重度蒸汽吸入性损伤犬伤后肿瘤坏死因子水平的改变及其意义

目的研究蒸汽吸人性损伤犬伤后血浆肿瘤坏死因子水平的改变及其与吸入性损伤呼吸循环功能受损程度的关系.方法取犬15条,以蒸汽致重度吸入性损伤,分别于伤前、伤后1h、2h、3h、4h观察有呼气相气道阻力(RE)、心率(HR)、心输出量(CO)、动脉血氧分压(PaO2)、血浆TNF水平;伤后4h致死动物,取肺做病理检查.结果伤后1hTNF、RE升高,HR、CO、PaO2下降;TNF与RE正相关(r=O.965,P<O.01),与CO负相关(r=-0.824,P<O.05).结论严重蒸汽吸入性损伤激发TNF分泌,这是诱发肺损伤及循环系统甚至全身器官功能障碍的重要原因.     

肌源性耳鸣的声导抗测试特征（附3例报告）

本文报道了１１例病例,并以３例典型病例就肌源性耳鸣的声导抗测试的基本方法和特征作了探讨。腭肌阵挛的幅度较大,在鼓室导抗图和声反射曲线上均可出现锯齿状波。镫骨肌阵挛在表情肌运动时可诱发,为持续性,镫骨肌声反射对其无影响或可掩盖之。鼓膜张肌阵挛与面部三叉神经受刺激有关,易疲劳,表现为一短暂的声顺变化波形。     

四项肿瘤标志物联合检测对PHC的临床评价

对原发性肝癌（PHC）81例;良性肝病58例;转移性肝癌11例;正常对照20例,进行血清标志物甲胎蛋白（AFP）、AFP异质体、5′-核苷酸磷酸二酯酶同工酶（5′-NPD）、唾液酸（SA）联合检测。结果显示四项标志物的组合能提高对PHC的诊断率（93.83%),同时还可用于小肝癌的诊断及PHC疗效观察及估计预后。     

Clonal growth of human acute leukemia cells in serum-free methylcellulose medium

Ｔｈｅｇｒｏｗｔｈｏｆｌｅｕｋｅｍｉａｉｎｓｅｍｉｓｏｌｉｄｃｕｌｔｕｒｅｉｎｖｉｔｒｏｗａｓｄｅｐｅｎｄｅｎｔｏｎｔｈｅｐｒｅｓ－ｅｎｃｅｏｆｅｘｏｇｅｎｏｕｓｓｅｒｕｍ．Ｓｅｒｕｍｉｓａｍｉｘ－ｔｕｒｅｃｏｎｔａｉｎｉｎｇｌａｒｇｅｎｕｍｂｅｒｏｆｕｎｉｄｅｎｔｉｆｉｅｄｃｈｅｍｉｃａｌｃｏｍｐｏｎｅｎｔｓａｎｄｕｎｉｄｅｎｔｉｆｉｅｄｂｉｏ－ｌｏｇｉｃａｌｌｙａｃｔｉｖｅｓｕｂｓｔａｎｃｅｓ［１］,ｗｈｉｃｈｐｒｏｖｉｄｅｔｈｅｎｕｔｒｉｅｎｔｓｎｅｃｅｓｓａｒｙｆｏｒｔｈｅｃｅｌｌｇｒｏ…     

抗A1试剂血清的研制

目的 :ABO亚型在临床输血中经常造成错定血型及输血反应 ,因而研制抗A1试剂血清是解决ABO亚型鉴定的主要途径。方法 :采用血型血清学特异性抗原抗体吸收实验。结果 :经大量反复实验 ,研制出特异性强 ,效价符合要求的抗A1试剂血清。结论 :抗A1血清是鉴定A亚型的专用试剂血清 ,对临床鉴定ABO亚型及安全输血有实用意义。     

Lysosomal dysfunction results in lamina-specific meganeurite formation but not apoptosis in frontal cortex

An inhibitor of cathepsins B and L was used to test if lysosomal dysfunction in cultured slices of rat frontal cortex induces pathological features that develop in the human cortex during aging and Alzheimer's disease (AD). Incubation for 6 days with N-CBZ-L-phenylalanyl-L-alanine-diazomethylketone (ZPAD) resulted in a massive proliferation of endosomes-lysosomes in all cortical layers. Slices additionally exposed to a washout of 4 days had numerous meganeurites, blister-like structures in the region of the axon hillock, in layer III but not in other cortical laminae. Meganeurites are a characteristic feature of the human frontal cortex after age 50 and are largely restricted to layer III. Tests for apoptosis were carried out at two intervals following meganeurite formation. TUNEL-labeled neurons were confined to layers II/III on the surface of the slices but there was no evidence for a ZPAD effect. In all, 6 days of lysosomal dysfunction reproduces characteristic effects of normal aging in neocortex without generating some key features of AD.     

Prostate cancer old problems and new approaches

Rates of prostate cancer (PCa) have increased so dramatically over the last decade that the age adjusted incidence rate for PCa is now greater than that any other cancer among men in the United States. This review, published as a three part series, provides a state-of-art assessment of the PCa problem in its divergent aspects.Part 1 covers epidemiology, incidence and progression. Several epidemiological studies have demostrated that first degree male relatives of men with PCa are at increased risk of developing the disease. Familial and genetic factors as well as medical, anthropometric, dietary, hormonal and occupational factors involved in PCa are discussed. Postmortem examination of the prostate in men without evidence of PCa documented a high frequency of adenocarcinoma. Latent disease occurred as early as the second decade of life. Although there is no significant difference in incidence between Caucasian and African-American males, high grade prostatic intraepithelial neoplasia (HGPIN) is higher in the latter group. While dietary fat, androgens and certain environmental factors may be determinants for PCa, the exact mechanism of tumorigenesis is still relatively unknown. The current thinking of the role of genomic instability, chromosomal alterations, tumor suppressor genes and the androgen receptor are explored.     

Studies on constituents of Korean basidiomycetes (L)

To find antitumor metabolites in Korean basidiomycetes, the shake-cultured mycelia of eight of the higher fungi were extracted with hot water and the extracts, after being partially purified, were subjected toin vivo antitumor test. When administeredi.p. at the dose of 30mg/kg/day for ten consecutive days into the female ICR mice, which had been implanted with 1×10⁵ cells of sarcoma 180 twenty-four hours before the first injection, the extracts ofAgaricus campestris, Lyophyllum decastes, Lyophyllum ulmarium, Armillaria tabescence andCalvatia exipulitormis respectively showed inhibition ratios of 64.1%, 65.4%, 60.0%, 53.0% and 49.3%. These five species were selected for further study, whereas the extracts ofPhallus impudicus, Coprinus comatus andPholiota squarrosa which showed the inhibition ratios of 31.2%, 33.5% and 19.0% were discontinued.     

14例大疱性表皮坏死松解型药疹治疗体会

报告14例大疱性表皮坏死松解型药疹的治疗。以磺胺类药和解热镇痛药引起者居多,各4例。皮损面积按九分法计算,皮损面积80％以上者9例,80％以上者5例,治愈9例,死亡5例,我们认为早期使用足够剂量皮质激素类药物是有效措施。病程中注意防治感染,给予足够的液体以防止水电解质失平衡,局部治疗使用本院中药“五黄油”。