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41.
Ca2+-dependent modulation of single human cardiac L-type calcium channels by the calcineurin inhibitor cyclosporine 总被引:1,自引:0,他引:1
Matthes J Jäger A Handrock R Groner F Mehlhorn U Schwinger RH Varadi G Schwartz A Herzig S 《Journal of molecular and cellular cardiology》2004,36(2):241-255
OBJECTIVE: Activity of single L-type calcium channels (LTCC) is enhanced in human failing myocardium (Circulation 98 (1998) 969.), most likely due to impaired dephosphorylation. Protein phosphatase 2B (calcineurin) has recently been shown to be involved in heart failure pathophysiology. We now focus on the regulation of single LTCC by calcineurin that were prevented by Ca(2+)-free experimental conditions in our previous study. METHODS: Single LTCC currents were recorded in myocytes from human atrium and ventricle. Charge carriers were 70 mM Ba(2+), or a mixture of 30 mM Ca(2+) and 60 mM Ba(2+) to facilitate Ca(2+) permeation through recorded channels. The calcineurin inhibitor cyclosporine (10 microM) was used to reveal a putative role for calcineurin in regulation of LTCC. RESULTS: A mixture of Ca(2+) and Ba(2+) as charge carriers allowed for Ca(2+) permeation through recombinant human embryonic kidney cells and native (atrial and ventricular) human cardiac LTCC. With only Ba(2+) as the charge carrier, activities of both ventricular and atrial LTCC were strongly decreased by cyclosporine. In contrast, channel activity remained constant when Ca(2+) permeation was provided. In the presence of thapsigargin and (S)-BayK 8644, cyclosporine here even increased channel activity. CONCLUSIONS: We propose a dual cyclosporine effect on human cardiac LTCC. A non-specific inhibitory effect prevails with Ba(2+) permeation but can be compensated or overcome by a specific Ca(2+)-dependent stimulation with Ca(2+) permeation. More complete restoration of physiological Ca(2+) movements (e.g., Ca(2+) release from sarcoplasmic reticulum) will help to define even more precisely the involvement of calcineurin in regulation of human cardiac LTCC. 相似文献
42.
Vergara C Tsai YJ Grant AV Rafaels N Gao L Hand T Stockton M Campbell M Mercado D Faruque M Dunston G Beaty TH Oliveira RR Ponte EV Cruz AA Carvalho E Araujo MI Watson H Schleimer RP Caraballo L Nickel RG Mathias RA Barnes KC 《American journal of respiratory and critical care medicine》2008,178(10):1017-1022
43.
Christoph Josef Spindelegger Konstantinos Papageorgiou Renate Grohmann Rolf Engel Waldemar Greil Anastasios Konstantinidis Marcus Willy Agelink Stefan Bleich Eckart Ruether Sermin Toto Siegfried Kasper 《The international journal of neuropsychopharmacology / official scientific journal of the Collegium Internationale Neuropsychopharmacologicum (CINP)》2015,18(4)
44.
Head-to-head comparison of B-type natriuretic peptide (BNP) and NT-proBNP in daily clinical practice
Mair J Gerda F Renate H Ulmer H Andrea G Pachinger O 《International journal of cardiology》2008,124(2):244-246
B-type natriuretic peptide (BNP; Abbott Diagnostics) and N-terminal proBNP (NT-proBNP, Roche Diagnostics) were compared in consecutive samples of 458 patients (mean age 60 years+/-16 years; 159 female, 299 male) sent for NT-proBNP measurement to investigate influences on both markers. BNP and NT-proBNP showed a close correlation with each other (r=0.89, p<0.0001). Using age- and gender-adjusted upper reference values the inter-rater agreement of both parameters was satisfactory (83%, Cohen's kappa coefficient=0.7). The combination of normal BNP and elevated NT-proBNP was significantly more frequent than vice versa (61 vs. 16 patients), and a calculated glomerular filtration rate<60 ml/min/1.73 m(2) was found in 39% of these patients. Multiple linear regression analysis revealed a significant influence of a reduced ejection fraction (<50%), renal dysfunction (calculated glomerular filtration rate<60 ml/min/1.73 m(2)), anemia, hypertension, age, and gender on both BNP and NT-proBNP. In conclusion, despite a close correlation and a satisfactory agreement between both markers in classification, frequent discrepancies in individual patients demonstrate that both markers are clinically not completely equivalent. 相似文献
45.
