全文获取类型
收费全文 | 3256篇 |
免费 | 260篇 |
国内免费 | 17篇 |
专业分类
耳鼻咽喉 | 13篇 |
儿科学 | 66篇 |
妇产科学 | 37篇 |
基础医学 | 605篇 |
口腔科学 | 24篇 |
临床医学 | 397篇 |
内科学 | 806篇 |
皮肤病学 | 78篇 |
神经病学 | 264篇 |
特种医学 | 39篇 |
外科学 | 340篇 |
综合类 | 3篇 |
一般理论 | 1篇 |
预防医学 | 250篇 |
眼科学 | 50篇 |
药学 | 251篇 |
中国医学 | 1篇 |
肿瘤学 | 308篇 |
出版年
2023年 | 26篇 |
2022年 | 45篇 |
2021年 | 110篇 |
2020年 | 58篇 |
2019年 | 86篇 |
2018年 | 114篇 |
2017年 | 67篇 |
2016年 | 94篇 |
2015年 | 104篇 |
2014年 | 130篇 |
2013年 | 176篇 |
2012年 | 276篇 |
2011年 | 310篇 |
2010年 | 159篇 |
2009年 | 149篇 |
2008年 | 251篇 |
2007年 | 241篇 |
2006年 | 236篇 |
2005年 | 244篇 |
2004年 | 216篇 |
2003年 | 171篇 |
2002年 | 165篇 |
2001年 | 13篇 |
2000年 | 5篇 |
1999年 | 12篇 |
1998年 | 20篇 |
1997年 | 17篇 |
1996年 | 8篇 |
1995年 | 18篇 |
1994年 | 5篇 |
1993年 | 3篇 |
1992年 | 2篇 |
1991年 | 1篇 |
1988年 | 1篇 |
排序方式: 共有3533条查询结果,搜索用时 15 毫秒
141.
142.
Silvia Ramos-Campoy Anna Puiggros Sílvia Be Sandrine Bougeon María Jos Larryoz Dolors Costa Helen Parker Gian Matteo Rigolin Margarita Ortega María Laura Blanco Rosa Collado Rocío Salgado Tycho Baumann Eva Gimeno Carolina Moreno Francesc Bosch Xavier Calvo María Jos Calasanz Antonio Cuneo Jonathan C. Strefford Florence Nguyen-Khac David Oscier Claudia Haferlach Jacqueline Schoumans Blanca Espinet 《Haematologica》2022,107(3):593
Genome complexity has been associated with poor outcome in patients with chronic lymphocytic leukemia (CLL). Previous cooperative studies established five abnormalities as the cut-off that best predicts an adverse evolution by chromosome banding analysis (CBA) and genomic microarrays (GM). However, data comparing risk stratification by both methods are scarce. Herein, we assessed a cohort of 340 untreated CLL patients highly enriched in cases with complex karyotype (CK) (46.5%) with parallel CBA and GM studies. Abnormalities found by both techniques were compared. Prognostic stratification in three risk groups based on genomic complexity (0-2, 3-4 and ≥5 abnormalities) was also analyzed. No significant differences in the percentage of patients in each group were detected, but only a moderate agreement was observed between methods when focusing on individual cases (κ=0.507; P<0.001). Discordant classification was obtained in 100 patients (29.4%), including 3% classified in opposite risk groups. Most discrepancies were technique-dependent and no greater correlation in the number of abnormalities was achieved when different filtering strategies were applied for GM. Nonetheless, both methods showed a similar concordance index for prediction of time to first treatment (TTFT) (CBA: 0.67 vs. GM: 0.65) and overall survival (CBA: 0.55 vs. GM: 0.57). High complexity maintained its significance in the multivariate analysis for TTFT including TP53 and IGHV status when defined by CBA (hazard ratio [HR] 3.23; P<0.001) and GM (HR 2.74; P<0.001). Our findings suggest that both methods are useful but not equivalent for risk stratification of CLL patients. Validation studies are needed to establish the prognostic value of genome complexity based on GM data in future prospective studies. 相似文献
143.
