Immunohistochemical study of cells of invasive cancer of the vaginal part of the cervix uteri]

Immunohistochemical study of cervical carcinoma used EE21-06d monoclonal antibodies which identify five cytokeratin polypeptides inherent in the squamous epithelium. PAP-method and reaction of immunofluorescence were employed. Initial stages of squamous cell carcinoma invasion were characterized by bleaching or complete cell discoloring of most tumor cells. However, in deeply invading tumors, the share of intensively stained cells was markedly increased. The results point to expression of different cytokeratins or cell clones replacement with tumor progression. The peculiarities of cytokeratin distribution may serve to determine the degree of invasion and differentiation of tumor cells.     

The characteristics of the hemagglutinin from persistent variants of the influenza virus A/Victoria/35/72 (H3N2)

Electrophoresis in polyacrylamide gel of the reference influenza A/Victoria/35/72 (H3N2) virus and its persisting variants (PV) showed that the PV isolated on the 158th day from the moment of persistence modelling (PV158) had mutation in the gene of hemagglutinin (HA). This mutation is manifested by incomplete HA synthesis at 40 degrees C and increase of mobility of the light HA subunit (HA2). Analysis of nucleotide sequence of the greater part of HA gene of PV158 virus revealed 5 nucleotide substitutions four of which were significant. Three substitutions were found in Hal: 219 (Ser----Phe), 220 (Arg----Gly), 226 (Leu----Gln) and one in HA2: 156 (Thr----Asn). The importance of these mutations in the determination of the PV phenotype is discussed.     

Glycosylated hemoglobin (HbA1c) in blood in impaired tolerance to glucose and sugar diabetes

Isoelectric focussing in borate-poliol system is employed for measuring the blood glycosylated hemoglobin (HbA1c) levels at the treatment and prophylaxis institutions in Yalta. Examinations of normal subjects and patients with disordered glucose tolerance and apparent diabetes mellitus have revealed a significant elevation of HbA1c level in the patients with impaired glucose tolerance vs. normal subjects; these levels have been virtually the same in the diabetics and patients with impaired glucose tolerance. Over the course of health resort treatment the diabetics develop negligible changes in the HbA1c levels, paralleled by a significant reduction of glycemia on an empty stomach.     

Interaction of quinidine, lidocaine, tricaine and pyromecaine with heparin

In the experiments on rabbits it was shown that quinidine administered intravenously in a dose of 5 mg/kg exerts no effect on the blood coagulability but decreases the degree and duration of the anticoagulant effect of heparin. Lidocaine, trimecaine and pyromecaine at the same dose alter neither the blood coagulability nor the anticoagulant effect of heparin.     

Organ-sparing treatment of finger injuries caused by a punching]

There have been studied 1037 cases of hand and finger punch injuries, observed in 786 victims. An analysis of the trauma causes allowed to carry out the complex of preventive measures. The efficiency of these measures is testified by 3-fold decrease of occupational traumatism at Lysvensk metal works during 5 recent years. It has been stated that 496 (52.6%) of 945 finger and hand wounds were tissue avulsion and crush. These damages are typical ones in case of finger injuries with a punch. There have been analysed the results of treatment of 341 patient with scalped wounds, open fractures, finger and hand avulsions and crushes (482 wounds). It has been stated that careful surgical treatment of a wound with edge dissection, removal of damaged fatty tissue and free fractured fragments is a reliable method of infectious complication prophylaxis when the wound has been stitched without intention and the skin defects closed by means of the first skin plasty. The first skin plasty has been used for treatment of 62 wounds. Complete graft survival has been noted in 70.9% cases. In 85% of cases there have been obtained good functional results. A special section of sparing treatment presents finger replantation in case of their avulsion. Microsurgical technique has been applied with 9 patients. There have been replanted 13 fingers and 1 hand (survived 10 fingers and the hand). There have been revascularized 3 fingers (2 of them have survived).     

Role of ultrasound dopplerography with acute captopril test in assessment of renal hemodynamics in chronic glomerulonephritis

AIM: To examine blood flow in renal and intrarenal arteries and its changes in the acute pharmacological test with captopril in patients with chronic glomerulonephritis (CGN). MATERIAL AND METHODS: Renal circulation was studied in 50 patients with CGN using ultrasound dopplerography (USDG) of renal vessels on the unit GE Logiq 400 CL PRO Series. The velocity and indices of peripheral blood resistance in the major renal artery (RA) and in intrarenal arteries were estimated. In 26 patients the blood flow was studied again after intake of 50 mg captopril. RESULTS: Poor renal blood flow was registered in cortical parenchyma in 36% CGN patients (with chronic renal failure in 75%). Multifactorial regression analysis has demonstrated that only blood creatinine was independently related with slowing down of the blood flow at the level of RA and intrarenal arteries. Morphological index of activity correlated with resistance indices while a high sclerosis index correlated with blood flow slowing. Older patients had higher resistance indices. Captopril significantly accelerated blood flow and insignificantly changed indices of peripheral resistance including those in CRF patients. CONCLUSION: Poor blood flow in the cortical layer of renal parenchyma in CGN, according to USDG, occurs rather frequently and was associated with CRF and older age of the patients. Blocking of renin-angiotensin system at the level of angiotensin II formation improves renal blood flow in most of the patients.