EFFECT OF POLICOSANOL SUCCESSIVE DOSE INCREASES ON PLATELET AGGREGATION IN HEALTHY VOLUNTEERS

Policosanol is a cholesterol-lowering drug with hypocholesterolemic effects demonstrated in experimental models, healthy volunteers and type II hypercholesterolemic patients. In addition, antiplatelet effects of policosanol have been shown in experimental models and healthy volunteers. The effect of successively increasing doses of policosanol on platelet aggregation was investigated in a randomized, placebo-controlled, double-blind study conducted in 37 healthy volunteers. The volunteers were on a placebo-baseline period (two tablets per day) for 7 days and thereafter they received randomly, under double-blind conditions, placebo or policosanol (10mgday⁻¹) for 7 days. After this period dosage was doubled to 20mgday⁻¹for the next 7 days and then again doubled to 40mgday⁻¹, while the control group received placebo tablets all the time. Platelet aggregation as well as coagulation time was measured at baseline and after each dosing step. Results showed that antiplatelet effects of policosanol were successfully enhanced throughout the study, thus suggesting a dose-dependent relationship. No significant effect was reached during the first dosing period, but significant reductions of epinephrine and ADP-induced platelet aggregation were observed after the second one. Finally, a significant inhibition of platelet aggregation induced by all the agonists was observed at the last dosing step. Coagulation time remained unchanged during the trial.     

下呼吸道感染菌的分布及耐药性分析

目的: 研究下呼吸道所感染病原菌的分布以及耐药状况,给临床治疗下呼吸道感染性疾病提供参考
依据,并且可以指导临床合理使用抗菌药物。方法: 对诊断为下呼吸道感染病人的深部痰液进行培养,共培养分
离出3936 株致病菌,对其鉴定及药物敏感试验的结果进行了回顾性分析。结果: 下呼吸道感染病原菌中鲍曼不
动杆菌检出率最高为905 株( 23%) ,其次为肺炎克雷伯菌818 株( 20．8%) 、铜绿假单胞菌768 株( 19．5%) 、金黄
色葡萄球菌405 株( 10．3%) 、大肠埃希菌350 株( 8．9%) ; 对亚胺培南的耐药率: 大肠埃希菌最低为0．1%,肺炎克
雷伯菌为0．3%。鲍曼不动杆菌和铜绿假单胞菌的耐药率较高,未发现耐万古霉素金黄色葡萄球菌。结论: 下呼
吸道感染主要病原菌为鲍曼不动杆菌和肺炎克雷伯菌,对头孢类和喹诺酮类药物的耐药率明显较高,临床应依
据药敏报告结果合理选用抗菌药物。     

农药多菌灵对大青叶有效成分靛蓝、靛玉红含量的影响

目的:探讨农药多菌灵对大青叶有效成分靛蓝、靛玉红含量的影响。方法:通过田间试验设计3个试验组,在9个不同时间段内采集样品,采用高效液相色谱法测定大青叶中靛蓝、靛玉红的含量。结果:施用高、低浓度多菌灵后大青叶的有效成分均低于空白对照组。结论:施用一定浓度的多菌灵可降低大青叶中有效成分的含量。     

High prevalence of high-risk oncogenic human papillomaviruses harboring atypical distribution in women of childbearing age living in Libreville, Gabon

The extent of human papillomavirus (HPV) genital shedding and type-specific diversity were evaluated in 354 consecutive women of childbearing age living in Libreville, Gabon. Detection of HPV DNA was performed by PCR using the MY09/MY11 primer set on DNA extracted from endocervical swabs. All PCR positive specimens were subjected to direct sequencing and HPV genotypes were identified on the basis of >95% sequence homology in the L1 region. Reverse line blot hybridization assay was used when a genotype could not be resolved by sequencing alone. HPV DNA was detected in 163 (46%) women, all clinically asymptomatic for HPV-related lesions. The highest prevalence of genital HPV detection (45%) was in the age group from 22 to 29 years. A total of 90 women (55%) harbored high-risk (HR) genotypes, with the most common being HPV-53 (19; 12%), HPV-58 (17; 11%), and HPV-16 (16; 10%). Low-risk genotypes were found in 36 (22%) women with HPV-54 and HPV-70 being the most frequently detected (17; 11% and 10; 6%, respectively). Finally 37 women (23%) tested positive for genotypes of unknown oncogenic risk, the most common in this category being HPV-83 (20; 12%). Multiple infections were detected in 35 (21%) women. By multivariate analysis, HPV genital shedding was significantly associated with young age (OR: 0.34; P < 0.007). The multivalent vaccine currently available against cervical carcinomas, is only active against HPV-16 and HPV-18, and will thus have a low impact in this setting.     

