Comparative analysis of accumulation of chlorine e6 and hematoporphyrin derivatives in subpopulations of peripheral blood lymphocytes

We studied accumulation of porphyrin photosensitizers chlorine e6, hematoporphyrin, and their derivatives by different lymphocyte subpopulations. The intensity of staining of B lymphocytes and natural killer cells with photosensitizers was higher compared to T lymphocytes. T cell subpopulation differed by their ability to bind photosensitizers. Relative accumulation of dimethyl esters of chlorine e6 and hematoporphyrin in cells surpassed that of nonesterified porphyrins. Translated fromByulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 138, No. 8, pp. 180–184, August, 2004 Thisk work was supported by the Belarussian-Russian Foundation for Basic Research (grant NO. B02R-084).     

胃癌单克隆抗体临床诊断应用及与CP相关性的探讨

目的：研究胃癌单克隆抗体的表达及临床意义。方法：用本室制备的对胃癌有较强特异性的ＭｃＡｂＧＭＧ１：１Ｄ１－２对１５１例内窥镜活检组织压印片进行免疫组织化学检查。结果：用本法检查组织印片中的癌细胞以及具有癌细胞相关抗原的癌前病变组织,特异性强,反应灵敏,结果可靠,结论：如果与内窥镜和常规病理学检查联合应用,会提高早期癌的检出率,本文还就抗人胃癌ＭｃＡｂ与ＣＰ的相关性进行了初步探讨。     

Frequency of use and knowledge of the WHO-surgical checklist in Swiss hospitals: a cross-sectional online survey

Background

The WHO-surgical checklist is strongly recommended as a highly effective yet economically simple intervention to improve patient safety. Its use and potentially influential factors were investigated as little data exist on the current situation in Switzerland.

Methods

A cross-sectional online survey with members (N?=?1378) of three Swiss professional associations of invasive health care professionals was conducted in German, French, and Italian. The survey assessed use of, knowledge of and satisfaction with the WHO-surgical checklist. T-Tests and ANOVA were conducted to test for differences between professional groups. Bivariate correlations were computed to test for associations between measures of knowledge and satisfaction.

Results

1090 (79.1%) reported the use of a surgical checklist. 346 (25.1%) use the WHO-checklist, 532 (38.6%) use the Swiss Patient Safety Foundation recommendations to avoid Wrong Site Surgery, and 212 (15.7%) reported the use of other checklists. Satisfaction with checklist use was generally high (doctors: 71.9% satisfied, nurses: 60.8% satisfied) and knowledge was moderate depending on the use of the WHO-checklist. No association between measures of subjective and objective knowledge was found.

Conclusions

Implementation of a surgical checklist remains an important task for health care institutions in Switzerland. Although checklist use is present in Switzerland on a regular basis, a substantial group of health care personnel still do not use a checklist as a routine. Influential factors and the associations among themselves need to be addressed in future studies in more detail.

     

用改良消减法克隆类风湿性关节炎滑膜细胞中的高表达基因

0　引言　在类风湿性关节炎 (rheumatoid arthritis,RA)中 ,关节内滑膜细胞 (fibroblast- like synovial cells,FL S)的过度增生是造成关节软骨破坏和关节畸形的关键环节 ,为了研究滑膜细胞发生增殖的机制 ,我们采用赵忠良等人设计的改良消减杂交法[1 ] ,并以骨性关节炎 (osteoarthritis,OA)滑膜细胞为对照筛选 RA滑膜细胞中的高表达基因 .1　方法　将 RA滑膜细胞和 OA滑膜细胞分别设定为实验组和扣除组 ,用扣除组的 c DNAs杂交扣除掉实验组中与前者相同的 c DNAs,得到的就是目的产物 :实验组中高拷贝数的或独有的 c DNAs,也就是 …     

Silibinin prevents amyloid β peptide-induced memory impairment and oxidative stress in mice

Background and purpose:

Accumulated evidence suggests that oxidative stress is involved in amyloid β (Aβ)-induced cognitive dysfunction. Silibinin (silybin), a flavonoid derived from the herb milk thistle (Silybum marianum), has been shown to have antioxidative properties; however, it remains unclear whether silibinin improves Aβ-induced neurotoxicity. In the present study, we examined the effect of silibinin on the memory impairment and accumulation of oxidative stress induced by Aβ_25–35 in mice.

Experimental approach:

Aggregated Aβ_25–35 (3 nmol) was intracerebroventricularly administered to mice. Treatment with silibinin (2, 20 and 200 mg·kg⁻¹, once a day, p.o.) was started immediately after the injection of Aβ_25–35. Locomotor activity was evaluated 6 days after the Aβ_25–35 treatment, and cognitive function was evaluated in a Y-maze and novel object recognition tests 6–11 days after the Aβ_25–35 treatment. The levels of lipid peroxidation (malondialdehyde) and antioxidant (glutathione) in the hippocampus were measured 7 days after the Aβ_25–35 injection.

Key results:

Silibinin prevented the memory impairment induced by Aβ_25–35 in the Y-maze and novel object recognition tests. Repeated treatment with silibinin attenuated the Aβ_25–35-induced accumulation of malondialdehyde and depletion of glutathione in the hippocampus.

Conclusions and implications:

Silibinin prevents memory impairment and oxidative damage induced by Aβ_25–35 and may be a potential therapeutic agent for Alzheimer''s disease.     

三种阳离子脂质体介导血管内皮细胞基因转染效率的比较

目的:评价不同阳离子脂质体介导基因转染血管内皮细胞的转染效率。方法:实验于2006-12/2007-02在中山大学生化实验室及广州市创伤外科研究所完成。采用增强型绿色荧光蛋白基因为报告基因,分别采用Lipofectin、Lipofectamine、Dosper3种不同的阳离子脂质体为载体,转染人脐静脉血管内皮细胞。在24孔板中,每孔加入人脐静脉血管内皮细胞悬液(1×106个细胞),各孔分别加入3种不同阳离子脂质体增强型绿色荧光蛋白质粒复合物,分别于培养24,48h后用荧光显微镜及流式细胞仪测定增强型绿色荧光蛋白在细胞内的表达及转染效率。结果:3种不同阳离子介导的增强型绿色荧光蛋白基因转染的人脐静脉血管内皮细胞内均有绿色荧光蛋白表达,24h后明显,48h后达高峰。Dosper介导组绿色荧光细胞百分比明显高于Lipofectin介导组及Lipofectamine介导组,差异有非常显著意义(P<0.01)。结论:Dosper介导的血管内皮细胞基因转染效率较高,较适合作为血管内皮细胞的基因转染载体。