全文获取类型
收费全文 | 117557篇 |
免费 | 10500篇 |
国内免费 | 7443篇 |
专业分类
耳鼻咽喉 | 998篇 |
儿科学 | 1472篇 |
妇产科学 | 1559篇 |
基础医学 | 15290篇 |
口腔科学 | 2543篇 |
临床医学 | 14614篇 |
内科学 | 18727篇 |
皮肤病学 | 1388篇 |
神经病学 | 7367篇 |
特种医学 | 4312篇 |
外国民族医学 | 63篇 |
外科学 | 12550篇 |
综合类 | 17173篇 |
现状与发展 | 30篇 |
一般理论 | 11篇 |
预防医学 | 7037篇 |
眼科学 | 3195篇 |
药学 | 11815篇 |
68篇 | |
中国医学 | 5158篇 |
肿瘤学 | 10130篇 |
出版年
2024年 | 321篇 |
2023年 | 1736篇 |
2022年 | 4063篇 |
2021年 | 5074篇 |
2020年 | 3727篇 |
2019年 | 3410篇 |
2018年 | 3735篇 |
2017年 | 3359篇 |
2016年 | 3093篇 |
2015年 | 4689篇 |
2014年 | 5639篇 |
2013年 | 5137篇 |
2012年 | 7597篇 |
2011年 | 8404篇 |
2010年 | 5090篇 |
2009年 | 3949篇 |
2008年 | 5327篇 |
2007年 | 5745篇 |
2006年 | 5559篇 |
2005年 | 5515篇 |
2004年 | 3663篇 |
2003年 | 3346篇 |
2002年 | 2902篇 |
2001年 | 4079篇 |
2000年 | 4231篇 |
1999年 | 4231篇 |
1998年 | 2297篇 |
1997年 | 2019篇 |
1996年 | 1519篇 |
1995年 | 1325篇 |
1994年 | 1124篇 |
1993年 | 821篇 |
1992年 | 1678篇 |
1991年 | 1456篇 |
1990年 | 1322篇 |
1989年 | 1316篇 |
1988年 | 1162篇 |
1987年 | 1048篇 |
1986年 | 908篇 |
1985年 | 781篇 |
1984年 | 461篇 |
1983年 | 378篇 |
1982年 | 217篇 |
1981年 | 177篇 |
1980年 | 142篇 |
1979年 | 227篇 |
1973年 | 93篇 |
1972年 | 92篇 |
1971年 | 94篇 |
1968年 | 83篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
111.
112.
A Botulinum neurotoxin serotype A (BoNT/A) ELISA detection system was developed based upon an 11-mer cyclic peptide, termed C11-019, that was identified through peptide phage display technology. The assay employs a sandwich format using the C11-019 cyclic peptide attached to a PEMA (poly(ethylene maleic anhydride)) matrix as the capture phase and anti-BoNT/A polyclonal antibodies as the detection phase. Results reported demonstrate that the C11-019 peptide–polymer can specifically bind to BoNT/A with no cross-reactivity to other serotypes examined in assay buffers and a variety of body fluids and foodstuffs. When a highly sensitive chemiluminescent substrate was engaged, the detection of 1 pg/mL could be readily achieved within 3 h with a linear range of 0.1–1 ng/mL. These results demonstrate that an inexpensive peptide–polymer-based capture ELISA system can be used for rapid, sensitive and highly specific BoNT detection. 相似文献
113.
114.
ALBERTO Q FARIAS LUCIANA L GONÇALVES EDUARDO LR CANÇADO ANTONIO C SEGURO SILVIA B CAMPOS CLARICE P ABRANTES-LEMOS FLAIR J CARRILHO 《Journal of gastroenterology and hepatology》2006,20(1):147-152
Background and Aims: Primary biliary cirrhosis (PBC) might be complicated by osteoporosis, whose etiology remains unknown but seems to be multifactorial. Prevalence rates of 30% to 60% for distal renal tubular acidosis (DRTA) have been reported in PBC patients, generally as incomplete DRTA. Although it is undisputed that a reduced bone mineral density (BMD) is the expected outcome among patients who have been suffering from longstanding chronic metabolic acidosis, it is unclear if incomplete DRTA is also associated with metabolic bone disease in PBC patients. The present study was undertaken to compare the BMD of PBC patients with and without DRTA.
Methods: The BMD of 23 PBC patients (11 with DRTA and 12 without), all with normal clearance of creatinine, was assessed by dual energy radiograph absorptiometry. The diagnosis of DRTA was made if the urine pH was above 5.4 in all samples after the oral acid overload, showing tubular inability to acidify urine in the presence of test-induced systemic metabolic acidosis.
Results: Densitometric signs of osteoporosis were found in 82% of DRTA cases and in 83% of patients without DRTA (difference not significant). There were no significant differences in BMD measurement, T and Z scores of patients with and without DRTA.
Conclusions: The present study could not support a correlation between the presence of DRTA and the bone loss observed in PBC patients. 相似文献
Methods: The BMD of 23 PBC patients (11 with DRTA and 12 without), all with normal clearance of creatinine, was assessed by dual energy radiograph absorptiometry. The diagnosis of DRTA was made if the urine pH was above 5.4 in all samples after the oral acid overload, showing tubular inability to acidify urine in the presence of test-induced systemic metabolic acidosis.
Results: Densitometric signs of osteoporosis were found in 82% of DRTA cases and in 83% of patients without DRTA (difference not significant). There were no significant differences in BMD measurement, T and Z scores of patients with and without DRTA.