Leslie Calapre Elin S. Gray Sandrine Kurdykowski Anthony David Pascal Descargues Mel Ziman 《BMC dermatology》2017,17(1):8
Background
Exposure to heat stress after UVB irradiation induces a reduction of apoptosis, resulting in survival of DNA damaged human keratinocytes. This heat-mediated evasion of apoptosis appears to be mediated by activation of SIRT1 and inactivation of p53 signalling. In this study, we assessed the role of SIRT1 in the inactivation of p53 signalling and impairment of DNA damage response in UVB plus heat exposed keratinocytes.Results
Activation of SIRT1 after multiple UVB plus heat exposures resulted in increased p53 deacetylation at K382, which is known to affect its binding to specific target genes. Accordingly, we noted decreased apoptosis and down regulation of the p53 targeted pro-apoptotic gene BAX and the DNA repair genes ERCC1 and XPC after UVB plus heat treatments. In addition, UVB plus heat induced increased expression of the cell survival gene Survivin and the proliferation marker Ki67. Notably, keratinocytes exposed to UVB plus heat in the presence of the SIRT1 inhibitor, Ex-527, showed a similar phenotype to those exposed to UV alone; i.e. an increase in p53 acetylation, increased apoptosis and low levels of Survivin.Conclusion
This study demonstrate that heat-induced SIRT1 activation mediates survival of DNA damaged keratinocytes through deacetylation of p53 after exposure to UVB plus heat144.
145.
146.
147.
148.
Sandrine Chometton Solène Pedron Yvan Peterschmitt Vincent Van Waes Dominique Fellmann Pierre-Yves Risold 《Brain structure & function》2016,221(4):2183-2208
The lateral hypothalamic area (LHA) has two major roles: arousal/waking and food intake controls. Here, it is shown that a premammillary part of the LHA is neurochemically and cytoarchitectonically distinct from the tuberal LHA in male rats. This part contains nuclear masses, namely the parasubthalamic nucleus and the calbindin nucleus, involved in pathways that predict its participation in the control of food intake. Analyzing c-Fos expression in experiments related to feeding behavior, this region responded specifically to the ingestion of palatable nutriments. 相似文献
149.
Paul Calès Jérôme Boursier Julien Chaigneau Fabrice Lainé Jeremy Sandrini Sophie Michalak Isabelle Hubert Nina Dib Frédéric Oberti Sandrine Bertrais Gilles Hunault Christine Cavaro‐Ménard Yves Gallois Yves Deugnier Marie C. Rousselet 《Liver international》2010,30(9):1346-1354
Aims: Our aim was to develop an accurate, non‐invasive, blood‐test‐based method for identifying the main characteristics of liver fibrosis in non‐alcoholic fatty liver disease (NAFLD). Methods: Fibrosis was staged according to NASH‐CRN and Metavir systems in 226 patients with NAFLD. A fully automated algorithm measured the fractal dimension (FD) and the area of fibrosis (AOF). Independent predictors of diagnostic targets were determined using bootstrap methods. Results: (i) Development. Significant fibrosis defined by NASH‐CRN F≥2 was diagnosed by weight, glycaemia, aspartate aminotransferase (AST), alanine aminotransferase (ALT) and prothrombin index [area under the receiver operating characteristic (AUROC)=0.867]; significant fibrosis defined by Metavir F≥2 was diagnosed by weight, age, glycaemia, AST, ALT, ferritin and platelets (FibroMeter AUROC=0.941, P<0.005). AOF was estimated by the combination of hyaluronic acid, glycaemia, AST, ALT, platelets and prothrombin index (aR2=0.530), while FD was estimated by hyaluronic acid, glycaemia, AST/ALT, weight and platelets (aR2=0.529). (ii) Evaluation. Although NASH‐CRN was a better system for fibrosis staging, Metavir staging was a better reference for blood test. Thus, the patient rate with predictive values≥90% by tests was 97.3% with Metavir reference vs. 66.5% with NASH‐CRN reference (P<10?3). FibroMeter showed a significantly higher AUROC than the NAFLD fibrosis score for significant fibrosis, but not for severe fibrosis or cirrhosis, with both staging systems. Relationships between fibrosis lesions were well reflected by blood tests, e.g., the correlation between histological area and FD of fibrosis (rs=0.971, P<10?3) was well reflected by the relationship between respective blood tests (rs=0.852, P<10?3). Conclusions: Different characteristics of fibrosis in NAFLD can be diagnosed and quantified by blood tests with excellent accuracy. 相似文献
150.
Sandrine Cappellesso-Fleury Bénédicte Puissant-Lubrano Pol-André Apoil Matthias Titeux Peter Winterton Louis Casteilla Philippe Bourin Antoine Blancher 《Journal of clinical immunology》2010,30(4):607-619