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11.
Erika Rodrigues-Silva Edilene S. Siqueira-Santos Juliana S. Ruas Raffaela S. Ignarro Tiago R. Figueira Fábio Rogério Roger F. Castilho 《Journal of neuro-oncology》2017,133(3):519-529
High-grade gliomas are aggressive and intensely glycolytic tumors. In the present study, we evaluated the mitochondrial respiratory function of glioma cells (T98G and U-87MG) and fresh human glioblastoma (GBM) tissue. To this end, measurements of oxygen consumption rate (OCR) were performed under various experimental conditions. The OCR of T98G and U-87MG cells was well coupled to ADP phosphorylation based on the ratio of ATP produced per oxygen consumed of ~2.5. In agreement, the basal OCR of GBM tissue was also partially associated with ADP phosphorylation. The basal respiration of intact T98G and U-87MG cells was not limited by the supply of endogenous substrates, as indicated by the increased OCR in response to a protonophore. These cells also displayed a high affinity for oxygen, as evidenced by the values of the partial pressure of oxygen when respiration is half maximal (p 50). In permeabilized glioma cells, ADP-stimulated OCR was only approximately 50% of that obtained in the presence of protonophore, revealing a significant limitation in oxidative phosphorylation (OXPHOS) relative to the activity of the electron transport system (ETS). This characteristic was maintained when the cells were grown under low glucose conditions. Flux control coefficient analyses demonstrated that the impaired OXPHOS was associated with the function of both mitochondrial ATP synthase and the adenine nucleotide translocator, but not the phosphate carrier. Altogether, these data indicate that the availability and metabolism of respiratory substrates and mitochondrial ETS are preserved in T98G and U-87MG glioma cells even though these cells possess a relatively restrained OXPHOS capability. 相似文献
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Oxidative stress biomarkers of exposure in the blood of cichlid species from a metal-contaminated river 总被引:1,自引:0,他引:1
Ruas CB Carvalho Cdos S de Araújo HS Espíndola EL Fernandes MN 《Ecotoxicology and environmental safety》2008,71(1):86-93
The oxidative stress biomarkers of exposure, such as reduced glutathione (GSH), activity of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx) and the levels of lipid peroxidation (LPO), were measured in the blood of three cichlid fish (Oreochromis niloticus, Tilapia rendalli, and Geophagus brasiliensis) taken during two seasons from two sites, unpolluted and polluted by industrial effluents, to evaluate the effectiveness of these biomarkers in assessing the impact of water contamination. The LPO levels in the blood were higher in fish from the metal-contaminated site and the chronic exposure led to significant changes in GPx, CAT, and SOD activities in all three cichlid species. The considerable variation of responses in these cichlids to water contamination evidenced differences in sensitivity to the metal contamination and/or in the potential to respond to it highlighting the importance of using a set of related biomarkers to assess the impact of water contamination. 相似文献
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Vânia Rodrigues Leite e Silva MSc Marcelo Ariel Schulman Cibeli Ferelli Janine Mailho Gimenis Gabriele Wander Ruas ré Rolim Baby PhD Maria Valéria Robles Velasco PhD Maria Elena Taqueda PhD & Telma Mary Kaneko PhD 《Journal of Cosmetic Dermatology》2009,8(1):32-39
Background Water is an important factor on the appearance and function of the skin and, when dehydrated, it becomes rough and flaky. The measurement of stratum corneum hydration is widely employed to verify the moisture effect of topical products.
Aims This study has evaluated in vivo the stratum corneum hydration, by the electrical measurements of skin, after treatment with different moisturizers presented in gel base. MoistureMeter® and Corneometer were used as bio-instruments. Urea, the herbal extract ( Imperata cylindrical ), the NMF™ components and the carbohydrate derivate compound (xylityglucoside, anhydroxylitol, and xylitol) were used as the active substances.
Methods The study protocol was carried out according to the three-factor factorial design. The gels were applied on both forearms of eight female volunteers. However, each volunteer had one untreated skin area as the skin moisture control. The electrical capacitance of the skin was measured by both instruments in different times: after application (0 time), 30, 60, 120, 240 and 360 min.
Results Gel base, gel containing herbal extract, and gel containing NMF™ components statistically have equal moisture effect measurements, according to both bio-instruments. However, the values obtained for urea, carbohydrate derivate compound, and untreated skin (control) have statically different results in both devices.
