全文获取类型
收费全文 | 316篇 |
免费 | 45篇 |
国内免费 | 1篇 |
专业分类
儿科学 | 15篇 |
妇产科学 | 2篇 |
基础医学 | 49篇 |
口腔科学 | 6篇 |
临床医学 | 45篇 |
内科学 | 107篇 |
皮肤病学 | 2篇 |
神经病学 | 11篇 |
特种医学 | 41篇 |
外科学 | 20篇 |
综合类 | 1篇 |
预防医学 | 12篇 |
眼科学 | 15篇 |
药学 | 7篇 |
肿瘤学 | 29篇 |
出版年
2023年 | 2篇 |
2022年 | 1篇 |
2021年 | 7篇 |
2020年 | 1篇 |
2019年 | 1篇 |
2018年 | 8篇 |
2017年 | 3篇 |
2016年 | 9篇 |
2015年 | 8篇 |
2014年 | 7篇 |
2013年 | 11篇 |
2012年 | 23篇 |
2011年 | 14篇 |
2010年 | 13篇 |
2009年 | 9篇 |
2008年 | 10篇 |
2007年 | 14篇 |
2006年 | 6篇 |
2005年 | 11篇 |
2004年 | 8篇 |
2003年 | 12篇 |
2002年 | 9篇 |
2001年 | 8篇 |
2000年 | 11篇 |
1999年 | 9篇 |
1998年 | 12篇 |
1997年 | 11篇 |
1996年 | 12篇 |
1995年 | 15篇 |
1994年 | 8篇 |
1993年 | 11篇 |
1992年 | 8篇 |
1991年 | 11篇 |
1990年 | 3篇 |
1989年 | 9篇 |
1988年 | 8篇 |
1987年 | 3篇 |
1986年 | 3篇 |
1985年 | 4篇 |
1984年 | 1篇 |
1983年 | 2篇 |
1982年 | 5篇 |
1981年 | 4篇 |
1980年 | 6篇 |
1977年 | 5篇 |
1976年 | 1篇 |
1975年 | 4篇 |
1974年 | 1篇 |
排序方式: 共有362条查询结果,搜索用时 15 毫秒
61.
62.
63.
64.
PURPOSE: To assess the relationship between objective tests of visual function and vision-related quality of life in patients with Bothnia dystrophy (BD), a retinal dystrophy of retinitis pigmentosa type with progressive maculopathy. METHODS: Forty-nine patients were tested. Weighted distance logMAR visual acuity (WVA), weighted logMAR low contrast VA (WCS), and binocular visual field (VF) areas were calculated. Vision-related quality of life (VRQL) was assessed using the 25-item National Eye Institute-Visual Function Questionnaire (NEI-VFQ-25). Correlation statistics were used and adjusted analyses of the relationship between the composite score and the objective visual function tests were performed with multiple linear regression. RESULTS: VRQL was significantly correlated with age, WVA, WCS, and binocular VF areas (P < 0.001). Calculation of partial correlation coefficients showed age to be significantly correlated only with VF (V-4-e) area (P < 0.0001). Multiple linear regression analyses revealed age and WVA to be significantly associated with the NEI-VFQ-25 composite score (P < 0.02 and P = 0.001, respectively). WVA alone was the strongest predictor of self-reported experience of total visual function in BD patients (r 2= 0.69). CONCLUSIONS: A strong relationship between objective tests of visual function and patient perceived VRQL as assessed by a questionnaire was found. WVA was the strongest predictor and together with age explained almost 70% of the variability of the composite score of the questionnaire. 相似文献
65.
