全文获取类型
收费全文 | 1292篇 |
免费 | 59篇 |
国内免费 | 2篇 |
专业分类
耳鼻咽喉 | 11篇 |
儿科学 | 42篇 |
妇产科学 | 15篇 |
基础医学 | 139篇 |
口腔科学 | 16篇 |
临床医学 | 100篇 |
内科学 | 237篇 |
皮肤病学 | 26篇 |
神经病学 | 261篇 |
特种医学 | 45篇 |
外科学 | 227篇 |
综合类 | 10篇 |
预防医学 | 89篇 |
眼科学 | 9篇 |
药学 | 85篇 |
中国医学 | 1篇 |
肿瘤学 | 40篇 |
出版年
2023年 | 7篇 |
2022年 | 17篇 |
2021年 | 25篇 |
2020年 | 16篇 |
2019年 | 32篇 |
2018年 | 35篇 |
2017年 | 27篇 |
2016年 | 33篇 |
2015年 | 31篇 |
2014年 | 37篇 |
2013年 | 35篇 |
2012年 | 86篇 |
2011年 | 81篇 |
2010年 | 46篇 |
2009年 | 40篇 |
2008年 | 54篇 |
2007年 | 54篇 |
2006年 | 66篇 |
2005年 | 55篇 |
2004年 | 49篇 |
2003年 | 57篇 |
2002年 | 68篇 |
2001年 | 37篇 |
2000年 | 54篇 |
1999年 | 44篇 |
1998年 | 17篇 |
1997年 | 14篇 |
1996年 | 7篇 |
1995年 | 8篇 |
1994年 | 9篇 |
1993年 | 10篇 |
1992年 | 21篇 |
1991年 | 22篇 |
1990年 | 20篇 |
1989年 | 21篇 |
1988年 | 16篇 |
1987年 | 15篇 |
1986年 | 11篇 |
1985年 | 16篇 |
1984年 | 5篇 |
1983年 | 3篇 |
1982年 | 5篇 |
1981年 | 3篇 |
1980年 | 3篇 |
1979年 | 5篇 |
1978年 | 7篇 |
1976年 | 6篇 |
1975年 | 3篇 |
1974年 | 3篇 |
1933年 | 2篇 |
排序方式: 共有1353条查询结果,搜索用时 31 毫秒
41.
42.
43.
Transneuronal tracing of diverse CNS circuits by Cre-mediated induction of wheat germ agglutinin in transgenic mice 下载免费PDF全文
Braz JM Rico B Basbaum AI 《Proceedings of the National Academy of Sciences of the United States of America》2002,99(23):15148-15153
Systems neuroscience addresses the complex circuits made by populations of neurons in the CNS and the cooperative function of these neurons. Improved approaches to the neuroanatomical analysis of CNS circuits are thus of great interest. In fact, significant advances in tract-tracing methods have recently been made by using transgenic mice that express transneuronal lectin tracers under the control of neuron-specific promoters. The utility of those animals, however, is limited to the CNS circuit influenced by the particular promoter. Here, we describe a new transgenic mouse that can be used for transneuronal tracing analysis of circuits in any region of the brain or spinal cord. The transgene in these mice results in expression of LacZ in neurons throughout the CNS. Excision of the LacZ gene by Cre-mediated recombination initiates expression of the lectin, wheat germ agglutinin (WGA). To illustrate the diverse uses of these ZW (LacZ-WGA) mice, we triggered WGA expression either by crossing the mice with two Cre-expressing transgenic mouse lines or by microinjecting a Cre-expressing adeno-associated virus into the cerebellum or cerebral cortex. Both approaches resulted in extensive WGA expression in the cell bodies and dendrites of neurons in which the recombination event occurred, as well as anterograde and transneuronal transport of the lectin to second and third order neurons. Because the lectin can be induced in developing and adult animals, and in all regions of the brain and spinal cord, these ZW may prove extremely valuable for numerous studies of CNS circuit analysis. 相似文献
44.
45.
46.
Biopsy‐proven acute cellular rejection as an efficacy endpoint of randomized trials in liver transplantation: a systematic review and critical appraisal 下载免费PDF全文
Manuel Rodríguez‐Perálvarez Jose M. Rico‐Juri Emmanuel Tsochatzis Patrizia Burra Manuel De la Mata Jan Lerut 《Transplant international》2016,29(9):961-973
Biopsy‐proven acute cellular rejection (ACR) is the primary efficacy endpoint in most randomized trials evaluating immunosuppression in liver transplantation. However, ACR is not a major cause of graft loss, and a certain grade of immune activation may be even beneficial for long‐term graft acceptance. Validated criteria to select candidates for liver biopsy are lacking, and routine clinical practice relies on liver tests, which are inaccurate markers of ACR. Indeed, both the agreement among clinicians to select candidates for liver biopsy and the correlation between the clinical suspicion of ACR and histological findings are poor. In randomized trials evaluating immunosuppression protocols, this concern grows exponentially due to the open‐label and multicenter nature of most studies. Therefore, biopsy‐proven ACR is a suboptimal efficacy endpoint given its limited impact on prognosis and the heterogeneous diagnosis, which may increase the risk of bias. Chronic rejection and/or graft loss would be more appropriate endpoints, but would certainly require larger studies with prolonged surveillances. An objective method to select candidates for liver biopsy is therefore urgently needed, and only severe episodes of histological ACR should be considered as potentially harmful. Emerging surrogate markers of ACR and antibody‐mediated rejection require further investigation to determine their clinical role. 相似文献
47.
