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31.
Yamazaki M Aoki H Iwade Y Matsumoto M Yamada K Yamamoto H Suzuki M Hiramatsu R Minagawa H 《Japanese journal of infectious diseases》2012,65(2):111-116
We developed an enrichment medium for use with the loop-mediated isothermal amplification (LAMP) assay (enrichment media + LAMP assay) to quickly increase a very small number of Vibrio parahaemolyticus cells to the detection limit of the assay. Thirty-nine different enrichment media were prepared based on evaluating 12 potential ingredients. From our assessment of the 39 media, enrichment medium #36, which contained 2% sodium chloride, 1% proteose peptone no. 2, 0.1% trehalose, 0.5% α-ketoglutaric acid, 0.25% pyruvic acid, and 0.5% yeast extract (pH 8.6), was found to be most effective at enhancing the proliferation of V. parahaemolyticus during incubation for 3 h at 40oC. We compared the detection limits of the LAMP assay, the enrichment medium #36 + LAMP assay, and the cultivation method using bacterial cell and spiked shrimp sample tests. The detection limits of the LAMP assay, the medium #36 + LAMP assay, and the cultivation method were 10(3), 10(0)-10(-1), and 10(-1) CFU ml(-1), respectively. Enrichment medium #36 promoted a 10(3)- to 10(4)-fold increase in the bacterial population, and the detection limit of the enrichment media + LAMP assay was the same as that of the cultivation method. 相似文献
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Shun-ichi Chitose Masahiro Haraguchi Shuji Nagata Reiji Katayama Kiminori Sato Mioko Fukahori Shintaro Sueyoshi Takashi Kurita Toshi Abe Tadashi Nakashima 《Dysphagia》2014,29(3):387-395
The purpose of this study was to analyze passive motion of the para- and retropharyngeal space (PRS) during swallowing using dynamic magnetic resonance imaging (MRI). We conducted a preliminary study involving 30 healthy volunteers who underwent dynamic MRI. Consecutive MRI axial images were obtained by examining the plane parallel to the hard palate at the level of the anterior inferior corner of C2. Anterior displacement of the posterior pharyngeal wall (PPW) was measured as a motion index of pharyngeal contraction. The displacement and internal angle of the bilateral external and internal carotid arteries (ECA and ICA) and the bilateral centroids of the PRS area, as well as the increase in PRS area, were calculated at rest and at maximum pharyngeal contraction. In most participants, the bilateral ECA, ICA, and centroids were anterointernally displaced by pharyngeal contraction. The normalized ECA displacement (r = 0.64, r 2 = 0.41), normalized ICA displacement (r = 0.60, r 2 = 0.37), and normalized centroid displacement (r = 0.43, r 2 = 0.19) were more than moderately positively correlated with the normalized PPW displacement. The normalized PRS area increase (r = 0.35, r 2 = 0.12) was weakly positively correlated with the normalized PPW displacement. These results revealed that PRS area increased as the ECA and ICA were drawn anterointernally via its passive motion by pharyngeal contraction. 相似文献
34.
Kumiko Shimizu Tomoharu Sano Reiji Kubota Norihiro Kobayashi Maiko Tahara Tomoko Obama Naoki Sugimoto Tetsuji Nishimura Yoshiaki Ikarashi 《Toxins》2014,6(1):168-179
Microcystins, which are cyclic heptapeptides produced by some cyanobacterial species from algal blooms, strongly inhibit serine/threonine protein phosphatase and are known as hepatotoxins. Microcystins have many structural variations, yet insufficient information is available on the differences in the cytotoxic potentials among the structural variants. In this study, the cytotoxicities of 16 microcystin variants at concentrations of 0.03–10 μg/mL to primary cultured rat hepatocytes were determined by measuring cellular ATP content, and subsequently determined by their 50% inhibitory concentration (IC50). Differences in the amino acid constituents were associated with differences in cytotoxic potential. [d-Asp3, Z-Dhb7] microcystin-LR exhibited the strongest cytotoxicity at IC50 of 0.053 μg/mL among the microcystin variants tested. Furthermore, [d-Asp3, Z-Dhb7] microcystin-HtyR was also highly cytotoxic. These results suggest that both d-Asp and Z-Dhb residues are important in determining the cytotoxic potential of microcystin variants. 相似文献
35.
