缺氧诱导因子1α通过上调Snail促进HepG2肝癌细胞上皮-间充质化

目的上皮-间充质化（epithelial-mesenchymal transition,EMT）是实体瘤原发灶癌细胞获得转移能力的基础。缺氧诱导前列腺癌、肾癌、卵巢癌的EMT过程已得到证实,缺氧诱导因子1α（hypoxia inducible factor 1 alpha,HIF-1α）在这些过程中发挥重要作用。但是HIF-1α和肝癌细胞EMT之间的关系目前并不清楚。本文探讨HIF-1α在肝癌EMT中的作用。方法利用可调控HIF-1α表达的肝癌HepG2Tet-on-HIF-1α细胞系,在排除缺氧其他反应干扰的情况下研究HIF-1α在肝癌细胞EMT过程中的作用和机制。结果过表达HIF-1α促进HepG2肝癌细胞EMT,下调HIF-1α表达可以抑制HepG2肝癌细胞EMT。HIF-1α促进EMT相关转录因子Snail的表达。结论 HIF-1α通过上调Snail来促进HepG2肝癌细胞EMT。     

Cationic liposome-mediated enhanced generation of human HLA-restricted RSV-specific CD8+ CTL+

Generation of human CD8⁺ cytotoxic T-lymphocyte (CTL) activity against respiratory syncytial virus (RSV) using peripheral blood leukocytes (PBL) in vitro is inefficient. Lipofectamine, a polycationic liposome, previously shown to enhance the transfection efficiency of DNA in cells, was evaluated for enhancing RSV CTL activity. Stimulator cells were prepared by infecting human PBL with RSV with or without Lipofectamine for 3 hr and then transferred to responder cells. After 8 days of incubation, CTL lysis of autologous target cells infected with RSV (also treated with Lipofectamine) was determined in a 4-hr ⁵¹chromium release assay. Lipofectamine treatment significantly enhanced HLA-restricted RSV-specific CD8⁺ CTL activity (up to sevenfold, P < 0.05–0.001). Lipofectamine treatment also enhanced cell surface RSV antigen expression and increased the frequencies of HLA-A,B,C⁺/RSV⁺ and HLA-DR⁺/RSV⁺ leukocytes as demonstrated by flow cytometry. These results demonstrate the usefulness of cationic liposomes in augmenting cell surface antigen expression and increasing the efficiency of generation of human RSV-specific CD8⁺ CTL activity.     

类消胶囊治疗类风湿关节炎的临床观察与实验研究

目的 研究类消胶囊对类曲湿性关节炎的治疗作用。方法 对３０例类风湿关节炎患者服用类消胶囊的后进行血清ＮＰＴ－２Ｒ水平测定。３００你储血员作为对照组,测得ＮＰＴ≤１００ｍｇ／ｍｌ、Ｈ、２Ｒ≤２５０ｕ／ｍｌ。结果 服前药ＮＰＴ与ＩＬ－２Ｒ水平明显高于对照组（Ｐ〈０．００１）。服药后ＮＰＴ与ＩＬ－２Ｒ测定其平均值明显低于有取药前（Ｐ〈０．００１）。结论 类消胶囊可降低ＮＰＴ和ＩＬ－２Ｒ,而ＮＰＴ和ＩＬ－     

超滤工艺对风痛宁注射液中有效成分的影响

目的：采用正交试验方法,研究超滤工艺对风痛宁注射液有效成分的影响。方法：以温度、pH值、药液浓度和超滤膜截留分子量4因素进行正交设计,盐酸青藤碱为检测指标,测定超滤前后药液成分浓度,通过计算透过率来观察盐酸青藤碱超滤前后的含量变化。结果：温度、pH值、药液浓度和超滤膜截留分子量4个考察因素对盐酸青藤碱透过率无显著影响。结论：风痛宁注射液有效成分进行超滤的适用性较好。     

