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排序方式: 共有633条查询结果,搜索用时 15 毫秒
51.
A comparison of neurological, metabolic, structural, and genetic evaluations in persons at risk for Huntington's disease 总被引:6,自引:0,他引:6
S T Grafton J C Mazziotta J J Pahl P St George-Hyslop J L Haines J Gusella J M Hoffman L R Baxter M E Phelps 《Annals of neurology》1990,28(5):614-621
We compared four diagnostic data sets for the assessment of individuals at risk for Huntington's disease. Fifty-four chorea-free persons were evaluated by neurological examination, positron emission tomography measurement of glucose metabolism, radiographic computerized tomographic measurement of caudate size, and genetic testing at the polymorphic DNA loci D4S10, D4S43, and D4S125. Twelve (22%) persons had abnormal caudate metabolism, 6 (11%) had subtle abnormalities of motor control, and 7 (13%) had computed tomographic evidence of caudate atrophy, compared with an expected gene frequency of 34% for this population. In 20 persons with unambiguous genetic test results or the subsequent phenotypic expression of Huntington's disease (chorea), there was a greater sensitivity of the positron emission tomographic measurement of caudate metabolism (75%) relative to computed tomography (33%) or the clinical examination (17%) for the determination of a subpopulation of probable Huntington's disease gene carriers. Hypometabolism of the putamen and globus pallidus, and hypermetabolism of the precentral gyrus were also associated with a high probability of carrying the Huntington's disease gene. The findings support the hypothesis that abnormalities of cerebral metabolism precede clinical or structural (computed tomographic) abnormalities in gene-positive individuals at risk for Huntington's disease. 相似文献
52.
R D Siewers J Ettedgui E Pahl T Tallman P J del Nido 《The Annals of thoracic surgery》1991,52(3):608-13; discussion 613-4
Hypoplasia of the transverse aortic arch of various degrees of severity is commonly seen in infants who have coarctation of the aorta. It is more often present when the coarctation is associated with intracardiac lesions that diminish or limit forward flow in the ascending aorta and promote right to left flow through an arterial duct. The increased frequency of surgical treatment of infants with complex coarctation, which is in part related to the ability to stabilize their condition with prostaglandin E1, has posed the question of the potential for growth and development of the originally hypoplastic aortic arch after conventional repair of aortic coarctation. Review of our experience with transverse aortic arch hypoplasia, found in 33 (32%) of 102 infants undergoing coarctation repair by subclavian flap aortoplasty or classic resection and end-to-end anastomosis, revealed excellent growth of the transverse arch after repair in all patients available for linear follow-up. The currently proposed extended arch repair should be reserved for the small group of infants with transverse aortic arch to ascending aorta diameter ratios (arch indices) of less than 0.25. 相似文献
53.
54.
Pahl A Zhang M Török K Kuss H Friedrich U Magyar Z Szekely J Horvath K Brune K Szelenyi I 《The Journal of pharmacology and experimental therapeutics》2002,301(2):738-746
By virtue of its binding to cyclophilin, the cellular receptor for cyclosporine (CsA), we could identify a new compound D-43787 [N-[(1-tert-butyloxycarbonyl)-indolin-2-(S)-carbonyl]-indolin-2-(S)-carbonacid-[N-epsilon-benzyloxycarbonyl)-2-(S)-lysin methylester]-amide] exhibiting immunomodulating properties. It inhibited cell proliferation induced by 12-O-tetradecanoylphorbol-13-acetate (TPA)/ionomycin and anti-CD3/CD28 with an IC(50) of 0.3 microM. The protein phosphatase calcineurin, which is the target of the CsA-cyclophilin complex, is not inhibited by D-43787. It inhibited T helper cell (Th) 2 cytokines interleukin (IL)-4, -5, and -13 more effectively than the Th1 cytokine interferon (IFN)-gamma in human primary T cells. The IC(50) for IL-5 and IL-13 in TPA/ionomycin-stimulated peripheral blood mononuclear cells (PBMC) is 0.7 +/- 0.1 and 0.5 +/- 0.1 microM, respectively, whereas the IC(50) for IFN-gamma is 2.0 +/- 0.4 microM. When PBMC were stimulated with anti-CD3/CD28, the IC(50) for IL-4, -5, and -13 were 1.5 +/- 0.2, 1.8 +/- 0.2, and 1.9 +/- 0.4 microM, respectively. IFN-gamma was only partially inhibited under these conditions. This effect was even more pronounced in pure CD4(+) T cells. Pretreatment of human monocytes with D-43787 inhibited lipopolysaccharide-induced proinflammatory cytokines IL-6 and TNFalpha with an IC(50) of 1.2 +/- 0.1 and 4.7 +/- 0.9 microM, respectively. In vivo, D-43787 potently inhibited late-phase eosinophilia in actively sensitized and challenged guinea pigs (10 mg/kg, i.p.: 51%) and Brown-Norway rats (1 mg/kg, intrapulmonary: 66% 30 mg/kg, i.p.: 50%). In adjuvant-induced arthritis, D-43787 (10-40 mg/kg, b.i.d., i.p.) dose dependently reduced edema development on both hind paws. The potency of D-43787 was comparable with that of indomethacin and dexamethasone. In conclusion, we characterized a novel Th2 selective immunosuppressive drug with possible anti-asthmatic/anti-inflammatory effects. Its mode of action is distinct from that of CsA. 相似文献
55.
K G Chandy M Pahl N D Vaziri S Gupta 《Journal of clinical & laboratory immunology》1985,17(3):119-124
The acute effect of dialysis on T-lymphocyte responses was studied in 11 patients with end-stage renal disease (ESRD). The in-vitro mitogenic response to concanavalin A and pokeweed mitogen was decreased (p less than 0.05) after single passage of blood through the dialyzer, accompanied by a reduction in the proportion of monoclonal antibody-defined total T lymphocytes (Leu 1+ cells) (p less than 0.01), an increase in the percentage of monoclonal antibody-defined monocytes (M 2+ cells), and a decrease in interleukin 2 (IL-2) production (p less than 0.05). Depletion of adherent cells from mononuclear cells isolated from blood after single passage through the dialyzer restored the mitogenic responses to normal levels. Post dialysis mitogenic responses were comparable to pre-dialysis mitogenic responses although IL-2 production (p less than 0.05), and the proportion of T lymphocyte (Leu 1+ cells) remained depressed (p less than 0.01). Cumulative effects of long-term intermittent hemodialysis may contribute to the impaired immunity and the increased frequency of infections and neoplasms in patients with end-stage renal disease. 相似文献
56.
Sulgi Kim Hanna E. Abboud Madeleine V. Pahl John Tayek Susan Snyder James Tamkin Harry Alcorn Jr. Eli Ipp Cynthia C. Nast Robert C. Elston Sudha K. Iyengar Sharon G. Adler 《Clinical journal of the American Society of Nephrology》2010,5(6):1072-1078
Background and objectives: Diabetic nephropathy (DN) is a multifactorial complication characterized by persistent proteinuria in susceptible individuals with type 1 and type 2 diabetes. Disease burden in people of Mexican-American descent is particularly high, but there are only a few studies that characterize genes for DN in this ethnic group. Two genes, carnosine dipeptidase 1 (CNDP1) and engulfment and cell motility 1 (ELMO1) previously showed association with DN in other ethnic groups. CNDP1 and ELMO1 were examined along with eight other genes that are less well characterized for DN in a new study of Mexican-Americans.Design, setting, participants, & measurements: The target sample was patients of Mexican-American ancestry collected from three centers: 455 patients with DN and 437 controls with long-term diabetes but no incident nephropathy. Forty-two, 227, and 401 single nucleotide polymorphisms (SNPs) in CNDP1, ELMO1, and the other eight genes, respectively, were examined.Results: No region in CNDP1 or ELMO1 showed significant P values. Of the other eight candidate genes, an association of DN with a SNP pair, rs2146098 and rs6659783, was found in hemicentin 1 (HMCN1) (unadjusted P = 6.1 × 10−5). Association with a rare haplotype in this region was subsequently identified.Conclusions: The associations in CNDP1 or ELMO1 were not replicable; however, an association of DN with HMCN1 was found. Additional work at this