Conclusions: The present study could not support a correlation between the presence of DRTA and the bone loss observed in PBC patients. 相似文献
115.
目的探讨高频超声引导定位穿刺前臂浅静脉的应用价值。方法对40例常规浅静脉穿刺失败,采用高频探头在同一体表区域寻找并标记浅静脉走行及深度,确认进针方向后再次静脉穿刺,必要时采用实时引导。结果11例经高频超声标记、29例经高频超声实时引导静脉穿刺均一次成功。结论高频超声引导下静脉穿刺的成功率高,无并发症,是一种简便有效的方法,值得在临床推广应用。 相似文献
116.
Yanke Yu Wen Zhu Huiling Diao Chunxian Zhou Fanqing F Chen Jun Yang 《Toxicology in vitro》2006,20(6):959-965
Comet assay is a useful technique in the detection of DNA damages, particularly DNA strand breaks; and it has been utilized to show that a potent carcinogen N-methyl-N'-nitro-N-nitrosoguanidine (MNNG), can induce such damages. Recently, gammaH2AX foci formation has been suggested as another sensitive way to detect DNA double strand breaks (DSBs). However, there is no systematic comparison being conducted to evaluate the consistency of these two methods. Using MNNG as a model chemical, the sensitivity of neutral comet assay and gammaH2AX foci formation in detecting MNNG-induced damage was studied. It was found that at concentrations of 0.1 and 1 microg/ml, both methods can detect MNNG-induced damage in human amnion FL cells. However, at 0.1 microg/ml, comet assay revealed more percentage of cells with DNA damage than gammaH2AX fluorescence revealed. On the other hand, while gammaH2AX foci were readily formed at very early times by 10 microg/ml MNNG treatment, neutral comet assay did not detect any significant DNA damage at the same time points. In addition, 10 microg/ml MNNG induced a distinct whole nuclei staining pattern of gammaH2AX, a type of DNA damage which was not detected by neutral comet assay but could be detected by alkaline comet assay. Therefore, gammaH2AX may be used as a sensitive indicator for DNA damage. 相似文献
117.
肝移植术后胆道并发症的介入治疗 总被引:4,自引:1,他引:3
目的 探讨原位肝移植术后胆道并发症的介入治疗疗效。方法 回顾性分析我院2002年6月至2005年9月诊治的173例原位肝移植患者的临床资料。结果 术后出现胆道并发症14例(8.1%),其中胆管狭窄6例.胆管狭窄合并胆漏1例,胆泥淤积或结石3例,肝断面胆漏2例(劈离式肝移植患者),T管拔除后胆漏1例,Oddi括约肌功能失常1例。除1例胆道狭窄再次行肝移植,因发生严重感染导致肝功能衰竭死亡外.其余患者经介入治疗均获得满意的效果。结论 介入治疗是诊断和治疗肝移植术后胆道并发症的首选方法。 相似文献
118.
肝移植围手术期出凝血功能障碍的防治 总被引:3,自引:0,他引:3
目的 探讨肝移植围手术期出凝血功能障碍的防治。方法 回顾性分析我院 2 0 0 2年 6月~ 2 0 0 3年 12月施行的 6 1例肝移植病例。结果 6 1例肝移植术前肝功能ChildC级 35例 (5 7 4 % ) ,ChildB级 2 6例 (4 2 6 % ) ,ChildC组的患者术中凝血指标 (INR)的变化程度大于ChildB组 (P <0 0 5 )。与凝血有关的并发症中大出血 5例 (8 2 % ) ,肾衰 6例 (9 8% ) ,肝动脉血栓形成 5例 (8 2 % ) ,手术开展两阶段对比 ,第二阶段主要因限制了大量凝血药及血制品的使用 ,并发症明显减少。结论 掌握好不同时期、不同患者出血和血栓形成的平衡是防治肝移植围手术期出凝血功能障碍的关键 相似文献
119.
W Zhou 《中华耳鼻咽喉科杂志》1991,26(5):297-8, 318
Thirty-one cases with hypopharyngeal carcinoma and 4 cases with esophageal carcinoma in cervical segment were treated surgically from April 1984 to April 1989. In 6 cases, while preserving the larynx, the hypopharynx and the esophagus were resected and reestablished by the colon. In 29 cases, total laryngectomy was also performed and 4 had their hypopharynx and esophagus substituted by the colon and 25 by the stomach. According to UICC (1982), there were 11 cases in stage II, 15 in stage III and 9 in stage IV. The mean 3-year and 5-year survival rates were 62% and 32% respectively. In fatal cases, 4 cases had recurrences at the site of pharyngeal anastomoses and 11 had metastases in the neck. 相似文献
120.
Foley尿管气囊压迫治疗骶前静脉丛大出血的评价(附6例报告) 总被引:5,自引:0,他引:5
目的:评价直肠癌根治术中用Foley尿管气囊压迫治疗骶前静脉丛大出血(MPVP)的临床价值。方法:分析1995~2005年用Foley尿管气囊压迫治疗骶前静脉丛大出血6例的临床资料。结果:6例骶前大出血中全部用Foley尿管气囊压迫控制出血,术中出血量为800~1700mL,Foley尿管于术后4d拔除3例,5d1例,6d2例,均无再出血,会阴切口均一期愈合。结论:Foley尿管气囊压迫治疗骶前静脉丛大出血是一种简单安全有效的治疗方法。 相似文献