全文获取类型
收费全文 | 6969篇 |
免费 | 485篇 |
国内免费 | 23篇 |
专业分类
耳鼻咽喉 | 55篇 |
儿科学 | 283篇 |
妇产科学 | 162篇 |
基础医学 | 1109篇 |
口腔科学 | 64篇 |
临床医学 | 726篇 |
内科学 | 1339篇 |
皮肤病学 | 96篇 |
神经病学 | 781篇 |
特种医学 | 169篇 |
外科学 | 838篇 |
综合类 | 43篇 |
预防医学 | 686篇 |
眼科学 | 66篇 |
药学 | 572篇 |
中国医学 | 32篇 |
肿瘤学 | 456篇 |
出版年
2023年 | 39篇 |
2022年 | 75篇 |
2021年 | 185篇 |
2020年 | 106篇 |
2019年 | 166篇 |
2018年 | 188篇 |
2017年 | 134篇 |
2016年 | 156篇 |
2015年 | 185篇 |
2014年 | 227篇 |
2013年 | 330篇 |
2012年 | 469篇 |
2011年 | 532篇 |
2010年 | 279篇 |
2009年 | 262篇 |
2008年 | 446篇 |
2007年 | 462篇 |
2006年 | 434篇 |
2005年 | 410篇 |
2004年 | 422篇 |
2003年 | 377篇 |
2002年 | 357篇 |
2001年 | 81篇 |
2000年 | 66篇 |
1999年 | 84篇 |
1998年 | 79篇 |
1997年 | 64篇 |
1996年 | 63篇 |
1995年 | 53篇 |
1994年 | 43篇 |
1993年 | 37篇 |
1992年 | 52篇 |
1991年 | 44篇 |
1990年 | 44篇 |
1989年 | 40篇 |
1988年 | 44篇 |
1987年 | 38篇 |
1986年 | 31篇 |
1985年 | 29篇 |
1984年 | 40篇 |
1983年 | 30篇 |
1982年 | 27篇 |
1981年 | 25篇 |
1980年 | 33篇 |
1979年 | 38篇 |
1978年 | 19篇 |
1977年 | 14篇 |
1974年 | 16篇 |
1973年 | 16篇 |
1972年 | 18篇 |
排序方式: 共有7477条查询结果,搜索用时 15 毫秒
61.
Mégarbané A Gannagé-Yared MH Khalifé AA Fabre M 《American journal of medical genetics. Part A》2003,(2):214-217
Two sisters with primary hypergonadotropic hypogonadism associated with microcephaly, flat occiput, partial alopecia, absent or streak ovaries, and Müllerian hypoplasia are reported. Their parents are first cousins. Despite some clinical differences, their features were very close to a family described with such an association by Al-Awadi et al. [1985: Am J Med Genet 22:619-622] in Kuwait. 相似文献
62.
Honey K Benlagha K Beers C Forbush K Teyton L Kleijmeer MJ Rudensky AY Bendelac A 《Nature immunology》2002,3(11):1069-1074
CD1d antigen presentation to natural killer T (NKT) cells expressing the semi-invariant T cell receptor V(alpha)14J(alpha)18 requires CD1d trafficking through endosomal compartments; however, the endosomal events remain undefined. We show that mice lacking the endosomal protease cathepsin L (catL) have greatly reduced numbers of V(alpha)14(+)NK1.1(+) T cells. In addition, catL expression in thymocytes is critical not only for selection of these cells in vivo but also for stimulation of V(alpha)14(+)NK1.1(+) T cells in vitro. CD1d cell-surface expression and intracellular localization appear normal in catL-deficient thymocytes, as does the lysosomal morphology; this implies a specific role for catL in regulating presentation of natural CD1d ligands mediating V(alpha)14(+)NK1.1(+) T cell selection. These data implicate lysosomal proteases as key regulators of not only classical major histocompatibility complex class II antigen presentation but also nonclassical CD1d presentation. 相似文献
63.
Taylor GP Bodéus M Courtois F Pauli G Del Mistro A Machuca A Padua E Andersson S Goubau P Chieco-Bianchi L Soriano V Coste J Ades AE Weber JN 《Journal of acquired immune deficiency syndromes (1999)》2005,38(1):104-109
BACKGROUND: Up to 20 million persons are infected with the human retroviruses human T-lymphotropic virus (HTLV)-I and HTLV-II globally. Most data on the seroprevalence of HTLV-I and HTLV-II in Europe are from studies of low-risk blood donors or high-risk injection drug users (IDUs). Little is known about the general population. METHODS: A prospective anonymous study of HTLV-I and HTLV-II seroprevalence among 234,078 pregnant women in Belgium, France, Germany, Italy, Portugal, Spain, and the United Kingdom was conducted. Maternal antibody status was determined by standard methods using sera obtained for routine antenatal infection screens or eluted from infant heel prick dried blood spots obtained for routine neonatal metabolic screens. RESULTS: Anti-HTLV-I/II antibodies were detected and confirmed in 96 pregnant women (4.4 per 10,000, 95% confidence interval [CI]: 3.5-5.2). Of these, 73 were anti-HTLV-I, 17 were anti-HTLV-II, and 6 were specifically anti-HTLV but untyped. The seroprevalence ranged from 0.7 per 10,000 in Germany to 11.5 per 10,000 in France. CONCLUSIONS: Pregnant women better reflect the general population than blood donors or IDUs. The seroprevalence of HTLV-I and HTLV-II in Western Europe is 6-fold higher among pregnant women (4.4 per 10,000) than among blood donors (0.07 per 10,000). These data provide a robust baseline against which changes in HTLV-I and HTLV-II seroprevalence in Europe can be measured. 相似文献
64.
