Functional analysis of SEMA3A variants identified in Chinese patients with isolated hypogonadotropic hypogonadism

Isolated hypogonadotropic hypogonadism (IHH) is a rare disorder characterized by impaired sexual development and infertility, caused by the deficiency of hypothalamic gonadotropin-releasing hormone neurons. IHH is named Kallmann's syndrome (KS) or normosmic IHH (nIHH) when associated with a defective or normal sense of smell. Variants in SEMA3A have been recently identified in patients with KS. In this study, we screened SEMA3A variants in a cohort of Chinese patients with IHH by whole exome sequencing. Three novel heterozygous SEMA3A variants (R197Q, R617Q and V458I) were identified in two nIHH and one KS patients, respectively. Functional studies indicated that R197Q and R617Q variants were ineffective in activating the phosphorylation of FAK (focal adhesion kinase) in GN11 cells, despite normal production and secretion in HEK293T cells. The V458I SEMA3A had defect in secretion as it was not detected in the conditioned medium from HEK293T cells. Compared with wild type SEMA3A protein, all three SEMA3A mutant proteins were ineffective in inducing the migration of GN11 cells. Our study further showed the contribution of SEMA3A loss-of-function variants to the pathogenesis of IHH.     

特需病房护理信息系统应用中的问题分析及对策

目的对特需病房护理信息系统应用中出现的问题进行总结,探讨解决方法。方法2017年1—2月总结特需病房护理信息系统应用中的问题并分析原因,2017年3月起实施改进措施,包括:加强护士培训和质量控制、与医生密切合作、增强对医嘱的审核能力、双人核对医嘱、文字交接班、建立病房口服药品登记等。比较改进前后的护理质量相关指标。结果实施改进措施后,护士每日电脑操作耗时减少,护士发现及纠正问题医嘱的数量增加,给药错误防范次数增加,差异有统计学意义(P<0.05)。结论对特需病房护理信息系统实施改进措施有效提高了护士工作效率,预防和减少护理差错发生。     

经阴道超声评价不孕症患者子宫内膜容受性的研究进展

随着辅助生殖技术的发展,应用体外受精一胚胎移值（IVF-EI）对小孕症患者的诊治取得了很大的进展,胚胎质量和子宫内膜容受性是决定不孕症患着治疗成败的两个关键因素,用于评价子宫内膜的方法很多,如免疫组化法、内膜活检等,而经阴道超声以其简便、无创、可重复性等优点成为不孕症诊治中评估子宫内膜容受性的常规方法。     

二维血流显像技术与彩色多普勒在颈动脉疾病诊断中的应用比较

目的比较二维血流显像技术（B-Flow）与彩色多普勒血流显像（CDFI）和能量多普勒（PDI）在颈动脉疾病诊断中的应用价值。方法对160例颈动脉疾病患者分别进行CDFI、PDI及B-Flow技术检查,对比分析。其中41例患者进行了数字减影血管造影（DSA）检查。结果B-Flow技术是一种新型血管疾病影像学检查方法,具有更高帧频、高分辨率和全景显像功能,能同时显示血流和背景组织结构,B-Flow与CDFI、PDI结合使用,可提高异常图像显示率;对于颈动脉狭窄和闭塞的判断与DSA结果比较,B-Flow技术与DSA相关性明显高于后两者。结论B-Flow显像技术较CDFI及PDI显像在颈动脉疾病中的应用范围更广,可以弥补CDFI、PDI显像上的不足。     

胰腺癌细胞通过高表达核因子E2相关因子2促进巨噬细胞血管内皮生长因子表达的实验研究

目的:探讨胰腺癌细胞对巨噬细胞血管内皮生长因子(VEGF)表达的影响及核因子E2相关因子2(Nrf2)在其中的作用。方法:检测单一人胰腺癌细胞(PANC-1)培养组、单一巨噬细胞培养组、肿瘤细胞和巨噬细胞共培养组中各细胞VEGF表达。利用野生组、Nrf2过表达组、Nrf2敲减组中PANC-1的条件培养基刺激巨噬细胞,检...     

Preparation of rGO@Fe3O4 nanocomposite and its application to enhance the thermal conductivity of epoxy resin

In this study, we report a simple method to improve the thermal conductivity of epoxy resin by using new magnetic composites as fillers. The rGO@Fe₃O₄ nanocomposite has been prepared by a solvothermal method, and its morphology and chemical structure were characterized and analyzed by various characterization methods. Afterwards, the rGO@Fe₃O₄/EP composite material was obtained in an external magnetic field, in which the rGO@Fe₃O₄ is uniformly dispersed in the epoxy resin matrix, arranged along the direction of the magnetic field. In addition, the orientation of rGO@Fe₃O₄ increases with the magnetic field intensity. After doping 30% (wt) rGO@Fe₃O₄ into epoxy resin and curing under a 500 Gs magnetic field, the rGO@Fe₃O₄/EP composite material is anisotropic and has a higher thermal conductivity (increased by 196.60%) parallel to the direction of the magnetic field compared to a pure ring oxygen resin.

A rGO@Fe₃O₄ nanocomposite was prepared by a solvothermal method. Then the rGO@Fe₃O₄/epoxy resin composite was cured in a magnetic field. This shows that rGO@Fe₃O₄ can enhance the heat-conductivity of epoxy resins.     

ⅢA-N2期非小细胞肺癌新辅助治疗的研究现状

ⅢA-N2期非小细胞肺癌(NSCLC)是一组异质性较大的疾病,最佳治疗模式仍存在争议。如何将化疗、放疗、靶向及免疫治疗等多种治疗手段合理的应用于这部分患者的新辅助治疗中,目前尚无定论。本文就NSCLC新辅助治疗相关研究进行综述,对不同新辅助治疗模式进行疗效及安全性方面的评估。