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101.
Incomplete antigenic cross-reactivity between platelets and megakaryocytes: relevance to ITP 总被引:6,自引:2,他引:4
Immune thrombocytopenias are usually associated with normal or increased numbers of megakaryocytes in the marrow. Therefore, the mechanism(s) responsible for the destruction of circulating platelets may not affect megakaryocytes in the same way. One of the possibilities which could account for the differential effect on the cells would be the development of antibodies to components of platelet membranes which are not exposed on the surface of all megakaryocytes. To investigate this possibility, a rabbit antiserum specific for mouse platelets was tested against fresh and cultured mouse megakaryocytes by indirect immunofluorescence. This antiserum cross-reacted with 46% of fresh murine megakaryocytes and 54% of cultured megakaryocytes. Phase- contrast microscopy revealed the reacting megakaryocytes to be fully granulated with irregular contours and in the process of releasing platelets. Nonreactive megakaryocytes demonstrated smooth contours and lacked morphological evidence of thrombocytopoiesis. Electron microscopy showed that only in megakaryocytes (MK) with an irregular contour had the demarcation membrane system (DMS) reached continuity with the plasma membrane. Ultrastructural analysis of megakaryocytes from patients with ITP showed approximately 25% to 50% of megakaryocytes without evidence of injury, whereas 50% to 75% had extensive damage. In undamaged cells, platelet territories had not yet reached the peripheral zone. The DMS of damaged megakaryocytes opened to the exterior elaborating platelets. The observations suggested that some platelet antibodies react only with megakaryocytes which have reached the stage of thrombocytopoiesis. Relevant target antigens may not be exposed on all megakaryocytes before cytoplasmic fragmentation occurs. 相似文献
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Altered expression of vascular endothelial cell (EC) surface antigens in response to irradiation is one of the early events of radiation-induced damage. Using flow cytometry, we investigated the immunocytochemical reactivity of a blind panel comprising 87 mAbs submitted to the endothelial section of the 6th International Workshop on Human Leukocyte Differentiation Antigens with irradiated and resting human dermal microvascular endothelial (HDME) and EA cell lines. Monolayers of irradiated cells received a single 5 Gy dose of 72 h prior to staining but were otherwise treated the same as resting cells. For comparative purposes we have also examined the immunohistochemical reactivity of the mAb panel with EC in ovarian tumour, Wilms' tumour and human placenta. In the flow cytometry experiments 42 and 44 mAbs stained HDME and EA cells respectively and while no antibody stained irradiated but not unirradiated cells, upregulation was seen for CD31, CD34, CD141 and CD146 in irradiated cells. The upregulation of thrombomodulin (CD141) is noteworthy since it is a marker of EC damage and thus may be a useful reagent in investigations of vascular injury. Comparison with tissue staining showed that 21 mAbs were reactive with at least one tissue but not with either EA or HDME cells. Nine mAbs showed no cross reactivity with tissue and of these one reacted with EA cells only. 相似文献
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Partial chimerism after T-cell-depleted allogeneic bone marrow transplantation in leukemic HLA-matched patients: a cytogenetic documentation 总被引:1,自引:1,他引:1
Bertheas MF; Maraninchi D; Lafage M; Fraisse J; Blaise D; Stoppa AM; Michel G; Brizard CP; Gaspard MH; Novakovitch G 《Blood》1988,72(1):89-93
We evaluated serially by cytogenetics the blood and marrow chimerism of 38 leukemic recipients of HLA-matched bone marrow transplants (BMT) who were prepared by high doses of alkylating agents and fractionated total- body irradiation (2.2 Gy X 5). Donor or host mitoses were identified by examination of sex chromosomes in 32 patients or by evaluation of the polymorphism of other chromosomes after specific banding in six patients. Twenty-four patients were recipients of untreated BMT, and 14 were recipients of T-cell-depleted BMT. In the 24 patients who received untreated BMT, all showed successful engraftment, and only three had a transient mixed chimera. In the 14 recipients of T-cell-depleted BMTs, four rejected their grafts, and seven had mixed chimeras; these mixed chimeras were more frequent in blood lymphocytes than in marrow cells and could be detected up to 26 months after BMT. This high frequency of partial chimerism after T-cell-depleted BMT by comparison with a control group suggests that the donor's T cells play an important role in the eradication of host residual hematopoiesis after allogeneic BMT. 相似文献
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