全文获取类型
收费全文 | 468篇 |
免费 | 18篇 |
国内免费 | 9篇 |
专业分类
儿科学 | 9篇 |
妇产科学 | 5篇 |
基础医学 | 63篇 |
口腔科学 | 13篇 |
临床医学 | 41篇 |
内科学 | 92篇 |
皮肤病学 | 22篇 |
神经病学 | 4篇 |
特种医学 | 154篇 |
外科学 | 31篇 |
综合类 | 2篇 |
预防医学 | 23篇 |
眼科学 | 2篇 |
药学 | 18篇 |
肿瘤学 | 16篇 |
出版年
2022年 | 4篇 |
2021年 | 6篇 |
2020年 | 9篇 |
2019年 | 3篇 |
2018年 | 15篇 |
2017年 | 3篇 |
2016年 | 10篇 |
2015年 | 9篇 |
2014年 | 12篇 |
2013年 | 18篇 |
2012年 | 7篇 |
2011年 | 15篇 |
2010年 | 13篇 |
2009年 | 13篇 |
2008年 | 14篇 |
2007年 | 15篇 |
2006年 | 17篇 |
2005年 | 7篇 |
2004年 | 2篇 |
2003年 | 4篇 |
2002年 | 6篇 |
2001年 | 4篇 |
2000年 | 4篇 |
1999年 | 4篇 |
1998年 | 16篇 |
1997年 | 23篇 |
1996年 | 22篇 |
1995年 | 14篇 |
1994年 | 21篇 |
1993年 | 12篇 |
1992年 | 4篇 |
1991年 | 9篇 |
1990年 | 6篇 |
1989年 | 22篇 |
1988年 | 18篇 |
1987年 | 10篇 |
1986年 | 19篇 |
1985年 | 12篇 |
1984年 | 13篇 |
1983年 | 4篇 |
1982年 | 12篇 |
1981年 | 6篇 |
1980年 | 6篇 |
1979年 | 2篇 |
1978年 | 5篇 |
1977年 | 9篇 |
1976年 | 6篇 |
1975年 | 6篇 |
1961年 | 1篇 |
1935年 | 1篇 |
排序方式: 共有495条查询结果,搜索用时 31 毫秒
101.
102.
Quantification of blood flow with dynamic MR imaging and presaturation bolus tracking 总被引:1,自引:0,他引:1
A technique is described for rapid imaging of blood flow and dynamic measurement of its velocity. The method is a combination of bolus tracking and low-flip-angle gradient-echo cine angiography. This method provides precise determination of velocity with high temporal resolution in a single measurement. Unlike what occurs in phase imaging techniques, flow is displayed directly, eliminating potential errors that result from non-flow-related sources of phase shifts. Manipulation of raw data sets is avoided. Results obtained from a flow phantom, healthy volunteers, and a patient with an aortic aneurysm demonstrate the capability of the technique to track flow at low and high velocities and to differentiate flowing blood from thrombus. Because of its conceptual simplicity, rapidity, and lack of susceptibility to extraneous phase shifts, this technique may prove ideal for in vivo flow measurement and evaluation of flow patterns. 相似文献
103.
104.
Jo?o Carlos Regazzi Avelleira Fred Bernardes Filho Maria Victória Quaresma Francisco Reis Vianna 《Anais brasileiros de dermatologia》2014,89(3):515-518
The record of the first cases of leprosy in Rio de Janeiro dates from the
seventeenth century. The first local host of leprosy patients was created from
1741, and the first colonies hospitals were built in the early twentieth
century, in order to avoid contagion of the population. The first structures
dedicated to research also date from this time: the Leprosy International
Institute, the Leprology Institute, and the Leprosy Laboratory of the Oswaldo
Cruz Foundation, where the most prestigious leprologists of Rio de Janeiro
worked. Currently, investigations are focused on the Oswaldo Cruz Foundation;
additionally, leprosy patients are treated at municipal health centers and state
hospitals, and former colony hospitals only accept patients with severe
disabilities. 相似文献
105.