and other loci will enable refinement of the genetic hypotheses regarding DN in the Mexican-American population to find therapies for this debilitating disease.Diabetic nephropathy (DN) is the main cause of ESRD in the United States (1). The disease burden in people of Mexican-American descent is particularly high (1), but there are only a limited number of studies that have characterized genes for DN in this ethnic group. Recently, two genes, carnosine dipeptidase 1 (CNDP1) and engulfment and cell motility 1 (ELMO1), were reported to be associated with DN (2–5). Janssen et al. (4) reported an association between DN and a microsatellite marker, D18S880, in CNDP1 among type 1 and type 2 diabetic patients from four different countries, and Freedman et al. (2) reported its replication among type 2 diabetic Caucasian patients. Shimazaki et al. (5) reported an association in the Japanese population between DN and ELMO1, which includes rs741301 as the most significant single nucleotide polymorphism (SNP).Here, we study ten candidate genes for their association with DN in the Mexican-American population. We attempt to replicate the previous associations of CNDP1 and ELMO1 with a sample size that is similar or greater than previously used (2–5). In addition, we study the following eight genes, which are good biologic candidates but have not been studied extensively: hemicentin 1 (HMCN1), complement factor H (CFH), α-2Heremans-Schmid-glycoprotein (AHSG), caspase 3 (CASP3), heat shock 70-kD protein 1A (HSPA1A), heat shock 27-kD protein 1 (HSPB1), caspase 12 (CASP12), and heme oxygenase (decycling) 1 (HMOX1).HMCN1 was shown to be associated with change in calculated GFR (6), but its role in DN has never been examined. CFH is long known to play a role in atypical hemolytic uremia and membranoproliferative GN, but its involvement in DN has not been evaluated. AHSG is reported to be associated with type 2 diabetes and dyslipidemia, it inhibits insulin-induced tyrosine phosphorylation of insulin receptor substrate-1 (7), and it has been identified as a marker of acute kidney injury (8). Its serum concentration is increased in nondialyzed patients with DN (9) and is low in patients with ESRD (10). High serum levels are associated with insulin resistance (11). HSPB1, also known as HSP27, is involved in the regulation of cell adhesion and invasion (12), regulates actin cytoskeleton turnover, and has anti-apoptotic and antioxidant properties in a wide variety of cells and tissues (13). A mutation in HSPB1 causing a variant of Charcot-Marie-Tooth disease is associated with the development of focal and segmental glomerulosclerosis (14). HMOX1, also known as HO-1, provides antioxidant adaptive functions in response to renal injury (15) and is associated with the degree of renal failure in DN (16). CASP3 and CASP12 mediate apoptotic cell death and were chosen as candidate genes because of their relevance to DN (17,18). Finally, HSPA1A was chosen because of its cellular protectant role in the unfolded protein response (19).Our study aimed to replicate the previous association of the two genes with DN and/or discover new associations at the other eight genes of biologic importance by contrasting the genotype frequencies of SNPs in these ten genes between cases and controls after allowing for relevant covariates. 相似文献
57.
Serkan Erkan Chunhui Wu Amir A. Mehbod Brian Hsu Douglas W. Pahl Ensor E. Transfeldt 《European spine journal》2009,18(6):807-814
Single level axial lumbar interbody fusion (AxiaLIF) using a transsacral rod through a paracoccygeal approach has been developed
with promising early clinical results and biomechanical stability. Recently, the transsacral rod has been extended to perform
a two-level fusion at both L4–L5 and L5–S1 levels (AxiaLIF II). No biomechanical studies have been conducted on multilevel
fusion using the AxiaLIF technique. In this study, the biomechanics of L4–S1 motion segments instrumented with the AxiaLIF
II transsacral rod was evaluated. Six human cadaveric lumbosacral spine segments from L4 to S1 were used (age ranges 46–74 years).