Matsusaka T Sandgren E Shintani A Kon V Pastan I Fogo AB Ichikawa I 《Journal of the American Society of Nephrology : JASN》2011,22(7):1275-1285
Loss of podocytes promotes glomerulosclerosis, but whether this results from a continued primary insult or a secondary mechanism triggered by the initial loss of podocytes is unknown. We generated chimeric mice in which only a subpopulation of podocytes expressed hCD25, which is the receptor for the immunotoxin LMB2. In addition, genetic labeling of hCD25-negative cells with human placental alkaline phosphatase allowed the study of these two distinct podocyte populations. Administration of LMB2 did not cause podocyte injury in hCD25-negative control mice. In contrast, LMB2 severely damaged or sloughed off the subpopulation of hCD25-positive podocytes within the chimeric glomeruli. Moreover, hCD25-negative podocytes, which were immune to the initial toxin injury, developed injury as early as 4 d after LMB2 injection, evidenced by foot process effacement, upregulation of desmin, and downregulation of nephrin, podocin, and podocalyxin. Furthermore, the magnitude of secondary injury correlated with the magnitude of primary injury, supporting the concept of an amplified cascade of podocyte injury. In conclusion, podocyte damage can propagate injury by triggering secondary damage of "remnant" intact podocytes, even when the primary insult is short-lived. This transmission of podocyte injury may form a vicious cycle leading to accelerated podocyte deterioration and glomerulosclerosis. 相似文献
66.
Ainars Reinis Irina Golovleva Linda Köhn Ola Sandgren 《Acta ophthalmologica. Supplement》2013,91(3):259-266
Purpose: To describe in detail the phenotype of CORD5 in two families segregating a mutation c.1878G>C (p.Q626H) in the PITPNM3 gene. Methods: The study included 35 individuals from two different families of Swedish origin, all heterozygous for a PITPNM3 p.Q626H mutation. All participants underwent ophthalmological examination including kinetic perimetry, and in selected cases adaptometry, colour vision tests and optical coherence tomography (OCT). Electrophysiological studies were also performed. In some cases, the data were obtained from medical records. Results: The majority of patients showed subnormal visual acuity and light sensitivity from childhood. Early signs of macular degeneration were also observed. There was a progressive decrease in visual acuity leading to legal blindness in early adulthood. Electrophysiological testing showed a progressive loss of photoreceptor function restricted mainly to the cones. OCT revealed decreased macular thickness with flattened and enlarged fovea. Conclusion: Our observations of the PITPNM3 p.Q626H mutation carriers confirm that CORD5 is a disease not to mix with other retinal degenerations mapped to 17p13. The results of our clinical evaluation so far indicate that CORD5 is characterized by predominant cone dysfunction without signs of general involvement of the retinal pigment epithelium. The rod system also seems to be unaffected. In this sense, CORD5 is different from other autosomal dominant CORDs where rod involvement is present to some degree in a late phase of the disease. Some intra‐ and inter‐familial differences regarding the severity of the clinical picture were observed. 相似文献
67.
Ulf Ekström Sten Andréasson Vesna Ponjavic Magnus Abrahamson Ola Sandgren Peter Nilsson–Ehle 《Ophthalmic genetics》2013,34(3):149-156
Purpose: To clinically characterize a Swedish family with autosomal dominant retinitis pigmentosa due to a mutation, Arg-172-Trp, in the peripherin/RDS gene. Methods: Full clinical evaluation including kinetic visual field testing, measurement of dark-adaptation threshold, and full-field electroretinography in seven patients with autosomal dominant retinitis pigmentosa and three healthy family members. Denaturing gradient gel electrophoresis (DGGE) was used for mutation screening in seven patients and six healthy members of the family. Results: Three of four siblings from the middle generation and four of the younger generation were heterozygous for the peripherin/RDS Arg-172-Trp mutation. The mutation segregated with the disease. Visual acuity decreased progressively with age and visual fields were moderately constricted in young patients, while central scotoma and constriction of the fields were detected in the family members above 50 years of age. The results from full-field electrography were comparable with a widespread retinal degeneration. Conclusions: Earlier, the peripherin/RDS Arg-172-Trp mutation was associated primarily with a macular degeneration phenotype. One previous study indicated that this mutation also can give rise to a degeneration of the more peripheral parts of the retina. In the present study, a widespread retinal degeneration is seen in the patients above 50 years of age, carrying the Arg-172-Trp mutation. 相似文献
68.
