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In this study, 25 men, referred to our clinic for diagnosis and therapy of infertility were included. All had enlarged prostates. They were given 10 sessions of prostatic massage during 3--4 weeks and the fluid expressed was analysed for citric acid. The hypertrophy was seen to recede in almost all cases. Citric acid concentrations fell in only 6/25 cases analysed. In all the others, values did not fall and remained relatively stable. There was no apparent relationship between reduction of prostatic volume and the pattern of citric acid secretion. 相似文献
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BERTUCCIO IBARRA PIGNATARO TOLEDO PAZ ARRIZURIETA MARTIN 《Acta physiologica (Oxford, England)》1998,164(1):107-114
Chronic renal failure (CRF) is accompanied by adaptive changes in electrolyte reabsorption in the thick ascending limb of Henle of surviving nephrons. To study the cellular mechanism of this adaptation, we measured intracellular cAMP in micro-dissected medullary thick ascending limb (mTAL) segments in rats with CRF. mTAL exhibited in CRF an increase of basal cAMP from 25.6 ± 10.0 in controls to 65.8 ± 11.3 fmol mm?1 tubule in CRF (P < 0.05). Vasopressin and calcitonin stimulated mTAL adenylate-cyclase in a dose-dependent manner in controls but failed to stimulate in CRF. Likewise, maximal stimulation with 10?3 M 3-isobutyl-1-methylxanthine (IBMX) plus 10?5 M forskolin increased cAMP in controls to 63.0 ± 16.0 but not in CRF, where maximal stimulated values remained at 63.1 ± 18.8 fmol mm?1 tubule (P NS). Alpha2-adrenoreceptor activation with clonidine at concentrations ranging from 10?8 to 10?6 M diminished cAMP production by 37% in CRF (P < 0.05), whereas no differences were found in controls. Thus, the basal intracellular cAMP is increased in rat mTAL in CRF. The finding that neither forskolin nor vasopressin were able to further augment intracellular cAMP would suggest that stimulatory pathways of the adenylate-cyclase system are activated in the basal state. However, mTAL cells in CRF seem to retain the response of normal epithelium to inhibitory pathways such as the one mediated by alpha2-adrenoreceptors. 相似文献
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DE PAZ GARNELO JA 《Acta pediátrica espa?ola》1958,16(190):suppl 81-134 concl
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Migration sedimentation technique as a predictive test for the fertilizing capacity of spermatozoa in an in-vitro fertilization programme 总被引:2,自引:0,他引:2
R. HAUSER Z. T. HOMONNAI G. F. PAZ H. YAVETZ A. AMIT J. B. LESSING M. R. PEYSER L. YOGEV 《International journal of andrology》1992,15(6):498-503
The migration-sedimentation technique (MST) has been proposed as a means of separating high quality motile spermatozoa. The present study was conducted in order to evaluate whether sperm performance following separation by MST predicts their fertilizing capacity in an in-vitro fertilization (IVF) programme. Ninety semen specimens were analysed for use in an IVF-embryo transfer (ET) programme. Each specimens was divided into two parts: one was processed in the IVF programme and was used after sperm swim-up separation for insemination of human ova. The other aliquot (0.2 ml) was separated by MST, and the sperm then characterized by their concentration, motility, degree of motility and morphology. Sperm characteristics after separation by MST were then correlated with the results of the IVF-fertilization rates. In 79 of 90 IVF-ET cycles, at least one oocyte was fertilized. All post-MST sperm characteristics were significantly higher in cycles with fertilizations compared to IVF cycles without fertilization. A larger percentage of the total motile spermatozoa were recovered after MST in semen specimens with fertilization, compared to semen specimens without fertilization (39.9 +/- 3.6 and 20.6 +/- 6.6%, respectively; P < 0.05). This value was correlated with the percentage of fertilized oocytes (r = 0.24; P < 0.02). More IVF cycles with fertilizations were recorded in cases in which the recovery of motile sperm was > 25% (P < 0.005), or when more than 1.5 x 10(6) motile spermatozoa were recovered after MST (P < 0.0001). As sperm characteristics after MST correlated significantly with their fertilizing capacity, the MST test could be used in evaluation of the fertilizing capacity of spermatozoa. 相似文献
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R. HAUSER J. B. LESSING D. SAMUEL H. YAVETZ M. R. PEYSER G. F. PAZ Z. HOMONNAI 《International journal of andrology》1994,17(2):74-77
Bilateral nonpalpable testes in the adult human are associated with testicular malignancy, infertility and other abnormalities. Investigation for localization of the testes is mandatory, and either orchidopexy or orchidectomy is indicated. Laparoscopy was performed in an azoospermic male with bilateral unpalpable testes. A diagnosis of intra-abdominal testes was made and bilateral orchidectomy was performed. Laparoscopy is recommended for diagnosis and precise localization, as well as for orchidectomy, thus avoiding open abdominal operation in cases of intra-abdominal testes. 相似文献
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