粪菌移植在儿科应用的研究进展北大核心CSCD

粪菌移植(FMT)可将健康人粪便中的各种肠道微生物、代谢产物和天然抗菌物质等移植到受者肠道内,可重建肠道菌群平衡、修复肠黏膜屏障、控制炎症反应、调节机体免疫,是治疗肠道菌群失调所致疾病的新方法。FMT治疗儿童复发性艰难梭菌感染已写入复发性艰难梭菌感染的诊疗指南。FMT治疗儿童炎症性肠病、孤独症谱系障碍的有效性及安全性较好。     

双甲五灵胶囊与肝纤维化血清TIMP-1的变化及临床疗效观察

目的观察自行研制的中药双甲五灵胶囊治疗慢性肝炎患者前后血清基质金属蛋白酶组织抑制剂1(TIMP1)的变化及意义,并观察双甲五灵胶囊治疗的临床疗效。方法将226例慢性肝炎患者随机分为双甲五灵胶囊治疗组和对照组,观察两组治疗半年前后临床症状、体征、肝功能、血清透明质酸(HA)、Ⅲ型前胶原(PCⅢ)、Ⅳ型胶原(CⅣ)、层粘连蛋白(LN)以及TIMP1的变化,并进行统计学处理。结果双甲五灵胶囊治疗组与对照组比较,治疗组患者临床症状、体征、肝功能及血清肝纤维化指标明显好转(P<0.01)。结论双甲五灵胶囊可使患者血清中TIMP1水平明显降低,用于防治肝纤维化有效。双甲五灵胶囊对防治慢性肝炎肝纤维化有一定效果。     

HBV感染者血清中HBVcccDNA、HBeAg及HBV DNA的关系

探讨HBV感染者血清HBVcccDNA与血清HBV DNA及HBeAg的关系。分别以PER分子信标技术和ELISA方法对非HBV相关肝炎、HBV健康携带者、急性乙型肝炎（AHB）、慢性乙型肝炎（CHB）、乙肝肝硬化、乙肝患者血清中HBVcccDNA HBV DNA含量及HBeAg进行了检测。HBVcccDNA仅见于HBV DNA阳性血清中；HBeAg阳性组的HBVcccDNA阳性率显著高于HBeAg阴性组（P〈0．05）；145例HBV DNA阳性患者中，HBVcccDNA阳性组HBVD—NA水平显著高于HBVcccDNA阴性组（P〈0．01）。血清HBVcccDNA可能是乙肝病毒在患者体内大量复制的血清标志。     

蚤莲合剂对DEN所致大鼠肝癌前病变的影响

目的:观察蚤莲合剂对诱发大鼠原发性肝癌的抗促癌作用及其机理。方法:以含100ppm的二乙基亚硝胺(DEN)饮水造模,采用蚤莲合剂灌胃给药,观察其对大鼠肝组织病理学、免疫组化、血清和肝组织生物化学指标的影响。结果:蚤莲合剂对大鼠肝组织癌前病变有抑制作用。大鼠肝癌形成率相对减少,血清ALT、ALb、ALP、TNF水平及肝匀浆γ-GT活性明显低于模型组(P<0.01),其免疫组化AFP阳性细胞数也较模型组为少(P<0.05)。结论:蚤莲合剂有明显抑制DEN所致大鼠肝癌前病变的作用,其抑癌作用可能与其护肝、抗炎和控制变异细胞过度合成有关。     

Development of chitosan/gelatin hydrogels incorporation of biphasic calcium phosphate nanoparticles for bone tissue engineering

Abstract

The chitosan/gelatin hydrogel incorporated with biphasic calcium phosphate nanoparticles (BCP-NPs) as scaffold (CGB) for bone tissue engineering was reported in this article. Such nanocomposite hydrogels were fabricated by using cycled freeze-thawing method, of which physicochemical and biological properties were regulated by adjusting the weight ratio of chitosan/gelatin/BCP-NPs. The needle-like BCP-NPs were dispersed into composites uniformly, and physically cross-linked with chitosan and gelatin, which were identified via Scanning Electron Microscope (SEM) images and Fourier Transform Infrared Spectroscopy (FT-IR) analysis. The porosity, equilibrium swelling ratio, and compressive strength of CGB scaffolds were mainly influenced by the BCP-NPs concentration. In vitro degradation analysis in simulated body fluids (SBF) displayed that CGB scaffolds were degraded up to at least 30?wt% in one month. Also, CCK-8 analysis confirmed that the prepared scaffolds had a good cytocompatibility through in culturing with bone marrow mesenchymal stem cells (BMSCs). Finally, In vivo animal experiments revealed that new bone tissue was observed inside the scaffolds, and gradually increased with increasing months, when implanted CGB scaffolds into large necrotic lesions of rabbit femoral head. The above results suggested that prepared CGB nanocomposites had the potential to be applied in bone tissue engineering.     

股深动脉第1穿动脉穿支皮瓣修复臀股部IV期压疮疗效

目的　探讨应用股深动脉第1穿动脉穿支皮瓣修复臀股部IV期压疮的可行性。 方法　2016年1月至2019年2月,本院手足创伤骨科收治9例臀股部IV期压疮患者,男3例,女6例,年龄26~68岁,平均46岁。截瘫原因：高处坠落伤4例,车祸伤3例,脑梗塞2例。清创后创面面积8 cm×5 cm~18 cm×9 cm。根据创面形状、大小及位置设计股深动脉第1穿动脉穿支皮瓣修复压疮创面,皮瓣面积为10 cm×7 cm~20 cm×10 cm。 结果　所有皮瓣成活,其中2例术后局部渗液较多,经积极保守换药治疗后,皮瓣恢复良好。9例获得6~24月随访,平均13月,皮瓣色泽良好,质地柔软,不影响日常生活,压疮未见复发。 结论　股深动脉第1穿动脉穿支皮瓣操作简便,血供可靠,可供组织量较多,是修复臀股部IV期压疮的较好选择。