karyotypes, with variability for chromosome number and morphology of multiple sex chromosome mechanisms, occurrence of B chromosome variants (punctiform and large ones), and high dispersion of histone genes in different karyotypes. These data indicate that chromosomal polymorphism in E. gigantea is greater than previously reported, and that the species can be a valuable model for cytogenetic studies. The COI phylogenetic and haplotype analyses highlighted the formation of three groups with chromosomally polymorphic individuals. Finally, we compared the different karyotypes and proposed a model for the chromosomal evolution of this species. The species E. gigantea includes at least three cytogenetically polymorphic lineages. Moreover, in each of these lineages, different chromosomal rearrangements have been fixed. Dispersion of repetitive sequences may have favored the high frequency of these rearrangements, which could be related to both adaptation of the species to different habitats and the speciation process. 相似文献
Dengue is an infectious viral disease transmitted by the Aedes aegypti mosquito, the control of which is complex. In addition, the clinical diagnosis is difficult to perform since it resembles other febrile infections; thus, the development of more effective methods to detect dengue virus (DV) has drawn increasing attention. The present study aimed to develop an impedimetric immunosensor for dengue diagnosis using a screen-printed electrode (SPE) functionalized with polymer films derived from 4-aminophenylacetic acid (4-APA). Data obtained from scanning electron microscopy (SEM) showed the deposition of a uniformly distributed material over the electrode surface. The immunosensor was based on the specific interaction between dengue antigen, NS1 protein, and anti-NS1 antibodies, IgG and IgM. In a characterization study using cyclic voltammetry (CV), the polymer film showed two oxidation peaks at +0.17 and?+?0.35 V in 0.50 M sulfuric acid solution, indicating its adsorption and electroactivity at the SPE surface. Electrochemical impedance spectroscopy (EIS) measurements showed a higher charge transfer resistance (Rct) to the polymer film-modified SPE as compared with the bare SPE, corroborating a previous study. The best rNS1 concentration for immobilization was 1.00 ng/mL, and the immunoreaction time between the antigen (Ag) and the antibody (Ab) was 20 min. Dilutions of positive and negative clinical serum samples were evaluated by EIE, from which it was possible to elucidate, for the positive serum, that the more diluted the serum the greater the Rct. Negative serum also showed an analytical signal, probably due to the presence of non-specific antibodies; however, the generated signal presented values closer to the rNS1 signal, indicating good selectivity of the proposed platform. The experiments were repeated using bare SPE to verify the importance of the polymer film in biosensor construction. No significant difference was observed between these results.
An unclear issue is whether gender may influence at cardiac remodeling after
myocardial infarction (MI). We evaluated left ventricle remodeling in female
and male rats post-MI.
Methods
Rats were submitted to anterior descending coronary occlusion.
Echocardiographic evaluations were performed on the first and sixth week
post-occlusion to determine myocardial infarction size and left ventricle
systolic function (FAC, fractional area change). Pulsed Doppler was applied
to analyze left ventricle diastolic function using the following parameters:
E wave, A wave, E/A ratio. Two-way ANOVA was applied for comparisons,
complemented by the Bonferroni test. A P≤=0.05 was
considered significant.
Results
There were no significant differences between genders for morphometric
parameters on first (MI [Female (FE): 44.0±5.0 vs. Male (MA): 42.0±3.0%];
diastolic [FE: 0.04±0.003 vs. MA: 0.037±0.005, mm/g] and systolic [FE:
0.03±0.0004 vs. MA: 0.028±0.005, mm/g] diameters of left ventricle) and
sixth (MI [FE: 44.0±5.0 vs. MA: 42.0±3.0, %]; diastolic [FE: 0.043±0.01 vs.
MA: 0.034±0.005, mm/g] and systolic [FE: 0.035±0.01 vs. MA: 0.027±0.005,
mm/g] of LV) week. Similar findings were reported for left ventricle
functional parameters on first (FAC [FE: 34.0±6.0 vs. MA: 32.0±4.0, %]; wave
E [FE: 70.0±18.0 vs. MA: 73.0±14.0, cm/s]; wave A [FE: 20.0±12.0 vs. MA:
28.0±13.0, cm/s]; E/A [FE: 4.9±3.4 vs. MA: 3.3±1.8]) and sixth (FAC [FE:
29.0±7.0 vs. MA: 31.0±7.0, %]; wave E [FE: 85.0±18.0 vs. MA: 87.0±20.0,
cm/s]; wave A [FE: 20.0±11.0 vs. MA: 28.0±17.0, cm/s]; E/A [FE: 6.2±4.0 vs.
MA: 4.6±3.4]) week.
Conclusion
Gender does not influence left ventricle remodeling post-MI in rats. 相似文献
To determine whether stratification of complexity models in congenital heart
surgery (RACHS-1, Aristotle basic score and STS-EACTS mortality score) fit
to our center and determine the best method of discriminating hospital
mortality.
Methods
Surgical procedures in congenital heart diseases in patients under 18 years
of age were allocated to the categories proposed by the stratification of
complexity methods currently available. The outcome hospital mortality was
calculated for each category from the three models. Statistical analysis was
performed to verify whether the categories presented different mortalities.
The discriminatory ability of the models was determined by calculating the
area under the ROC curve and a comparison between the curves of the three
models was performed.
Results
360 patients were allocated according to the three methods. There was a
statistically significant difference between the mortality categories:
RACHS-1 (1) - 1.3%, (2) - 11.4%, (3)-27.3%, (4) - 50 %,
(P<0.001); Aristotle basic score (1) - 1.1%, (2) -
12.2%, (3) - 34%, (4) - 64.7%, (P<0.001); and STS-EACTS
mortality score (1) - 5.5 %, (2) - 13.6%, (3) - 18.7%, (4) - 35.8%,
(P<0.001). The three models had similar accuracy by
calculating the area under the ROC curve: RACHS-1- 0.738; STS-EACTS-0.739;
Aristotle- 0.766.
Conclusion
The three models of stratification of complexity currently available in the
literature are useful with different mortalities between the proposed
categories with similar discriminatory capacity for hospital mortality. 相似文献
