章念伟治疗慢性肾小球肾炎经验

慢性肾小球肾炎是一种变态反应性疾病,是慢性肾衰竭最常见的原发病,因此,及时治疗慢性肾炎,对阻止或延缓病情的发展、提高患者的生活质量至关重要。章念伟主任医师提出治疗慢性肾小球肾炎当以祛湿为主,活血化瘀,慎用补虚,平和用药,适时守方,于临床治疗可获得较好的疗效。     

磷酸化C-JUN不参与谷氨酸诱导的小脑颗粒神经元凋亡

观察磷酸化C-JUN与谷氨酸诱导的小脑颗粒神经元凋亡的关系.在培养的小脑颗粒神经元建立谷氨酸凋亡模型;采用MTT法分析细胞存活率,相差显微镜观察形态学,DNA凝胶电泳法分析细胞凋亡和原位细胞荧光免疫组织化学法检测磷酸化C-JUN.结果显示,谷氨酸诱导大鼠小脑颗粒神经元细胞体积缩小,突触断裂、消失,DNA电泳呈典型的"梯状"条带;谷氨酸处理24 h后细胞存活率为28.6 %±5.2 %.神经元在谷氨酸处理5,30 min及1,2,4,8,16和24 h后均未检测到有磷酸化C-JUN阳性细胞,与去极化组(25 mmol/L KCl)相同.而复极化组(5 mmol/L KCl)则在30 min检测到大量的磷酸化C-JUN阳性细胞,4 h荧光最强并持续.处理4 h后,400 倍荧光显微镜下,复极化组、去极化组和谷氨酸组的磷酸化C-JUN阳性细胞数分别为124 ±17,8±3,5±3.上述结果提示,谷氨酸诱导小脑颗粒神经元凋亡,磷酸化C-JUN不参与谷氨酸诱导的大鼠小脑颗粒神经元凋亡.     

磷酸化C—JUN不参与谷氨酸诱导的小脑颗粒神经元凋亡

观察磷酸化 C- JUN与谷氨酸诱导的小脑颗粒神经元凋亡的关系。在培养的小脑颗粒神经元建立谷氨酸凋亡模型 ;采用 MTT法分析细胞存活率 ,相差显微镜观察形态学 ,DNA凝胶电泳法分析细胞凋亡和原位细胞荧光免疫组织化学法检测磷酸化 C- JUN。结果显示 ,谷氨酸诱导大鼠小脑颗粒神经元细胞体积缩小 ,突触断裂、消失 ,DNA电泳呈典型的“梯状”条带 ;谷氨酸处理 2 4 h后细胞存活率为 2 8.6%± 5.2 %。神经元在谷氨酸处理 5,30 min及 1 ,2 ,4,8,1 6和 2 4 h后均未检测到有磷酸化 C- JUN阳性细胞 ,与去极化组 ( 2 5mmol/L KCl)相同。而复极化组 ( 5mmol/L KCl)则在 30 min检测到大量的磷酸化 C- JUN阳性细胞 ,4h荧光最强并持续。处理 4h后 ,40 0倍荧光显微镜下 ,复极化组、去极化组和谷氨酸组的磷酸化 C- JUN阳性细胞数分别为 1 2 4± 1 7,8± 3,5± 3。上述结果提示 ,谷氨酸诱导小脑颗粒神经元凋亡 ,磷酸化 C- JUN不参与谷氨酸诱导的大鼠小脑颗粒神经元凋亡。     

慢性非细菌性前列腺炎/慢性盆腔疼痛综合征患者前列腺液中炎症因子差异表达的临床意义

Objective To investigate the role of inflammatory cytokines in the pathogenesis of chronic non-bacterial prostatitis/chronic pelvic pain syndrome (CAP/CPPS) patients. Methods The 38 cases with CAP/CPPS patients (18 cases of CAP and 20 cases of CPPS) and 20 cases of healthy controls were selected. The differential expressions of 40 kinds of inflammatory cytokines were detec-ted by antibody arrays in prostate fluid. Results The inflammatory cytokines which increased more than 1.5 times expression have been found. There were seven kinds in CAP including monocyte che-moattractant protein (MCP)-1, solution tumor necrosis factor receptor Ⅱ(s TNF R Ⅱ), platelet-de-rived growth faetor-BB (PDGF-BB), interleukin (IL)-β, IL-11、IL-6、MCP-2 and five kinds in CPPS groups including MCP-1、PDGF-BB、MCP-2、s TNF R Ⅱ、It-11 respectively, compared with healthy control group. The cluster analysis results showed that protein expression of Monocyte chemoattrac-tant protein 1 (MCP-1)and platelet-derived growth factor BB (PDGF-BB) were significantly increased in CAP (3.47 and 2.07 times) and CPPS (2.25 and 2.19 times) compared with healthy control group and were the final polymerization of inflammatory cytokines. The protein expression of interleukin 1 β (IL-1 β), MCP-1 and soluble tumor necrosis factor Ⅱ (s TNF R Ⅱ) in CAP group was increased more than 1.85,1.55,1.67 times compared with CPPS group. Conclusions Elevated expression of inflammatory cytokines may play an important role in the course of CAP/CPPS disease. The extent of the inflammatory response of CAP was higher than CPPS. The inflammatory factors of MCP-1 and PDGF-BB could serve as a novel diagnostic marker.     

