Recombinant rat stem cell factor synergizes with recombinant human granulocyte colony-stimulating factor in vivo in mice to mobilize peripheral blood progenitor cells that have enhanced repopulating potential

Splenectomized mice treated for 7 days with pegylated recombinant rat stem cell factor (rrSCF-PEG) showed a dose-dependent increase in peripheral blood progenitor cells (PBPC) that have enhanced in vivo repopulating potential. A dose of rrSCF-PEG at 25 micrograms/kg/d for 7 days produced no significant increase in PBPC. However, when this dose of rrSCF-PEG was combined with an optimal dose of recombinant human granulocyte colony-stimulating factor (rhG-CSF; 200 micrograms/kg/d), a synergistic increase in PBPC was observed. Compared with treatment with rhG-CSF alone, the combination of rrSCF-PEG plus rhG-CSF resulted in a synergistic increase in peripheral white blood cells, in the incidence and absolute numbers of PBPC, and in the incidence and absolute numbers of circulating cells with in vivo repopulating potential. These data suggest that low doses of SCF, which would have minimal, if any, effects in vivo, can synergize with optimal doses of rhG-CSF to enhance the mobilization of PBPC stimulated by rhG-CSF alone.     

深部经颅磁刺激对抑郁症患者大脑自发神经活动的影响

目的 探讨深部经颅磁刺激（dTMS）对抑郁症患者大脑自发神经活动的影响。方法 选取2022 年 9 月— 2024 年 1 月于河北医科大学第一医院精神卫生中心门诊或住院治疗的 17 例抑郁症患者为研究组,选取 2021 年 9 月— 2023 年 8 月于河北医科大学第一医院健康体检中心体检的 10 名健康者为对照组。研究组使用 dTMS 刺激左侧背外侧前额叶皮质,频率为 18 Hz,刺激强度为静息运动阈值的 120%;每周治疗 5 次,连续治疗 2 周,共计 10 次。收集受试者一般资料。采用汉密尔顿抑郁量表（HAMD）、汉密尔顿焦虑量表（HAMA）评估研究组患者治疗前后的抑郁及焦虑情况,并采集受试者头颅静息态功能核磁共振（rs-fMRI）图像。使用分数低频振幅（fALFF）评估研究组患者治疗前后及研究组治疗前与对照组的大脑自发神经活动变化。采用t检验或非参数检验评估研究组治疗前后的大脑自发神经活动变化,并采用 Pearson 相关分析临床症状与脑区自发神经活动的相关性。结果 最终纳入研究组 16 例,对照组10名。研究组治疗后的HAMA总分、HAMD总分及各因子评分均低于治疗前,差异有统计学意义（P＜0.05）。研究组治疗前的右中央旁小叶 fALFF 值低于对照组,差异有统计学意义（t=5.36,P＜ 0.05）。研究组经 dTMS 治疗后的左侧额中回、右内侧额上回 fALFF 值降低,右侧舌回、左侧舌回、右侧枕中回、右中央旁小叶 fALFF 值升高,差异均有统计学意义（t=12.36、16.01、-11.07、-10.81、-10.19、-14.14,P＜0.05）。Pearson相关分析显示,治疗前的左侧舌回fALFF值与HAMA总分（r=-0.89,P=0.017）、焦虑/躯体因子得分（r=-0.96,P=0.002）呈负相关,治疗后左侧额中回 fAFLL 值下降与迟滞因子得分下降呈正相关（r=0.88,P=0.021）。结论 抑郁症患者的额叶、舌叶和枕叶存在异常的自发神经活动,dTMS 治疗后自发神经活动的平衡与抑郁焦虑和迟滞症状的改善有关。     

童年期创伤与静息态血压、心率及心血管疾病的相关性研究进展

童年期创伤与静息态血压及心率存在着一定的关联性,是预测心血管疾病的重要风险因素。本文通过总结童年期创伤与静息态血压、心率和心血管疾病之间的相关性以及童年期创伤影响静息态血压、心率及心血管疾病的潜在机制,包括表观遗传学、神经内分泌、氧化应激、炎性反应、不良生活方式、心理应激等,明确童年期创伤对心血管系统的潜在影响,旨在为早期防治心血管疾病提供理论依据。     

