Nitric oxide interactions with cobalamins: biochemical and functional consequences

Nitric oxide (NO) is a paramagnetic gas that has been implicated in a wide range of biologic functions. The common pathway to evoke the functional response frequently involves the formation of an iron- nitrosyl complex in a target (heme) protein. In this study, we report on the interactions between NO and cobalt-containing vitamin B12 derivatives. Absorption spectroscopy showed that of the four Co(III) derivatives (cyanocobalamin [CN-Cbl], aquocobalamin [H2O-Cbl], adenosylcobalamin [Ado-Cbl], and methylcobalamin [MeCbl]), only the H2O- Cbl combined with NO. In addition, electron paramagnetic resonance spectroscopy of H2O-Cbl preparations showed the presence of a small amount of Cob-(II)alamin that was capable of combining with NO. The Co(III)-NO complex was very stable, but could transfer its NO moiety to hemoglobin (Hb). The transfer was accompanied by a reduction of the Co(III) to Co(II), indicating that NO+ (nitrosonium) was the leaving group. In accordance with this, the NO did not combine with the Hb Fe(II)-heme, but most likely with the Hb cysteine-thiolate. Similarly, the Co(III)-NO complex was capable of transferring its NO to glutathione. Ado-Cbl and Me-Cbl were susceptible to photolysis, but CN- Cbl and H2O-Cbl were not. The homolytic cleavage of the Co(III)-Ado or Co(III)-Me bond resulted in the reduction of the metal. When photolysis was performed in the presence of NO, formation of NO-Co(II) was observed. Co(II)-nitrosyl oxidized slowly to form Co(III)-nitrosyl. The capability of aquocobalamin to combine with NO had functional consequences. We found that nitrosylcobalamin had diminished ability to serve as a cofactor for the enzyme methionine synthase, and that aquocobalamin could quench NO-mediated inhibition of cell proliferation. Our in vitro studies therefore suggest that interactions between NO and cobalamins may have important consequences in vivo.     

薄层色谱溶剂系统的最优化方法

本文以模糊聚类分析法对81种常用溶剂进行分类,并根据最相邻原理从中优选出二十种色谱分析常用溶剂。再据此结果,以均匀设计试验法和最优化方法——单纯形法为手段,进行溶剂系统的最优化设计。并提出一个改良的色谱优化函数。经初步验证,这是一种比较有效与实用的系统化方法,尚未见国内外报道。本法不仅适用于薄层色谱,也适用于纸色谱及柱型液相色谱。     

Spontaneous bacterial peritonitis in fulminant hepatic failure

Ascites may be associated with fulminant hepatic failure (FHF), but spontaneous bacterial peritonitis (SBP) is an extremely rare complication. We report on two patients with FHF who developed SBP. One patient died and the other recovered.     

In vitro and in vivo selectin-blocking activities of sulfated lipids and sulfated sialyl compounds

There is accumulating evidence that sulfated lipids, sulfated oligosaccharides and other sulfated compounds are reactive with selectins in a manner that interferes with selectin interactions with their natural ligands. In the report we describe the ability of sulfated lipids (sulfatides and gangliosides) and multimeric forms of sulfated sialic acid to block binding of P- and E-selectin-Ig to neutrophils. The in vivo ability of these compounds to block lung injury in rats following i.v. infusion of purified cobra venom factor (CVF), which induces injury that is L- and P-selectin dependent, was also determined as well as effects on recruitment of neutrophils, as measured by lung myeloperoxidase. There was a significant correlation between the ability of sulfated lipids and sialyl compounds to interfere in vitro with P-selectin-Ig binding to neutrophils and to protect against P-selectin-dependent acute lung injury induced by CVF. The biological effects of these sulfated compounds were also associated with diminished accumulation of neutrophils. The protective effects of these compounds may be linked to their ability to interfere with P- selectin binding to counter-receptors on neutrophils.      

诺氟沙星的吸附行为及其测定

诺氟沙星在0.05mol·L^-1NH₄Cl (pH 5.8)溶液中,出现一灵敏的示波极谱还原峰。峰电位为-1.44 V (vs SCE)。峰高与诺氟沙星的浓度在1.0×10^-7～4.0×10^-6mol·L^-1范围内成正比,检出限为5.0×10^-8mol·L^-1,并用于样品的测定。用线性扫描与循环伏安法等手段研究体系的吸附性质。实验表明,该电极过程为具有吸附性的不可逆过程。     

Kalman滤波分光光度法同时测定多相脂质体口服液中氟尿嘧啶和尼泊金的含量

本文提出了对测量值平滑后,用Kalman滤波分光光度法同时测定多相脂质体口服液中氟尿嘧啶和尼泊金的含量,改善了滤波效果,较好地解决了复杂基质存在下多组分的同时测定。用人工合成样考察表明,在269～273nm间滤波结果较好。用分别相当制剂标示量80,100,120%的两组分九种可能组合人工合成样品考查结果表明,氟尿嘧啶和尼泊金的回收率分别为99.3～102.6%和97.8～103.0%;当存在系统误差时,回收率分别为99.4～102.7%和97.0～102.9%