Risk Factors Associated With Early Invasive Pulmonary Aspergillosis in Kidney Transplant Recipients: Results From a Multinational Matched Case–Control Study 下载免费PDF全文
F. Lpez‐Medrano J. T. Silva M. Fernndez‐Ruiz P. L. Carver C. van Delden E. Merino M. J. Prez‐Saez M. Montero J. Coussement M. de Abreu Mazzolin C. Cervera L. Santos N. Sab A. Scemla E. Cordero L. Cruzado‐Vega P. L. Martín‐Moreno
. Len E. Rudas A. Ponce de Len M. Arriola R. Lauzurica M. David C. Gonzlez‐Rico F. Henríquez‐Palop J. Fortún M. Nucci O. Manuel J. R. Pao‐Pardo M. Montejo P. Muoz B. Snchez‐Sobrino A. Mazuecos J. Pascual J. P. Horcajada T. Lecompte C. Lumbreras A. Moreno J. Carratal M. Blanes D. Hernndez E. A. Hernndez‐Mndez M. C. Farias M. Perell‐Carrascosa J. M. Morales A. Andrs J. M. Aguado 《American journal of transplantation》2016,16(7):2148-2157
Risk factors for invasive pulmonary aspergillosis (IPA) after kidney transplantation have been poorly explored. We performed a multinational case–control study that included 51 kidney transplant (KT) recipients diagnosed with early (first 180 posttransplant days) IPA at 19 institutions between 2000 and 2013. Control recipients were matched (1:1 ratio) by center and date of transplantation. Overall mortality among cases was 60.8%, and 25.0% of living recipients experienced graft loss. Pretransplant diagnosis of chronic pulmonary obstructive disease (COPD; odds ratio [OR]: 9.96; 95% confidence interval [CI]: 1.09–90.58; p = 0.041) and delayed graft function (OR: 3.40; 95% CI: 1.08–10.73; p = 0.037) were identified as independent risk factors for IPA among those variables already available in the immediate peritransplant period. The development of bloodstream infection (OR: 18.76; 95% CI: 1.04–339.37; p = 0.047) and acute graft rejection (OR: 40.73, 95% CI: 3.63–456.98; p = 0.003) within the 3 mo prior to the diagnosis of IPA acted as risk factors during the subsequent period. In conclusion, pretransplant COPD, impaired graft function and the occurrence of serious posttransplant infections may be useful to identify KT recipients at the highest risk of early IPA. Future studies should explore the potential benefit of antimold prophylaxis in this group. 相似文献
48.
Deving?Arias RamosView authors OrcID profile John?Alexander?AlzateView authors OrcID profile Jhon?Alejandro?Rico Gallego Natalia?Acevedo Escalante 《BMC infectious diseases》2018,18(1):704
Background
Those infected by human immunodeficiency virus (HIV) have a higher risk of opportunistic infections. The risk is related to the level of immunosuppression. We report a case of a young male with the unusual scenario of three opportunistic infections occurring simultaneously: Cryptococcosis, Histoplasmosis and Cryptosporidiosis. Histoplasmosis and cryptococcosis are major causes of morbimortality in immunocompromised patients due to HIV infection.Case presentation
We report the case of a patient with HIV infection with a CD4 T lymphocyte cell (CD4) count of 2 cells/mm3, who presented with 6?months of diarrhea, non-productive dry cough, nocturnal diaphoresis, fever, weight loss, and a maculopapular rash. He had a concurrent infection with three opportunistic microorganisms: fungemia by cryptococcosis, disseminated histoplasmosis confirmed by detection of the antigen in urine and chronic diarrhea by cryptosporidiosis confirmed by direct observation in feces by modified Ziehl–Neelsen stain. The patient received antifungal treatment with a satisfactory outcome.Conclusions
There are still regions where HIV detection programs are deficient thus facilitating occurrence of HIV infection cases in advanced stages of immunosuppression. A high level of suspicion of systemic mycoses and concurrent infection by several opportunistic pathogens is required in severely immunocompromised patients.49.
B Gandarias Maria D Alonso M Fernández Rivas L Fernández-Távora P Miranda D Mu?oz Lejarazu M Sotillo J Tejero P Rico 《Journal of investigational allergology & clinical immunology》2005,15(4):242-248
With the aim of evaluating tolerance to new shorter initiation schedules in subcutaneous immunotherapy everyday clinical practice, a study was carried out using Pangramin Plus with initiation periods between 3 (Cluster) and 6 (Plus) weeks. All the information was processed retrospectively and both systemic (SR) and local (LR) adverse reactions occurring between September 2002 and February 2003 were recorded. A total of 353 patients (261 Plus and 91 Cluster) were included and 2,886 doses were administered (2,166 in initiation and 720 in maintenance). Of these, 800 were with Grass mix extract, 1,141 Grass mix + Olea, 273 Olea, 73 Dermatophagoides mix and 599 Dermatophagoides pteronyssinus. As regards adverse reactions (AR), 2.8% of patients showed SR and 4.8% LR, 1.2% of doses caused some type of reaction (SR and LR in 0.3% and 0.9%, respectively). The initiation schedule, first dose or allergens resulted in no significant differences in the frequency of adverse reactions. The Grass mix extract showed the highest frequency of AR. Sixty-seven percent of SR and 68% of LR were delayed. 64% of these reactions resolved spontaneously while the rest responded favourably to treatment. Adrenaline was administered on one occasion for immediate asthma. There were no cases of anaphylactic shock, hospitalisation or life-threatening situations. Pangramin Plus tolerance, therefore, can be classified as good, similar to conventional schedules, but with the benefits of shorter initiation schedules. 相似文献
50.
Simone C. Oudshoorn Gabriel J. E. Rinkel Andrew J. Molyneux Richard S. Kerr Sanne M. Dorhout Mees Daan Backes Ale Algra Mervyn D. I. Vergouwen 《Neurocritical care》2014,21(1):4-13