This is an exploratory study based on a qualitative approach. The objective was to investigate why HIV positive pregnant women do not follow the treatment which is recommended by the Health Ministry during the prenatal period. The individuals participating of this investigation were seven HIV positive women who, during their pregnancy, did not follow the prophylaxis treatment currently recommended. The data were collected by means of semi-structured interviews. Categories based on what these individuals reported were then stipulated and the results showed that women are in a vulnerable position regarding the negotiation of safe sex with their partners. Moreover, low social and economical conditions and lack of affection lead women to become victims of HIV/AIDS. 相似文献
106.
A comparison between activated protein C and des-1-41-light chain- activated protein C in reactions with type 1 plasminogen activator inhibitor 总被引:2,自引:0,他引:2
This study investigates the role of the gamma-carboxyglutamic acid (gla) containing domain of activated protein C in interactions with both platelet-derived and purified type 1 plasminogen activator inhibitor (PAI-1). The activity of human platelet PAI-1 was neutralized to the same extent by bovine activated protein C and bovine des-1-41- light chain-activated protein C. Both forms of activated protein C formed SDS-stable, divalent-cation independent complexes with platelet PAI-1, as demonstrated by immunoblotting using antibodies directed to either protein C or PAI-1. Since activated protein C neutralized PAI-1, the potential inhibition of the enzyme by PAI-1 was studied. Purified PAI-1 inhibited the amidolytic activity of bovine-activated protein C and bovine des-1-41-light chain-activated protein C with a k2 of 2.85 X 10(4) M-1 sec-1 for both proteins. These data suggest that the gla domain of activated protein C is not required for neutralization of PAI- 1 activity, for complex formation with PAI-1, or for inhibition of the amidolytic activity of activated protein C by PAI-1. 相似文献
107.
Schumann RR; Pfeil D; Lamping N; Kirschning C; Scherzinger G; Schlag P; Karawajew L; Herrmann F 《Blood》1996,87(7):2805-2814
108.
The treatment of malignant histiocytosis 总被引:1,自引:0,他引:1
Tseng A Jr; Coleman CN; Cox RS; Colby TV; Turner RR; Horning SJ; Rosenberg SA 《Blood》1984,64(1):48-53
Twenty-four consecutive cases of malignant histiocytosis (MH) treated at Stanford Medical Center between 1973 and 1983 have been reviewed. Most patients presented with systemic symptoms (91%) and advanced disease (stage IV, 80%). Multiple organ involvement was common. In six cases, pathologic tissue was further characterized by frozen section immune histochemistry, using a panel of monoclonal antibodies known to react with monocytes and macrophages, as well as a variety of hematopoietic cells. One case expressed a mature monocyte/macrophage phenotype; three cases were considered null cell or primitive lesions; and two cases were identified as probable T cell lymphomas. Seven patients underwent splenectomy. Two patients died prior to any treatment. Twenty-two patients were treated with CHOP (cyclophosphamide, Adriamycin, vincristine, prednisone) +/- bleomycin (B), +/- midcycle high-dose methotrexate (HD-MTX) with leucovorin rescue. Seven patients received prophylactic intrathecal MTX. Of 22 evaluable patients, there was a 68% complete response rate (CR), a 23% partial response rate (PR), and a 9% no response rate (NR). Median duration of CR was 30+ months; median duration of PR was 2.4 months. Median survival for patients attaining a CR has not been reached v 3 months for the PR and NR groups. For all 24 patients, median survival was 2 years, with a 5-year actuarial survival of 40%. Multivariate analysis revealed that a platelet count less than 150,000 (P Cox = .005) and the dose of drug delivered (P Cox = .057) were the most important prognostic factors. Prophylactic intrathecal MTX therapy and splenectomy did not influence survival. Although MH is an aggressive disease with a poor prognosis, it is potentially curable. Systematic and aggressive treatment should further improve the outcome. 相似文献
109.
