全文获取类型
收费全文 | 3050篇 |
免费 | 175篇 |
国内免费 | 10篇 |
专业分类
耳鼻咽喉 | 38篇 |
儿科学 | 86篇 |
妇产科学 | 74篇 |
基础医学 | 428篇 |
口腔科学 | 337篇 |
临床医学 | 298篇 |
内科学 | 487篇 |
皮肤病学 | 46篇 |
神经病学 | 224篇 |
特种医学 | 83篇 |
外科学 | 370篇 |
综合类 | 8篇 |
一般理论 | 2篇 |
预防医学 | 365篇 |
眼科学 | 38篇 |
药学 | 203篇 |
中国医学 | 29篇 |
肿瘤学 | 119篇 |
出版年
2023年 | 25篇 |
2022年 | 42篇 |
2021年 | 97篇 |
2020年 | 80篇 |
2019年 | 96篇 |
2018年 | 125篇 |
2017年 | 77篇 |
2016年 | 82篇 |
2015年 | 106篇 |
2014年 | 168篇 |
2013年 | 154篇 |
2012年 | 287篇 |
2011年 | 252篇 |
2010年 | 160篇 |
2009年 | 111篇 |
2008年 | 215篇 |
2007年 | 199篇 |
2006年 | 146篇 |
2005年 | 160篇 |
2004年 | 134篇 |
2003年 | 110篇 |
2002年 | 96篇 |
2001年 | 39篇 |
2000年 | 30篇 |
1999年 | 32篇 |
1998年 | 15篇 |
1997年 | 11篇 |
1996年 | 17篇 |
1995年 | 9篇 |
1994年 | 12篇 |
1993年 | 8篇 |
1992年 | 13篇 |
1991年 | 12篇 |
1990年 | 4篇 |
1989年 | 6篇 |
1988年 | 9篇 |
1987年 | 7篇 |
1986年 | 3篇 |
1985年 | 6篇 |
1984年 | 9篇 |
1983年 | 7篇 |
1982年 | 6篇 |
1981年 | 8篇 |
1980年 | 10篇 |
1976年 | 3篇 |
1971年 | 3篇 |
1970年 | 4篇 |
1969年 | 4篇 |
1963年 | 2篇 |
1962年 | 3篇 |
排序方式: 共有3235条查询结果,搜索用时 15 毫秒
91.
92.
93.
Cuervo P de Jesus JB Junqueira M Mendonça-Lima L González LJ Betancourt L Grimaldi G Domont GB Fernandes O Cupolillo E 《Molecular and biochemical parasitology》2007,154(1):6-21
Leishmania (Viannia) braziliensis, a protozoan parasite widespread in the New World, is responsible for the infection of different mammal orders, including humans. This species is considered to be a major etiological agent of American cutaneous leishmaniasis. A proteomic study was carried out to identify proteins expressed by L. (V.) braziliensis. One hundred and one spots representing 75 protein entries were identified by MALDI-TOF-TOF. Isoelectric point values estimated by gel electrophoresis matched closely with predicted values, although some discrepancies existed suggesting that post-translational protein modifications may be common in L. braziliensis. Moreover, 20 hypothetical proteins were experimentally identified. Identified proteins were classified into 15 groups according to biological process. Among the proteins identified, approximately 40% have not been previously reported in a proteomic map of Leishmania. In addition, a number of potential virulence factors and drug targets were identified in this protein map, including some proteins associated with the metastatic phenotype. This study describes the first compilation of a proteomic reference map for L. braziliensis (pI 4-7, M(r) 10-130 kDa) and provides a very useful tool for comparative studies of strains isolated from patients presenting different clinical manifestations of leishmaniasis as well as a potential tool to identify markers for clinical diagnosis, therapeutics, and prognosis. 相似文献
94.
da Silveira EB Cordeiro BA Ribeiro BM de Castro ME Soares EF Báo SN 《Virus research》2007,130(1-2):182-192
An Anticarsia gemmatalis multiple nucleopolyhedrovirus (AgMNPV) mutant, vApAg, induces apoptosis in a cell culture derived from Anticarsia gemmatalis (UFL-AG-286), reducing viral progeny. We have investigated apoptosis induction in vivo by vApAg in A. gemmatalis larvae and its correlation to infectivity reduction. LC50, LD50, LT50 and the mean time to death of larvae were determined for vApAg and AgMNPV. Apoptosis was accessed for hemocytes of infected larvae using light and transmission electron microscopy. All types of hemocytes can be infected by vApAg. After 12 h post-infection (h p.i.), typical cellular modifications associated to nucleopolyhedrovirus infection were observed. Apoptosis becomes evident after 24 h p.i., and massive after 72 h p.i. Necrosis of infected cells was also observed. Despite cell death, hemocytes produced budded viruses and polyhedra. Pl and gh1-type hemocytes presented phagocytic activity. Agarose gel electrophoresis revealed fragmentation of hemocytes DNA at late times post-infection. The LC50 and LD50 were between five- and six-fold higher for vApAg. The LT50 and the mean time to death were higher for vApAg in a same treatment or for a similar mortality induced by AgMNPV. These results show correlation of apoptosis and the reduced infectivity of vApAg in A. gemmatalis larvae. 相似文献
95.
