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991.
目的:评价聚羟基丁酸酯(PHB)膜引导种植体周围骨组织再生的效果。方法:在狗下颌骨即刻植入种植体的颊侧形成骨缺损,覆盖PHB膜,与钛膜及空白对照比较,术后1、2、3个月分别取标本,采用大体观察、X线摄片、组织学、四环素荧光标记方法观察骨组织再生情况。结果:PHB膜组种植体周围骨缺损区较空白组有更多的新生骨充填,加速了骨再生过程,与钛膜组类似。结论:PHB有良好的生物相容性,可以用作骨组织引导再生膜 相似文献
992.
TGF—β1抑制肝癌细胞株BEL—7402的增殖 总被引:2,自引:0,他引:2
目的 研究转化生长因子-β1对体外培养肝癌细胞株BEL-7402增殖的影响。方法 用^3H-TdR掺入方法检测TGF-β1对肝癌细胞株BEL-7402增殖的抑制率;用逆转录整合酶链方法检测转化生长因子-β1及其Ⅱ型受体在BEL-7402中的基因表达。 相似文献
993.
目的:探讨涎腺肿瘤细胞的形态分化、组织发生及其异质性。方法:采用光镜、免疫组化技术对8例腺淋巴瘤、3例嗜酸性腺瘤、12例多形性腺瘤及2例上皮-肌上皮癌进行研究,同时观察了56例胚胎颌下腺组织作为对照。结果:多形性腺癌中导管样结构系肿瘤性闰管结构和肿瘤性分泌管结构;腺淋巴瘤和嗜酸性腺瘤中导管结构系肿瘤性分泌管结构;上皮-肌上皮癌中导管结构系肿瘤性闰管结构;涎腺肿瘤组织结构及抗原表达复杂多样,与人胚涎腺发生过程中结构及抗原表达有相似之处。结论:涎腺肿瘤的复杂性系致瘤因素作用于不同干细胞或同一干细胞不同分化阶段所致。 相似文献
994.
一氧化氮及其合酶在家兔粥样硬化动脉的改变及L-精氨酸的作用 总被引:13,自引:3,他引:10
为探讨一氧化氮及其合酶在高胆固醇饮食诱导的动脉粥样硬化的改变及L-精氨酸的抗动脉粥样硬化作用,在高胆固醇饮食诱导的动脉粥样硬化模型上,检测了血清总胆固醇,血浆精氨酸,非对称性二甲基精氨酸和氧化型低密度脂蛋白浓度,主动脉一氧化氮合酶活性,一氧化氮生成量及内膜斑块面积。 相似文献
995.
Combined adenine phosphoribosyltransferase and N-acetylgalactosamine-6-sulfate sulfatase deficiency.
L Wang X Ou I Sebesta K Vondrak J Krijt M Elleder H Poupetova J Ledvinova J Zeman H A Simmonds J A Tischfield A Sahota 《Molecular genetics and metabolism》1999,68(1):78-85
We describe a Czech patient with combined adenine phosphoribosyltransferase (APRT) deficiency (2,8-dihydroxyadenine urolithiasis) and N-acetylgalactosamine-6-sulfate sulfatase (GALNS) deficiency (mucopolysaccharidosis Type IVA, Morquio disease A). Adenine and its extremely insoluble derivative, 2,8-dihydroxyadenine, were identified in the urine, and APRT deficiency was confirmed in erythrocytes. There was excessive excretion of keratan sulfate in the urine, and GALNS deficiency was confirmed in leukocytes. GALNS and APRT are both located on chromosome 16q24.3, suggesting that the patient had a deletion involving both genes. PCR amplification of genomic DNA indicated that a novel junction was created by the fusion of sequences distal to GALNS exon 2 and proximal to APRT exon 3, and that the size of the deleted region was approximately 100 kb. The deletion breakpoints were localized within GALNS intron 2 and APRT intron 2. Several other genes, including the alpha subunit of cytochrome B (CYBA), which is deleted or mutated in the autosomal form of chronic granulomatous disease, are located in the 16q24.3 region, but PCR amplification showed that this gene was present in the proband. A patient with hemizygosity for GALNS deficiency and APRT deficiency has been reported from Japan recently. These findings indicate that: (i) APRT is located telomeric to GALNS; (ii) GALNS and APRT are transcribed in the same orientation (centromeric to telomeric); and (iii) combined APRT/GALNS deficiency may be more common than hitherto realized. 相似文献
996.
997.
