全文获取类型
收费全文 | 11764篇 |
免费 | 644篇 |
国内免费 | 46篇 |
专业分类
耳鼻咽喉 | 91篇 |
儿科学 | 319篇 |
妇产科学 | 174篇 |
基础医学 | 1444篇 |
口腔科学 | 294篇 |
临床医学 | 885篇 |
内科学 | 2958篇 |
皮肤病学 | 328篇 |
神经病学 | 655篇 |
特种医学 | 311篇 |
外科学 | 1946篇 |
综合类 | 48篇 |
预防医学 | 347篇 |
眼科学 | 275篇 |
药学 | 960篇 |
中国医学 | 41篇 |
肿瘤学 | 1378篇 |
出版年
2023年 | 55篇 |
2022年 | 112篇 |
2021年 | 211篇 |
2020年 | 121篇 |
2019年 | 187篇 |
2018年 | 234篇 |
2017年 | 153篇 |
2016年 | 238篇 |
2015年 | 238篇 |
2014年 | 274篇 |
2013年 | 392篇 |
2012年 | 599篇 |
2011年 | 612篇 |
2010年 | 369篇 |
2009年 | 307篇 |
2008年 | 582篇 |
2007年 | 617篇 |
2006年 | 596篇 |
2005年 | 616篇 |
2004年 | 594篇 |
2003年 | 566篇 |
2002年 | 580篇 |
2001年 | 464篇 |
2000年 | 444篇 |
1999年 | 400篇 |
1998年 | 134篇 |
1997年 | 119篇 |
1996年 | 113篇 |
1995年 | 98篇 |
1994年 | 100篇 |
1993年 | 77篇 |
1992年 | 237篇 |
1991年 | 209篇 |
1990年 | 193篇 |
1989年 | 205篇 |
1988年 | 162篇 |
1987年 | 175篇 |
1986年 | 135篇 |
1985年 | 145篇 |
1984年 | 96篇 |
1983年 | 80篇 |
1979年 | 67篇 |
1978年 | 43篇 |
1977年 | 37篇 |
1975年 | 36篇 |
1974年 | 36篇 |
1972年 | 43篇 |
1971年 | 46篇 |
1970年 | 36篇 |
1969年 | 37篇 |
排序方式: 共有10000条查询结果,搜索用时 0 毫秒
991.
992.
Role of antioxidant defenses against ethanol-induced damage in cultured rat gastric epithelial cells 总被引:1,自引:0,他引:1
Hiraishi H Shimada T Ivey KJ Terano A 《The Journal of pharmacology and experimental therapeutics》1999,289(1):103-109
Reactive oxygen species appears to be involved in the pathogenesis of ethanol-induced gastric mucosal injury in vivo. Because ingested ethanol diffuses into the gastric mucosa, targeting both epithelium and endothelium, in the present study we examined the possible protective effect of antioxidants on ethanol damage in gastric epithelial cells and endothelial cells in vitro. Cytotoxicity by ethanol was quantified by measuring 51Cr release. The effects of impairment of the glutathione redox cycle and of inhibition of cellular catalase were examined. The generation of superoxide was assessed by the reduction in cytochrome c. Ethanol caused a time- and dose-dependent increase in 51Cr release from epithelial cells. Incubation of cells with DL-buthionine-(S,R)-sulfoximine, while reducing glutathione production, dose dependently enhanced ethanol-induced injury. 1,3-Bis(chloroethyl)-nitrosourea, while inhibiting glutathione reductase activity, also sensitized cells to ethanol. In contrast, the inhibition of catalase with 3-amino-1,2, 4-triazole did not alter the susceptibility of epithelial cells to ethanol. Ethanol induced damage to endothelial cells in a similar fashion. In endothelial cells, however, neither impairment of the glutathione cycle nor inhibition of catalase influenced ethanol-induced damage. Epithelial cells, when exposed to ethanol, increased superoxide production as a function of ethanol concentration, whereas endothelial cells did not. The glutathione redox cycle, but not cellular catalase, plays a critical role in protecting epithelial cells against ethanol damage, whereas neither antioxidant seems to play a role in protection of endothelial cells. The distinct difference in antioxidant protection against ethanol appears to depend on the capability of each cell to produce cytotoxic oxygen species in response to ethanol exposure. 相似文献
993.
994.
995.
996.
