全文获取类型
收费全文 | 2100篇 |
免费 | 128篇 |
国内免费 | 8篇 |
专业分类
耳鼻咽喉 | 44篇 |
儿科学 | 60篇 |
妇产科学 | 64篇 |
基础医学 | 286篇 |
口腔科学 | 27篇 |
临床医学 | 267篇 |
内科学 | 370篇 |
皮肤病学 | 52篇 |
神经病学 | 185篇 |
特种医学 | 57篇 |
外科学 | 212篇 |
综合类 | 31篇 |
预防医学 | 216篇 |
眼科学 | 31篇 |
药学 | 152篇 |
中国医学 | 9篇 |
肿瘤学 | 173篇 |
出版年
2023年 | 19篇 |
2022年 | 38篇 |
2021年 | 71篇 |
2020年 | 31篇 |
2019年 | 48篇 |
2018年 | 62篇 |
2017年 | 41篇 |
2016年 | 62篇 |
2015年 | 65篇 |
2014年 | 101篇 |
2013年 | 137篇 |
2012年 | 158篇 |
2011年 | 156篇 |
2010年 | 95篇 |
2009年 | 84篇 |
2008年 | 111篇 |
2007年 | 115篇 |
2006年 | 125篇 |
2005年 | 87篇 |
2004年 | 112篇 |
2003年 | 96篇 |
2002年 | 85篇 |
2001年 | 36篇 |
2000年 | 39篇 |
1999年 | 29篇 |
1998年 | 17篇 |
1997年 | 14篇 |
1996年 | 14篇 |
1995年 | 9篇 |
1994年 | 16篇 |
1993年 | 6篇 |
1992年 | 17篇 |
1991年 | 7篇 |
1990年 | 9篇 |
1989年 | 7篇 |
1988年 | 7篇 |
1987年 | 10篇 |
1986年 | 9篇 |
1985年 | 4篇 |
1984年 | 5篇 |
1983年 | 7篇 |
1982年 | 8篇 |
1981年 | 3篇 |
1980年 | 12篇 |
1979年 | 5篇 |
1978年 | 7篇 |
1977年 | 5篇 |
1971年 | 6篇 |
1970年 | 5篇 |
1966年 | 5篇 |
排序方式: 共有2236条查询结果,搜索用时 15 毫秒
61.
Janet L. Kwiatkowski Jill V. Hunter Kim Smith-Whitley Mira L. Katz Justine Shults Kwaku Ohene-Frempong 《British journal of haematology》2003,121(2):375-380
Summary. The risk of stroke in sickle cell disease (SCD) may be influenced by either genetic or environmental factors. Elevated blood flow velocity in the large cerebral arteries, detected by transcranial Doppler (TCD) ultrasonography, predicts an increased stroke risk in children with SCD. We undertook this study to investigate the possibility of a familial predisposition to elevated cerebral blood flow velocity, a surrogate marker for stroke risk. We analysed the results of TCD studies performed on 63 children from 29 families that had more than one child with SCD. We assessed the association of elevated cerebral blood flow velocity with sibling TCD results as well as age and haemoglobin level, which are factors known to affect cerebral blood flow velocity. Positive or negative TCD results were highly correlated between family members ( r = 0·61). The presence of a sibling with a positive TCD result was significantly associated with an elevated cerebral blood flow velocity in other siblings with SCD (odds ratio = 41·9, 95% confidence interval 8·2–214·7, P < 0·001). Furthermore, children who had a sibling with a positive TCD result had a significantly higher TCD velocity than children with SCD but without a sibling who were matched for age, sex, genotype and haemoglobin level. Our results are consistent with a familial predisposition to cerebral vasculopathy in SCD. 相似文献
62.
63.
64.
Management of recurrent and persistent metastatic lymph nodes in well‐differentiated thyroid cancer: A multifactorial decision‐making guide for the thyroid cancer care collaborative 下载免费PDF全文
Mark L. Urken MD Mira Milas MD Gregory W. Randolph MD Ralph Tufano MD Donald Bergman MD Victor Bernet MD Elise M. Brett MD James D. Brierley MD Rhoda Cobin MD Gerard Doherty MD Joshua Klopper MD Stephanie Lee MD PhD Josef Machac MD Jeffrey I. Mechanick MD Lisa A. Orloff MD Douglas Ross MD Robert C. Smallridge MD David J Terris MD Jason B Clain BS Michael Tuttle MD 《Head & neck》2015,37(4):605-614
65.
66.
67.