Cytopathologic characteristics and differential diagnostic considerations of osteolytic myxopapillary ependymoma
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Toshitetsu Hayashi M.D. Ph.D. Reiji Haba M.D. Ph.D. Yoshio Kushida M.D. Ph.D. Kyuichi Kadota M.D. Ph.D. Naomi Katsuki M.D. Kenji Bando M.D. Ph.D. Shinsuke Shibuya M.D. Toru Matsunaga C.T. 《Diagnostic cytopathology》2014,42(9):778-783
Myxopapillary ependymoma (MPE) is a rare variant of conventional ependymoma found predominantly in the sacrococcygeal region in young adults and characterized by its distinct epithelial and stromal components (WHO grade I designation). MPE with extensive osteolysis is extremely uncommon and only up to 40 cases have been documented. A case is presented here in which imprint smears of a sacral tumor in an 18‐year‐old man revealed complex papillary structures, small loose clusters, or cord‐like structures of bland tumor cells embedded in a myxoid or mucinous background. The tumor cells possessed uniformly round nuclei with a smooth nuclear outline, fine granular chromatin, and small nucleoli. Slender cytoplasmic fibrillary processes and occasional intracytoplasmic vacuoles were observed. A cytologic diagnosis of a MPE was suggested and histochemical and immunohistochemical studies were conducted on formalin‐fixed, paraffin‐embedded material. Immunohistochemically, the tumor cells showed diffuse and strong membranous and cytoplasmic staining for cytokeratin AE1/AE3, glial fibrillary protein, and S‐100 protein, but negative for epithelial membrane antigen, pan‐neuroendocrine markers (i.e., NSE, chromogranin A, synaptophysin), or brachyury. The proliferative index with MIB‐1 was around 10%. The diagnosis of osteolytic MPE was confirmed based on cytopathologic, histopathological, immunohistochemical results, radiologic findings, and the location of the tumor. We demonstrated here the cytopathological features of osteolytic MPE with emphasis on differential diagnostic considerations. Diagn. Cytopathol. 2014;42:778–783. © 2013 Wiley Periodicals, Inc. 相似文献
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Tetsuo Mitsui Naoto Fujita Yuhki Koga Reiji Fukano Tomoo Osumi Asahito Hama Katsuyoshi Koh Harumi Kakuda Masami Inoue Takahiro Fukuda Hiromasa Yabe Junko Takita Akira Shimada Yoshiko Hashii Atsushi Sato Yoshiko Atsuta Yoshinobu Kanda Junji Suzumiya Ryoji Kobayashi 《Pediatric blood & cancer》2020,67(4)
38.
Tetsuo Sekine Kyoichiro Tsuchiya Hiroyuki Uchinuma Sayaka Horiuchi Megumi Kushima Sanae Otawa Hiroshi Yokomichi Kunio Miyake Yuka Akiyama Tadao Ooka Reiji Kojima Ryoji Shinohara Shuji Hirata Zentaro Yamagata The Japan Environment Childrens Study Group 《Journal of diabetes investigation.》2022,13(4):687
Aims/IntroductionGestational diabetes mellitus (GDM) is one of the most common complications of pregnancy and is associated with adverse pregnancy outcomes. This study aimed to explore the associations between glycated hemoglobin (HbA1c) levels at the early stage of pregnancy and the GDM risk among non‐diabetic women in a nationwide study in Japan. In addition, the relationship between GDM and adverse pregnancy outcomes was also analyzed.Materials and MethodsThis cohort study (n = 89,799) used data from the Japan Environment and Children’s Study. We stratified the participants into four groups according to HbA1c levels at an early stage of pregnancy. We investigated the association of HbA1c at an early stage of pregnancy with the risk of GDM, and of GDM with the risk of some representative adverse pregnancy outcomes, using the multiple logistic regression model with adjustment for potential confounders.ResultsThe adjusted odds ratio for GDM per 0.1 percentage point increase in HbA1c (%) was 1.20. The adjusted odds ratio for developing GDM was significantly increased in women from the HbA1c 5.0–5.4% category. GDM significantly increased the adjusted odds ratio for adverse pregnancy outcomes, such as hypertensive disorders of pregnancy, polyhydramnios and premature birth.ConclusionsHigh‐normal HbA1c levels at the early stage of pregnancy are significantly associated with GDM risk in women in Japan. GDM was significantly associated with adverse pregnancy outcomes. 相似文献
39.
Naoya Nakano Maki Torimoto Hiroshi Sampei Reiji Yamashita Ryota Yamano Koki Saegusa Ayaka Motomura Kaho Nagakawa Hideaki Tsuneki Shuhei Ogo Yasushi Sekine 《RSC advances》2022,12(15):9036
With increasing expectations for carbon neutrality, dry reforming is anticipated for direct conversion of methane and carbon dioxide: the main components of biogas. We have found that dry reforming of methane in an electric field using a Pt/CeO2 catalyst proceeds with sufficient rapidity even at a low temperature of about 473 K. The effect of the electric field (EF) on dry reforming was investigated using kinetic analysis, in situ DRIFTs, XPS, and DFT calculation. In situ DRIFTs and XPS measurements indicated that the amount of carbonate, which is an adsorbed species of CO2, increased with the application of EF. XPS measurements also confirmed the reduction of CeO2 by the reaction of surface oxygen and CH4. The reaction between CH4 molecules and surface oxygen was promoted at the interface between Pt and CeO2.In the dry reforming of methane in an electric field, the reaction between CH4 molecules and surface oxygen was promoted at the interface between Pt and CeO2. 相似文献
40.
Susumu Hijioka Kazuo Hara Nobumasa Mizuno Hiroshi Imaoka Takeshi Ogura Shin Haba Mohamed A. Mekky Vikram Bhatia Waki Hosoda Yasushi Yatabe Yasuhiro Shimizu Yasumasa Niwa Masahiro Tajika Shinya Kondo Tsutomu Tanaka Kiichi Tamada Kenji Yamao 《Journal of hepato-biliary-pancreatic sciences》2012,19(6):650-655