全文获取类型
收费全文 | 864篇 |
免费 | 88篇 |
国内免费 | 1篇 |
专业分类
耳鼻咽喉 | 6篇 |
儿科学 | 12篇 |
妇产科学 | 20篇 |
基础医学 | 132篇 |
口腔科学 | 6篇 |
临床医学 | 85篇 |
内科学 | 230篇 |
皮肤病学 | 7篇 |
神经病学 | 57篇 |
特种医学 | 5篇 |
外科学 | 195篇 |
综合类 | 3篇 |
预防医学 | 46篇 |
眼科学 | 17篇 |
药学 | 50篇 |
肿瘤学 | 82篇 |
出版年
2023年 | 3篇 |
2022年 | 17篇 |
2021年 | 37篇 |
2020年 | 17篇 |
2019年 | 27篇 |
2018年 | 37篇 |
2017年 | 25篇 |
2016年 | 30篇 |
2015年 | 27篇 |
2014年 | 36篇 |
2013年 | 55篇 |
2012年 | 64篇 |
2011年 | 72篇 |
2010年 | 29篇 |
2009年 | 31篇 |
2008年 | 53篇 |
2007年 | 50篇 |
2006年 | 57篇 |
2005年 | 46篇 |
2004年 | 39篇 |
2003年 | 37篇 |
2002年 | 42篇 |
2001年 | 7篇 |
2000年 | 5篇 |
1999年 | 3篇 |
1998年 | 8篇 |
1995年 | 2篇 |
1993年 | 3篇 |
1992年 | 5篇 |
1991年 | 7篇 |
1990年 | 5篇 |
1989年 | 9篇 |
1988年 | 5篇 |
1987年 | 7篇 |
1986年 | 5篇 |
1985年 | 6篇 |
1983年 | 3篇 |
1982年 | 3篇 |
1980年 | 2篇 |
1979年 | 4篇 |
1977年 | 5篇 |
1976年 | 2篇 |
1973年 | 2篇 |
1967年 | 2篇 |
1966年 | 4篇 |
1965年 | 2篇 |
1964年 | 2篇 |
1961年 | 2篇 |
1960年 | 1篇 |
1956年 | 1篇 |
排序方式: 共有953条查询结果,搜索用时 26 毫秒
81.
Sacha Gnjatic Brigitte Bressac-De Paillerets Jean-Grard Guillet Jeannine Choppin 《European journal of immunology》1995,25(6):1638-1642
In many cancer cells, the p53 gene displays point mutations that result in stabilization and accumulation of the p53 protein. Therefore, p53 peptides could be presented to the immune system by tumor cells; thus, p53 might be a suitable target antigen for developing an immunotherapy against tumors using cytotoxic T lymphocytes (CTL). To map candidate CTL epitopes, we synthesized 150 peptides of 8–11 residues that contained putative anchor motifs required for binding to common HLA class I molecules. They were tested for their capacity to promote the assembly of purified and refolded HLA-A1, A2, B7 and B8 molecules. The following wild-type p53 peptides were found to be reactive with the HLA molecules tested: 196–205 and 226–234 bound moderately to HLA-A1; 25–35, 65–73, 129–137, 187–197, 263–272 and 264–272 bound strongly, and 187–195 and 256–264 moderately to HLA-A2; 26–35, 63–73, 189–197, 249–257 and 321–330 bound strongly to HLA-B7; and 135–143, 210–218 and 375–383 bound weakly to HLA-B8. We also analyzed the effects of p53 mutations occurring naturally in tumors on peptide/HLA assembly. We found substitutions that enhanced, diminished or had no effect on the peptide binding to HLA molecules. Polymorphism at position 72 mainly affected peptide/HLA-B7 binding, the proline allele P72 giving a less-reactive peptide (63–73) than the arginine allele R72. We have ranked potential p53 epitopes according to their reactivity for purified HLA molecules, allowing the selection of appropriate peptides and HLA molecules to attempt CTL induction in vitro. 相似文献
82.