Radiology conferences enable participants the opportunity to ask experts questions through question and answer (Q and A) sessions
or individually. Given the time limitations and intimidating circumstances, we incorporated conference text messaging (confexting)
as a method of increasing interactivity between the audience and speakers. During a 5-day radiology conference, text messaging
was utilized for anonymous interactivity between the audience and speakers during Q and A sessions. There were 324 text messages;
76 of these were either follow-up statements or questions related to earlier text messages. Forty-two questions were submitted
via paper notes. There was a general trend of an increasing number of text messages and a decreasing number of paper notes.
The anonymous text messaging system was found to be an effective method for interactivity between the audience and the speakers.
The questions and answers could be presented in a PowerPoint format at the formal Q and A sessions. Questions texted to the
authors during their talks could be immediately answered or addressed in subsequent talks. Although difficult for some individuals
to embrace technology, confexting allows for interactivity and prompts discussion. Confexting is an effective method for interactivity
between the audience and speakers not previously utilized in a conference setting. The anonymity and asynchronous communication
enable conference participants to submit more questions than in the traditional setting. The speakers may be able to explain
more thoroughly difficult concepts more thoroughly with additional slides at Q and A sessions or may immediately answer texted
questions during their talks. 相似文献
69.
Y Ando E Ando P I Ohlsson A Olofsson O Sandgren O Suhr H Terazaki K Obayashi E Lundgren M Ando A Negi 《Amyloid》1999,6(2):119-123
The aim of the present study was to analyze the forms of wild type and mutated monomeric transthyretin (Val30Met) in the amyloid fibrils of patients with familial amyloidotic polyneuropathy by electrospray ionization mass spectrometry (ESI-MS). The solubility of amyloid fibrils from the vitrectomized samples was examined to determine the appropriate solution for ESI-MS. ESI-MS analysis revealed that heterozygotic Val30Met amyloid fibrils contained 14.6 +/- 7.5% normal TTR. In all samples, 3 different types of variant ATTR could be identified: Full length ATTR, and -57, and -157 (or 156) Da from ATTR Val30Met were found. The two peaks showing -57, and -157 (or 156) Da from ATTR Val30Met corresponded to the -Gly, and -Gly-Pro sequences of ATTR Val30Met from the N-terminal. The results illustrate the heterogeneity of ATTR amyloid deposits and this method may be very useful for analyzing amyloid fibrils in ATTR related amyloidosis. 相似文献
70.
Antonio C Lerario Antonio R Chacra Augusto Pimazoni-Netto Domingos Malerbi Jorge L Gross José EP Oliveira Marilia B Gomes Raul D Santos Reine MC Fonseca Roberto Betti Roberto Raduan 《Diabetology & metabolic syndrome》2010,2(1):35
The Brazilian Diabetes Society is starting an innovative project of quantitative assessment of medical arguments of and implementing a new way of elaborating SBD Position Statements. The final aim of this particular project is to propose a new Brazilian algorithm for the treatment of type 2 diabetes, based on the opinions of endocrinologists surveyed from a poll conducted on the Brazilian Diabetes Society website regarding the latest algorithm proposed by American Diabetes Association /European Association for the Study of Diabetes, published in January 2009.An additional source used, as a basis for the new algorithm, was to assess the acceptability of controversial arguments published in international literature, through a panel of renowned Brazilian specialists. Thirty controversial arguments in diabetes have been selected with their respective references, where each argument was assessed and scored according to its acceptability level and personal conviction of each member of the evaluation panel.This methodology was adapted using a similar approach to the one adopted in the recent position statement by the American College of Cardiology on coronary revascularization, of which not only cardiologists took part, but also specialists of other related areas. 相似文献