Development of a synthetic tissue engineered three‐dimensional printed bioceramic‐based bone graft with homogenously distributed osteoblasts and mineralizing bone matrix in vitro
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Renate Gildenhaar Georg Berger Cynthia Gomes Ulf Linow Martin Hardt Barbara Peleska Jens Günster Michael Stiller Alireza Houshmand Khaled Abdel Ghaffar Ahmed Gamal Mohamed El‐Mofty Christine Knabe 《Journal of tissue engineering and regenerative medicine》2018,12(1):44-58
Over the last decade there have been increasing efforts to develop three‐dimensional (3D) scaffolds for bone tissue engineering from bioactive ceramics with 3D printing emerging as a promising technology. The overall objective of the present study was to generate a tissue engineered synthetic bone graft with homogenously distributed osteoblasts and mineralizing bone matrix in vitro, thereby mimicking the advantageous properties of autogenous bone grafts and facilitating usage for reconstructing segmental discontinuity defects in vivo. To this end, 3D scaffolds were developed from a silica‐containing calcium alkali orthophosphate, using, first, a replica technique – the Schwartzwalder–Somers method – and, second, 3D printing, (i.e. rapid prototyping). The mechanical and physical scaffold properties and their potential to facilitate homogenous colonization by osteogenic cells and extracellular bone matrix formation throughout the porous scaffold architecture were examined. Osteoblastic cells were dynamically cultured for 7 days on both scaffold types with two different concentrations of 1.5 and 3 × 109 cells/l. The amount of cells and bone matrix formed and osteogenic marker expression were evaluated using hard tissue histology, immunohistochemical and histomorphometric analysis. 3D‐printed scaffolds (RPS) exhibited more micropores, greater compressive strength and silica release. RPS seeded with 3 × 109 cells/l displayed greatest cell and extracellular matrix formation, mineralization and osteocalcin expression. In conclusion, RPS displayed superior mechanical and biological properties and facilitated generating a tissue engineered synthetic bone graft in vitro, which mimics the advantageous properties of autogenous bone grafts, by containing homogenously distributed terminally differentiated osteoblasts and mineralizing bone matrix and therefore is suitable for subsequent in vivo implantation for regenerating segmental discontinuity bone defects. Copyright © 2016 John Wiley & Sons, Ltd. 相似文献
46.
47.
Diane Purper-Ouakil Hilario Blasco-Fontecilla Tomas Ros Eric Acquaviva Tobias Banaschewski Sarah Baumeister Elisa Bousquet Aurore Bussalb Marie Delhaye Richard Delorme Renate Drechsler Allison Goujon Alexander Häge Anna Kaiser Louis Mayaud Konstantin Mechler Caroline Menache Olivier Revol Friederike Tagwerker Susanne Walitza Anna Maria Werling Stephanie Bioulac Daniel Brandeis 《Journal of child psychology and psychiatry, and allied disciplines》2022,63(2):187-198
48.
49.
Pleun Hombrink Yotam Raz Michel G. D. Kester Renate de Boer Bianca Weißbrich Peter A. von dem Borne Dirk H. Busch Ton N. M. Schumacher J. H. Frederik Falkenburg Mirjam H. M. Heemskerk 《European journal of immunology》2013,43(11):3038-3050
The low frequency of antigen‐specific naïve T cells has challenged numerous laboratories to develop various techniques to study the naïve T‐cell repertoire. Here, we combine the generation of naïve repertoire‐derived antigen‐specific T‐cell lines based on MHC‐tetramer staining and magnetic‐bead enrichment with in‐depth functional assessment of the isolated T cells. Cytomegalovirus (CMV) specific T‐cell lines were generated from seronegative individuals. Generated T‐cell lines consisted of a variety of immunodominant CMV‐epitope‐specific oligoclonal T‐cell populations restricted to various HLA‐molecules (HLA‐A1, A2, B7, B8, and B40), and the functional and structural avidity of the CMV‐specific T cells was studied. Although all CMV‐specific T cells were isolated based on their reactivity toward a specific peptide‐MHC complex, we observed a large variation in the functional avidity of the MHC‐tetramer positive T‐cell populations, which correlated with the structural avidity measured by the recently developed Streptamer koff‐rate assay. Our data demonstrate that MHC‐tetramer staining is not always predictive for specific T‐cell reactivity, and challenge the sole use of MHC‐tetramers as an indication of the peripheral T‐cell repertoire, independent of the analysis of functional activity or structural avidity parameters. 相似文献
50.
Allergic multimorbidity of asthma,rhinitis and eczema over 20 years in the German birth cohort MAS
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![点击此处可从《Pediatric allergy and immunology》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Hannah Gough Linus Grabenhenrich Andreas Reich Nora Eckers Oliver Nitsche Dirk Schramm John Beschorner Ute Hoffmann Antje Schuster Carl‐Peter Bauer Johannes Forster Fred Zepp Young‐Ae Lee Renate L. Bergmann Karl E. Bergmann Ulrich Wahn Susanne Lau Thomas Keil MAS study group 《Pediatric allergy and immunology》2015,26(5):431-437