Audran M Krafft MP De Ceaurriz J Mathurin JC Sicart MT Marion B Fabre F Bressolle F 《Journal of chromatography. B, Biomedical sciences and applications》1999,734(2):267-276
This paper describes a GC-MS method (SIM mode) for the analysis of perfluorooctyl bromide (perflubron, I) in rat blood. The chromatographic separation was performed by injection in the split mode using a CP-select 624 CB capillary column. Following destruction of the emulsion by addition of ethanol, the analytical procedure involves a liquid-liquid extraction with 1,1,2-trichlorotrifluoroethane. The bis(F-butyl)ethene (II) was used as internal standard. Observed retention times were 3.22 min for I and 2.32 min for II. Two calibration curves were used; linear detection responses were obtained for concentrations ranging from 0.009 to 0.9 mg/ml and from 0.9 to 13.5 mg/ml. The extraction efficiency averaged 50% for I and 93% for II. Precision ranged from 0.7 to 14%, and accuracy was between 91 and 109%. The limit of quantification was 9 microg/ml. The method validation results indicate that the performance characteristics of the method fulfilled the requirements for assay method for use in pharmacokinetic studies. 相似文献
65.
Jeune syndrome and liver disease: report of three cases treated with ursodeoxycholic acid 总被引:1,自引:0,他引:1
Labrune P Fabre M Trioche P Estournet-Mathiaud B Grangeponte MC Rambaud C Maurage C Bernard O 《American journal of medical genetics》1999,88(4):324-328
Evidence for a bipolar disorder (BPD) susceptibility locus on chromosome 22q11 has been provided in several studies. One candidate gene that maps to this region is the G-protein alpha subunit gene Galphaz (GNAZ). We have identified a common silent polymorphism in GNAZ exon 2 by single strand conformation polymorphism analysis. The frequency of this polymorphism was determined in a control population (n=84) and in patients with BPD (n=88). The data showed a statistical trend toward a difference in the distribution of alleles in patients with BPD compared with control subjects (chi square=3.2, 1 df, P=0.073, two-tailed). No significant difference was detected when the GNAZ polymorphism was analyzed in control subjects and schizophrenia patients (n=63, P=0.92). These data continue to provide some support for a BPD susceptibility gene on 22q11, possibly in linkage disequilibrium with the GNAZ 309 polymorphism. 相似文献
66.
Study of the protective effects of hyperimmune immunoglobulins G and M against endotoxin in mice and rats 总被引:1,自引:0,他引:1
We prepared solutions of human IgM and IgG to various lipopolysaccharide (LPS) species. These were then tested, along with
solutions of non-LPS specific human IgG or IgM, for their ability to confer passive immunity against experimental endotoxemia
in two animal models. The immunoglobulins were first tested for an effect on the lethality induced by seven different LPSs
in actinomycin-D sensitized mice, or by three different bacteria in normal mice. When the immunoglobulins were administered
1 h before challenge, a small protective effect was observed. This protection was dependent upon both the anti-LPS agent,
the chemical composition of the LPS, or the strain of Gram-negative bacteria used for injection. The anti-LPS IgM and IgG
preparations reduced the mortality induced by Escherichia coli but not by Serratia marcescens or Klebsiella pneumoniae, indicating protection by strain-specific antibodies. When the antibodies were preincubated with LPS or bacteria for 30 min
before administration, almost complete protection was seen. The influence of these immunoglobulin preparations or of human
albumin (as a control) on the hypotensive and vascular-permeabilizing effects of LPS in rats was then studied. A dose-dependent
inhibitory effect was observed with IgG preparations and albumin. At 200 mg/kg, anti-LPS IgG reduced the effects of LPS, while
at 400 mg/kg, both anti-LPS and normal IgG preparations showed protection, as did human albumin used at the same dose. The
IgM-enriched preparation worsened the initial hypotensive phase after LPS, whereas the anti-LPS IgM significantly reduced
the second phase of the hypotension, but only at the largest dose of 400 mg/kg. In this second model using the rat, a clear
difference between the activity of IgG and IgM was thus observed. We conclude that pretreatment with human immunoglobulins
from large plasma pools modestly, but significantly, attenuated the effects of murine and rat Gram-negative sepsis, but that
protection was incomplete. Our results suggest that single regimen intervention strategies may not be sufficient to influence
the course of the disease.