Unconstrained and non-destructive pure moments in axial torsion, lateral bending, and flexion extension were applied to each
specimen following intact, standalone AxiaLIF II, and AxiaLIF II with two posterior fixation options: facet screws and pedicle
screws with rods. Range of motion was calculated from the raw data collected with an optical motion tracking system. The two-level
transsacral rod was successfully inserted in all the specimens. At L4–L5 level in axial torsion (AT) and flexion extension
(FE), none of the surgical treatments showed statistically significant difference between the procedures (all P > 0.05) although facet screws and pedicle screws had higher stability on average. In lateral bending (LB), the two posterior
fixation techniques had significantly higher construct stability (P < 0.05) than the standalone rod. No significant difference was found between facet screws and pedicle screws (P = 0.821). At L5–S1 level in AT and LB, none of the surgical treatments were found to be statistically significant (all P > 0.05). In FE, standalone two-level transsacral rod had significantly higher range of motion (ROM) compared with the posterior
fixation techniques (P < 0.05). In conclusion, the standalone rod reduced intact ROM significantly. Supplementary fixations including facet screws
and pedicle screws are required to achieve higher construct stability for successful fusion. Further clinical studies are
essential to evaluate the practical success of this technique. 相似文献
58.
59.
Rives S Pahl HL Florensa L Bellosillo B Neusuess A Estella J Debatin KM Kohne E Schwarz K Cario H 《Haematologica》2007,92(5):674-677
Dominant mutations in the erythropoietin receptor (EPOR) gene account for only about 15% of cases of primary congenital erythrocytosis. To search for molecular alterations in patients with this disorder. Sixteen patients with Epo <10 mU/mL were studied, 3 were related. Analyses included EPOR and JAK2 gene sequencing, quantitative PRV-1 RT-PCR, and erythroid colony assays. A novel sporadic EPOR 1453G->A (Trp439Stop) mutation was detected. All familial cases, varied in phenotype, presented the EPOR 1414C->G (Tyr426Stop) mutation. JAK2 mutations are not involved in the pathogenesis of primary congenital erythrocytosis. 相似文献
60.
Iyengar SK Abboud HE Goddard KA Saad MF Adler SG Arar NH Bowden DW Duggirala R Elston RC Hanson RL Ipp E Kao WH Kimmel PL Klag MJ Knowler WC Meoni LA Nelson RG Nicholas SB Pahl MV Parekh RS Quade SR Rich SS Rotter JI Scavini M Schelling JR Sedor JR Sehgal AR Shah VO Smith MW Taylor KD Winkler CA Zager PG Freedman BI;Family Investigation of Nephropathy Diabetes Research Group 《Diabetes》2007,56(6):1577-1585
The Family Investigation of Nephropathy and Diabetes (FIND) was initiated to map genes underlying susceptibility to diabetic nephropathy. A total of 11 centers participated under a single collection protocol to recruit large numbers of diabetic sibling pairs concordant and discordant for diabetic nephropathy. We report the findings from the first-phase genetic analyses in 1,227 participants from 378 pedigrees of European-American, African-American, Mexican-American, and American Indian descent recruited from eight centers. Model-free linkage analyses, using a dichotomous definition for diabetic nephropathy in 397 sibling pairs, as well as the quantitative trait urinary albumin-to-creatinine ratio (ACR), were performed using the Haseman-Elston linkage test on 404 microsatellite markers. The strongest evidence of linkage to the diabetic nephropathy trait was on chromosomes 7q21.3, 10p15.3, 14q23.1, and 18q22.3. In ACR (883 diabetic sibling pairs), the strongest linkage signals were on chromosomes 2q14.1, 7q21.1, and 15q26.3. These results confirm regions of linkage to diabetic nephropathy on chromosomes 7q, 10p, and 18q from prior reports, making it important that genes underlying these peaks be evaluated for their contribution to nephropathy susceptibility. Large family collections consisting of multiple members with diabetes and advanced nephropathy are likely to accelerate the identification of genes causing diabetic nephropathy, a life-threatening complication of diabetes. 相似文献