The inhibitory effects of Radix Astragali on hypoxic pulmonary hypertension of rats

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神经节苷脂GM1对阿尔采默病β-淀粉样蛋白前体代谢的影响

目的研究外源性神经节苷脂ＧＭ１干扰细胞β－淀粉样蛋白前体代谢的作用,探讨ＧＭ１与阿尔采默病特征性病理变化老年斑和其他淀粉样病变形成的关系。方法选用人类β－淀粉样蛋白前体基因（ＡＰＰ６９５）转染细胞模型,用同位素代谢标记方法测定ＧＭ１对细胞β－淀粉样蛋白前体代谢及其水解产物分泌性β－淀粉样蛋白前体释放的影响;用Ｗｅｓｔｅｒｎ印迹和斑点印迹法分析ＧＭ１与重组β－淀粉样蛋白前体的亲和性。结果ＧＭ１干扰细胞β－淀粉样蛋白前体的水解代谢和抑制分泌性β－淀粉样蛋白前体的释放,ＧＭ１与β－淀粉样蛋白前体特异性结合。结论ＧＭ１代谢异常可能与阿尔采默病脑淀粉样蛋白聚积和沉淀有关。     

Expression of nm23 gene in hepatocellular carcinoma tissue and its relation with metastasis

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Ultrastructure of muscle in fetal Duchenne's dystrophy

Skeletal muscle was studied from two male fetuses with Duchenne's muscular dystrophy (DMD) that were at 17 and 23 weeks' gestational age, respectively. In comparison with the ultrastructure of normal fetal muscle, a spectrum of changes could be seen, from normal-appearing to very degenerated fibers. The changes followed an apparent progression from early actin filament disruption and fraying of the Z bands to progressive disorganization of myofibrillar alignment, increasing vacuolization, and condensation of fibers. Significant plasma membrane defects were not found. These findings are similar to those described in older persons with DMD and confirm that DMD is a congenital disorder associated with consistent skeletal muscle changes in utero.     

上球管遥控床的智能控制

介绍能隔室遥控操作的上球管遥控床的智能控制系统,包括用VB编制的上位机监控界面,基于AT90S8515的下位机的控制,以及上、下位机的串行通信.本系统具有友好的人机界面,性能良好,易于产品的升级、改进.     

二氢叶酸还原酶和谷氨酰胺合成酶双基因筛选扩增系统对外源基因表达的影响

目的 研究二氢叶酸还原酶（DHFR，D）与谷氨酰胺合成酶（GC，G）单基因和二氢叶酸还原酶与谷氨酰胺合成酶（DHFR GS，DG）双基因筛选扩增系统对外源基因表达的影响。方法 选择N端部分TPO基因（T184）作为靶基因，以DHFR和GS基因作为筛选及扩增基因构建重组质粒pDCT184与pGCT184，将两种质粒分别转染CHOdhfr^-细胞形成单基因筛选扩增系统，两种质粒共转染CHOdhfr^-细胞形成双基因筛选扩增系统，在双基因筛选扩增系统中设计三种药物加压方式，第一种为DG方式；先用DHFR系统压力（MTX）筛选，再用GS系统压力（MSX）筛选；第二种为GD方式；先用GS系统压力筛选（MSX），再用DHFR系统压力筛选（MTX）；第三种为共加压方式；同时利用DHFR与GS系统压力筛选（MTX MSX）。通过比较T184基因拷贝数及表达水平来研究DHFR和GS双基因筛选扩增系统对外源基因表达的影响。结果 T184基因拷贝数及表达水平在DG双基因选择方式没有明显差异。结论 采用DHFR GS双基因筛选扩增系统共加压方式是提高CHOdhfr^-细胞表达外源基因的有效途径。     

Comparison of methods for measuring longitudinal brain change in cognitive impairment and dementia

PURPOSE: The goal of this project was to compare MRI measures of hippocampal, entorhinal cortex (ERC), and whole brain longitudinal change in cognitively normal elderly controls (C), non-demented subjects with cognitive impairment (CI), and demented (D) subjects. METHODS: 16 C, 6 CI, and 7 D subjects of comparable age were studied with MRI twice, at least 1 year apart. Longitudinal change in total brain size was measured by several methods, including computerized segmentation, non-linear warping, and change in the fluid/tissue boundaries between cerebrospinal fluid (CSF) and brain. Change in hippocampal volume was measured by semi-automated methods, and ERC volumes were manually measured. RESULTS: The annual rate of atrophy was greater in D versus C and D versus CI for cortical gray matter (cGM) (P=0.009 and 0.002), hippocampus (P=0.0001 and 0.002), and for the change in the fluid/tissue boundary (P=0.03 and 0.03). The annual rate of atrophy of ERC was greater in both CI and D versus C (P=0.01 and 0.0002). No significant differences between groups were found using non-linear warping. CONCLUSIONS: In CI, the greatest annual rates of atrophy were in ERC, while in D the greatest annual rates of atrophy were in hippocampus and cortex. Progressive ERC atrophy was observed with a greater degree of cognitive impairment, while hippocampal and cortical atrophy were only observed in demented subjects.     

Edwards套棒方法治疗胸腰段脊柱不稳定损伤的生物力学实验研究

本文报告Edwards套棒装置及单纯Harrington分离棒治疗胸腰椎不稳定损伤的力学实验研究结果。取成年猪新鲜胸腰椎脊柱6具,切断前纵韧带、椎间盘纤维环、后纵韧带,造成前中柱不稳定损伤15处。测力传感器固定于椎体前缘并骑跨损伤处,使之检测纵向复位力。单侧内固定装置固定。实验结果表明:单侧Edwards套棒装置产生纵向复位力量89N,较Harrington分离棒48.7N提高82.7％(P<0.0005);二种内固定装置分别用于胸、腰椎时,纵向复位力均无显著差异(P<0.05),表明胸段肋骨对纵向复位力无影响。生物力学分析,纵向复位力与横向前推力成正比例关系,增大内固定装置对伤椎的横向前推力,可提高纵向复位力量。同时其它学者也报告Edwards套棒装置具有良好的脊柱三维稳定作用。所以,作者认为Edwards套棒装置集对脊柱骨折复位与稳定于一体,是理想的内固定装置。     

老年大鼠蓝斑胶质细胞超微结构的变化

本实验以青年鼠为对照,观察老年大鼠蓝班内胶质细胞超微结构的改变.结果显示:老年大鼠蓝斑内出现许多肥大星形胶质细胞,这些细胞胞浆内含有丰富的核糖体、粗面内质网和线粒体.少突胶质细胞增生,胞浆含有大量细胞器、突起内含有变性的轴突、终末和树突.小胶质细胞也增生,其胞浆内出现大量脂滴、脂褐素.在一些较大的腔隙内,可观察到巨噬细胞.与此同时,退变的星形胶质细胞亦可见到,这些细胞浆内出现大量致密体、变性的线粒体、胶质原纤维和糖元颗粒.上述结果提示:在脑衰老过程中,胶质细胞的形态和数量已发生了一系列变化,这些变化一方面有利于修复变性的神经元和清除坏死的神经元,另一方面也提示星形胶质细胞本身面临着退变和死亡.