Received: 12 December 1998 相似文献
67.
Daniel L Bohl Daniel C Brennan Caroline Ryschkewitsch Monique Gaudreault-Keener Eugene O Major Gregory A Storch 《Journal of clinical virology》2008,43(2):184-189
BACKGROUND: The mean urine BK viral load in kidney transplant recipients increases with the intensity of infection as the infection progresses from transient viruria to sustained viremia. OBJECTIVES: This study investigated whether the intensity of infection is associated with the humoral immune response. STUDY DESIGN: We measured BKV-specific IgG antibody titers in stored samples obtained serially over a 1-year period from 70 kidney transplant recipients with BKV infection and 17 control recipients without active BKV infection. RESULTS: The mean pre-transplant BKV antibody level was lower in recipients who developed viremia than the mean level in those who never developed viremia (p=0.004). Mean antibody titers in recipients who never showed evidence of active BKV infection rose slightly after transplant despite immunosuppression. The magnitude of the rise in the mean antibody titers in recipients who developed active BKV infection correlated with the intensity of infection (p<0.001). CONCLUSIONS: The mean antibody level increased in accordance with the intensity of the infection post-transplant. Pre-transplant seropositivity did not protect against sustained viremia and the antibody response was not associated with clearance of the virus. 相似文献
68.
For the diagnosis of imported malaria, a competitive multiplex PCR using LightCycler was developed and compared for 3 months with a traditional PCR method. Hundred eighty three patients were tested by these 2 techniques of molecular diagnosis: 60 were positive for P. falciparum, 9 were positive for other Plasmodium species and 114 were negative. The LightCycler method was found in total agreement with the traditional PCR. 相似文献
69.
High-intensity intermittent running training improves pulmonary function and alters exercise breathing pattern in children 总被引:2,自引:0,他引:2
Nourry C Deruelle F Guinhouya C Baquet G Fabre C Bart F Berthoin S Mucci P 《European journal of applied physiology》2005,94(4):415-423
We investigated the effects of short duration running training on resting and exercise lung function in healthy prepubescent children. One trained group (TrG) (n = 9; three girls and six boys; age = 9.7 ± 0.9 year) participated in 8 weeks of high-intensity intermittent running training and was compared to a control group (ContG) (n = 9; four girls and five boys; age = 10.3 ± 0.7 year). Before and after the 8-week period, the children performed pulmonary function tests and an incremental exercise test on a cycle ergometer. After the 8-week period, no change was found in pulmonary function in ContG. Conversely, an increase in forced vital capacity (FVC) (+7 ± 4% ; P = 0.026), forced expiratory volume in one second (+11 ± 6% ; P = 0.025), peak expiratory flows (+17 ± 4% ; P = 0.005), maximal expiratory flows at 50% (+16 ± 10% ; P = 0.019) and 75% (+15 ± 8% ; P = 0.006) of FVC were reported in TrG. At peak exercise, TrG displayed higher values of peak oxygen consumption (+15 ± 4% ; P<0.001), minute ventilation (+16 ± 5% ; P = 0.033) and tidal volume (+15 ± 5% ; P = 0.019) after training. At sub-maximal exercise, ventilatory response to exercise
was lower (P = 0.017) in TrG after training, associated with reduced end-tidal partial oxygen pressure (P<0.05) and higher end-tidal partial carbon dioxide pressure (P = 0.026). Lower deadspace volume relative to tidal volume was found at each stage of exercise in TrG after training (P<0.05). Eight weeks of high-intensity intermittent running training enhanced resting pulmonary function and led to deeper exercise ventilation reflecting a better effectiveness in prepubescent children. 相似文献
70.
Congenital stationary night blindness (CSNB) is a group of rare, non-progressive conditions of the retina characterized by abnormal rod function causing impaired night vision. Among them, the Schubert-Bornschein subgroup, itself divided into a complete and an incomplete form, is characterized by a specific electrophysiological pattern. Complete, Schubert-Bornschein CSNB is usually transmitted as a monogenic trait, and most familial cases result from mutations of the NYX gene located on the X chromosome. We report a very rare family with consanguineous, first-cousin parents, where a son and a daughter are affected with this condition, indicating autosomal recessive inheritance. As the family was too small for genome-wide linkage, we considered several candidate loci, including the sidekick SDK1 and SDK2 genes. The latter determine lamina-specific connectivity in the retina, a histological substrate of the ON pathway implicated in complete, Schubert-Bornschein CSNB. Although linkage was excluded in our family, observations like the present one may lead to the identification of a new molecular cause for this condition. 相似文献