降低体重指数对原发性高血压病降压效果的影响及其临床意义

目的探讨降低体重指数(BodyMassIndex,BMI)对原发性高血压病降压效果的影响及其临床意义。方法选择40例超重或肥胖的(BMI≥24)原发性高血压病患者,分为治疗组与对照组,降低治疗组患者的体重指数,同时观察两组治疗前后血压的变化。结果发现治疗组患者降低体重指数后血压有明显下降,与对照组相比有显著的临床意义(P<0.05)。结论降低超重或肥胖的高血压病患者的体重指数可起到一定的降压效果,应注意降低患者的体重指数。     

Vitapex糊剂在儿童年轻恒牙根尖诱导成形术中的应用

目的探讨Vitapex糊剂和氢氧化钙糊剂用于儿童年轻恒牙根尖诱导成形术的治疗效果。方法对85例儿童,98颗牙根尖发育未完成,牙髓病变已波及根髓、牙髓已坏死或并发根尖周炎的年轻恒牙分为2组,分别用Vitapex糊剂和氢氧化钙糊剂进行根尖诱导成形术,观察其根尖发育情况。结果Vitapex糊剂组总有效率为91.84%。氢氧化钙组总有效率为77.55%。2者疗效在统计学比较存在显著性。结论Vitapex糊剂作为一种根尖诱导成形剂具有良好的治疗效果。值得在临床推广应用。     

磁共振技术对新生儿青紫型先天性心脏病的诊断意义

近年来,随着心脏外科的发展,儿童先天性心脏病(简称先心病)的手术年龄越来越小,对于某些先心病如完全性大动脉转位等,在新生儿期手术治疗的效果远比新生儿期后手术治疗效果要好。新生儿先心病外科手术要求术前精确地显示心脏畸形的形态,这就对新生儿心脏影像诊断提出了很高的要求。超声心动图是新生儿心脏影像检查的首选方法,能很好地显示心内结构异常,但对于心外大血管异常和复杂性先心病有时还需要其他影像检查方法作为补充。心血管造影、多层螺旋CT、电子束CT和磁共振检查都能补充超声心动图的不足,但前3种检查方法患儿均需接受较大量…     

Primary cardiac hemangioendothelioma: a case report

Primary cardiac hemangioendothelioma is extremely rare.1-3 Up to now less than twenty cases have been reported in English literature, the data about this kind of cardiac tumors are scanty. In this report, a case of a huge hemangio-endothelioma that arose from the right atrium and was successfully resected is presented.     

Immune responses against SARS-coronavirus nucleocapsid protein induced by DNA vaccine

The nucleocapsid （N） protein of SARS-coronavirus （SARS-CoV） is the key protein for the formation of the helical nucleocapsid during virion assembly. This protein is believed to be more conserved than other proteins of the virus, such as spike and membrane glycoprotein. In this study, the N protein of SARS-CoV was expressed in Escherichia coli DHSalpha and identified with pooled sera from patients in the convalescence phase of SARS. A plasmid pCI-N, encoding the full-length N gene of SARS-CoV, was constructed. Expression of the N protein was observed in COS1 cells following transfection with pCI-N. The immune responses induced by intramuscular immunization with pCI-N were evaluated in a murine model. Serum anti-N immunoglobutins and splenocytes proliferative responses against N protein were observed in immunized BALB/c mice. The major immunoglobulin G subclass recognizing N protein was immunoglobulin G2a, and stimulated splenocytes secreted high levels of gamma interferon and IL-2 in response to N protein. More importantly, the immunized mice produced strong delayed-type hypersensitivity （DTH） and CD^8＋ CTL responses to N protein.     

Frank压迫法阴道成形术治疗先天性无阴道

目的 探讨Frank压迫法阴道成形术治疗先天性无阴道的临床效果.方法 观察Frank法治疗159例先天性无阴道患者的治疗时间和随访治疗效果.结果 159例患者全部治疗成功,所需时间最长7个月,最短 10 d,62例已结婚,随访性生活满意.结论 对于阴道前庭部弹性好,阴道外口处有0.5cm以上陷凹的先天性无阴道患者,用Frank压迫法阴道成形术简便、安全、有效,有2cm以上阴道者,疗效更佳.     

