端粒酶与女性生殖功能

端粒酶是细胞中负责端粒延长的一种酶,通过合成染色体末端的DNA赋予细胞复制的永生性。其在保持端粒稳定、基因组完整、细胞长期的活性和潜在的继续增殖能力等方面有重要作用,端粒/端粒酶复合物的结构和功能的改变将会导致不同的疾病,尤其是癌症的发生。在人类正常生殖细胞、早期胚胎、干细胞、高度增殖体细胞和许多癌细胞中均有不同程度的端粒酶活性表达,并受到严密的调控。在女性生殖功能调控下,端粒酶活性表达在卵泡的发育及闭锁、子宫内膜的周期性变化、卵巢储备功能及性激素调节等方面发挥着重要的作用,并可在一定程度上预测卵巢储备功能及体外受精妊娠结局。     

Inhibitory effect of emodin on human hepatoma cell line SMMC-7721 and its mechanism

BackgroundDa Huang (Radix et Rhizoma Rhei) is the dried root or rhizome of Rheum palmatum L., Rheum tanguticum Maxim ex Balf. or Rheum officinale Braill of family Polygonaceae. It has heat clearing, damp drying, fire purging and toxin removing effects. Because of its definite curative efficacy, it has been widely applied in clinical settings.ObjectiveTo study the inhibitory effect of emodin on human hepatoma cell line SMMC-7721 and its mechanism.MethodsMTT assay, flow cytometry and electron microscopy were used to investigate the inhibitory effect of different concentrations of emodin on human hepatoma cell line SMMC-7721.Results12 h, 24 h and 48 h after the action of 20, 40 and 80 umol/L emodin on SMMC-7721 cells, the proliferation of human hepatoma SMMC-7721 cells was inhibited; the inhibitory effects showed time-and concentration-dependence. 48 h after the action of different concentrations of emodin on SMMC-7721 cells, cells in G2/M phase increased significantly, while the proportion of S phase cells gradually declined.ConclusionEmodin can inhibit human hepatoma cell line SMMC-7721.     

IL-33 signaling contributes to the pathogenesis of myeloproliferative neoplasms

Myeloproliferative neoplasms (MPNs) are characterized by the clonal expansion of one or more myeloid cell lineage. In most cases, proliferation of the malignant clone is ascribed to defined genetic alterations. MPNs are also associated with aberrant expression and activity of multiple cytokines; however, the mechanisms by which these cytokines contribute to disease pathogenesis are poorly understood. Here, we reveal a non-redundant role for steady-state IL-33 in supporting dysregulated myelopoiesis in a murine model of MPN. Genetic ablation of the IL-33 signaling pathway was sufficient and necessary to restore normal hematopoiesis and abrogate MPN-like disease in animals lacking the inositol phosphatase SHIP. Stromal cell–derived IL-33 stimulated the secretion of cytokines and growth factors by myeloid and non-hematopoietic cells of the BM, resulting in myeloproliferation in SHIP-deficient animals. Additionally, in the transgenic JAK2^V617F model, the onset of MPN was delayed in animals lacking IL-33 in radio-resistant cells. In human BM, we detected increased numbers of IL-33–expressing cells, specifically in biopsies from MPN patients. Exogenous IL-33 promoted cytokine production and colony formation by primary CD34⁺ MPN stem/progenitor cells from patients. Moreover, IL-33 improved the survival of JAK2^V617F-positive cell lines. Together, these data indicate a central role for IL-33 signaling in the pathogenesis of MPNs.     

Suppressor of cytokine signaling 3 A+930-->G (rs4969168) polymorphism is associated with apolipoprotein A1 and low-density lipoprotein cholesterol

This study aimed to detect the association of the suppressor of cytokine signaling 3 gene (SOCS3) A+930-->G (rs4969168) single nucleotide polymorphism (SNP) and environmental factors with serum lipid levels in the Han and Mulao populations. Genotyping of the SOCS3 A+930-->G (rs4969168) SNP was performed in 752 of Han and 690 of Mulao participants using polymerase chain reaction and restriction fragment length polymorphism. The genotype and allele frequencies were significantly different between the Han and Mulao populations (GG, 57.71% vs. 51.16%, GA, 36.97% vs. 41.16%, AA, 5.32% vs. 7.68%, P = 0.023; G, 76.20% vs. 71.74%, A, 23.80% vs. 28.26%; P = 0.006; respectively). Serum apolipoprotein (Apo) A1 levels in Han were different among the genotypes (P < 0.05). Subgroup analyses showed that the levels of ApoA1 in Han females, and ApoA1 and low-density lipoprotein cholesterol (LDL-C) in Mulao males were different among the genotypes (P < 0.05). Serum lipid parameters were also associated with several environmental factors in both ethnic groups (P < 0.05-0.001). These findings suggest that there may be a racial/ethnic- and/or sex-specific association between the SOCS3 A+930-->G (rs4969168) SNP and serum lipid parameters in some populations.     