Yonatan Perez Libe Gradstein Hagit Flusser Barak Markus Idan Cohen Yshaia Langer Mira Marcus Tova Lifshitz Rotem Kadir Ohad S Birk 《European journal of human genetics : EJHG》2014,22(5):703-706
Foveal hypoplasia, always accompanied by nystagmus, is found as part of the clinical spectrum of various eye disorders such as aniridia, albinism and achromatopsia. However, the molecular basis of isolated autosomal recessive foveal hypoplasia is yet unknown. Individuals of apparently unrelated non consanguineous Israeli families of Jewish Indian (Mumbai) ancestry presented with isolated foveal hypoplasia associated with congenital nystagmus and reduced visual acuity. Genome-wide homozygosity mapping followed by fine mapping defined a 830 Kb disease-associated locus (LOD score 3.5). Whole-exome sequencing identified a single missense mutation in the homozygosity region: c.95T>G, p.(Ile32Ser), in a conserved amino acid within the first predicted transmembrane domain of SLC38A8. The mutation fully segregated with the disease-associated phenotype, demonstrating an ∼10% carrier rate in Mumbai Jews. SLC38A8 encodes a putative sodium-dependent amino-acid/proton antiporter, which we showed to be expressed solely in the eye. Thus, a homozygous SLC38A8 mutation likely underlies isolated foveal hypoplasia. 相似文献
68.
Elmeri M. Jokinen Pekka A. Postila Mira Ahinko Sanna Niinivehmas Olli T. Pentikinen 《Chemical biology & drug design》2019,94(4):1799-1812
A novel virtual screening methodology called fragment‐ and negative image‐based (F‐NiB) screening is introduced and tested experimentally using phosphodiesterase 10A (PDE10A) as a case study. Potent PDE10A‐specific small‐molecule inhibitors are actively sought after for their antipsychotic and neuroprotective effects. The F‐NiB combines features from both fragment‐based drug discovery and negative image‐based (NIB) screening methodologies to facilitate rational drug discovery. The selected structural parts of protein‐bound ligand(s) are seamlessly combined with the negative image of the target's ligand‐binding cavity. This cavity‐ and fragment‐based hybrid model, namely its shape and electrostatics, is used directly in the rigid docking of ab initio generated ligand 3D conformers. In total, 14 compounds were acquired using the F‐NiB methodology, 3D quantitative structure–activity relationship modeling, and pharmacophore modeling. Three of the small molecules inhibited PDE10A at ~27 to ~67 μM range in a radiometric assay. In a larger context, the study shows that the F‐NiB provides a flexible way to incorporate small‐molecule fragments into the drug discovery. 相似文献
69.
Huang ZM Chinen M Chang PJ Xie T Zhong L Demetriou S Patel MP Scherzer R Sviderskaya EV Bennett DC Millhauser GL Oh DH Cleaver JE Wei ML 《Proceedings of the National Academy of Sciences of the United States of America》2012,109(2):553-558
Protein-trafficking pathways are targeted here in human melanoma cells using methods independent of oncogene mutational status, and the ability to up-regulate and down-regulate tumor treatment sensitivity is demonstrated. Sensitivity of melanoma cells to cis-diaminedichloroplatinum II (cDDP, cis-platin), carboplatin, dacarbazine, or temozolomide together with velaparib, an inhibitor of poly (ADP ribose) polymerase 1, is increased by up to 10-fold by targeting genes that regulate both protein trafficking and the formation of melanosomes, intracellular organelles unique to melanocytes and melanoma cells. Melanoma cells depleted of either of the protein-trafficking regulators vacuolar protein sorting 33A protein (VPS33A) or cappuccino protein (CNO) have increased nuclear localization of cDDP, increased nuclear DNA damage by platination, and increased apoptosis, resulting in increased treatment sensitivity. Depleted cells also exhibit a decreased proportion of intracellular, mature melanosomes compared with undepleted cells. Modulation of protein trafficking via cell-surface signaling by binding the melanocortin 1 receptor with the antagonist agouti-signaling protein decreased the proportion of mature melanosomes formed and increased cDDP sensitivity, whereas receptor binding with the agonist melanocyte-stimulating hormone resulted in an increased proportion of mature melanosomes formed and in decreased sensitivity (i.e., increased resistance) to cDDP. Mutation of the protein-trafficking gene Hps6, known to impair the formation of mature melanosomes, also increased cDDP sensitivity. Together, these results indicate that targeting protein-trafficking molecules markedly increases melanoma treatment sensitivity and influences the degree of melanosomes available for sequestration of therapeutic agents. 相似文献
70.