组织工程化人形下颌骨髁状突的实验研究

目的　评价采用组织工程技术构建人下颌骨髁状突软骨复合组织的可行性。方法　以可降解生物材料聚羟基乙酸 (polyglycolicacid ,PGA) /聚乳酸 (polylacticacid ,PLA)预制人下颌骨髁状突模型 ,体外培养扩增牛骨膜成骨细胞和牛关节表面软骨。实验分 3组 :第 1组 ,模型内仅注入 1.5 %藻酸钙和 30 %氧化乙丙烯 ;第 2组 ,模型内分别注入成骨细胞和软骨细胞悬液 ,浓度为 5× 10 7/ml;第 3组 ,模型内接种成骨细胞藻酸钙悬液 ,同时在模型关节表面涂敷软骨细胞 PluronicF12 7悬液 ,浓度为 5× 10 7/ml。分别植入裸鼠皮下 ,12周后取材 ,作大体和组织学观察。结果　第 1组无骨和软骨形成 ,模型支架明显缩小 ,形态不规则 ;第 2组仅留残缺不全的组织结构 ,标本体积缩小变形 ;第 3组形态结构与原植入模型支架相同 ,组织学观察有骨和软骨组织结构 ,两者界面连接有序 ,骨组织内有大量新生骨板。结论　以人工合成生物材料预制人下颌骨髁状突支架 ,采用组织工程技术 ,可以在裸鼠体内构建具有骨软骨复合结构的正常形态的人形下颌骨髁状突 ,从而为进一步临床应用提供了理论依据     

应用组织工程化皮肤修复皮肤全层缺损

目的本研究旨在评价组织工程技术修复皮肤缺损的可行性。方法采用Ｙｏｒｋｓｈｉｒｅ猪,在其背部脊柱两侧做６个直径４ｃｍ的圆形全厚皮肤缺损,随机分为３组。第１组为空白对照,第２组仅置无细胞的ｐｌｕｒｏｎｉｃ;第３组分别用角朊细胞－ｐｌｕｒｏｎｉｃ、成纤维细胞－ｐｌｕｒｏｎｉｃ复合物置于创面上。第４、６周时取材,进行大体,组织学和透射电镜观察。结果４－６周时表皮和真皮间界面明显被上皮嵴和真皮乳头所分隔。对     

脑卒中患者对负性情绪及心理治疗认知状况的调查

目的:调查甘肃省脑卒中患者对负性情绪是否影响肢体功能以及心理治疗的认知.方法:采用问卷调查方式,对甘肃省10家不同级别的省市县级医院神经内、外科2010年收治的脑卒中住院患者1 100例进行调查.结果:负性情绪对肢体功能恢复的影响占69.80%,脑卒中后心理治疗的需要占76.99%.结论:负性情绪对脑卒中患者肢体功能恢复有影响,并且非常需要心理治疗.     

国内麻醉科医师应用二氧化碳吸收剂的现状调查

目的 调查国内麻醉科医师应用二氧化碳(CO2)吸收剂的现状,为统一CO2吸收剂更换的指标、限值和流程提供参考.方法 采用自主设计调查问卷开展网络问卷调查,主要内容包括麻醉科医师的基本情况、对CO2吸收剂应用理论的理解和CO2吸收剂临床应用的现状.结果 本次调查发放并回收有效问卷475份,其中49.9％来自华南地区,其他...     

Thromboelastometry and organ failure in trauma patients: a prospective cohort study

Introduction

Data on the incidence of a hypercoagulable state in trauma, as measured by thromboelastometry (ROTEM), is limited and the prognostic value of hypercoagulability after trauma on outcome is unclear. We aimed to determine the incidence of hypercoagulability after trauma, and to assess whether early hypercoagulability has prognostic value on the occurrence of multiple organ failure (MOF) and mortality.

Methods

This was a prospective observational cohort study in trauma patients who met the highest trauma level team activation. Hypercoagulability was defined as a G value of ≥11.7 dynes/cm² and hypocoagulability as a G value of <5.0 dynes/cm². ROTEM was performed on admission and 24 hours later.

Results

A total of 1,010 patients were enrolled and 948 patients were analyzed. Median age was 38 (interquartile range (IQR) 26 to 53), 77% were male and median injury severity score was 13 (IQR 8 to 25). On admission, 7% of the patients were hypercoagulable and 8% were hypocoagulable. Altogether, 10% of patients showed hypercoagulability within the first 24 hours of trauma. Hypocoagulability, but not hypercoagulability, was associated with higher sequential organ failure assessment scores, indicating more severe MOF. Mortality in patients with hypercoagulability was 0%, compared to 7% in normocoagulable and 24% in hypocoagulable patients (P <0.001). EXTEM CT, alpha and G were predictors for occurrence of MOF and mortality.

Conclusions

The incidence of a hypercoagulable state after trauma is 10% up to 24 hours after admission, which is broadly comparable to the rate of hypocoagulability. Further work in larger studies should define the clinical consequences of identifying hypercoagulability and a possible role for very early, targeted use of anticoagulants.

Electronic supplementary material

The online version of this article (doi:10.1186/s13054-014-0687-6) contains supplementary material, which is available to authorized users.