The role of the complement system in the defence against infections and new ways of assessing the activation routes

The complement system is of major importance in the defence against infections. Deficiencies in one of the three pathways of activation of the complement system can cause serious infections; the classical pathway, the alternative pathway and the lectin pathway. A new ELISA assay has been developed to enable the assessment of the functional activity of the whole lectin pathway and to determine the classical and the alternative pathway functional activity in a simple uniform design.     

Physical activity in daily life in patients with chronic low back pain

OBJECTIVES: To evaluate disuse (ie, a decreased daily physical activity level) in patients with chronic low back pain (LBP) and to evaluate the construct validity of accelerometry for measuring physical activity in daily life. DESIGN: Case-control study in a cross-sectional design; comparison of accelerometry to the criterion standard (doubly labeled water technique). SETTING: Normal daily living (unrestricted by the measurement devices). PATIENTS: Thirteen patients with chronic nonspecific LBP and 13 age- and gender-matched healthy controls. MAIN OUTCOME MEASURES: Physical activity in daily life, expressed as whole-body acceleration measured with a triaxial accelerometer (Tracmor), and as the ratio between average daily metabolic rate (ADMR), measured by the doubly labeled water technique, and resting metabolic rate (RMR), measured by the ventilated hood. Both techniques were used simultaneously for 14 days. RESULTS: Mean physical activity level in patients and controls did not differ significantly. The correlation between the Tracmor and ADMR and RMR was .72 (p < .01). CONCLUSIONS: Decreased physical activity levels in this sample of chronic LBP patients was not confirmed. The Tracmor is a valid instrument for measuring daily activity in LBP patients.     

Chair configuration and balance control in persons with spinal cord injury

OBJECTIVE: To study whether chair configuration influences sitting balance in persons with spinal cord injury (SCI). DESIGN: Cross-sectional group study. SETTING: Rehabilitation centers and hospital rehabilitation departments. PATIENTS: Ten complete high thoracic SCI (level T2-T8) patients, 10 complete low thoracic SCI (T9-T12) patients, and 10 matched able-bodied controls. SCI participants had completed their active rehabilitation at least 6 months before the study. INTERVENTIONS: A balance-changing (forward) reaching task while seated in four differently configured chairs. Tilt angle (7 degrees and 12 degrees) and reclination angle (22 degrees) were varied relative to a standard chair configuration (10 degrees reclination). MAIN OUTCOME MEASURES: Maximal unsupported reaching distance, center-of-pressure displacement and muscle activity. RESULTS: Although no significant difference in actively controllable reach was found in controls or in subjects with low SCI, sitting balance improved in all chairs relative to the standard chair. Ability to control displacement of arms and trunk during reaching improved. No apparent need for additional postural muscle activity was found. Persons with high SCI did not improve their sitting balance. They were unable to control a shift in body mass larger than the one induced by arm movement. However, they had less muscle activity after backrest reclination or tilting the chair backwards. CONCLUSIONS: The tested chairs had an overall positive effect. However, for individually tailored chair configurations factors other than those investigated should be considered.     

Complement and renal disease

The complement system is involved in several aspects of renal disease, including primary renal diseases, dialysis and renal transplant rejection. Initially, a role for complement in renal disease was inferred solely from the deposition of complement components in affected kidneys and changes in complement levels in the circulation during active disease. Recent studies have shown how complement modulates the onset, development and the resolution of renal disease. Current research provides clues on the role of individual complement components and activation pathways as well as possible modes of complement regulation in the management of renal disease.     

Eosinophilic enteritis presenting as acute abdomen: US features of two cases.

Eosinophilic enteritis is a rare disease which may mimic acute abdominal emergency. Two sonographically documented cases are presented, which were subsequently proven at operation. Although the sonographic features of severe echolucent bowel wall thickening were not specific, combination with clinical and laboratory data may suggest the correct diagnosis.     

Subjective complaints and blood pressure

The relationship between blood pressure and subjective complaints concerning physical well-being has been investigated. The complaints have been measured by means of the Inventory of Subjective Health. The total number of complaints are the summation of 39 items.Hypertension is assumed to have no clear-cut symptoms. This statement gives rise to the hypothesis that there is no relationship between blood pressure and subjective complaints. The hypothesis was refuted after several tests. We found a curvilinear relationship between diastolic blood pressure and subjective complaints in representative groups of men of 21–65 and 31–50 years old, valid for the total number of complaints and dizziness. This curvilinear relationship is found again after a matching procedure in order to exclude possible confounding effects of age, cigarette smoking and knowledge about elevated blood pressure status.The total number of complaints of males with a diastolic pressure higher than 95 mmHg are not significantly higher than the total number of complaints of males with a lower diastolic pressure, but the difference is significant for dizziness.     

Glomerular deposition of C1q and anti-C1q antibodies in mice following injection of antimouse C1q antibodies

Anti-C1q autoantibodies are present in the serum of patients with different autoimmune diseases such as systemic lupus erythematosus (SLE). The occurrence of these autoantibodies correlates with renal involvement. In the present study we examined whether injection of rabbit antimouse C1q antibodies in mice leads to deposition in kidneys. Injection of healthy mice with a single dose of rabbit IgG antimouse C1q antibodies resulted in deposition of both C1q and IgG anti-C1q in glomeruli. The pattern of deposition observed in the glomeruli of mice injected with antimouse C1q antibodies both at 24 h and 2 weeks was both glomerular basement membrane (GBM)-associated and mesangial. Injection of control IgG did not have a detectable effect on circulating C1q levels, and no deposition of either C1q or rabbit IgG was seen at 24 h. The deposition of rabbit antimouse C1q and C1q in glomeruli resulted in complement activation, as assessed by C3 deposition, and influx of leucocytes associated with albuminuria in some, but not all mice. In none of the control mice was albuminuria observed. This report is the first to show that anti-C1q antibodies deposit in the healthy glomerulus together with autologous C1q. This deposition is stable for at least 2 weeks, causes complement activation, leucocyte influx and can lead to mild albuminuria.     

The role of the lateral suprasylvian visual cortex of the cat in object-background interactions: Permanent deficits following lesions

The contribution of the lateral suprasylvian cortex to pattern recognition was studied by behavioural detection experiments in combination with bilateral lesions of different parts of the lateral suprasylvian areas (LSA) and area 7 in seven cats. In a two-alternatives forced choice task the cats had to discriminate simple outline patterns which were additively superimposed on a structured visual background made up of broadband Gaussian noise. For various stimulus conditions (moving or stationary patterns and/or background) the detection probability (P _D) of the cats was measured as a function of the signal to noise ratio (S/N). Each cat was tested before and after the lesion. Four different types of lesion could be distinguished depending on their extent: (1) lesion of parts of the (LSA); (2) lesion of parts of the LSA with undercutting of areas 17, 18 and 19; (3) lesion of area 7; (4) lesion of area 7 and parts of the LSA.