Percutaneous drainage access: a simplified coaxial technique

We describe an access technique that we have used in 150 nephrostomy and biliary drainage procedures and for access to some abscesses and viscera. The system provides safe coaxial access with a 22-gauge removable hub needle, which then acts as a guide wire and is replaced by an 18-gauge cannula. A major advantage is that only one guide wire is used (0.038-inch) for the entire drainage procedure. No significant complications have occurred to date with this method.     

反复种植失败的相关对策新进展

辅助生殖技术的迅速发展使众多不孕症患者借助体外受精-胚胎移植(IVF-ET)及其衍生技术获得了后代。然而很大一部分妇女经历多次优质胚胎移植亦不能获得妊娠,反复种植失败(RIF)已经成为阻碍妊娠率进一步提高的瓶颈问题,且日益受到生殖医学界的广泛关注。就目前的条件而言,对RIF患者给予药物或者机械操作以提高子宫内膜容受性,行宫腹腔镜检查排除宫腔及盆腔病变以改善胚胎种植环境,通过辅助孵化、选择性囊胚移植、植入前胚胎遗传学筛查、共培养等技术提高胚胎着床能力都有可能改善和提高其种植率及妊娠率。RIF成为了我们亟待解决的问题,现综述近年有关反复种植失败的相关对策新进展。     

宫腹腔镜联合手术诊治不孕症150例分析

目的:探讨宫腹腔镜联合手术在诊治不孕症中的应用。方法:对150例不孕症患者行宫腹腔镜联合手术,对不孕症病因进行诊断,同时行治疗。结果:盆腔粘连和输卵管阻塞是不孕症的主要原因。子宫内膜息肉和正常盆腔占次要比例。子宫内膜异位症和多囊卵巢也是主要病因。150例同时行宫腹腔镜输卵管通液和各种疾病的治疗。联合手术后妊娠率为48.8%。结论:腹腔镜联合手术,在一次麻醉下,可以对不孕的原因全面评价和明确诊断,在诊断同时进行治疗,对不孕症的诊断和治疗有重大的意义,值得推广。     

bcl-2 transgene expression promotes survival and reduces proliferation of CD3-CD4-CD8- T cell progenitors

Proliferative expansion and apoptotic cell death play prominent roles in T cell development. The molecular control of cell cycle progression and apoptosis appear to be inter-connected since the Bcl-2 protein can inhibit apoptosis and slow cell cycle progression in cortical thymocytes and mature T cells, particularly during the transition from the quiescent state into the cell cycle. Here the impact of bcl-2 transgene expression on CD3-CD4-CD8- T cell progenitors was assessed. Bcl-2 enhanced the survival of these progenitors at all of the four major differentiation stages, CD25- CD44+ (pro-T1), CD25 + CD44+ (pro- T2), CD25 + CD44- (pro-T3) and CD25-CD44- (pro-T4). However, it reduced cell cycling and slowed turnover only in the pro-T4 subset. From an analysis of bcl-2 transgenic mice expressing a TCR transgene or bearing a mutation in the scid or rag-1 gene we conclude that Bcl-2 inhibits proliferation only of T cell progenitors that are activated via the pre- TCR, not those stimulated via c-Kit and the IL-7 receptor.      

Inactivation of purified human alpha 2-antiplasmin and purified human C1 inhibitor by synthetic fibrinolytic agents

3-Hydroxypropyl flufenamide (Flu-HPA) is one of a series of flufenamic acid derivatives that enhances blood clot lysis in vitro. Studies of possible mechanisms of action of Flu-HPA were undertaken. The profibrinolytic activity of Flu-HPA in clot lysis assays was found to be dependent on plasminogen. The influence of Flu-HPA on the ability of purified alpha 2-antiplasmin to inhibit purified plasmin was studied. Plasmin activity was determined using 125I-fibrin plates or the spectrophotometric tripeptide substrate, Val-Leu-Lys-paranitroanilide. At Flu-HPA concentrations greater than 1 mM, the inhibitory activity of alpha 2-antiplasmin was abolished in a time-dependent and concentration- dependent manner. The influence of Flu-HPA on the ability of purified Cl inhibitor to inhibit purified plasma kallikrein and beta-Factor XIIa was also studied. Cl inhibitor activity was abolished by Flu-HPA at concentrations greater than 2 mM. Notably, Flu-HPA up to 60 mM did not affect the amidolytic activities of plasmin, kallikrein, or beta-Factor XIIa. Flu-HPA did not release enzyme activity from preformed complexes of either alpha 2-antiplasmin and plasmin of Cl inhibitor and kallikrein. A water-soluble derivative of flufenamic acid, N-flufenamyl- glutamic acid, also inactivated alpha 2-antiplasm and Cl inhibitor. This inactivation was shown to be reversible. These results indicate that synthetic fibrinolytic compounds such as flufenamic acid derivatives may promote fibrinolysis by directly inactivating alpha 2- antiplasmin and Cl inhibitor.     

肛门镜压迫下内痔注射法治疗Ⅱ、Ⅲ期内痔的临床疗效

为探讨新型肛门镜压迫下内痔注射法治疗Ⅱ、Ⅲ期内痔的临床疗效,本研究采用多中心随机对照方法,选取200例Ⅱ、Ⅲ期内痔患者分为试验组及对照组,各i00例,试验组采用新型肛门镜压迫下内痔注射法治疗,对照组采用传统消痔灵注射法治疗,观察两组疗效及不良反应。结果显示,试验组治愈84例,好转12例,无效4例,治愈率为84．0％;对照组治愈30例,好转58例,无效12例,治愈率为30．0％。试验组治愈率明显高于对照组,P〈0．01。两组术后第3天出血治愈率差异无统计学意义,P〉0．05;但试验组术后痔核脱出、肛门坠胀治愈率明显高于对照组,P〈0．01。结果表明,肛门镜压迫下内痔注射法治疗Ⅱ、Ⅲ期内痔疗效确切,与传统消痔灵注射法比较,在治疗内痔脱出方面具有更高的临床实用价值。？     

肝脏增强CT扫描与MRI在肝癌诊断中的研究价值

目的探讨增强CT扫描与MRI在肝癌诊断中的价值。方法回顾性分析2014年5月至2016年3月在本院进行影像检查并经手术病理确认的60例肝癌患者的临床资料和影像资料,同时对患者癌变部进行归类分析,并通过两种影像检查方法的确诊率来评判两种检查方式的价值。结果60例中,巨块型占总数,结节型占总数54.5%,小肝癌例数占总数30.3%。增强CT对肝癌患者的总检出率为91.2%,MRI对肝癌患者的总检出率为92.3%。两者无明显统计学差异。结论在肝癌的确诊方面,MRI和增强CT扫描均具有较高的精度,可以作进一步推广应用。     

唐山地区药用植物资源及其可持续开发利用研究

目的:调查唐山地区药用植物资源的种类与分布,为药用植物资源的开发利用提供依据。方法:深入各代表性地域进行药用植物资源调查,采集标本,鉴定标本,考证文献资料,调查在民间的应用等。结果:唐山地区有药用植物113科、301属、382种,其中蕨类植物7科8属11种;裸子植物5科6属8种;单子叶植物9科36属42种。双子叶植物92科251属321种。结论:唐山地区药用植物资源丰富,具有较好的开发利用前景。