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Vishnevskii EL Vishnevskii AE Shabel'nikova EI Safronova ON Dreval AA Topchieva GV Sukhorukov VS 《Bulletin of experimental biology and medicine》2004,138(5):440-441
Cytochemical activity of succinate dehydrogenase and lactate dehydrogenase in blood lymphocytes, activity of hydroperoxides, MDA, and blood plasma antioxidants were studied in experimental animals during immobilization stress after preliminary injection of doxazosin. Preliminary adrenergic receptor blockade prevented changes in cell metabolism characteristic of immobilization stress. The role of adrenergic regulation in the realization of energotropic effects during immobilization stress was examined.Translated from Byulleten Eksperimentalnoi Biologii i Meditsiny, Vol. 138, No. 11, pp. 496–498, November, 2004 相似文献
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Ciria HC Quevedo MS Cabrales LB Bruzón RP Salas MF Pena OG González TR López DS Flores JM 《BMC cancer》2004,4(1):1-10
Background
Tumor-associated antigens recognized by humoral effectors of the immune system are a very attractive target for human cancer diagnostics and therapy. Recent advances in molecular techniques have led to molecular definition of immunogenic tumor proteins based on their reactivity with autologous patient sera (SEREX).Methods
Several high complexity phage-displayed cDNA libraries from breast carcinomas, human testis and breast carcinoma cell lines MCF-7, MDA-MB-468 were constructed. The cDNAs were expressed in the libraries as fusion to bacteriophage lambda protein D. Lambda-displayed libraries were efficiently screened with sera from patients with breast cancer.Results
A panel of 21 clones representing 18 different antigens, including eight proteins of unknown function, was identified. Three of these antigens (T7-1, T11-3 and T11-9) were found to be overexpressed in tumors as compared to normal breast. A serological analysis of the 21 different antigens revealed a strong cancer-related profile for at least five clones (T6-2, T6-7, T7-1, T9-21 and T9-27).Conclusions
Preliminary results indicate that patient serum reactivity against five of the antigens is associated with tumor disease. The novel T7-1 antigen, which is overexpressed in breast tumors and recognized specifically by breast cancer patient sera, is potentially useful in cancer diagnosis. 相似文献99.
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