Conclusion The gel containing urea and the carbohydrate derivate compound gel have promoted the most intense moisture effect compared with the other formulations. The measurements of hydration between the devices generated different absolute values; however, the statistical analysis has shown similar precision among the measurements. 相似文献
Aims This study has evaluated in vivo the stratum corneum hydration, by the electrical measurements of skin, after treatment with different moisturizers presented in gel base. MoistureMeter® and Corneometer were used as bio-instruments. Urea, the herbal extract ( Imperata cylindrical ), the NMF™ components and the carbohydrate derivate compound (xylityglucoside, anhydroxylitol, and xylitol) were used as the active substances.
Methods The study protocol was carried out according to the three-factor factorial design. The gels were applied on both forearms of eight female volunteers. However, each volunteer had one untreated skin area as the skin moisture control. The electrical capacitance of the skin was measured by both instruments in different times: after application (0 time), 30, 60, 120, 240 and 360 min.
Results Gel base, gel containing herbal extract, and gel containing NMF™ components statistically have equal moisture effect measurements, according to both bio-instruments. However, the values obtained for urea, carbohydrate derivate compound, and untreated skin (control) have statically different results in both devices.
Conclusion The gel containing urea and the carbohydrate derivate compound gel have promoted the most intense moisture effect compared with the other formulations. The measurements of hydration between the devices generated different absolute values; however, the statistical analysis has shown similar precision among the measurements. 相似文献
14.
Dr. M. C. Mota E. Leite M. A. Ruas Dr. H. L. Verjans C. B. Blakemore Prof.Dr. J. G. Cunha-Vaz 《International ophthalmology》1987,10(1):3-9
A randomized, double-blind, placebo controlled study to investigate the long-term effect of CyclospasmolR (cyclandelate) on the abnormal permeability of the blood-retinal barrier was performed in 26 patients with insulin-dependent diabetes mellitus for at least 1 year and minimal retinopathy. Cyclospasmol 400 mg or placebo capsules were taken 4 times daily for 12 months by equal numbers in both groups.Each patient underwent a routine ophthalmoscopic examination, retinal fluorescein angiography and quantitative vitreous fluorophotometry to assess the permeability of the blood-retinal barrier just before the trial and following 6 and 12 months of therapy. Laboratory tests for determining blood and urine glucose levels and blood HbA1-levels were also carried out at these assessments.Statistically significant changes in diabetic control, in HbA1-levels or in the frequency of retinal microaneurysms could not be shown in either treatment group during the trial, nor were there any significant differences in these parameters between the two groups. Analysis of fluorophotometric data on fluorescein penetration into the left posterior vitreous demonstrated significant reductions in this parameter during the trial compared to the pretreatment level in Cyclospasmol treated diabetics. These changes in the pretreatment level after 6 and 12 months also differed significantly between the two groups.However, this statistically significant beneficial reduction in fluorescein penetration into the left posterior vitreous did not occur in the right eye in the Cyclospasmol group.In placebo treated patients a consistently deleterious trend for this parameter was observed for both eyes during the one year study. The consistently beneficial trend in the right eye following Cyclospasmol and the consistently deleterious trend in both eyes following placebo with regard to the abnormal permeability of the blood-retinal barrier of diabetics with minimal retinopathy could probably be explained by the small number of patients in both groups. No side-effects were reported. 相似文献
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Laura M. J. de F. Santos Maria Cecília F. Damé Beatris G. Cademartori Nilton A. da Cunha Filho Nara Amélia da R. Farias Jerônimo L. Ruas 《Acta parasitologica / Witold Stefański Institute of Parasitology, Warszawa, Poland》2013,58(3):334-336
Serum samples from 169 water buffaloes and 121 beef cattle were analyzed for antibodies to T. gondii by an indirect fluorescent antibody test (IFAT). Positive results were obtained in 27.2% of water buffaloes and 17.4% of cattle. Statistical analysis indicated significant differences between the prevalence in cattle and buffalo (p ≤ 0.05). The highest titres found in positive animals were 1:256 (buffaloes) and 1:64 (cattle). In both bovine species, toxoplasmosis frequency in young animals (less than 2 years old) was lower compared to older individuals, although the differences seen in cattle were not statistically significant. 相似文献
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Thaís dos Santos Fontes Pereira DDS MSc Fernanda Cristina Melo Pelinsari DDS Bruna Mara Ruas DDS Luanne Priscila Pereira Avelar DDS Vitor José da Fonseca DDS Mauro Henrique Nogueira de Abreu DDS PhD Ulisses Eliezer Salomão DDS MSc Agnaldo Soares Lima MD PhD Maria Elisa de Souza e Silva DDS PhD Ricardo Santiago Gomez DDS PhD 《Special care in dentistry》2016,36(5):277-281