QZS－Ⅲ自动视野计及其临床初步应用

我院自１９９３年７月至１９９５年５月应用ＱＺＳ－Ⅲ自动视野计检查４２６人（８５２眼），主要对象为青光眼及可疑青光眼２８８例，其次为各类眼底病、颅内占位性病变、视神经疾病等１３８例。视野检查结果，青光眼及可疑青光眼２８８例中有鼻侧阶梯暗点、旁中心及楔形暗点、弓形、环形暗点、管状视野等青光眼中时期视野改变、非青光眼视野改变（生理盲点扩大）等。各类眼底疾病及颅内病变１３８例，其视野改变与视网膜病变部位，斑点、严重程度等密切相关。     

速效止血粉治疗急性创伤出血的临床研究

目的 观察速效止血粉治疗急性创伤出血的临床疗效和安全性。方法 把速效止血粉直接洒在出血部位，观察止血效果、止血时间和起效时间。结果 速效止血粉适用于各种创伤出血，尤其在中、大动脉出血的止血效果方面具有常规方法不能比拟的优势。结论 速效止血粉是治疗各种急性出血的一种安全、有效的药物。     

应激性高血糖对急性脑梗死短期临床预后的影响

目的　探讨应激性高血糖 (SHG)对急性脑梗死 (ACI)患者短期临床预后的影响。方法　对 68例伴 SHG(SHG组 )和 66例血糖正常 (NBG)的 ACI患者 (NBG组 )进行研究 ,比较两组短期预后和临床事件发生率。结果　 SHG组预后差、住院期间临床事件发生率高于 NBG组 ,组间差异显著。结论　 SHG对 ACI患者预后有明显影响 ,并使临床事件发生率增高 ,可作为短期临床预后不良的预测指标     

三七皂甙在严重烧伤大鼠延迟复苏过程中调控效应

目的:探讨三七皂甙在烧伤休克延迟复苏大鼠中的抗渗作用及对肿瘤坏死因子α、白细胞介素1β、白细胞介素6的调控。方法:实验于2003-12/2005-02在第二军医大学长海医院烧伤中心实验室完成。选用36只健康成年的SD大鼠,采用随机数字法分为假烫组、烫伤组及治疗组,每组12只。①制备烧伤模型:用恒温水烫仪以100℃水烫背部13s,造成大鼠30%TBSAⅢ度烧伤(病理证实)。②制备烧伤休克延迟复苏模型:烫伤大鼠均于伤后6h腹腔注射乳酸林格液进行延迟复苏,总量按Parkland公式4mL/(kg·%)计算。③治疗组伤后0.5h腹腔注射三七皂甙(100mg/kg);假烫组大鼠以38℃水模拟烫伤过程。④伤后24h采用干湿重法检测肺脏、肝脏及肠的含水量,组织含水量(%)=(湿重-干重)/湿重×100%。测定各上清液相应的伊文思蓝浓度,以此来表示各脏器血管通透性的大小,伊文思蓝含量(μg/g干重组织)=伊文思蓝浓度(mg/L)×10/[1-组织含水量(%)]。采用酶联免疫吸附法(ELISA方法)分别测定血浆中肿瘤坏死因子α、白细胞介素1β及白细胞介素6的水平。结果:36只大鼠均进入结果分析。①伤后24h治疗组大鼠的肺脏、肝脏及小肠的含水量明显低于烫伤组相应脏器含水量((79.91±1.76)%比(82.04±2.05)%,(71.72±1.75)%比(73.63±1.61)%,(76.52±1.43)%比(80.31±3.69)%,P<0.05)。②治疗组大鼠的肺脏、肝脏及小肠的血管通透性(伊文思蓝μg/g干重组织)明显低于烫伤组大鼠相应脏器血管通透性(313.05±19.30比457.86±43.33,156.72±17.75比243.63±26.74,236.52±26.43比380.31±33.69,P<0.01)。③治疗组大鼠血浆中肿瘤坏死因子α、白细胞介素1β及白细胞介素6的水平均显著低于烫伤组血浆中各细胞因子水平[(258.91±20.4)ng/L比(612.30±75.03)ng/LQ(101.37±29.86)ng/L比(208.45±49.04)ng/LQ(433.65±31.78)ng/L比(726.46±38.61)ng/L,P<0.01]。结论:大鼠严重烧伤后,早期使用三七皂甙,能下调肿瘤坏死因子α、白细胞介素1β及白细胞介素6在血浆中的总体水平,控制烧伤后过度全身炎症反应,有助于减轻脏器的水肿,从而减轻烧伤早期脏器损害。