三门峡市湖滨区2007年人群死因统计及寿命分析

[目的]了解三门峡市湖滨区2007的人口死因情况,为疾病预防控制提供研究信息.[方法]死因编码采用ICD-10分类,用Excel 2003对全人群死因资料进行统计分析,用现时寿命表计算期望寿命.[结果]三门峡市湖滨区2007年总体死亡率为604.55/10万,男性死亡率648.41/10万,女性死亡率552.56/10万,死因居前3位的分别是循环系统疾病、恶性肿瘤和呼吸系统疾病,人群期望寿命为74.75岁,去肿瘤人群期望寿命为78.11岁.[结论]循环系统疾病、恶性肿瘤和呼吸系统疾病以及意外伤害死亡是三门峡市湖滨区2007年的主要死因.     

国内康复医学相关资源网站综合评估与分析

目的：探讨网络技术在康复医学资源共享领域的运用。方法：收集近五年来国内有代表性的康复医学资源网站或网络技术，按照各自属性分为学术组织类、专职机构类、特色康复医疗专业类、个体类四组，从一般普通网站共有的特征以及与康复医学资源共享相关的指标两方面进行了分析和比较。结果：所有网站在一般的总体性能指标上差异并不明显，但在与资源共享相关的指标评测中，各类网站总体差异很大。结论：网络技术在康复医学资源共享领域的应用正处于发展阶段，诸多方面尚须深人探究，但将有限的资源最大化共享对推动康复医学事业的整体发展将起到难以估量的作用。     

The inhibition of Src kinase suppresses the production of matrix metalloproteinases in from synovial fibroblasts and inhibits MAPK and STATs pathways

Background/aimThe purpose of this study was to investigate the antiarthritic potentials of the inhibition of Src kinase in vivo and in vitro settings. Materials and methodsArthritis was induced by intradermal injection of chicken type II collagen combined with incomplete Freund’s adjuvant (collagen induced arthritis [CIA] model) in Wistar albino rats. One day after the onset of arthritis, dasatinib, a potent Src kinase inhibitor, (5 mg/kg/day) was given via oral gavage. Tissue Src, Fyn, MAPK and STAT mRNA expressions were determined by real-time polymerase chain reaction. On the other hand, fibroblast like synoviocytes (FLSs) were harvested patients with rheumatoid arthritis (RA) undergoing surgical knee joint replacement. FLSs were stimulated with cytokines and dasatinib was added in different concentrations. MMP –1, –3, and –13 levels in FLSs culture were determined by ELISA.ResultsThe tissue mRNA expressions of Src, Fyn, MAPK and STATs were increased in the arthritis CIA group compared to the control group. Their mRNA expressions in the CIA + dasatinib group were decreased and similar in the control group. In in vitro setting, MMP –1, –3, and –13 expressions from FLSs induced by IL-1β and TNF-α were increased, while dasatinib suppressed their productions from FLSs.ConclusionThe present study shows that the inhibition of Src kinase has antiarthritic potentials in both in vivo and in vitro settings. Src kinase inhibition may be candidate to further research in human RA.     

徐光星教授依主次病机从“燥”论治肺癌经验

[目的] 总结徐光星教授依主次病机从燥热伤肺、气阴不足论治肺癌的经验。[方法] 通过跟诊学习，整理相关医案，查阅历代中医文献及国医大师何任教授辨治肿瘤的相关论述与治疗肺癌验案，从病因病机、辨治思路两方面，分析徐教授依主次病机，从燥热伤肺、气阴不足辨治肺癌的临床经验，并佐以两则医案验证。[结果] 徐教授认为，肺癌的形成是由于正气内虚、邪毒侵袭、燥热伤肺、痰瘀互结所致，提出以燥热伤肺、气阴不足为主次病机治疗肺癌，并传承何任教授从益气养阴治疗中晚期肺癌的基本大法，治疗或以燥热伤肺、气阴不足为主病机，清热润燥、益气养阴为主法；或以燥热伤肺、气阴不足为次病机，化痰散结、活血化瘀、清热解毒为主法，兼以清热润燥、益气养阴。所附医案一辨为肺癌后期，以燥热伤肺、气阴不足为主病机，治拟益气养阴、清热润燥、化痰散结；医案二辨为肺癌中期，以痰热壅肺为主病机，燥热伤肺、气阴不足为次病机，治拟清热化痰、养阴润燥，均药治稳好。[结论] 徐光星教授以燥热伤肺、气阴不足为主次病机论治肺癌，疗效确切，临床实用，值得学习与推广。