Mechanisms of human cytomegalovirus infection with a focus on epidermal growth factor receptor interactions 下载免费PDF全文
Aline Semblano Carreira Falcão Pedro Fernando da Costa Vasconcelos Dorotéa de Fátima Lobato da Silva João de Jesus Viana Pinheiro Luiz Fábio Magno Falcão Juarez Antonio Simões Quaresma 《Reviews in medical virology》2017,27(6)
Human cytomegalovirus (HCMV) is a widespread opportunistic herpesvirus that causes severe diseases in immunocompromised individuals. It has a high prevalence worldwide that is linked with socioeconomic factors. Similar to other herpesviruses, HCMV has the ability to establish lifelong persistence and latent infection following primary exposure. HCMV infects a broad range of cell types. This broad tropism suggests that it may use multiple receptors for host cell entry. The identification of receptors used by HCMV is essential for understanding viral pathogenesis, because these receptors mediate the early events necessary for infection. Many cell surface components have been identified as virus receptors, such as epidermal growth factor receptor (EGFR), which is characterized by tyrosine kinase activity and plays a crucial role in the control of key cellular transduction pathways. EGFR is essential for HCMV binding, signaling, and host cell entry. This review focuses on HCMV infection via EGFR on different cell types and its implications for the cellular environment, viral persistence, and infection. 相似文献
110.
Paediatric nodal marginal zone B‐cell lymphadenopathy of the neck: a Haemophilus influenzae‐driven immune disorder? 下载免费PDF全文
Philip M Kluin Anton W Langerak Jannetta Beverdam‐Vincent Willemina RR Geurts‐Giele Lydia Visser Bea Rutgers Ed Schuuring Joop Van Baarlen King H Lam Kees Seldenrijk Robby E Kibbelaar Peter de Wit Arjan Diepstra Stefano Rosati Max M van Noesel C Michel Zwaan Jarmo CB Hunting Mels Hoogendoorn Ellen J van der Gaag Joost W J van Esser Eveline de Bont Hanneke C Kluin‐Nelemans Rik H Winter Jerome R Lo ten Foe Adri GM van der Zanden 《The Journal of pathology》2015,236(3):302-314
Many hyperplasias and lymphomas of marginal zone B‐cells are associated with infection. We identified six children and one adolescent with cervical lymphadenopathy showing prominent polyclonal nodal marginal zone hyperplasia (pNMZH) and four adolescents with monoclonal paediatric nodal marginal zone lymphoma (pNMZL). The clonality status was assessed using BIOMED‐2‐IG PCR analysis. Haemophilus influenzae was identified in all six cases of pNMZH that could be tested by direct culture (N = 3) or a very sensitive PCR for the H. influenzae gyrase gene in frozen materials (N = 5). H. influenzae was not detected in three pNMZLs and 28 non‐specific reactive cervical lymph nodes of age‐matched controls, except for a single control node that was obtained during oropharyngeal surgery for a cleft palate showing very low copy numbers of H. influenzae. pNMZH patients were younger than pNMZL patients (median age 12 versus 21 years). pNMZH showed a prominent nodular appearance with variable fibrosis without acute inflammation. Within the nodules, the expanded germinal centres and variably sized marginal zones were colonized by activated B‐cells with weak expression of IgD and lack of CD10 and/or BCL6 expression. Some areas showed skewed light chain expression in plasma cells (4/5 cases lambda). In four cases tested, this was confirmed by flow cytometry for surface Ig (3/4 cases lambda). In contrast, pNMZL showed more extensive expansion of marginal zones by centrocytoid cells and often expression of BCL2 protein. Several H. influenzae strains are known to interact with the constant part of IgD on human B‐cells, leading to their polyclonal proliferation and activation. We speculate that in vivo stimulation of IgD+ marginal zone B‐cells by this bacterium may be implicated in this particular lymphadenopathy that should be distinguished from monoclonal pNMZL. Copyright © 2015 Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd. 相似文献