辽宁地区尿路结石患者结石成分及相关因素分析

目的分析辽宁地区泌尿系结石成分特点,为该地区尿路结石的防治提供依据。方法收集2016年1月至2017年5月沈阳市尿路结石治疗中心163例尿路结石患者的病历资料和结石样本,采用红外光谱进行结石成分分析。结果 163例尿路结石共检出8种成分,混合成分的结石占90.2%;含两种成分的结石占54.0%;上尿路结石占总数的81.6%。一水和二水草酸钙结石在输尿管中高发,而碳酸磷灰石结石在肾脏中高发;男性与女性患者比例为3.2∶1;不同成分结石发生率的性别比较,男性的尿酸结石(χ²=5.196,P=0.023)和女性的磷酸钙结石(χ²=9.717,P=0.002)与异性相比差异有统计学意义;尿路结石高发于50～70岁年龄段。结论辽宁地区的尿路结石患者中,混合结石占绝大多数,多发于上尿路的输尿管,结石成分以一水草酸钙、二水草酸钙和碳酸磷灰石为主,男性多于女性,中老年高发。     

舒他西林水解反应的研究

为指导合理用药,对舒他西林特性进行了研究。舒他西林是由氨苄西林和舒巴坦通过双酯键连接而成的口服β内酰胺抗生素前药,在中性及偏碱性条件下极易水解,水解时两个酯键的活泼性不同,通常优先形成氨苄西林和舒巴坦羟甲酯;在自发水解液中氨苄西林和舒巴坦之比约3∶1;产生的氨苄西林羟甲酯和舒巴坦羟甲酯经酯酶等进一步水解可释放出甲醛。     

Characterization of soluble glycoprotein D-mediated herpes simplex virus type 1 infection

Herpes simplex virus type 1 (HSV-1) entry into permissive cells involves attachment to cell-surface glycosaminoglycans (GAGs) and fusion of the virus envelope with the cell membrane triggered by the binding of glycoprotein D (gD) to cognate receptors. In this study, we characterized the observation that soluble forms of the gD ectodomain (sgD) can mediate entry of gD-deficient HSV-1. We examined the efficiency and receptor specificity of this activity and used sequential incubation protocols to determine the order and stability of the initial interactions required for entry. Surprisingly, virus binding to GAGs did not increase the efficiency of sgD-mediated entry and gD-deficient virus was capable of attaching to GAG-deficient cells in the absence of sgD. These observations suggested a novel binding interaction that may play a role in normal HSV infection.     

High frequency of IgE receptor FcεRIß variant (Leu181/Leu183) in Kuwaiti Arabs and its association with asthma

The presence of IgE receptor FcεRIβ polymorphism (181/183) was investigated in Kuwaiti asthmatic patients and controls using an allele refractory mutation screening (ARMS) test. The variant sequence (Leu181/Leu183) was detected in 72% (320/442) chromosomes analysed. Homozygous LL genotype was detected in 48% (46/96) asthmatic subjects compared to 31% (39/125) in non-asthmatics. In 11/52 families mothers of the asthmatic children were themselves asthmatic and 3/11 asthmatic mothers had homozygous LL genotype. 80% of the homozygous LL asthmatics showed a positive skin prick test (SPT) compared with 28% of non-asthmatics with the same genotype. In heterozygous NL asthmatics a positive SPT was found in 60% cases compared to 17% in non asthmatics with the same genotype. The incidence of hay fever (HF) and eczema (E) was also found to be higher in homozygous LL asthmatics compared with the non-asthmatics with the same genotype. This study reports a high prevalence of IgE receptor FcεRIβ variants in Kuwaiti Arabs compared with British, Australian and Austrian populations studied before. The association of Leu181/Leu183 variant with asthma in Kuwaitis underlines its significance as a risk factor in manifesting the clinical phenotype in this population.     

盐酸苯乙哌啶和dl-15甲基PGF2α甲酯对大鼠卵巢黄体细胞的影响

盐酸苯乙哌啶(R1132)10μg/ml或dl-15甲基PGF_2α甲酯(PG05)5或10μg/ml在体外能明显抑制黄体细胞对hCG的反应性,使孕酮分泌下降。假孕大鼠po R1132 10 mg/kg或Sc PG 05 5.1 mg/kg不影响卵巢孕酮分泌,合并给药后却能使其降低。R1132无抗孕酮作用。卵巢分泌孕酮减少可能是抗早孕的主要原因.假孕大鼠po R1132 50 mg/kg或sc PG050.5 mg/kg可抑制卵巢腺苷环化酶的活性.该酶可能是R1132或PG05在大鼠抗早孕作用的重要靶酶。     

去氢表雄酮硫酸酯钠盐对妊娠大鼠子宫颈张力的影响及作用机理

去氢表雄酮硫酸酯为正常人体肾上腺所分泌,是合成雄激素和雌激素的前体。1970年日本钟仿制药厂正式生产该产品作为宫颈成熟剂,用于治疗宫颈成熟不全和产程延长等。Mochizuki给妊娠末期的妇女ⅳ去氢表雄酮硫酸酯钠盐(OHA-S)100 mg,共2次,发现DHA-S能促进子     

阿托品对兔胸主动脉平滑肌收缩和细胞增殖的影响

用兔胸主动脉条研究Atr,Ver对CaCl₂,Atr对KCI量—效反应的影响。观察到Atr和Ver能抑制2种激动剂所致兔主动脉条的收缩,量一效曲线右移,最大反应降低,其pD₂值分别为4.4和5.8。两药也能明显抑制NE依内Ca²⁺性收缩,Atr对NE依外Ca²⁺性收缩影响较小,说明Atr主要对细胞外Ca²⁺经PDC所致的收缩有抑制作用。在兔ASMC培养中,有Ca²⁺时,Atr抑制ASMC增殖,无Ca²⁺时,Atr 20.6～185.2 μmol/L表现刺激增殖,555.7～1666.7 μmol/L则抑制MSMC增殖,说明Atr对ASMC作用也与Ca(2+)有关。