Improved gas chromatographic quantitation of breath hydrogen by normalization to respiratory carbon dioxide.

A gas-solid chromatographic system using tandem silica gel and molecular sieve columns is described for the measurement of hydrogen, carbon dioxide, oxygen, and nitrogen in samples of respiratory gases. This system has a detection limit of 2 ppm of hydrogen in a 1 ml sample and can measure 120 ppm H2 and 5% CO2 with relative standard deviations of 1.3% and 1.7%, respectively. Improved sample storage and withdrawal techniques are described that give reproducible values for up to 6 weeks after collection. Finally we show that normalization of breath hydrogen values to an alveolar concentration, using the observed carbon dioxide concentrations, substantially reduces the range and variance of apparent H2 concentrations in human subjects. Normalization eliminates the need for rebreathing or end-expiratory collection techniques and substantially increases the reliability and clinical utility of hydrogen breath measurements in noninvasive tests of carbohydrate malabsorption.     

A Clinical and Experimental Study on the Protective Effects of Jiangzhi Tongmai Fang on Endothelial Injury

31 cases of atherosclerosis (AS) were treated with Jiang Zhi Tong Mai Fang ([symbol: see text], formula of JZTMF), and its effect was compared with 30 cases treated with lovastatin in the control group. Clinically, the JZTMF formula showed an effect of regulating blood lipids, and therefore it was antiatherosclerotic. The mechanism is, probably, restoration of the function of endothelial cells (EC) by increasing the synthesis of 6-keto-PGF1 alpha and decreasing the release of endothelin (ET) as evidenced in the experimental study.     

SPONTANEOUS DISRUPTION OF BOVINE PERICARDIAL PROSTHESIS CLINICAL MANAGEMENT AND HISTOPATHOLOGIC CHANGES

Valve failure is rate after using glutaraldehyde stabilized bovine pericardial bioprosthesis in the mitral position.i This article reports a case of spon- taneous disruption of bovine pericardial xenograft after mitral valve replacement five years after opera- tion.     

CHRONIC LYMPHOCYTIC THYROIDITIS IN ENDEMIC GOITER

ChroLnic lymphocytic thyroiditis and lympho- cyte infiltration were studied in 2,053 cases of endemic goiter. Only 7 cases of chronic lympho- cytic thyroiditis and 3 0f focal thyroiditis showed diffused enlargement of thyroid. Among 2,053 cases, 150 had thyroiditis (7.3%), of whom 137 (7.1%) were accompanied by nodular goiters. If focal thyroiditis was excludetl, the incidence rate of chronic lymphocytic thyroiditis would be 2.39T% The increased incidence of Hashimoto's disease was considered to he due to the decreased incidence of endemic goiter and the neglect of chronic lymphocytic thyroiditis accompanied by nodular goiters. Thyroiditis occurs in the sur- rounding tissues of the nodule, and it is why the changes in thyroiditis were overlooked iii excised nodules.     

RAPID ASSAY OF INSUU[N IN PORTOSPLENIC BLOOD FOR INSULINOMA

Intraoperative rapid insulin assay has been successfully used for a year in our hospital for monitoring completeness of insulinoma excision during operations on 5 patients* According to our experience, portal blood insulin levels fell sharply shortly after removal of insulinoma. Wicth rapid immunoreactive insulin assay9 the time between sample taking and final reading was about 45 minutes. As blood sugar monitoring is influenced by many factors and correct result can only be obtained in an average of 70 minutes or even longer after complete remoival of tumor, rapid immunoreactive insulin assay has evident advantages over blood sugar monitoring.     

氧化型低密度脂蛋白诱导主动脉和心内膜内皮细胞凋亡

目的：研究氧化型低密度脂蛋白（ｏｘ－ＬＤＬ）诱导血管和心内膜内皮细胞凋亡。方法：用超速离心法分离健康人血浆低密度脂蛋白（ＬＤＬ），以ＣｕＳＯ４１０μｍｏｌ．Ｌ＾－１氧化，观察ｏｘ－ＬＤＬ对培养新生小牛主动脉内皮细胞及心内膜细胞的损伤作用，琼脂糖凝胶电泳和Ｈｏｅｃｈｓｔ３３２５８荧光密度法定性与定量分析ＤＮＡ降解，结果：ｏｘ－ＬＤＬ诱导血管内皮细胞及以内膜细胞典型凋亡形态学改变，ＤＮＡ降解呈时间和剂     

Laser photobiostimulation-induced hypoalgesia in rats is not naloxone reversible

Laser photobiostimulation (LPBS) at the pulsing frequency of 4 Hz applied to the low resistance point located at the base of the tail of the rat, (Governing Vessel Meridian 1), produced a hypoalgesic effect, measured by tail-flick and hot-plate techniques. Pre-treatment with low dose naloxone (2 mg/kg) did not reverse the hypoalgesic effect of LPBS. High dose naloxone (20 mg/kg) reversed only partially, but significantly, the hypoalgesic effect of LPBS measured by hot-plate, but not that measured by the tail-flick technique. These data suggest that mechanisms other than endogenous opioids may be involved in LPBS-induced hypoalgesia.     

急进高原前后战士无氧阈的调查研究

报道60名新兵空运进驻3680m 前后通气无氧阈(ATGE)测定结果。受试者进入高原初期,负荷运动达无氧阈时,负荷功率和摄氧量均降低,心率升高;达最大负荷运动时,负荷功率,摄氧量和心率均降低,通气量升高。进驻高原前后各参数均存在显著性差异(P<0.01)。研究认为,ATGE 是很有价值而可靠的心肺功能评价指标,尤以 ATGE(负荷功率)最为敏感,其次为 ATGE(Vo2)。     

腹横纹切口保留睾丸引带阴囊皮下睾丸固定术治疗隐睾症的研究

为了研究治疗先天性隐睾症的最佳术式，以提高隐睾症的治疗水平，采用腹横纹切口保留睾丸引带明囊皮下睾丸固定术（研究组）治疗隐睾症96例110枚，获得随访者94例108枚，同时采用患倒下腹斜切口肉膜囊睾丸固定术（对照组）治疗隐睾症50例62枚。结果研究组睾丸大小及硬度化94枚，占87．0％，良9枚，占8．3％，差5枚，占4．6％；睾丸位置优93枚，占86．1％，良9枚，占8．3％，差6枚，占5．6％；无睾丸萎缩及回缩，外表美观。对照组睾丸大小及硬度优41枚，占66．1％，良7枚，占11．3％，差14枚，占22．6％；睾丸位置优43枚，占69．4％，良8枚，占12．9％，差11枚，占17．7％。经统计学处理，两组睾丸大小及硬度方面比较有极显著性差异（P＜0．01），睾丸位置比较也有显著性差异（P＜0．05）。认为腹横纹切口保留睾丸引带阴囊皮下睾丸固定术损伤小，外表美观，明显降低了睾丸萎缩及回缩等并发症，符合生理要求，疗效满意。     

用生物素标记CVB_3 cDNA制备探针检测病毒核酸

本文报道用含CVB_3 cDNA PstI酶切片段(530bp)的重组质粒pGP_(51)B转化大肠菌;经扩增后提取,纯化重组质粒DNA;用生物素标记制备探针。通过原位杂交技术检测不同的肠道病毒和非肠道病毒感染的HeLa细胞。实验结果表明,该探针只与肠道病毒RNA杂交,而不与非肠道病毒核酸杂交,且可检测出病毒感染后3h,5h(CPE-)的病毒核酸。可见该探针具有良好的特异性和敏感性,且可用于肠道病毒感染引起的心脏疾病的活检标本诊断。