Role of host angiotensin II type 1 receptor in tumor angiogenesis and growth 总被引:12,自引:0,他引:12
下载免费PDF全文
![点击此处可从《The Journal of clinical investigation》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Egami K Murohara T Shimada T Sasaki K Shintani S Sugaya T Ishii M Akagi T Ikeda H Matsuishi T Imaizumi T 《The Journal of clinical investigation》2003,112(1):67-75
Although the renin angiotensin system (RAS) is a major regulator of vascular homeostasis, the role of the RAS in tumor angiogenesis is little understood. Here we show that host angiotensin II (ATII) type 1 (AT1) receptor plays an important role in angiogenesis and growth of tumor cells engrafted in mice. Subcutaneous B16-F1 melanoma-induced angiogenesis as assessed by tissue capillary density and microangiography was prominent in WT mice but was reduced in AT1a receptor-deficient (AT1a-/-) mice. Consequently, tumor growth rate was significantly slower, and the mouse survival rate was greater, in AT1a-/- mice than in WT mice. Tumor growth was also reduced in WT mice treated with TCV-116, a selective blocker of AT1 receptor. Because the beta-galactosidase gene was inserted into the AT1a gene locus in AT1a-/- mice, the site of beta-galactosidase expression represents the AT1a receptor expression in these mutant mice. In tumor-implanted AT1a-/- mice, the major site of the beta-galactosidase expression was macrophages in tissues surrounding tumors. Moreover, the number of infiltrated macrophages was significantly lower in AT1a-/- mice than in WT mice, and double-immunofluorescence staining revealed that these macrophages expressed VEGF protein intensively. Therefore, the host ATII-AT1 receptor pathway supports tumor-associated macrophage infiltration, which results in enhanced tissue VEGF protein levels. The host ATII-AT1 receptor pathway thereby plays important roles in tumor-related angiogenesis and growth in vivo. 相似文献
997.
Influence of circadian-stage-dependent dosing schedule on nephrotoxicity and pharmacokinetics of isepamicin in rats. 总被引:1,自引:2,他引:1
下载免费PDF全文
![点击此处可从《Antimicrobial agents and chemotherapy》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Y Yoshiyama S Nishikawa T Sugiyama T Kobayashi H Shimada F Tomonaga S Ohdo N Ogawa S Nakano 《Antimicrobial agents and chemotherapy》1993,37(9):2042-2043
Nephrotoxicity was more marked in rats receiving isepamicin at midlight than at middark. And, the once-daily administration at middark induced a lesser degree of nephrotoxicity than the twice-daily injection, which indicates that the once-daily treatment therapy may have potential value in the clinical use of aminoglycosides. 相似文献
998.
We investigated the potential efficacy of treating adult T-cell leukemia (ATL) using a gene therapeutic approach involving the use of a herpes simplex virus-thymidine kinase (HSV-TK)-mediated suicide system. Human immunodeficiency virus (HIV)-based vectors containing the HSV-TK gene were constructed to achieve targeted gene transfer into CD4-positive ATL cells, after which the transduced cells were selectively killed by treatment with ganciclovir (GCV). To examine the utility of HIV vectors in vivo, ATL-NOD-SCID mice were prepared by intraperitoneal injection of 1 x 10(7) MT2 cells into NK-depleted nonobese diabetic/severely compromised immunodeficient (NOD-SCID) mice. Thereafter, 1 ml of concentrated HIV vector expressing HSV-TK (HXCTKN) or GFP (HXGFP) stock was injected into the intraperitoneal cavity, and GCV was administered twice a day for 5 days. Fluorescence-activated cell sorting (FACS) analysis showed that 7-11% of MT2 or HUT102 cells recovered from the peritoneal cavity were transduced with the HXGFP. After 3 weeks, plasma sIL2-R alpha levels were significantly lower in mice administered HXCTKN than in those administered HXGFP. Moreover, HXCTKN-injected mice survived significantly longer than HXGFP-injected mice. Taken together, these findings suggest that HIV vectors could be used for in vivo targeted gene transfer into ATL cells and could thus serve as the basis for the development of effective new therapies for the treatment of ATL. 相似文献
999.
1000.
Inhibitory effect of cephalosporins on gamma-aminobutyric acid receptor binding in rat synaptic membranes.
下载免费PDF全文
![点击此处可从《Antimicrobial agents and chemotherapy》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Cephalosporins inhibited gamma-aminobutyric acid receptor binding in a concentration-dependent manner in vitro. Scatchard analysis revealed that cefazolin decreased the binding capacity but did not change the affinity of the receptor. It is suggested that this inhibition of gamma-aminobutyric acid receptor binding may be involved in the induction of convulsions by cephalosporins. 相似文献