StarClose血管缝合器在冠状动脉介入术中临床应用的评价

目的评价StarClose血管缝合器在冠状动脉介入术中使用的安全性和有效性。方法 203例行冠状动脉介入治疗患者,其中男性110例,女性93例;年龄44～75岁,平均年龄57.92岁。依据股动脉穿刺点止血方法分为StarClose血管缝合器组（n=101）和人工压迫止血组（n=102）,分别记录两组患者股动脉止血时间、下肢制动时间和相关并发症。结果缝合器组的止血时间、下肢制动时间及并发症发生率明显低于人工压迫组（P〈0.05）。结论 StarClose血管缝合器应用于临床具有良好的安全性和有效性。     

利用表面肌电信号评价肌肉疲劳的方法

表面肌电信号分析是评价局部肌肉疲劳有效的工具。过去由于受信号处理技术的限制,对肌肉疲劳的评价仅局限于等长、恒力收缩。由于适合于非平稳信号分析的频谱估计技术的发展,使得动态收缩条件下的肌肉疲劳评价得以实现,在工效学、康复医学和运动医学等方面开启了新的应用领域。本文论述了利用表面肌电信号评价肌肉疲劳,特别是在肌肉动态收缩期间评价肌肉疲劳的方法,为进一步探讨肌肉疲劳的发生机制提供理论依据。     

SEN病毒ORF2基因序列和抗原性的初步分析

目的对SEN病毒(SENV)开放阅读框(ORF)2基因进行序列测定分析和原核表达,并对表达蛋白进行抗原性分析。方法用聚合酶链反应(PCR)方法扩增SENVORF2基因,对该基因进行序列测定、系统进化分析,双酶切扩增产物与原核表达载体pRSETB构建成重组质粒,诱导表达后进行SDSPAGE和免疫印迹分析。结果该株SENVORF2与北京株(AY072045)核苷酸同源性为97%,氨基酸同源性为59%;与日本株(AB059352)同源性分别为90%和56%。含有SENVORF2质粒的菌株表达相对分子质量约为19×103的融合蛋白,免疫印迹证明该融合蛋白能与SENVDNA阳性血清发生特异反应。结论表达了158个氨基酸的SENVORF2原核表达蛋白具有一定的抗原活性。     

甲氧西林耐药葡萄球菌SCCmec研究

目的明确甲氧西林耐药金黄色葡萄球菌（MRSA）和溶血性葡萄球菌（MRSH）所包含的葡萄球菌染色体mec盒（staphylococcal cassette chromosome mec，SCC mec）类型，并比较两者的差异。方法分别收集本院住院患者临床标本中分离的60株MRSA和88株MRSH。共设计10对引物，采用PCR法分别扩增mec复合体和ccr复合体的各个特征序列，分析其SCCmec类型。结果60株MRSA均为Ⅲ型SCCmec；88株MRSH中7株为V型SCCmec，1株为Ⅲ型SCCmec，其余均为新的组合或是无法分型。结论SCCmec在MRSA和MRSH中分布特点差别较大，MRSH中可能存在较多新的型别。     

Role of angiotensin Ⅱ and JAK2 signal pathway in transdifferentation of renal tubular cells in mice after acute ischemic followed by reperfusion

Objective To investigate the effect of angiotensin (Ang) Ⅱ and its Janns-activated kinase-2 (JAK2) signal pathway in transdifferentiation of renal tubular cells under the challenge of acute ischemic reperfusion injury.Methods Models of acute ischemic reperfusion injury were established and the level of local Ang Ⅱ ,a key element of renin-angiotensin system (RAS),in kidney was measured using radioimmunity technique.The expression of α-smooth muscle actin (α-SMA),a phenotype of mesenchymal cells,was detected by RT-PCR and inununohistochemistry methods.Renal tubule cells ( NRK-52E) were cultured with various concentration of Ang Ⅱ ,followed by blocking of PD123319,Ang U receptor 2 antagonist,and AG490,an inhibitor of JAK2 signal pathway.Results Ang 0 of kidney tissue increased immediately after acute ischemic-reperfusion injury,in time dependent fashion.Expression of α-SMA in renal tubule cells was found at 48 hours after ischemic-reperfusion injury and in NRK-52E cells treated by high concentration of Ang Ⅱ and was dose and time dependent.The peak of α-SMA expression was seen after 30 minute treatment at the dose of 10-9'mol/L,which was interrupted by both of PD123319 and AG490.Conclusions Transdifferentiarion of renal tubular epithelial cells occurs under acute ischemic- reperfusion injury.Local renin-angiotensin system may play a role in the transdifferentiation of TEC through AT2 receptor and its JAK2 signal pathway.     

湖南侗族左扣手人群的手纹分析

目的　对湖南侗族左扣手人群的手纹分布特点进行研究 ,积累侗族群体遗传学与民族学资料。方法　用油墨拓印法捺印手纹 ,放大镜下观察分析。结果　侗族左扣手人的指纹分型特点是 :尺箕 >简斗 >双箕斗 >桡箕 >简弓 >帐弓 ;Ws型在各手指的出现率、掌褶纹类型的百分率、以及总指纹嵴数和a -b纹嵴线数存在明显的性差 (P <0 0 5或P <0 0 1) ;女性Ws和Lu的出现率、男性Ws和Wd在各指的分布 ,以及通贯型掌褶纹百分率与对照组存在明显差异 (P <0 0 5或P <0 0 1)。结论　湖南侗族左扣手人的斗型纹与掌褶纹的分布具有一定的特异性