遗传性脊髓小脑型共济失调的CAG三核苷酸突变检测 总被引:18,自引:5,他引:13
目的 评价SCA1、SCA2、SCA3/MjD、SCA6、SCA7和DRPLA的CAG三核苷酸异常扩增突变「(CAG)n」,在中国人遗传性脊髓小脑型共济失调(spinocerebellar ataxia,SCA)患者的分布频率。方法 经聚合酶链反应、变性聚丙烯酰按凝胶电泳和银染显带技术,检测分析了85个中国人常染色体显性遗传SCA家系(其中患者167例)和37例散发SCA患者的SCA1、SCA2、 相似文献
998.
β七叶皂甙钠与甘露醇降低急性颅脑外伤患者颅内压的疗效比较 总被引:9,自引:6,他引:3
目的:探讨β七叶皂甙钠与甘露醇对降低急性颅脑外伤患者颅内压(ICP)的疗效和利尿作用的比较。方法:50例急性颅脑外伤患者(观察组)伤后应用β七叶皂甙钠20mg加生理盐水50ml静注,每日总量60mg,连用7日。对照组20例应用20%甘露醇250ml静注,每日2~3次,连用7日。检测2组患者ICP变化及尿量、尿比重变化。结果:β七叶皂甙钠降ICP作用的起效时间、作用高峰时间和作用持续时间〔分别为(0.55±0.31)h、(2.30±0.50)h和(5.30±1.96)h〕与20%甘露醇的作用〔分别为(0.40±0.12)h、(0.86±0.54)h和(4.23±1.50)h〕比较,均有显著性差异(P<0.05,P<0.001和P<0.01)。利尿作用和血电解质、生化指标变化,2组均无显著性差异。结论:β七叶皂甙钠降低急性颅脑外伤患者ICP作用明显,作用持续时间长于甘露醇,对肾脏损害作用小。 相似文献
999.
A L Buchman W O'Brien C N Ou C Rognerud M Alvarez K Dennis C Ahn 《International journal of sports medicine》1999,20(5):315-321
Gastrointestinal bleeding and increased intestinal permeability have been observed in marathon runners. We sought to determine if L-arginine would be useful for prevention of these complications. Twenty-three runners were randomized to receive L-arginine (A) or glycine (placebo) (G), 10 grams 3 times daily for 14 days prior to the 1997 Houston-Methodist Marathon. Serum, stool hemoccults and lactulose:mannitol permeabilities were obtained at baseline, immediately after completion of the marathon and approximately 48 hours later. Runners rated their symptoms of nausea and vomiting, belching and indigestion, abdominal pain and bloating, diarrhea, and extremity pain on a 1-5 scale of increasing severity. The L:M was unchanged in either group during the three collections. Occult bleeding occurred in 8%/20% in A and G groups, respectively, p = NS) immediately post-marathon. No runners had occult bleeding 48 hours post-race. Gastrointestinal symptom scores were minimal to nonexistent. Extremity pain scores were similar for groups A and G (2.1+/-1.4 and 2.8+/-1.6, respectively, (p = NS). Fluid intake was similar between both groups (1875+/-1547 vs. 1506+/-970 ml, p = NS). Serum amylase was normal at baseline and remained virtually unchanged. Serum lipase was normal at baseline and immediately post-race in both groups, but increased at 48 hours post-race (82.2+/-34.3 to 121.5+/-53.3 mg/dl [A], p = 0.02 and 114.3+/-55.7 to 181.9+/-162.2 mg/dl [G], p = 0.09). CPK increased significantly and similarly in both groups immediately post-race, and even more dramatically 48 hours post-race (130.3+/-130.8 to 738.8+/-902.9, p = 0.007 to 1966.5+/-3.166.0 mg/dl [A] and 140.9+/-77.9 to 863.0+/-772.3, p = 0.003 to 5619+/-10636.8mg/dl [G]). Modest post-race decreases were seen in most serum amino acids in both groups. Finish times were longer than predicted (23+/-21 and 9+/-7 min for A and G groups, respectively, p = 0.049). Our study failed to show a clear benefit of arginine supplementation for the prevention of intestinal ischemia/reperfusion injury associated with endurance running, but either a detrimental affect on performance with arginine, or enhanced performance with glycine. Skeletal muscle injury was unaffected by arginine or glycine supplementation. The delayed increase in serum lipase suggests mild pancreatic injury, affected by either arginine or glycine supplementation. 相似文献
1000.