Assessment of olfactory and gustatory functions in COVID-19 patients

Background/aimThis study aims to evaluate of olfactory and gustatory functions of COVID-19 patients and possible risk factors for olfactory and gustatory dysfunctions. Materials and methodsThe cross-sectional study included adult patients who were diagnosed with COVID-19 in Gazi University Hospital between April 2020 and June 2020. Volunteered patients participated in a survey in which olfactory and gustatory functions and various clinical information were questioned. Sinonasal Outcome Test-22 was also administrated to all patients.ResultsA hundred and seventy-one patients participated in this study. Olfactory and gustatory dysfunctions rates were 10.5% (n: 18) and 10.5% (n: 18), respectively. Patients without any symptom other than smell and taste dysfunctions were clustered as group 1 and patients who are clinically symptomatic were clustered as group 2. Olfactory dysfunction occurred in 8% of group 1 and 17.4% of group 2 (p = 0.072). Gustatory dysfunction rate of smokers was 19.7% and significantly higher than gustatory dysfunction rate of nonsmokers (5.5%) (p = 0.007). Twenty-seven-point-eight percent of the patients with olfactory dysfunction (n = 5) were male and 72.2% (n: 13) were female. Sex did not show significant effect on rate of olfactory dysfunction. Twenty-five patients participated in psychophysical olfactory function test. No participant reported olfactory dysfunction at the time of test. Of the participants, 64% (n: 16) were normosmic and 36% (n: 9) were hyposmic according to Sniffin’ Stick test. ConclusionOlfactory and gustatory dysfunctions are more common in patients who are clinically symptomatic than those diagnosed during contact tracing. Objective tests may show that frequency of olfactory dysfunction is greater than frequency of self-reported olfactory dysfunction.     

Long-term proton pump inhibitor use is a risk factor for mortality in patients hospitalized for COVID-19

Background and aimThe aim of this study is to evaluate whether the long-term (≥4 weeks) use of proton pump inhibitors (PPIs) is a risk factor for intubation requirement and mortality in patients hospitalized for COVID-19.Materials and methods In this multicentric retrospective study, a total of 382 adult patients (≥18 years of age) with confirmed COVID-19 who were hospitalized for treatment were enrolled. The patients were divided into two groups according to the periods during which they used PPIs: the first group included patients who were not on PPI treatment, and the second group included those who have used PPIs for more than 4 weeksResultsThe study participants were grouped according to their PPI usage history over the last 6 months. In total, 291 patients did not use any type of PPI over the last 6 months, and 91 patients used PPIs for more than 4 weeks. Older age (HR: 1.047, 95% CI: 1.026–1.068), current smoking (HR: 2.590, 95% CI: 1.334–5.025), and PPI therapy for more than 4 weeks (HR: 1.83, 95% CI: 1.06–2.41) were found to be independent risk factors for mortalityConclusionThe results obtained in this study show that using PPIs for more than 4 weeks is associated with negative outcomes for patients with COVID-19. Patients receiving PPI therapy should be evaluated more carefully if they are hospitalized for COVID-19 treatment.     

磁驱动血泵溶血分析

测试磁驱动轴流心室辅助装置主体血泵溶血性能。利用计算流体力学(CFD)软件ANSYS,基于红细胞受到切应力和相应曝光时间的计算溶血方法预测血泵溶血性能,计算红细胞粒子随着时间推移在血泵内运动轨迹上受到破坏程度。通过体外模拟循环实验实际测试血泵体外溶血性能,计算得到血泵实际标准溶血指数。CFD计算结果转化的标准溶血指数与实际体外实验结果比较相差较大,与CFD计算简化和实际计算循环周期有很大关系。磁驱动轴流心室辅助装置主体血泵有较好的实际溶血性能,血泵实验期间无不良状况发生,可以进行进一步实验。     

Relationships of sleep duration with sociodemographic and health‐related factors,psychiatric disorders and sleep disturbances in a community sample of Korean adults

The aim of this study is to examine relationships of sleep duration with sociodemographic and health‐related factors, psychiatric disorders and sleep disturbances in a nationwide sample in Korea. A total of 6510 subjects aged 18–64 years participated in this study. Logistic regression was used to calculate the odd ratios and 95% confidence intervals of the covariates, psychiatric disorders and sleep disturbances across the following sleep duration categories: 5 h or less, 6, 7, 8 and 9 h or more per day. Low levels of education, unemployment and physical illness were associated with sleeping for 5 h or less and 9 h or more. Being older and widowed/divorced/separated, high levels of physical activity, pain/discomfort, obesity and high scores on the General Health Questionnaires were associated with sleeping for 5 h or less. Female, being younger and underweight were associated with sleeping for 9 h or more. Alcohol dependence, anxiety disorder and social phobia were associated significantly with sleeping for 5 h or less and 9 h or more. Other psychiatric disorders were more common in subjects who slept for 5 h or less (e.g. alcohol use disorder, mood disorder, major depressive disorder, dysthymic disorder, obsessive‐compulsive disorder and specific phobia) or 9 h or more (e.g. post‐traumatic stress disorder). In addition, subjects who slept for 5 h or less reported more sleep disturbances than did subjects who slept for 7 h. Short or long sleep is associated with psychiatric disorders and/or sleep disturbance, therefore attention to the mental health of short or long sleepers is needed.