Inflammation or pain during neonatal development can result in long-term structural and functional alterations of nociceptive pathways, ultimately altering pain perception in adulthood. We have developed a mouse model of neonatal colon irritation (NCI) to investigate the plasticity of pain processing within the viscerosensory system. Mouse pups received an intracolonic administration of 2% mustard oil (MO) on postnatal days 8 and 10. Distal colons were processed at subsequent timepoints for myeloperoxidase (MPO) activity and growth factor expression. Adult mice were assessed for visceral hypersensitivity by measuring the visceromotor response during colorectal distension. Dorsal root ganglion (DRG) neurons from adult mice were retrogradely labeled from the distal colon and calcium imaging was used to measure transient receptor potential vanilloid 1 (TRPV1) and ankyrin 1 (TRPA1) responses to acute application of capsaicin and MO, respectively. Despite the absence of inflammation (as indicated by MPO activity), neonatal exposure to intracolonic MO transiently maintained a higher expression level of growth factor messenger RNA (mRNA). Adult NCI mice displayed significant visceral hypersensitivity, as well as increased sensitivity to mechanical stimulation of the hindpaw, compared to control mice. The percentage of TRPA1-expressing colon afferents was significantly increased in NCI mice, however they displayed no increase in the percentage of TRPV1-immunopositive or capsaicin-sensitive colon DRG neurons. These results suggest that early neonatal colon injury results in a long-lasting visceral hypersensitivity, possibly driven by an early increase in growth factor expression and maintained by permanent changes in TRPA1 function. 相似文献
83.
84.
85.
86.
Jérôme Le Pavec Séverine Feuillet Olaf Mercier Pauline Pradère Gaëlle Dauriat Adrian Crutu Valentina Florea Laurent Savale Marilyne Levy Florent Laverdure François Stephan Dominique Fabre Mitilian Delphine David Boulate Sacha Mussot Sébastien Hascoët Damien Bonnet Marc Humbert Elie Fadel 《The Journal of heart and lung transplantation》2021,40(7):652-661
87.
Sacha L. Filia Amanda L. Baker Caroline T. Gurvich Robyn Richmond Terry J. Lewin Jayashri Kulkarni 《Psychiatry research》2014
While research has identified gender differences in characteristics and outcomes of smokers in the general population, no studies have examined this among smokers with psychosis. This study aimed to explore gender differences among 298 smokers with psychosis (schizophrenia, schizoaffective and bipolar affective disorder) participating in a smoking intervention study. Results revealed a general lack of gender differences on a range of variables for smokers with psychosis including reasons for smoking/quitting, readiness and motivation to quit, use of nicotine replacement therapy, and smoking outcomes including point prevalence or continuous abstinence, and there were no significant predictors of smoking reduction status according to gender at any of the follow-up time-points. The current study did find that female smokers with psychosis were significantly more likely than males to report that they smoked to prevent weight gain. Furthermore, the females reported significantly more reasons for quitting smoking and were more likely to be driven by extrinsic motivators to quit such as immediate reinforcement and social influence, compared to the male smokers with psychosis. Clinical implications include specifically focussing on weight issues and enhancing intrinsic motivation to quit smoking for female smokers with psychosis; and strengthening reasons for quitting among males with psychosis. 相似文献
88.
Kröger C Dillon SC Cameron AD Papenfort K Sivasankaran SK Hokamp K Chao Y Sittka A Hébrard M Händler K Colgan A Leekitcharoenphon P Langridge GC Lohan AJ Loftus B Lucchini S Ussery DW Dorman CJ Thomson NR Vogel J Hinton JC 《Proceedings of the National Academy of Sciences of the United States of America》2012,109(20):E1277-E1286
89.
Atanackovic D Matsuo M Ritter E Mazzara G Ritter G Jäger E Knuth A Old LJ Gnjatic S 《Journal of immunological methods》2003,278(1-2):57-66
CD4+ T cells play an important role in the induction and maintenance of an effective antiviral and antitumor immune response. However, standardized monitoring of antigen-specific CD4+ T cells has not been established at the single-cell level. We now present a sensitive, specific, and simple methodology in which purified memory CD4+ T cells are expanded from PBMC in a single cycle of antigen-driven stimulation and quantitatively assayed by interferon-gamma ELISPOT. Issues of nonspecific background in assays were resolved with the use of innovative target cells, autologous PHA-expanded CD4+ T cells (T-APC). Remarkably, T-APC could not only present peptide epitopes from model antigens NY-ESO-1 and influenza nucleoprotein, but could also process full-length antigen endogenously expressed from recombinant fowlpox vector. This approach makes it possible to monitor CD4+ T cells in large series of patients, regardless of HLA haplotype, against the full peptide repertoire of a given antigen. 相似文献
90.