OBJECTIVE: Increasingly clinicians other than genetic counselors will advise people with genetic risks. Although some express concerns about this development because of the need for non-genetic clinicians to have additional training, we argue that genetic counseling has more in common with other health care interactions than is generally assumed. METHODS: In this narrative review we investigate the health communication literature taking the perspective that all provider-patient/client interactions share the following goals: forming a relationship, the exchange of information, decision making, promoting health-related behavior and providing support. RESULTS: We found that both non-genetic and genetic 'disciplines' endorse an egalitarian relationship, based on a patient-centered approach and both have difficulties with attuning to the patients' agendas and enhancing patient understanding. Shared decision making is increasingly the preferred model for geneticists and non-geneticists alike, and both need skills to constructively discuss patients' risk-reducing behavior and provide emotional support. CONCLUSION: Rather than developing separate vocabularies and research traditions, the discipline of genetic counseling may benefit by drawing on non-genetic patient-provider interaction research. PRACTICE IMPLICATIONS: Since geneticists face the same challenges as non-geneticists, medical training should continue to improve basic consultation skills, regardless of whether the consultation involves genetic information. 相似文献
96.
Fas ligand-dependent inflammatory regulation in acute myocarditis induced by Trypanosoma cruzi infection 总被引:1,自引:0,他引:1
下载免费PDF全文
![点击此处可从《The American journal of pathology》网站下载免费的PDF全文](/ch/ext_images/free.gif)
de Oliveira GM Diniz RL Batista W Batista MM Bani Correa C de Araújo-Jorge TC Henriques-Pons A 《The American journal of pathology》2007,171(1):79-86
Fas/Fas ligand (Fas-L) engagement, a potent inducer of apoptosis, is also important for cellular activation, regulation of effector and chemotactic activity, and secretion of chemokines and cytokines. We evaluated the relevance of Fas/Fas-L in the regulation of myocarditis induced by Trypanosoma cruzi infection and observed that in Fas-L(-/-) mice (gld/gld), cardiac infiltration was significantly reduced, accordingly showing less cardiomyocyte destruction. Fluorescence-activated cell sorting analysis of cardiac inflammatory cells showed higher numbers of CD8(+) T cells in BALB/c compared with gld/gld mice but similar levels of lymphocyte function-associated antigen-1, intercellular adhesion molecule, CD2, and CD69 expression; MAC-1(+) myeloid cells and mast cells were increased in BALB/c mice, whereas gld/gld mice exhibited an enrichment of CD4(+/low) T cells. Intracellular labeling of cytokines revealed no clear cardiac skewing of Th1 or Th2 responses, but we found a higher number of interleukin-10(+) cells in gld/gld mice and a deficient expression of vascular cell adhesion molecule-1 on cardiac endothelial cells in gld/gld mice. Finally, we found a population of CD3(+) but CD4/CD8 double negative cardiac T cells in both groups of infected mice, but down-regulation of some adhesion molecules and surface receptors was only observed in gld/gld mice, indicating a targeted T-cell population mostly affected by the lack of Fas-L engagement. These results point to a role for myocarditis regulation by Fas/Fas-L beyond its possible direct relevance in cellular death. 相似文献
97.
Batista AP Santhanam G Yu BM Ryu SI Afshar A Shenoy KV 《Journal of neurophysiology》2007,98(2):966-983
When a human or animal reaches out to grasp an object, the brain rapidly computes a pattern of muscular contractions that can acquire the target. This computation involves a reference frame transformation because the target's position is initially available only in a visual reference frame, yet the required control signal is a set of commands to the musculature. One of the core brain areas involved in visually guided reaching is the dorsal aspect of the premotor cortex (PMd). Using chronically implanted electrode arrays in two Rhesus monkeys, we studied the contributions of PMd to the reference frame transformation for reaching. PMd neurons are influenced by the locations of reach targets relative to both the arm and the eyes. Some neurons encode reach goals using limb-centered reference frames, whereas others employ eye-centered reference fames. Some cells encode reach goals in a reference frame best described by the combined position of the eyes and hand. In addition to neurons like these where a reference frame could be identified, PMd also contains cells that are influenced by both the eye- and limb-centered locations of reach goals but for which a distinct reference frame could not be determined. We propose two interpretations for these neurons. First, they may encode reach goals using a reference frame we did not investigate, such as intrinsic reference frames. Second, they may not be adequately characterized by any reference frame. 相似文献
98.
99.
A framework for testing the equality between the health concentration curve and the 45‐degree line
下载免费PDF全文
![点击此处可从《Health economics》网站下载免费的PDF全文](/ch/ext_images/free.gif)
The health concentration curve is the standard graphical tool to depict socioeconomic health inequality in the literature on health inequality. This paper shows that testing for the absence of socioeconomic health inequality is equivalent to testing if the conditional expectation of health on income is a constant function that is equal to average health status. In consequence, any test for parametric specification of a regression function can be used to test for the absence of socioeconomic health inequality (subject to regularity conditions). Furthermore, this paper illustrates how to test for this equality using a test for parametric regression functional form and applies it to health‐related behaviors from the National Health Survey 2014. 相似文献
100.