傣药小灯台中的吲哚生物碱

从傣药小灯台(Winchia catophylla A. DC.)中分到4个吲哚生物碱,经理化常数测定,光谱分析和化学转化,分别鉴定为echitamine chloride(Ⅰ),echitamidine(Ⅱ),N_B-demethyl-echitamine(Ⅲ)和22-O-acetyl-N_b--demethyl-echitamine(Ⅳ),其中Ⅳ为新的吲哚生物碱。     

HPLC定量分析制剂中雷公藤氯内酯醇含量

雷公藤氯内酯醇(T_4)是从雷公藤中分离得到的一个新化合物,但含量很低,因此进行了以雷公藤内酯醇(To)为原料合成T_4的研究。T_4具有较强的免疫抑制活性,且毒性低于雷公藤内酯醇,有希望开发成新药在临床应用。为了控制T_4制剂的质量,本文系统地研究了反相高效液相色谱法分离和测定T_4的条件,结果以甲醇-水(1:1)作流动相,能满意地分离和测定T_4的含量,最小检测量为10 ng,采用内标法定量,方法快速、准确。     

烟酰胺类和吡啶丙烯酰胺类衍生物的合成及扩血管活性

烟酰胺类和吡啶丙烯酰胺类衍生物的合成及扩血管活性傅焕建，朱莉亚，林紫云（中国医学科学院，中国协和医科大学药物研究所，北京１０００５０）高血压流行病学的调查表明［１］，我国每年至少有９５万以上高血压病人，所以高血压病是一类常见病，多发病。目前临床应用的...     

大戟根化学成分的研究

从中药大戟(Euphorbia pekinensis)根中分得9个化合物,经理化常数和波谱数据分析,分别鉴定为羊毛甾醇(I),3-甲氧基-4-羟基反式苯丙烯酸正十八醇酯(II),β-谷甾醇(III),伞形花内酯(IV),2,2′-二甲氧基-3,3′二羟基-5,5′-氧-6,6′-联苯二甲酸酐(V),d-松脂素(VI),槲皮素(VII),3,4-二甲氧基苯甲酸(VII)和3,4-二羟基苯甲酸(IX)。其中II和V为两个未见文献报道的新化合物,其余化合物均为首次从该植物中分离得到,VI为首次从该属植物中分得的木脂素类化合物。     

白花前胡中白花前胡甙和Pd-C-I的分离和鉴定

从白花前胡(Peucedanum,praeruptorum)根中分得7个化合物,经化学方法和光谱分析分别鉴定为Pd-C-I(I),白花前胡甙(II),香草酸(III),没食子酸(IV),nodakenin(V),rutarin(VI)和isorutarin(VII)。II为新化合物,其化学结构为4-O-β-D-吡喃葡萄糖基-3-甲氧基苯丙酮,命名为白花前胡甙。I为首次从白花前胡中分得的线型二氢吡喃香豆素类化合物,这对前胡属植物化学分类学有一定意义。还利用2DNMR纠正了文献中关于化合物I和VII的个别碳信号归属的错误。     

Analysis of extrachromosomal DNA from normal and tumor cells

Two independent methods (pulse-electrophoresis of intact DNA isolated from agarose-encapsulated cells and differential extraction of intact DNA from Celite-immobilized nucleoproteins) yielded evidence of the presence of free (extrachromosomal) DNA in cells of various origin, both malignant and normal, cultured and isolated from animal tissues. Free DNA from all cell types studied form three discrete electrophoretic bands of 400, 250–300, and 50 thousand base pairs. According to the data on³H-thymidine incorporation, free and chromosomal DNA differ in their metabolic properties. By means of the polymerase chain reaction using c-myc and L-myc primers it is established that free DNAs of HT1080 cells contain structural genes. Application of the method of conformational polymorphism of single-strand fragments revealed their identity to the corresponding genes localized in the chromosomes. Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 119, No 5, pp. 533–536, May, 1995 Presented by I. P. Ashmarin, Member of the Russian Academy of Medical Sciences     

经颅打孔引流治疗高血压脑出血105例

1 临床资料起病24 h内住院高血压脑出血患者209例. 头颅CT或MRI扫描示幕上出血,微创打孔组105(男59,女46)例,年龄41～79(55±6.9)岁,脑叶19例,基底节区59例,丘脑21例,脑室6例,伴破入脑室25例,出血量30～50 mL 28例,51～70 mL 54例,≥71 mL 23例;锥颅打孔组104(男49,女55)例,年龄43～77(53±7.6)岁, 脑叶14例,基底节区63例,丘脑27例,伴破入脑室27例,出血量30～50 mL 38例;51～70 mL 49例;≥71 mL 37例,两组临床相似, 均给常规基础及药物治疗.     

微波和磁疗治疗膝关节骨性关节炎300例

1临床资料①对象:本组骨性关节炎患者300(男130,女170)例,年龄36～78(56±1.0)岁,病程1 mo～10 a,其中左膝120例,右膝112例,双膝68例.临床表现为关节疼痛、僵直、活动受限,偶尔会关节肿胀、关节腔积液.X线表现为关节间隙变窄,关节软骨下骨质致密,骨密度增高,骨小梁有断裂,髁间嵴变尖及髌骨后缘和外侧缘增生形成骨刺[1].     

Effect of thymoptin on hemostasis and fibrinolysis in rats with experimental nephritis

Immune nephritis in rats was induced by administration of nephrotoxic rabbit antiserum. The development of severe renal inflammation (proteinuria, edema, lipemia, increased erythrocyte sedimentation rate, and 30% mortality) was accompanied by hypercoagulation and inhibition of fibrinolysis. Repeated subcutaneous injections of thymoptin in a low dose of 0.1 μg/200 g (5 injections every other day) increased the severity of inflammation and prethrombotic state of the blood. Lengthening the period between injections (5 injections at 5-day intervals) was followed by a tendency toward attenuation of nephritis and correction of hypercoagulation. In healthy rats, thymoptin produced an opposite effect on hemostasis, which was manifested in moderate stimulation of fibrinolysis and hypocoagulation. Translated from Byulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 146, No. 7, pp. 8–12, July, 2008