浅析心肌肥厚患者左室舒张功能与冠脉循环的关系

目的探讨心肌肥厚患者冠脉血流与左室舒张功能障碍关系。方法采用彩色多普勒超声仪,行经胸超声心动图(TTE)测量40例心肌肥厚患者和20例正常人冠状窦、二尖瓣及肺静脉血流频谱。结果心肌肥厚患者冠状窦直径、前向血流和净前向血流流速积分(VTInet)明显高于正常对照组;冠状窦前向血流和VTInet与左室舒张功能障碍的多项指标存在相关关系;肺静脉舒张末期逆行血流最大流速之间均存在明显正相关。结论冠脉循环血流动力学的改变可能是心肌肥厚患者舒张功能受损的机制之一.     

不孕不育者对人工授精技术的伦理态度调查研究

目的：研究不孕不育者对人工授精技术的伦理态度,促进该项技术更好地为患者服务.方法：自制问卷调查了223名不孕不育者.结果：不孕不育者对AIH、AID的态度存在显著差异（P＜0.05）;对婚姻家庭生活选择相对宽容;精子的商品化趋向值得关注;必须采取一定措施以保证精子和人工授精儿的质量.结论：AID会引发许多伦理问题,临床应用时更应遵循有利于患者、知情同意等伦理原则和相关法律规定,以减少纠纷,造福社会.     

The up-regulation of preprodynorphin mRNA in trigeminoparabrachial neurons after inflammation

Inflammation of the temporomandibular joint (TMJ) produces an increase in preprodynorphin (PPD) mRNA expression in the spinal trigeminal nucleus (Vsp) and paratrigeminal nucleus (Pa5) of the rat. In this study, we further demonstrated that a portion of the TMJ inflammation-induced PPD mRNA positive neurons in the Vsp and Pa5 projected to the parabrachial nucleus (PB). In inflamed rats, the percentage of trigemino- and paratrigeminoparabrachial neurons with up-regulation of PPD mRNA was significantly increased in the ipsilateral Vsp (5.7+/-1.8%) and Pa5 (22.8+/-7.4%, n = 3) when compared with the contralateral side and with saline-treated controls (p < 0.05). These results suggest that the selective up-regulation of PPD mRNA in the Vsp and Pa5 following TMJ inflammation involves ascending trigeminal nociceptive pathways.     

Taurine inhibition of metal-stimulated catecholamine oxidation

Taurine is an abundant amino acid found in mammalian tissues and it has been suggested to have cyto-protective functions. The aim of the present study was to determine if taurine had the potential to reduce oxidative stress associated with metal-stimulated catecholamine oxidation. Taurine and structural analogs of taurine were tested for their ability to inhibit metal-stimulated quinone formation from dopamine or L-dopa. Oxidative damage to proteins and lipids were also assessedin vitro and the effects of taurine were determined. Taurine (20 mM) was found to decrease significantly ferric iron (50–500 μM)- and manganese (10 μM)-stimulated L-dopa or dopamine oxidation. Taurine had no effect on zinc-induced dopamine oxidation and slightly potentiated copper- and NaIO₄-stimulated quinone formation. Ferric iron-stimulated lipid peroxidation was not affected by taurine (1–20 mM). Protein carbonyl formation induced by ferric iron (500 μM) and L-dopa (500 μM) was significantly reduced by 10 mM taurine. The cytotoxicity of L-dopa (250 μM) and ferric chloride (75 μM) to LLC-PK₁ cells was attenuated by 10 mM taurine or hypotaurine. Homotaurine alone stimulated L-dopa oxidation and potentiated the cytotoxic effects of ferric iron. Homotaurine was found to be cytotoxic when combined with L-dopa or L-dopa/iron. In contrast, hypotaurine inhibited quinone formation and protected LLC-PK₁ cells. These studies suggest that taurine may exhibit cytoprotective effects against the oxidation products of catecholamines by acting as a scavenger for free radicals and cytotoxic quinones.